EY734946

Overview
NameEY734946
Unique NameEY734946
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length917
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY734946 vs. ExPASy Swiss-Prot
Match: UBIQ_LEIMA (Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 3.213e-33
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = 3 
Query:  204 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 431
            MQIFVKTLTGKTI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 120.168 bits (300), Expect = 1.708e-26
Identity = 59/66 (89.39%), Postives = 63/66 (95.45%), Query Frame = 3 
Query:    6 RPITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 203
            + I LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:   11 KTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76
[Close]
BLAST of EY734946 vs. ExPASy Swiss-Prot
Match: UBIQ_LEITA (Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 1.034e-31
Identity = 69/76 (90.79%), Postives = 72/76 (94.74%), Query Frame = 3 
Query:  204 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 431
            MQIFVKTLTG TI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGTTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 117.087 bits (292), Expect = 1.446e-25
Identity = 58/64 (90.62%), Postives = 61/64 (95.31%), Query Frame = 3 
Query:   12 ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 203
            I LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:   13 IALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76
[Close]
BLAST of EY734946 vs. ExPASy Swiss-Prot
Match: UBIL_NPVOP (Ubiquitin-like protein OS=Orgyia pseudotsugata multicapsid polyhedrosis virus GN=V-UBI PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 5.308e-28
Identity = 61/76 (80.26%), Postives = 67/76 (88.16%), Query Frame = 3 
Query:  204 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 431
            MQIFVKTLTGKTIT+E E  DT+  VK KI DKEG+P DQQRLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFVKTLTGKTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGG 76

HSP 2 Score: 102.834 bits (255), Expect = 2.821e-21
Identity = 49/66 (74.24%), Postives = 56/66 (84.85%), Query Frame = 3 
Query:    6 RPITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 203
            + IT+E E  DT+  VK KI DKEG+P DQQRLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG
Sbjct:   11 KTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGG 76
[Close]
BLAST of EY734946 vs. ExPASy Swiss-Prot
Match: UBIL_NPVAC (Ubiquitin-like protein OS=Autographa californica nuclear polyhedrosis virus GN=V-UBI PE=3 SV=2)

HSP 1 Score: 123.635 bits (309), Expect = 1.545e-27
Identity = 58/77 (75.32%), Postives = 69/77 (89.61%), Query Frame = 3 
Query:  204 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGF 434
            MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG+
Sbjct:    1 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGGY 77

HSP 2 Score: 100.523 bits (249), Expect = 1.400e-20
Identity = 47/66 (71.21%), Postives = 57/66 (86.36%), Query Frame = 3 
Query:    6 RPITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 203
            + IT E E ++T+ ++K KI DKEG+P DQQRLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG
Sbjct:   11 KTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76
[Close]
BLAST of EY734946 vs. ExPASy Swiss-Prot
Match: UBIQ_LUMTE (Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 SV=2)

HSP 1 Score: 120.553 bits (301), Expect = 1.308e-26
Identity = 60/63 (95.24%), Postives = 62/63 (98.41%), Query Frame = 3 
Query:  237 TITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 425
            TITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:    1 TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63

HSP 2 Score: 118.627 bits (296), Expect = 4.969e-26
Identity = 59/62 (95.16%), Postives = 61/62 (98.39%), Query Frame = 3 
Query:   12 ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 197
            ITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:    2 ITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63
[Close]
BLAST of EY734946 vs. ExPASy Swiss-Prot
Match: RUB1_ARATH (NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN=RUB1 PE=1 SV=2)

HSP 1 Score: 101.293 bits (251), Expect = 8.209e-21
Identity = 48/75 (64.00%), Postives = 60/75 (80.00%), Query Frame = 3 
Query:  210 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGF 434
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGGF
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGGF 77

HSP 2 Score: 86.2705 bits (212), Expect = 2.733e-16
Identity = 39/66 (59.09%), Postives = 52/66 (78.79%), Query Frame = 3 
Query:    6 RPITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 203
            + I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:   11 KEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76
[Close]
BLAST of EY734946 vs. ExPASy Swiss-Prot
Match: RUB1_DESAN (NEDD8-like protein RUB1 OS=Deschampsia antarctica GN=RUB1 PE=2 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 1.400e-20
Identity = 48/75 (64.00%), Postives = 60/75 (80.00%), Query Frame = 3 
Query:  210 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGF 434
            I VKTLTGK I +++E +DTID VK ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRVKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGGY 77

HSP 2 Score: 86.6557 bits (213), Expect = 2.092e-16
Identity = 40/66 (60.61%), Postives = 52/66 (78.79%), Query Frame = 3 
Query:    6 RPITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 203
            + I +++E +DTID VK ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:   11 KEIEIDIEPTDTIDRVKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76
[Close]
BLAST of EY734946 vs. ExPASy Swiss-Prot
Match: RUB1_ORYSJ (NEDD8-like protein RUB1 OS=Oryza sativa subsp. japonica GN=RUB1 PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.829e-20
Identity = 47/75 (62.67%), Postives = 60/75 (80.00%), Query Frame = 3 
Query:  210 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGF 434
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGGY 77

HSP 2 Score: 86.2705 bits (212), Expect = 2.733e-16
Identity = 39/66 (59.09%), Postives = 52/66 (78.79%), Query Frame = 3 
Query:    6 RPITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 203
            + I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:   11 KEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76
[Close]
BLAST of EY734946 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 3.119e-20
Identity = 47/77 (61.04%), Postives = 59/77 (76.62%), Query Frame = 3 
Query:  204 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGF 434
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGGF
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGGF 77

HSP 2 Score: 83.1889 bits (204), Expect = 2.313e-15
Identity = 37/66 (56.06%), Postives = 50/66 (75.76%), Query Frame = 3 
Query:    6 RPITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 203
            + I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:   11 KEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76
[Close]
BLAST of EY734946 vs. ExPASy Swiss-Prot
Match: RUB2_ORYSJ (NEDD8-like protein RUB2 OS=Oryza sativa subsp. japonica GN=RUB2 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 4.074e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 3 
Query:  210 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 431
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 86.2705 bits (212), Expect = 2.733e-16
Identity = 39/66 (59.09%), Postives = 52/66 (78.79%), Query Frame = 3 
Query:    6 RPITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 203
            + I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:   11 KEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76
The following BLAST results are available for this feature:
BLAST of EY734946 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 98
Match NameE-valueIdentityDescription
UBIQ_LEIMA3.213e-3393.42Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1[more]
UBIQ_LEITA1.034e-3190.79Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3... [more]
UBIL_NPVOP5.308e-2880.26Ubiquitin-like protein OS=Orgyia pseudotsugata mul... [more]
UBIL_NPVAC1.545e-2775.32Ubiquitin-like protein OS=Autographa californica n... [more]
UBIQ_LUMTE1.308e-2695.24Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 ... [more]
RUB1_ARATH8.209e-2164.00NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN... [more]
RUB1_DESAN1.400e-2064.00NEDD8-like protein RUB1 OS=Deschampsia antarctica ... [more]
RUB1_ORYSJ1.829e-2062.67NEDD8-like protein RUB1 OS=Oryza sativa subsp. jap... [more]
NEDD8_CAEEL3.119e-2061.04NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1[more]
RUB2_ORYSJ4.074e-2063.51NEDD8-like protein RUB2 OS=Oryza sativa subsp. jap... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-108-D02-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY734946 ID=EY734946; Name=EY734946; organism=Citrus sinensis; type=EST; length=917bp
CCACGCGTCCGATTACTCTTGAGGTTGAAAGTTCTGACACCATTGACAAC
GTGAAAGCAAAAATTCAAGACAAGGAGGGTATCCCACCAGATCAGCAGAG
GCTCATTTTTGCTGGAAAGCAGCTTGAAGATGGAAGGACTCTTGCAGACT
ATAACATCCAGAAAGAATCCACTCTTCACTTGGTTCTTCGCCTCCGTGGT
GGTATGCAGATTTTTGTGAAGACACTCACTGGGAAAACCATCACGTTGGA
GGTTGAGAGTTCGGACACAATTGACAATGTGAAGGCTAAGATCCAGGATA
AGGAGGGGATTCCCCCAGACCAGCAGAGGTTGATCTTTGCTGGAAAACAA
CTTGAGGATGGAAGGACTCTTGCTGATTACAACATCCAGAAGGAGTCCAC
TCTGCATCTTGTGCTCCGACTCCGTGGAGGTTTCTGAGTTTGGTCATCTG
GGTGTGTGTGTGTCTTATTTGCTTCGGTTTGTCTTGGTTAATGTTTGCTG
GCTATTGGTGAAATAGCTTANATAACTATTTGAAGTTTATTTTGGTTTGT
GGTAGTTGAACTCTGTTTCTAAGATATTGATATGGGCCCACTAACGTCCC
ATTATTATATCTTAGAATCTTTTTCCTGGAAAAATACGTGGTTAATGGGC
GAGACTTAAAGAAAAAAACAAAAAAAAAGGGGCGGCCGCTCTGAAAGATC
CCAGGCTTACTAACGCGGGGAGGGCACGGCATAAGCTCCTCATGAGGGGG
CCCCTAAATACAATTTCGCTGGGCCGCCGGTTTAAAAGCTACCGGAACTG
GGAAAAACCCTCGGCGGTGGCGCGAGGTAAAACGCGGTTGGCAAGAGGAC
CCCGCCTTTCCGCCGAGGCGGGCGGAGAAAAGGGAAGAGGAGGGCCCGAC
GGGGGGTGCCGCCTTCC
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