EY734857 | Citrus Genome Database

Overview


Name	EY734857
Unique Name	EY734857
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	820

Libraries


Library Name	Type
Sweet orange fruit, development stadium (5 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY734857 vs. ExPASy Swiss-Prot
Match: LLDD_PSEAB (L-lactate dehydrogenase [cytochrome] OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=lldD PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 1.102e-17
Identity = 40/101 (39.60%), Postives = 61/101 (60.40%), Query Frame = 3

Query:  180 VKGVLTPQDARIA*SAEATGIIVSNHGPRQLDYVPATIMALEEVVKATHGRIPVLLDGGVKRGTDVFKTLAPEASDIFIGRPAAYSLTSAGHICLKTVLHM 482
            +KG+L P+DAR A    A GI+VSNHG RQLD V ++  AL  +  A  G + +L D G++ G DV + +A  A  + +GR   Y+L +AG   ++ +L +
Sbjct:  250 IKGILDPEDARDAVKFGADGIVVSNHGGRQLDGVLSSARALPAIADAVKGELAILADSGIRTGLDVVRMIALGADSVLLGRAFVYALAAAGEAGVRNLLEL 350

HSP 2 Score: 30.8018 bits (68), Expect = 1.102e-17
Identity = 15/50 (30.00%), Postives = 27/50 (54.00%), Query Frame = 2

Query:   53 DLGKMD--EANDSGLAAYVA---GQIDRSLSWKDVKWLQTITKLPILSEG 187
            DLG +     N +GL  Y+       D S++WKD++W++     P++ +G
Sbjct:  203 DLGNISAYRGNPTGLEDYIGWLGANFDPSIAWKDLEWIREFWDGPMVIKG 252

[Close]

BLAST of EY734857 vs. ExPASy Swiss-Prot
Match: LLDD_ALCFA (L-lactate dehydrogenase [cytochrome] OS=Alcaligenes faecalis GN=lldD PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.102e-17
Identity = 46/123 (37.40%), Postives = 67/123 (54.47%), Query Frame = 3

Query:  180 VKGVLTPQDARIA*SAEATGIIVSNHGPRQLDYVPATIMALEEVVKATHGRIPVLLDGGVKRGTDVFKTLAPEASDIFIGRPAAYSLTSAGHICLKTVLHMHNRKYHLPTDLNMPSMIQPKTA 548
            +KG+L P DAR A    A GIIVSNHG RQLD V ++  AL  +  A  G+I +L D G++ G DV + +A  A    +GR   Y+L +AG   +  +L +  ++  +   L   S I   T+
Sbjct:  250 IKGILDPDDARDAVRFGADGIIVSNHGGRQLDGVLSSARALPAIADAVKGQIKILADSGIRSGLDVVRMIALGADAAMLGRAYIYALAAAGQSGVDHLLGLIEKEIRVAMTLTSVSSISQITS 372

HSP 2 Score: 33.4982 bits (75), Expect = 1.102e-17
Identity = 12/30 (40.00%), Postives = 20/30 (66.67%), Query Frame = 2

Query:   98 YVAGQIDRSLSWKDVKWLQTITKLPILSEG 187
            Y+    D S+SWKD++W++   K P+L +G
Sbjct:  223 YLGANFDPSISWKDLEWIREFWKGPMLIKG 252

[Close]

BLAST of EY734857 vs. ExPASy Swiss-Prot
Match: LLDD_XANCP (L-lactate dehydrogenase [cytochrome] OS=Xanthomonas campestris pv. campestris GN=lldD PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 2.421e-17
Identity = 41/105 (39.05%), Postives = 62/105 (59.05%), Query Frame = 3

Query:  180 VKGVLTPQDARIA*SAEATGIIVSNHGPRQLDYVPATIMALEEVVKATHGRIPVLLDGGVKRGTDVFKTLAPEASDIFIGRPAAYSLTSAGHICLKTVLHMHNRK 494
            +KG+L P+DAR A    A GI+VSNHG RQLD V ++  AL  +  A  G + +L D G++ G DV + LA  A  + +GR   Y+L + G   ++ +L +  R+
Sbjct:  250 IKGILDPEDARDAVRFGADGIVVSNHGGRQLDGVLSSARALPAIADAVKGELKILADSGIRSGLDVVRMLALGADAVLLGRAFVYALAAGGQAGVENLLTLIERE 354

HSP 2 Score: 31.5722 bits (70), Expect = 2.421e-17
Identity = 16/50 (32.00%), Postives = 28/50 (56.00%), Query Frame = 2

Query:   53 DLGKMD--EANDSGLAAYV---AGQIDRSLSWKDVKWLQTITKLPILSEG 187
            DLG +     + +GL  Y+   A   D S+SWKD++W++     P++ +G
Sbjct:  203 DLGNISTYRGSPTGLQDYIGWLAANFDPSISWKDLEWIREFWTGPMVIKG 252

[Close]

BLAST of EY734857 vs. ExPASy Swiss-Prot
Match: LLDD_XANCB (L-lactate dehydrogenase [cytochrome] OS=Xanthomonas campestris pv. campestris (strain B100) GN=lldD PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 2.421e-17
Identity = 41/105 (39.05%), Postives = 62/105 (59.05%), Query Frame = 3

Query:  180 VKGVLTPQDARIA*SAEATGIIVSNHGPRQLDYVPATIMALEEVVKATHGRIPVLLDGGVKRGTDVFKTLAPEASDIFIGRPAAYSLTSAGHICLKTVLHMHNRK 494
            +KG+L P+DAR A    A GI+VSNHG RQLD V ++  AL  +  A  G + +L D G++ G DV + LA  A  + +GR   Y+L + G   ++ +L +  R+
Sbjct:  250 IKGILDPEDARDAVRFGADGIVVSNHGGRQLDGVLSSARALPAIADAVKGELKILADSGIRSGLDVVRMLALGADAVLLGRAFVYALAAGGQAGVENLLTLIERE 354

HSP 2 Score: 31.5722 bits (70), Expect = 2.421e-17
Identity = 16/50 (32.00%), Postives = 28/50 (56.00%), Query Frame = 2

Query:   53 DLGKMD--EANDSGLAAYV---AGQIDRSLSWKDVKWLQTITKLPILSEG 187
            DLG +     + +GL  Y+   A   D S+SWKD++W++     P++ +G
Sbjct:  203 DLGNISTYRGSPTGLQDYIGWLAANFDPSISWKDLEWIREFWTGPMVIKG 252

[Close]

BLAST of EY734857 vs. ExPASy Swiss-Prot
Match: LLDD_XANC8 (L-lactate dehydrogenase [cytochrome] OS=Xanthomonas campestris pv. campestris (strain 8004) GN=lldD PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 2.421e-17
Identity = 41/105 (39.05%), Postives = 62/105 (59.05%), Query Frame = 3

Query:  180 VKGVLTPQDARIA*SAEATGIIVSNHGPRQLDYVPATIMALEEVVKATHGRIPVLLDGGVKRGTDVFKTLAPEASDIFIGRPAAYSLTSAGHICLKTVLHMHNRK 494
            +KG+L P+DAR A    A GI+VSNHG RQLD V ++  AL  +  A  G + +L D G++ G DV + LA  A  + +GR   Y+L + G   ++ +L +  R+
Sbjct:  250 IKGILDPEDARDAVRFGADGIVVSNHGGRQLDGVLSSARALPAIADAVKGELKILADSGIRSGLDVVRMLALGADAVLLGRAFVYALAAGGQAGVENLLTLIERE 354

HSP 2 Score: 31.5722 bits (70), Expect = 2.421e-17
Identity = 16/50 (32.00%), Postives = 28/50 (56.00%), Query Frame = 2

Query:   53 DLGKMD--EANDSGLAAYV---AGQIDRSLSWKDVKWLQTITKLPILSEG 187
            DLG +     + +GL  Y+   A   D S+SWKD++W++     P++ +G
Sbjct:  203 DLGNISTYRGSPTGLQDYIGWLAANFDPSISWKDLEWIREFWTGPMVIKG 252

[Close]

BLAST of EY734857 vs. ExPASy Swiss-Prot
Match: LLDD_ECOUT (L-lactate dehydrogenase [cytochrome] OS=Escherichia coli (strain UTI89 / UPEC) GN=lldD PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 3.145e-17
Identity = 41/101 (40.59%), Postives = 61/101 (60.40%), Query Frame = 3

Query:  180 VKGVLTPQDARIA*SAEATGIIVSNHGPRQLDYVPATIMALEEVVKATHGRIPVLLDGGVKRGTDVFKTLAPEASDIFIGRPAAYSLTSAGHICLKTVLHM 482
            +KG+L P+DAR A    A GI+VSNHG RQLD V ++  AL  +  A  G I +L D G++ G DV + +A  A  + +GR   Y+L +AG   +  +L++
Sbjct:  250 IKGILDPEDARDAVRFGADGIVVSNHGGRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLYALATAGQAGVANLLNL 350

HSP 2 Score: 30.8018 bits (68), Expect = 3.145e-17
Identity = 16/50 (32.00%), Postives = 27/50 (54.00%), Query Frame = 2

Query:   53 DLGKMDE--ANDSGLAAYV---AGQIDRSLSWKDVKWLQTITKLPILSEG 187
            DLG +       +GL  Y+   A   D S+SWKD++W++     P++ +G
Sbjct:  203 DLGNISAYLGKPTGLEDYIGWLANNFDPSISWKDLEWIRDFWDGPMVIKG 252

[Close]

BLAST of EY734857 vs. ExPASy Swiss-Prot
Match: LLDD_ECOK1 (L-lactate dehydrogenase [cytochrome] OS=Escherichia coli O1:K1 / APEC GN=lldD PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 3.145e-17
Identity = 41/101 (40.59%), Postives = 61/101 (60.40%), Query Frame = 3

Query:  180 VKGVLTPQDARIA*SAEATGIIVSNHGPRQLDYVPATIMALEEVVKATHGRIPVLLDGGVKRGTDVFKTLAPEASDIFIGRPAAYSLTSAGHICLKTVLHM 482
            +KG+L P+DAR A    A GI+VSNHG RQLD V ++  AL  +  A  G I +L D G++ G DV + +A  A  + +GR   Y+L +AG   +  +L++
Sbjct:  250 IKGILDPEDARDAVRFGADGIVVSNHGGRQLDGVLSSACALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLYALATAGQAGVANLLNL 350

HSP 2 Score: 30.8018 bits (68), Expect = 3.145e-17
Identity = 16/50 (32.00%), Postives = 27/50 (54.00%), Query Frame = 2

Query:   53 DLGKMDE--ANDSGLAAYV---AGQIDRSLSWKDVKWLQTITKLPILSEG 187
            DLG +       +GL  Y+   A   D S+SWKD++W++     P++ +G
Sbjct:  203 DLGNISAYLGKPTGLEDYIGWLANNFDPSISWKDLEWIRDFWDGPMVIKG 252

[Close]

BLAST of EY734857 vs. ExPASy Swiss-Prot
Match: LLDD_ECO45 (L-lactate dehydrogenase [cytochrome] OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=lldD PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 3.145e-17
Identity = 41/101 (40.59%), Postives = 61/101 (60.40%), Query Frame = 3

Query:  180 VKGVLTPQDARIA*SAEATGIIVSNHGPRQLDYVPATIMALEEVVKATHGRIPVLLDGGVKRGTDVFKTLAPEASDIFIGRPAAYSLTSAGHICLKTVLHM 482
            +KG+L P+DAR A    A GI+VSNHG RQLD V ++  AL  +  A  G I +L D G++ G DV + +A  A  + +GR   Y+L +AG   +  +L++
Sbjct:  250 IKGILDPEDARDAVRFGADGIVVSNHGGRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLYALATAGQAGVANLLNL 350

HSP 2 Score: 30.8018 bits (68), Expect = 3.145e-17
Identity = 16/50 (32.00%), Postives = 27/50 (54.00%), Query Frame = 2

Query:   53 DLGKMDE--ANDSGLAAYV---AGQIDRSLSWKDVKWLQTITKLPILSEG 187
            DLG +       +GL  Y+   A   D S+SWKD++W++     P++ +G
Sbjct:  203 DLGNISAYLGKPTGLEDYIGWLANNFDPSISWKDLEWIRDFWDGPMVIKG 252

[Close]

BLAST of EY734857 vs. ExPASy Swiss-Prot
Match: CYB2_HANAN (Cytochrome b2, mitochondrial OS=Hansenula anomala GN=CYB2 PE=1 SV=2)

HSP 1 Score: 72.7886 bits (177), Expect = 4.054e-17
Identity = 38/96 (39.58%), Postives = 56/96 (58.33%), Query Frame = 3

Query:  180 VKGVLTPQDARIA*SAEATGIIVSNHGPRQLDYVPATIMALEEVV-----KATHGRIPVLLDGGVKRGTDVFKTLAPEASDIFIGRPAAYSLTSAG 452
            +KGV   +D  +A      G+++SNHG RQLDY  A +  L EV+     +    +I + +DGGV+RGTDV K L   A  + +GRP  Y+++S G
Sbjct:  407 IKGVQRKEDVLLAAEHGLQGVVLSNHGGRQLDYTRAPVEVLAEVMPILKERGLDQKIDIFVDGGVRRGTDVLKALCLGAKGVGLGRPFLYAMSSYG 502

HSP 2 Score: 37.3502 bits (85), Expect = 4.054e-17
Identity = 16/40 (40.00%), Postives = 27/40 (67.50%), Query Frame = 2

Query:   68 DEANDSGLAAYVAGQIDRSLSWKDVKWLQTITKLPILSEG 187
            D     G +  ++  ID SLSWKD+ ++++ITK+PI+ +G
Sbjct:  370 DIDRSQGASRALSSFIDPSLSWKDIAFIKSITKMPIVIKG 409

[Close]

BLAST of EY734857 vs. ExPASy Swiss-Prot
Match: LLDD_ESCF3 (L-lactate dehydrogenase [cytochrome] OS=Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) GN=lldD PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 4.089e-17
Identity = 41/101 (40.59%), Postives = 61/101 (60.40%), Query Frame = 3

Query:  180 VKGVLTPQDARIA*SAEATGIIVSNHGPRQLDYVPATIMALEEVVKATHGRIPVLLDGGVKRGTDVFKTLAPEASDIFIGRPAAYSLTSAGHICLKTVLHM 482
            +KG+L P+DAR A    A GI+VSNHG RQLD V ++  AL  +  A  G I +L D G++ G DV + +A  A  + +GR   Y+L +AG   +  +L++
Sbjct:  250 IKGILDPEDARDAVRFGADGIVVSNHGGRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAYLYALATAGKAGVANLLNL 350

HSP 2 Score: 30.8018 bits (68), Expect = 4.089e-17
Identity = 16/50 (32.00%), Postives = 27/50 (54.00%), Query Frame = 2

Query:   53 DLGKMDE--ANDSGLAAYV---AGQIDRSLSWKDVKWLQTITKLPILSEG 187
            DLG +       +GL  Y+   A   D S+SWKD++W++     P++ +G
Sbjct:  203 DLGNISAYLGKPTGLEDYIGWLANNFDPSISWKDLEWIRDFWDGPMVIKG 252

The following BLAST results are available for this feature:

BLAST of EY734857 vs. ExPASy Swiss-Prot

BLAST of EY734857 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 125


Match Name	E-value	Identity	Description
LLDD_PSEAB	1.102e-17	39.60	L-lactate dehydrogenase [cytochrome] OS=Pseudomona...	[more]
LLDD_ALCFA	1.102e-17	37.40	L-lactate dehydrogenase [cytochrome] OS=Alcaligene...	[more]
LLDD_XANCP	2.421e-17	39.05	L-lactate dehydrogenase [cytochrome] OS=Xanthomona...	[more]
LLDD_XANCB	2.421e-17	39.05	L-lactate dehydrogenase [cytochrome] OS=Xanthomona...	[more]
LLDD_XANC8	2.421e-17	39.05	L-lactate dehydrogenase [cytochrome] OS=Xanthomona...	[more]
LLDD_ECOUT	3.145e-17	40.59	L-lactate dehydrogenase [cytochrome] OS=Escherichi...	[more]
LLDD_ECOK1	3.145e-17	40.59	L-lactate dehydrogenase [cytochrome] OS=Escherichi...	[more]
LLDD_ECO45	3.145e-17	40.59	L-lactate dehydrogenase [cytochrome] OS=Escherichi...	[more]
CYB2_HANAN	4.054e-17	39.58	Cytochrome b2, mitochondrial OS=Hansenula anomala ...	[more]
LLDD_ESCF3	4.089e-17	40.59	L-lactate dehydrogenase [cytochrome] OS=Escherichi...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-704-103-H12-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY734857 ID=EY734857; Name=EY734857; organism=Citrus sinensis; type=EST; length=820bp
CCACGCGTCCGCTCTACCACCATTTTTGACATTAAAGAATTTTCATGGTT
TGGACCTTGGCAAGATGGACGAGGCAAATGACTCTGGGCTTGCTGCCTAT
GTTGCTGGTCAAATTGATCGTTCTCTGAGCTGGAAGGACGTGAAGTGGCT
TCAAACAATCACCAAATTGCCAATTCTGAGTGAAGGGTGTACTCACACCA
CAGGATGCGCGGATTGCATGATCGGCTGAAGCAACTGGCATTATTGTATC
CAATCATGGACCTCGTCAACTTGACTATGTCCCTGCCACTATCATGGCCC
TGGAAGAGGTTGTGAAAGCTACTCATGGGCGTATTCCTGTGCTCTTGGAT
GGTGGTGTTAAGCGAGGAACTGATGTCTTCAAAACATTGGCACCTGAAGC
CTCTGATATATTTATTGGACGACCAGCGGCGTACTCATTGACATCTGCCG
GACACATATGCCTAAAAACAGTACTCCACATGCATAACAGAAAATACCAT
CTACCCACGGATCTAAATATGCCATCAATGATCCAACCTAAAACTGCCAT
ATCACCTAACCTTGTGTACGCGCCACCATCTCCTCTCCACCCCCTGTCTT
CACACTGCAGGATACCGACATCTACCAAACCACTCAATCTCAACAATTCC
ATTAACCCACTCTGTACTAACATATACCGTGCAAGGCATCAAACATCCAT
TACATATGCACGCACCTATATGCCACCCTCCCCCCACCTACTCCACACAT
CTACCTACCTCCCGGCCAACATACCAGATACCGCAACCAACGCTATATTG
CCGCTCATACCAGCCACTCA