EY735088

Overview
NameEY735088
Unique NameEY735088
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1026
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY735088 vs. ExPASy Swiss-Prot
Match: PDXS_CORGL (Pyridoxal biosynthesis lyase pdxS OS=Corynebacterium glutamicum GN=pdxS PE=1 SV=2)

HSP 1 Score: 113.235 bits (282), Expect = 8.571e-40
Identity = 57/101 (56.44%), Postives = 75/101 (74.26%), Query Frame = 1 
Query:  331 NLGEALRRIREGAAMIRIQGDLLNSGNIVETVKNVRSLMGDIRILNNMDEDEVFTFAKKIAAPYDLVAQTKQMGRLPVVQFAAGGVVTPADAALMMAVGCE 633
            NLGEALRRI EGAAMIR +G+   +G++ E V+++R++ GDI  L ++DEDE+F  AK+  APYDLV +    G+LPVV F AGGV TPADAAL+  +G E
Sbjct:  154 NLGEALRRITEGAAMIRSKGEA-GTGDVSEAVRHLRTIRGDINRLRSLDEDELFVAAKEFQAPYDLVREVASTGKLPVVTFVAGGVATPADAALVRQMGAE 253

HSP 2 Score: 72.4034 bits (176), Expect = 8.571e-40
Identity = 36/75 (48.00%), Postives = 53/75 (70.67%), Query Frame = 2 
Query:  128 IVEVSSVEQAKIAEAAGACSIMVSEP------SRPGIKRMPDPSLVKSVKRAVSVPVMSRVRVGHFVEAQILERI 334
            I++V + EQA+IAE AGA ++M  E       S+ G+ RM DP L++ +  AVS+PVM++ R+GHFVEAQ+LE +
Sbjct:   45 IMDVVTPEQARIAEDAGASAVMALERVPADIRSQGGVARMSDPDLIEGIVNAVSIPVMAKARIGHFVEAQVLEAL 119
[Close]
BLAST of EY735088 vs. ExPASy Swiss-Prot
Match: PDXS_CLOCE (Pyridoxal biosynthesis lyase pdxS OS=Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) GN=pdxS PE=3 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 8.583e-40
Identity = 54/102 (52.94%), Postives = 77/102 (75.49%), Query Frame = 1 
Query:  328 KNLGEALRRIREGAAMIRIQGDLLNSGNIVETVKNVRSLMGDIRILNNMDEDEVFTFAKKIAAPYDLVAQTKQMGRLPVVQFAAGGVVTPADAALMMAVGCE 633
            KNLGEALRRI EGA+MIR +G+   +GN+VE V+++R++  +IR + +  + E+ T AK+  APYDL+    + G+LPV+ FAAGG+ TPADAALMM +GC+
Sbjct:  128 KNLGEALRRISEGASMIRTKGEA-GTGNVVEAVRHMRTVTNEIRKVQSASKQELMTIAKEFGAPYDLILYVHENGKLPVINFAAGGIATPADAALMMQLGCD 228

HSP 2 Score: 70.8626 bits (172), Expect = 8.583e-40
Identity = 35/75 (46.67%), Postives = 55/75 (73.33%), Query Frame = 2 
Query:  128 IVEVSSVEQAKIAEAAGACSIMVSE--PS----RPGIKRMPDPSLVKSVKRAVSVPVMSRVRVGHFVEAQILERI 334
            I++V + ++A+IA+ AGA ++M  E  PS      G+ RM DP ++K ++ AVS+PVM++VR+GHFVEAQ+LE +
Sbjct:   20 IMDVVNAKEAEIAQKAGAVAVMALERVPSDIRKAGGVARMSDPKMIKDIQSAVSIPVMAKVRIGHFVEAQVLEAL 94
[Close]
BLAST of EY735088 vs. ExPASy Swiss-Prot
Match: PDXS_METTH (Pyridoxal biosynthesis lyase pdxS OS=Methanobacterium thermoautotrophicum GN=pdxS PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.118e-39
Identity = 58/102 (56.86%), Postives = 81/102 (79.41%), Query Frame = 1 
Query:  328 KNLGEALRRIREGAAMIRIQGDLLNSGNIVETVKNVRSLMGDIRILNNMDEDEVFTFAKKIAAPYDLVAQTKQMGRLPVVQFAAGGVVTPADAALMMAVGCE 633
            +NLGEALRRI EGAAMIR +G+   +GNIVE V+++R +M +IR + N +E+E++  ++KI AP +LV +T ++G+LPVV FAAGGV TPADAALMM +G +
Sbjct:  129 RNLGEALRRIDEGAAMIRTKGEP-GTGNIVEAVRHMRIMMSEIREIQNKEEEELWEVSRKIEAPLELVRETAKLGKLPVVNFAAGGVATPADAALMMQLGAD 229

HSP 2 Score: 68.9366 bits (167), Expect = 1.118e-39
Identity = 35/75 (46.67%), Postives = 52/75 (69.33%), Query Frame = 2 
Query:  128 IVEVSSVEQAKIAEAAGACSIMVSEP------SRPGIKRMPDPSLVKSVKRAVSVPVMSRVRVGHFVEAQILERI 334
            I++V + EQA IAE +GA ++M  E       +  G+ RM DP+ V+ +  AVS+PVM++VR+GHFVEAQ+LE +
Sbjct:   21 IMDVVNAEQAAIAEDSGAVAVMALEKVPADIRASGGVARMADPNKVQEIMDAVSIPVMAKVRIGHFVEAQVLEAL 95
[Close]
BLAST of EY735088 vs. ExPASy Swiss-Prot
Match: PDXS_CORDI (Pyridoxal biosynthesis lyase pdxS OS=Corynebacterium diphtheriae GN=pdxS PE=3 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.456e-39
Identity = 57/101 (56.44%), Postives = 76/101 (75.25%), Query Frame = 1 
Query:  331 NLGEALRRIREGAAMIRIQGDLLNSGNIVETVKNVRSLMGDIRILNNMDEDEVFTFAKKIAAPYDLVAQTKQMGRLPVVQFAAGGVVTPADAALMMAVGCE 633
            NLGEALRRI EGAAMIR +G+   +G++ E VK++R++ G+I  L +MDED+++  AK+I APYDLV +    G+LPV  F AGGV TPADAAL+M +G E
Sbjct:  134 NLGEALRRITEGAAMIRSKGEA-GTGDVSEAVKHLRTIRGEINRLRSMDEDQLYVAAKEIQAPYDLVREVAATGKLPVTLFVAGGVATPADAALVMQMGAE 233

HSP 2 Score: 71.633 bits (174), Expect = 1.456e-39
Identity = 36/75 (48.00%), Postives = 53/75 (70.67%), Query Frame = 2 
Query:  128 IVEVSSVEQAKIAEAAGACSIMVSEP------SRPGIKRMPDPSLVKSVKRAVSVPVMSRVRVGHFVEAQILERI 334
            I++V + EQA+IAE AGA ++M  E       ++ G+ RM DP L++ +  AVS+PVM++ R+GHFVEAQILE +
Sbjct:   25 IMDVVTPEQARIAEDAGASAVMALERVPADIRAQGGVARMSDPDLIEGIVNAVSIPVMAKARIGHFVEAQILESL 99
[Close]
BLAST of EY735088 vs. ExPASy Swiss-Prot
Match: PDXS_BIFLO (Pyridoxal biosynthesis lyase pdxS OS=Bifidobacterium longum GN=pdxS PE=3 SV=2)

HSP 1 Score: 110.538 bits (275), Expect = 1.457e-39
Identity = 57/102 (55.88%), Postives = 75/102 (73.53%), Query Frame = 1 
Query:  328 KNLGEALRRIREGAAMIRIQGDLLNSGNIVETVKNVRSLMGDIRILNNMDEDEVFTFAKKIAAPYDLVAQTKQMGRLPVVQFAAGGVVTPADAALMMAVGCE 633
            KNLGEALRRI EGAAMIR +G+   +G++++ V+++R++   IR L  + +DEV+  AK++A PYDL       GRLPVV FAAGGV TPADAALMM +G E
Sbjct:  129 KNLGEALRRIAEGAAMIRTKGEP-GTGDVIQAVRHMRTMNKQIRELVGLRDDEVYEAAKQLAVPYDLAKYVHDNGRLPVVNFAAGGVATPADAALMMELGAE 229

HSP 2 Score: 74.3294 bits (181), Expect = 1.457e-39
Identity = 35/75 (46.67%), Postives = 55/75 (73.33%), Query Frame = 2 
Query:  128 IVEVSSVEQAKIAEAAGACSIMVSEP------SRPGIKRMPDPSLVKSVKRAVSVPVMSRVRVGHFVEAQILERI 334
            I++V++ EQAKIA+ AGAC++M  E       +  G+ RM DP+++K ++ AVS+PVM++VR+GH  EA+IL+ I
Sbjct:   21 IMDVTTPEQAKIAQDAGACAVMALERIPADIRAAGGVSRMSDPAMIKGIQEAVSIPVMAKVRIGHIAEARILQAI 95
[Close]
BLAST of EY735088 vs. ExPASy Swiss-Prot
Match: PDXS_BIFLI (Pyridoxal biosynthesis lyase pdxS OS=Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / NCTC 11817 / S12) GN=pdxS PE=3 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.457e-39
Identity = 57/102 (55.88%), Postives = 75/102 (73.53%), Query Frame = 1 
Query:  328 KNLGEALRRIREGAAMIRIQGDLLNSGNIVETVKNVRSLMGDIRILNNMDEDEVFTFAKKIAAPYDLVAQTKQMGRLPVVQFAAGGVVTPADAALMMAVGCE 633
            KNLGEALRRI EGAAMIR +G+   +G++++ V+++R++   IR L  + +DEV+  AK++A PYDL       GRLPVV FAAGGV TPADAALMM +G E
Sbjct:  129 KNLGEALRRIAEGAAMIRTKGEP-GTGDVIQAVRHMRTMNKQIRELVGLRDDEVYEAAKQLAVPYDLAKYVHDNGRLPVVNFAAGGVATPADAALMMELGAE 229

HSP 2 Score: 74.3294 bits (181), Expect = 1.457e-39
Identity = 35/75 (46.67%), Postives = 55/75 (73.33%), Query Frame = 2 
Query:  128 IVEVSSVEQAKIAEAAGACSIMVSEP------SRPGIKRMPDPSLVKSVKRAVSVPVMSRVRVGHFVEAQILERI 334
            I++V++ EQAKIA+ AGAC++M  E       +  G+ RM DP+++K ++ AVS+PVM++VR+GH  EA+IL+ I
Sbjct:   21 IMDVTTPEQAKIAQDAGACAVMALERIPADIRAAGGVSRMSDPAMIKGIQEAVSIPVMAKVRIGHIAEARILQAI 95
[Close]
BLAST of EY735088 vs. ExPASy Swiss-Prot
Match: PDXS_DESHY (Pyridoxal biosynthesis lyase pdxS OS=Desulfitobacterium hafniense (strain Y51) GN=pdxS PE=3 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 1.457e-39
Identity = 57/102 (55.88%), Postives = 72/102 (70.59%), Query Frame = 1 
Query:  328 KNLGEALRRIREGAAMIRIQGDLLNSGNIVETVKNVRSLMGDIRILNNMDEDEVFTFAKKIAAPYDLVAQTKQMGRLPVVQFAAGGVVTPADAALMMAVGCE 633
            K+LGEALRRI EGAAMIR +G+   +G+IV+ V+++R +   I  L  M EDE+F  AK++  PYDLV    +  RLPVV FAAGGV TP DAALMM +G E
Sbjct:  129 KDLGEALRRINEGAAMIRTKGEP-GTGDIVQAVRHMRMINRQISRLAGMREDELFQEAKELQVPYDLVLYVHEHKRLPVVNFAAGGVATPGDAALMMQLGAE 229

HSP 2 Score: 80.1073 bits (196), Expect = 1.457e-39
Identity = 39/75 (52.00%), Postives = 55/75 (73.33%), Query Frame = 2 
Query:  128 IVEVSSVEQAKIAEAAGACSIMVSEP------SRPGIKRMPDPSLVKSVKRAVSVPVMSRVRVGHFVEAQILERI 334
            I++V++ EQAKIAEAAGAC++M  E       +  G+ RM DP ++K +  AVS+PVM++ R+GHFVEAQILE +
Sbjct:   21 IMDVTTPEQAKIAEAAGACAVMALERIPADIRAVGGVSRMSDPKMIKGIMEAVSIPVMAKCRIGHFVEAQILEAV 95
[Close]
BLAST of EY735088 vs. ExPASy Swiss-Prot
Match: PDXS_DESHD (Pyridoxal biosynthesis lyase pdxS OS=Desulfitobacterium hafniense (strain DCB-2 / DSM 10664) GN=pdxS PE=3 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 1.457e-39
Identity = 57/102 (55.88%), Postives = 72/102 (70.59%), Query Frame = 1 
Query:  328 KNLGEALRRIREGAAMIRIQGDLLNSGNIVETVKNVRSLMGDIRILNNMDEDEVFTFAKKIAAPYDLVAQTKQMGRLPVVQFAAGGVVTPADAALMMAVGCE 633
            K+LGEALRRI EGAAMIR +G+   +G+IV+ V+++R +   I  L  M EDE+F  AK++  PYDLV    +  RLPVV FAAGGV TP DAALMM +G E
Sbjct:  129 KDLGEALRRINEGAAMIRTKGEP-GTGDIVQAVRHMRMINRQISRLAGMREDELFQEAKELQVPYDLVLYVHEHKRLPVVNFAAGGVATPGDAALMMQLGAE 229

HSP 2 Score: 80.1073 bits (196), Expect = 1.457e-39
Identity = 39/75 (52.00%), Postives = 55/75 (73.33%), Query Frame = 2 
Query:  128 IVEVSSVEQAKIAEAAGACSIMVSEP------SRPGIKRMPDPSLVKSVKRAVSVPVMSRVRVGHFVEAQILERI 334
            I++V++ EQAKIAEAAGAC++M  E       +  G+ RM DP ++K +  AVS+PVM++ R+GHFVEAQILE +
Sbjct:   21 IMDVTTPEQAKIAEAAGACAVMALERIPADIRAVGGVSRMSDPKMIKGIMEAVSIPVMAKCRIGHFVEAQILEAV 95
[Close]
BLAST of EY735088 vs. ExPASy Swiss-Prot
Match: PDXS_DESDA (Pyridoxal biosynthesis lyase pdxS OS=Desulfovibrio desulfuricans (strain ATCC 27774 / DSM 6949) GN=pdxS PE=3 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 1.898e-39
Identity = 56/102 (54.90%), Postives = 77/102 (75.49%), Query Frame = 1 
Query:  328 KNLGEALRRIREGAAMIRIQGDLLNSGNIVETVKNVRSLMGDIRILNNMDEDEVFTFAKKIAAPYDLVAQTKQMGRLPVVQFAAGGVVTPADAALMMAVGCE 633
            +NLGEALRRI EGAAMIR +G+   +GN+VE V++ R +M ++R+L  + E EV  FAK++ AP +L    ++ GRLPVV FAAGG+ TPADAA+MM +GC+
Sbjct:  129 RNLGEALRRIAEGAAMIRTKGEP-GTGNVVEAVRHCRQVMDEVRMLCALPEAEVPNFAKEMGAPLELCLLVRKEGRLPVVNFAAGGIATPADAAMMMHLGCD 229

HSP 2 Score: 73.1738 bits (178), Expect = 1.898e-39
Identity = 35/73 (47.95%), Postives = 52/73 (71.23%), Query Frame = 2 
Query:  128 IVEVSSVEQAKIAEAAGACSIMVSEP------SRPGIKRMPDPSLVKSVKRAVSVPVMSRVRVGHFVEAQILE 328
            I++V++ EQAKIAE AGAC++M  E       +  G+ RM DP++VK +    ++PVM++ R+GHFVEA+ILE
Sbjct:   21 IMDVTTPEQAKIAEEAGACAVMALERVPADIRAAGGVARMADPTIVKKIMEVATIPVMAKARIGHFVEARILE 93
[Close]
BLAST of EY735088 vs. ExPASy Swiss-Prot
Match: PDXS_CLOBM (Pyridoxal biosynthesis lyase pdxS OS=Clostridium botulinum (strain Loch Maree / Type A3) GN=pdxS PE=3 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.898e-39
Identity = 52/102 (50.98%), Postives = 78/102 (76.47%), Query Frame = 1 
Query:  328 KNLGEALRRIREGAAMIRIQGDLLNSGNIVETVKNVRSLMGDIRILNNMDEDEVFTFAKKIAAPYDLVAQTKQMGRLPVVQFAAGGVVTPADAALMMAVGCE 633
            +NLGEALRRI EGA+MIR +G+   +GN+VE V+++R++  ++R L    ++E+ T AK++ AP++L+    + GRLPV+ FAAGG+ TPADAALMM +GC+
Sbjct:  128 RNLGEALRRIGEGASMIRTKGEA-GTGNVVEAVRHMRTISSEMRKLQLTPKEELMTVAKEMGAPFNLIEYVAEKGRLPVINFAAGGIATPADAALMMQLGCD 228

HSP 2 Score: 73.9442 bits (180), Expect = 1.898e-39
Identity = 40/75 (53.33%), Postives = 54/75 (72.00%), Query Frame = 2 
Query:  128 IVEVSSVEQAKIAEAAGACSIMVSE--PS----RPGIKRMPDPSLVKSVKRAVSVPVMSRVRVGHFVEAQILERI 334
            I++V + EQAKIAE AGA ++M  E  PS    + G+ R  DP ++K +  AVS+PVM++VR+GHFVEAQILE I
Sbjct:   20 IMDVVNPEQAKIAEEAGAVAVMALERVPSDIRKQGGVARTSDPKMIKEIINAVSIPVMAKVRIGHFVEAQILEAI 94
The following BLAST results are available for this feature:
BLAST of EY735088 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 207
Match NameE-valueIdentityDescription
PDXS_CORGL8.571e-4056.44Pyridoxal biosynthesis lyase pdxS OS=Corynebacteri... [more]
PDXS_CLOCE8.583e-4052.94Pyridoxal biosynthesis lyase pdxS OS=Clostridium c... [more]
PDXS_METTH1.118e-3956.86Pyridoxal biosynthesis lyase pdxS OS=Methanobacter... [more]
PDXS_CORDI1.456e-3956.44Pyridoxal biosynthesis lyase pdxS OS=Corynebacteri... [more]
PDXS_BIFLO1.457e-3955.88Pyridoxal biosynthesis lyase pdxS OS=Bifidobacteri... [more]
PDXS_BIFLI1.457e-3955.88Pyridoxal biosynthesis lyase pdxS OS=Bifidobacteri... [more]
PDXS_DESHY1.457e-3955.88Pyridoxal biosynthesis lyase pdxS OS=Desulfitobact... [more]
PDXS_DESHD1.457e-3955.88Pyridoxal biosynthesis lyase pdxS OS=Desulfitobact... [more]
PDXS_DESDA1.898e-3954.90Pyridoxal biosynthesis lyase pdxS OS=Desulfovibrio... [more]
PDXS_CLOBM1.898e-3950.98Pyridoxal biosynthesis lyase pdxS OS=Clostridium b... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-082-C02-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY735088 ID=EY735088; Name=EY735088; organism=Citrus sinensis; type=EST; length=1026bp
ANAAGAAGGGACGAGGGGGGGGANTGAANNNNNCCCAAANNNNNAAAANN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGCCGACCCACGCGTCCGC
GGACGCGNNNTGGTCGGACGCGTGGGCATTGTTGAAGTCTCCAGTGTGGA
ACAAGCCAAGATCGCCGAAGCAGCTGGCGCCTGTTCTATCATGGTATCCG
AGCCCAGCCGGCCCGGCATTAAGCGCATGCCGGATCCTTCTCTTGTTAAA
AGCGTTAAACGCGCCGTTTCAGTCCCCGTTATGTCACGTGTTCGTGTTGG
GCATTTTGTTGAAGCGCAGATTCTTGAAAGAATCTTGGTGAAGCTTTGAG
GAGAATTAGAGAAGGAGCTGCTATGATAAGAATACAAGGGGATTTGTTAA
ATTCTGGTAATATTGTCGAGACTGTTAAAAATGTGAGGTCGTTAATGGGA
GATATTCGAATTTTGAATAACATGGATGAAGATGAGGTCTTCACGTTTGC
TAAGAAGATAGCGGCTCCGTATGATTTGGTGGCTCAAACTAAACAAATGG
GAAGGTTGCCCGTGGTGCAATTTGCTGCAGGAGGGGTTGTGACACCTGCT
GATGCCGCCCTGATGATGGCAGTGGGATGTGAATGGGGTGGTTGTGGGAA
TCGAGGTTTTTGAGTGCTCAAATCCGCACAAGGAAGTGGAGGGGGTATGG
GCGCCGCCTGTTAGGCCTAAAAACTGGACCCGCCGCGGCTCGGGGGGAAG
GGGCACGTGCGCGATAGCACGAACGCGCATTGCGGGGGAAGTCGCCGGGC
GGTGAGGAAGGAACCAGAAATGGAAGGTCGCAAAGAAGCGAGGTAACGGA
ATGAGCCCCCCATAAGGCCCCCTCCCGTCGCTCCTGCCCCCATCTCGCCC
CACNCTCGCCACCACAGGGCGGGCGCGGAGGCGCGGGCGGGCATGGCGTG
ACGCCGGGCCCCGGCGGTGCCGCCGTCGCGGGTGACCGGCCGCCGCGCCG
GACGCTCGCCTCGCGGTGGCGGAGGG
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