EY732277

Overview
NameEY732277
Unique NameEY732277
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length851
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT_ZINEL (Caffeoyl-CoA O-methyltransferase OS=Zinnia elegans GN=CCOAOMT PE=2 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 2.768e-63
Identity = 70/92 (76.09%), Postives = 84/92 (91.30%), Query Frame = 2 
Query:  182 IIEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAPPDAKMDERIRCHRD 457
            II+KAG+AHKIDFREGPALPLLDQM+QDEK HG+FDFIFVDADK+NY+NYHKR+I+LVK GGVIGYDNTLW+GS+ AP DA + + +R +RD
Sbjct:  121 IIQKAGVAHKIDFREGPALPLLDQMLQDEKCHGSFDFIFVDADKDNYLNYHKRLIDLVKFGGVIGYDNTLWNGSLVAPADAPLRKYVRYYRD 212

HSP 2 Score: 84.7297 bits (208), Expect = 2.768e-63
Identity = 41/48 (85.42%), Postives = 46/48 (95.83%), Query Frame = 3 
Query:   27 LSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYYE 170
            L++LLKLINAKNTMEIGVYTGYSLL TALA+P+DGKILA+DINRE YE
Sbjct:   69 LNLLLKLINAKNTMEIGVYTGYSLLSTALALPEDGKILALDINRENYE 116

HSP 3 Score: 42.3578 bits (98), Expect = 2.768e-63
Identity = 18/27 (66.67%), Postives = 23/27 (85.19%), Query Frame = 3 
Query:  459 WVWELNRALPVDPPIEMCQLSVGDGVT 539
            +V ELN+AL VDP +E+CQL VGDG+T
Sbjct:  213 FVLELNKALAVDPRVEICQLPVGDGIT 239
[Close]
BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT2_TOBAC (Caffeoyl-CoA O-methyltransferase 2 OS=Nicotiana tabacum GN=CCOAOMT2 PE=2 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 3.605e-63
Identity = 70/92 (76.09%), Postives = 85/92 (92.39%), Query Frame = 2 
Query:  182 IIEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAPPDAKMDERIRCHRD 457
            IIEKAG+AHKI FREGPALP+LDQMI+D KYHG++DFIFVDADK+NY+NYHKR+I+LVKVGG+IGYDNTLW+GSV APPDA + + +R +RD
Sbjct:  118 IIEKAGLAHKIVFREGPALPVLDQMIEDGKYHGSYDFIFVDADKDNYLNYHKRLIDLVKVGGLIGYDNTLWNGSVVAPPDAPLRKYVRYYRD 209

HSP 2 Score: 88.1965 bits (217), Expect = 3.605e-63
Identity = 43/48 (89.58%), Postives = 46/48 (95.83%), Query Frame = 3 
Query:   27 LSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYYE 170
            LSMLLKLINAKNTMEIGV+TGYSLL TA+A+PDDGKILAMDINRE YE
Sbjct:   66 LSMLLKLINAKNTMEIGVFTGYSLLATAMALPDDGKILAMDINRENYE 113

HSP 3 Score: 38.1206 bits (87), Expect = 3.605e-63
Identity = 17/27 (62.96%), Postives = 21/27 (77.78%), Query Frame = 3 
Query:  459 WVWELNRALPVDPPIEMCQLSVGDGVT 539
            +V ELN+AL  D  IE+CQL VGDG+T
Sbjct:  210 FVLELNKALAADSRIEICQLPVGDGIT 236
[Close]
BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT3_TOBAC (Caffeoyl-CoA O-methyltransferase 3 OS=Nicotiana tabacum GN=CCOAOMT3 PE=2 SV=2)

HSP 1 Score: 156.762 bits (395), Expect = 6.111e-63
Identity = 68/92 (73.91%), Postives = 86/92 (93.48%), Query Frame = 2 
Query:  182 IIEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAPPDAKMDERIRCHRD 457
            +IEKAG+AHKI+F+EGPALP+LDQMI+D KYHG++DFIFVDADK+NY+NYHKR+I+LVKVGG+IGYDNTLW+GSV APPDA + + +R +RD
Sbjct:  118 VIEKAGLAHKIEFKEGPALPVLDQMIEDGKYHGSYDFIFVDADKDNYLNYHKRLIDLVKVGGLIGYDNTLWNGSVVAPPDAPLRKYVRYYRD 209

HSP 2 Score: 87.0409 bits (214), Expect = 6.111e-63
Identity = 42/48 (87.50%), Postives = 46/48 (95.83%), Query Frame = 3 
Query:   27 LSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYYE 170
            LSMLLKLINAKNTMEIGV+TGYSLL TA+A+PDDGKILAMDINR+ YE
Sbjct:   66 LSMLLKLINAKNTMEIGVFTGYSLLATAMALPDDGKILAMDINRDNYE 113

HSP 3 Score: 38.1206 bits (87), Expect = 6.111e-63
Identity = 17/27 (62.96%), Postives = 21/27 (77.78%), Query Frame = 3 
Query:  459 WVWELNRALPVDPPIEMCQLSVGDGVT 539
            +V ELN+AL  D  IE+CQL VGDG+T
Sbjct:  210 FVLELNKALAADSRIEICQLPVGDGIT 236
[Close]
BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT4_TOBAC (Caffeoyl-CoA O-methyltransferase 4 OS=Nicotiana tabacum GN=CCOAOMT4 PE=2 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 6.111e-63
Identity = 67/92 (72.83%), Postives = 86/92 (93.48%), Query Frame = 2 
Query:  182 IIEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAPPDAKMDERIRCHRD 457
            +IEKAG+AHKI+F+EGPALP+LDQMI+D KYHG++DFIFVDADK+NY+NYHKR+I+LVK+GG+IGYDNTLW+GSV APPDA + + +R +RD
Sbjct:  118 VIEKAGLAHKIEFKEGPALPVLDQMIEDGKYHGSYDFIFVDADKDNYLNYHKRLIDLVKIGGLIGYDNTLWNGSVVAPPDAPLRKYVRYYRD 209

HSP 2 Score: 87.4261 bits (215), Expect = 6.111e-63
Identity = 42/48 (87.50%), Postives = 46/48 (95.83%), Query Frame = 3 
Query:   27 LSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYYE 170
            LSML+KLINAKNTMEIGV+TGYSLL TA+A+PDDGKILAMDINRE YE
Sbjct:   66 LSMLIKLINAKNTMEIGVFTGYSLLATAMALPDDGKILAMDINRENYE 113

HSP 3 Score: 38.1206 bits (87), Expect = 6.111e-63
Identity = 17/27 (62.96%), Postives = 21/27 (77.78%), Query Frame = 3 
Query:  459 WVWELNRALPVDPPIEMCQLSVGDGVT 539
            +V ELN+AL  D  IE+CQL VGDG+T
Sbjct:  210 FVLELNKALAADSRIEICQLPVGDGIT 236
[Close]
BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT1_TOBAC (Caffeoyl-CoA O-methyltransferase 1 OS=Nicotiana tabacum GN=CCOAOMT1 PE=1 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 6.112e-63
Identity = 67/92 (72.83%), Postives = 86/92 (93.48%), Query Frame = 2 
Query:  182 IIEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAPPDAKMDERIRCHRD 457
            +IEKAG+AHKI+F+EGPALP+LDQMI+D KYHG++DFIFVDADK+NY+NYHKR+I+LVK+GG+IGYDNTLW+GSV APPDA + + +R +RD
Sbjct:  115 VIEKAGLAHKIEFKEGPALPVLDQMIEDGKYHGSYDFIFVDADKDNYLNYHKRLIDLVKIGGLIGYDNTLWNGSVVAPPDAPLRKYVRYYRD 206

HSP 2 Score: 87.4261 bits (215), Expect = 6.112e-63
Identity = 42/48 (87.50%), Postives = 46/48 (95.83%), Query Frame = 3 
Query:   27 LSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYYE 170
            LSML+KLINAKNTMEIGV+TGYSLL TA+A+PDDGKILAMDINRE YE
Sbjct:   63 LSMLIKLINAKNTMEIGVFTGYSLLATAMALPDDGKILAMDINRENYE 110

HSP 3 Score: 38.1206 bits (87), Expect = 6.112e-63
Identity = 17/27 (62.96%), Postives = 21/27 (77.78%), Query Frame = 3 
Query:  459 WVWELNRALPVDPPIEMCQLSVGDGVT 539
            +V ELN+AL  D  IE+CQL VGDG+T
Sbjct:  207 FVLELNKALAADSRIEICQLPVGDGIT 233
[Close]
BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT2_POPTR (Caffeoyl-CoA O-methyltransferase 2 OS=Populus trichocarpa GN=CCOAOMT2 PE=2 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 7.953e-63
Identity = 69/92 (75.00%), Postives = 84/92 (91.30%), Query Frame = 2 
Query:  182 IIEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAPPDAKMDERIRCHRD 457
            +I+KAG+ HKI+F+EGPALP+LDQMI+D KYHGT+DFIFVDADK+NYINYHKR+IELVKVGG+IGYDNTLW+GSV AP DA M + +R +RD
Sbjct:  123 VIQKAGLEHKIEFKEGPALPVLDQMIEDGKYHGTYDFIFVDADKDNYINYHKRLIELVKVGGLIGYDNTLWNGSVVAPADAPMRKYVRYYRD 214

HSP 2 Score: 87.0409 bits (214), Expect = 7.953e-63
Identity = 43/48 (89.58%), Postives = 46/48 (95.83%), Query Frame = 3 
Query:   27 LSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYYE 170
            L+MLLKLINAKNTMEIGV+TGYSLL TALAIP+DGKILAMDINRE YE
Sbjct:   71 LNMLLKLINAKNTMEIGVFTGYSLLATALAIPEDGKILAMDINRENYE 118

HSP 3 Score: 39.2762 bits (90), Expect = 7.953e-63
Identity = 17/27 (62.96%), Postives = 21/27 (77.78%), Query Frame = 3 
Query:  459 WVWELNRALPVDPPIEMCQLSVGDGVT 539
            +V ELN+AL  DP IE+C L VGDG+T
Sbjct:  215 FVLELNKALAADPRIEICMLPVGDGIT 241
[Close]
BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT_MESCR (Caffeoyl-CoA O-methyltransferase OS=Mesembryanthemum crystallinum PE=2 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 1.195e-60
Identity = 67/92 (72.83%), Postives = 82/92 (89.13%), Query Frame = 2 
Query:  182 IIEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAPPDAKMDERIRCHRD 457
            +I+KAG+AHKIDFREGPALP+LD +I+D K HG+FDF FVDADK+NY NYHKR+I+LVK+GGVIGYDNTLW+GSVAAP DA M + +R +RD
Sbjct:  130 VIQKAGVAHKIDFREGPALPVLDLLIEDAKNHGSFDFAFVDADKDNYGNYHKRLIDLVKIGGVIGYDNTLWNGSVAAPADAPMRKYVRYYRD 221

HSP 2 Score: 86.2705 bits (212), Expect = 1.195e-60
Identity = 42/48 (87.50%), Postives = 46/48 (95.83%), Query Frame = 3 
Query:   27 LSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYYE 170
            L+MLLKLINAKNT+EIGVYTGYSLL +ALA+PDDGKILAMDINRE YE
Sbjct:   78 LNMLLKLINAKNTIEIGVYTGYSLLASALALPDDGKILAMDINRENYE 125

HSP 3 Score: 38.891 bits (89), Expect = 1.195e-60
Identity = 15/27 (55.56%), Postives = 21/27 (77.78%), Query Frame = 3 
Query:  459 WVWELNRALPVDPPIEMCQLSVGDGVT 539
            +V E N+A+  DP +E+CQL VGDG+T
Sbjct:  222 FVMEFNKAIAADPRVEICQLPVGDGIT 248
[Close]
BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT_CITNA (Caffeoyl-CoA O-methyltransferase OS=Citrus natsudaidai PE=3 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 1.196e-60
Identity = 68/91 (74.73%), Postives = 82/91 (90.11%), Query Frame = 2 
Query:  185 IEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAPPDAKMDERIRCHRD 457
            IEKAG+AH+I FREGPALP+LDQ+++D+K HGT+DFIFVDADK+NYINYHKRII+LVKVGG+IGYDNTLW+GSV  PPDA M + +R  RD
Sbjct:  109 IEKAGVAHRIQFREGPALPVLDQLVEDKKNHGTYDFIFVDADKDNYINYHKRIIDLVKVGGLIGYDNTLWNGSVVGPPDAPMRKYLRYCRD 199

HSP 2 Score: 85.1149 bits (209), Expect = 1.196e-60
Identity = 43/48 (89.58%), Postives = 44/48 (91.67%), Query Frame = 3 
Query:   27 LSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYYE 170
            L+M LKLINAKNTMEIGVYT YSLL TALAIPDDGKILAMDINRE YE
Sbjct:   56 LNMSLKLINAKNTMEIGVYTDYSLLATALAIPDDGKILAMDINRENYE 103

HSP 3 Score: 37.3502 bits (85), Expect = 1.196e-60
Identity = 17/27 (62.96%), Postives = 21/27 (77.78%), Query Frame = 3 
Query:  459 WVWELNRALPVDPPIEMCQLSVGDGVT 539
            +V EL +AL VDP IE+C L VGDG+T
Sbjct:  200 FVLELIKALAVDPRIEICMLPVGDGIT 226
[Close]
BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT_EUCGU (Caffeoyl-CoA O-methyltransferase OS=Eucalyptus gunnii PE=2 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 2.025e-60
Identity = 67/92 (72.83%), Postives = 83/92 (90.22%), Query Frame = 2 
Query:  182 IIEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAPPDAKMDERIRCHRD 457
            +I+KAG+A KIDFREGPALP+LDQ+I+D K  G+FDFIFVDADK+NY+NYHKR+IELVKVGG+IGYDNTLW+GSV APPDA + + +R +RD
Sbjct:  123 VIQKAGVADKIDFREGPALPILDQLIEDGK-QGSFDFIFVDADKDNYLNYHKRLIELVKVGGLIGYDNTLWNGSVVAPPDAPLRKYVRYYRD 213

HSP 2 Score: 87.8113 bits (216), Expect = 2.025e-60
Identity = 43/48 (89.58%), Postives = 46/48 (95.83%), Query Frame = 3 
Query:   27 LSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYYE 170
            L+MLLKLINAKNTMEIGV+TGYSLL TALA+PDDGKILAMDINRE YE
Sbjct:   71 LNMLLKLINAKNTMEIGVFTGYSLLATALALPDDGKILAMDINRENYE 118

HSP 3 Score: 39.2762 bits (90), Expect = 2.025e-60
Identity = 17/27 (62.96%), Postives = 21/27 (77.78%), Query Frame = 3 
Query:  459 WVWELNRALPVDPPIEMCQLSVGDGVT 539
            +V ELN+AL  DP IE+C L VGDG+T
Sbjct:  214 FVLELNKALAADPRIEICMLPVGDGIT 240
[Close]
BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT1_EUCGL (Caffeoyl-CoA O-methyltransferase 1 OS=Eucalyptus globulus GN=CCOMT PE=2 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 2.025e-60
Identity = 67/92 (72.83%), Postives = 83/92 (90.22%), Query Frame = 2 
Query:  182 IIEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAPPDAKMDERIRCHRD 457
            +I+KAG+A KIDFREGPALP+LDQ+I+D K  G+FDFIFVDADK+NY+NYHKR+IELVKVGG+IGYDNTLW+GSV APPDA + + +R +RD
Sbjct:  123 VIQKAGVADKIDFREGPALPILDQLIEDGK-QGSFDFIFVDADKDNYLNYHKRLIELVKVGGLIGYDNTLWNGSVVAPPDAPLRKYVRYYRD 213

HSP 2 Score: 87.8113 bits (216), Expect = 2.025e-60
Identity = 43/48 (89.58%), Postives = 46/48 (95.83%), Query Frame = 3 
Query:   27 LSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYYE 170
            L+MLLKLINAKNTMEIGV+TGYSLL TALA+PDDGKILAMDINRE YE
Sbjct:   71 LNMLLKLINAKNTMEIGVFTGYSLLATALALPDDGKILAMDINRENYE 118

HSP 3 Score: 39.2762 bits (90), Expect = 2.025e-60
Identity = 17/27 (62.96%), Postives = 21/27 (77.78%), Query Frame = 3 
Query:  459 WVWELNRALPVDPPIEMCQLSVGDGVT 539
            +V ELN+AL  DP IE+C L VGDG+T
Sbjct:  214 FVLELNKALAADPRIEICMLPVGDGIT 240
The following BLAST results are available for this feature:
BLAST of EY732277 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 33
Match NameE-valueIdentityDescription
CAMT_ZINEL2.768e-6376.09Caffeoyl-CoA O-methyltransferase OS=Zinnia elegans... [more]
CAMT2_TOBAC3.605e-6376.09Caffeoyl-CoA O-methyltransferase 2 OS=Nicotiana ta... [more]
CAMT3_TOBAC6.111e-6373.91Caffeoyl-CoA O-methyltransferase 3 OS=Nicotiana ta... [more]
CAMT4_TOBAC6.111e-6372.83Caffeoyl-CoA O-methyltransferase 4 OS=Nicotiana ta... [more]
CAMT1_TOBAC6.112e-6372.83Caffeoyl-CoA O-methyltransferase 1 OS=Nicotiana ta... [more]
CAMT2_POPTR7.953e-6375.00Caffeoyl-CoA O-methyltransferase 2 OS=Populus tric... [more]
CAMT_MESCR1.195e-6072.83Caffeoyl-CoA O-methyltransferase OS=Mesembryanthem... [more]
CAMT_CITNA1.196e-6074.73Caffeoyl-CoA O-methyltransferase OS=Citrus natsuda... [more]
CAMT_EUCGU2.025e-6072.83Caffeoyl-CoA O-methyltransferase OS=Eucalyptus gun... [more]
CAMT1_EUCGL2.025e-6072.83Caffeoyl-CoA O-methyltransferase 1 OS=Eucalyptus g... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-059-E02-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY732277 ID=EY732277; Name=EY732277; organism=Citrus sinensis; type=EST; length=851bp
CACGCGTCCGCTGACGAAGCACAATCCTGAGCATGCTACTCAAGCTTATC
AACGCCAAGAATACCATGGAAATTGGTGTTTATACTGGTTACTCTCTCCT
CGTAACTGCCCTTGCTATTCCCGACGACGGCAAGATATTGGCCATGGACA
TCAATAGAGAATACTACGAACTCGGTCTCTCATAATTGAAAAGGCTGGTA
TTGCTCACAAGATTGACTTCAGAGAGGGCCCAGCTCTCCCTCTTCTCGAT
CAAATGATCCAAGACGAAAAATATCATGGGACATTTGACTTTATCTTTGT
TGATGCTGATAAGAATAACTACATTAACTACCACAAGAGGATTATTGAAC
TTGTGAAGGTTGGGGGAGTGATTGGCTACGACAACACGCTGTGGTCTGGA
TCTGTAGCTGCACCACCTGACGCAAAAATGGATGAGAGAATCAGATGTCA
CAGGGACTTGGGTGTGGGAATTGAACCGGGCTCTCCCTGTTGATCCACCG
ATCGAGATGTGCCAACTCTCTGTAGGTGATGGGGTGACATTGTGTCGCCG
CCTCCTTCGAGCATTATGTATCCGTGGATCCCCACGATAGCGGCACGCGT
TGCAGGATACCACTAGGGGGGGTGGGGAACAAATGAAAGCGCGTGGCGCG
GAGCTTTCAGAGGCGCGGGGGAGTGAGAAGGGGGGAGTTACGAACCCTGT
GGGGGTCGGGGGTCGCGGGCGCGGGCGGGGGGGCAGGGCTGGAGCGGGGG
AGGACCGCGAGGGCCGCGCCCCCGCGTCCCCCACGCTTGTCCCCGTCCCC
ACTGCGCGCGCCGCCGCGTGGAGTATAGATGTTTCTTGGTGGCTGTGTGC
G
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