EY732277 | Citrus Genome Database

Overview


Name	EY732277
Unique Name	EY732277
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	851

Libraries


Library Name	Type
Sweet orange fruit, development stadium (5 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT_PINTA (Caffeoyl-CoA O-methyltransferase OS=Pinus taeda GN=CCOAOMT PE=2 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 2.828e-59
Identity = 64/92 (69.57%), Postives = 83/92 (90.22%), Query Frame = 2

Query:  182 IIEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAPPDAKMDERIRCHRD 457
            IIEKAG+AHKIDFREGPALP+LD+++++E  HG+FDF+FVD DK+NY+NYHKR+I+LVKVGG+I YDNTLW+GSV APPDA + + +R +RD
Sbjct:  135 IIEKAGVAHKIDFREGPALPVLDELLKNEDMHGSFDFVFVDRDKDNYLNYHKRLIDLVKVGGLIAYDNTLWNGSVVAPPDAPLRKYVRYYRD 226

HSP 2 Score: 85.8853 bits (211), Expect = 2.828e-59
Identity = 42/48 (87.50%), Postives = 45/48 (93.75%), Query Frame = 3

Query:   27 LSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYYE 170
            L +LLKLINAKNTMEIGVYTGYSLL TALA+PDDGKILAMDINRE Y+
Sbjct:   83 LGLLLKLINAKNTMEIGVYTGYSLLSTALALPDDGKILAMDINRENYD 130

HSP 3 Score: 35.039 bits (79), Expect = 2.828e-59
Identity = 17/27 (62.96%), Postives = 21/27 (77.78%), Query Frame = 3

Query:  459 WVWELNRALPVDPPIEMCQLSVGDGVT 539
            +V ELN+AL VDP IE+ Q+ V DGVT
Sbjct:  227 FVMELNKALAVDPRIEISQIPVLDGVT 253

[Close]

BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT1_MAIZE (Caffeoyl-CoA O-methyltransferase 1 OS=Zea mays GN=CCOAOMT1 PE=2 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 9.343e-57
Identity = 64/91 (70.33%), Postives = 81/91 (89.01%), Query Frame = 2

Query:  185 IEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAPPDAKMDERIRCHRD 457
            IEKAG+AHKIDFREGPALP+LD +I +EK HG+FDF+FVDADK+NY+NYH+R+++LVK+GG+IGYDNTLW+GSV  P DA M + IR +RD
Sbjct:  135 IEKAGVAHKIDFREGPALPVLDDLIAEEKNHGSFDFVFVDADKDNYLNYHERLLKLVKLGGLIGYDNTLWNGSVVLPDDAPMRKYIRFYRD 225

HSP 2 Score: 80.1073 bits (196), Expect = 9.343e-57
Identity = 39/48 (81.25%), Postives = 43/48 (89.58%), Query Frame = 3

Query:   27 LSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYYE 170
            L+ML+KLI AK TMEIGVYTGYSLL TALA+P+DG ILAMDINRE YE
Sbjct:   82 LNMLIKLIGAKKTMEIGVYTGYSLLATALALPEDGTILAMDINRENYE 129

HSP 3 Score: 35.4242 bits (80), Expect = 9.343e-57
Identity = 16/27 (59.26%), Postives = 20/27 (74.07%), Query Frame = 3

Query:  459 WVWELNRALPVDPPIEMCQLSVGDGVT 539
            +V  LN+AL  D  +E+CQL VGDGVT
Sbjct:  226 FVLVLNKALAADDRVEICQLPVGDGVT 252

[Close]

BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT2_MAIZE (Caffeoyl-CoA O-methyltransferase 2 OS=Zea mays GN=CCOAOMT2 PE=2 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 6.780e-54
Identity = 59/91 (64.84%), Postives = 77/91 (84.62%), Query Frame = 2

Query:  185 IEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAPPDAKMDERIRCHRD 457
            I KAG+ HKIDFREGPALP+LD ++ D++ HG+FDF FVDADK+NY+NYH+R+++LV+ GG+IGYDNTLW+GSV  P DA M + IR +RD
Sbjct:  141 INKAGVGHKIDFREGPALPVLDDLVADKEQHGSFDFAFVDADKDNYLNYHERLLKLVRPGGLIGYDNTLWNGSVVLPDDAPMRKYIRFYRD 231

HSP 2 Score: 80.1073 bits (196), Expect = 6.780e-54
Identity = 39/48 (81.25%), Postives = 43/48 (89.58%), Query Frame = 3

Query:   27 LSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYYE 170
            L+ML+KLI AK TMEIGVYTGYSLL TALA+P+DG ILAMDINRE YE
Sbjct:   88 LNMLIKLIGAKKTMEIGVYTGYSLLATALALPEDGTILAMDINRENYE 135

HSP 3 Score: 34.6538 bits (78), Expect = 6.780e-54
Identity = 16/27 (59.26%), Postives = 19/27 (70.37%), Query Frame = 3

Query:  459 WVWELNRALPVDPPIEMCQLSVGDGVT 539
            +V  LN AL  D  +E+CQL VGDGVT
Sbjct:  232 FVLALNSALAADDRVEICQLPVGDGVT 258

[Close]

BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT3_ARATH (Probable caffeoyl-CoA O-methyltransferase At4g26220 OS=Arabidopsis thaliana GN=At4g26220 PE=2 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 3.973e-42
Identity = 51/87 (58.62%), Postives = 66/87 (75.86%), Query Frame = 2

Query:  182 IIEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAPPDAKMDERI 442
            +I+KAG+ HKIDF+E  ALP LD+++ ++   G FDF FVDADK NY NYH+R+I L+KVGG+I YDNTLW GSVA P  +  + RI
Sbjct:  109 VIKKAGVEHKIDFKESEALPALDELLNNKVNEGGFDFAFVDADKLNYWNYHERLIRLIKVGGIIVYDNTLWGGSVAEPDSSTPEWRI 195

HSP 2 Score: 72.0182 bits (175), Expect = 3.973e-42
Identity = 29/49 (59.18%), Postives = 44/49 (89.80%), Query Frame = 3

Query:   24 ILSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYYE 170
            ++ MLL L+NA+ T+E+GV+TGYSLL+TAL +P+DGK++A+D+NR+ YE
Sbjct:   56 LMGMLLNLVNARKTIEVGVFTGYSLLLTALTLPEDGKVIAIDMNRDSYE 104

HSP 3 Score: 27.335 bits (59), Expect = 3.973e-42
Identity = 10/24 (41.67%), Postives = 17/24 (70.83%), Query Frame = 3

Query:  468 ELNRALPVDPPIEMCQLSVGDGVT 539
            ELN+ L  D  +++ Q ++GDG+T
Sbjct:  203 ELNKKLSADQRVQISQAALGDGIT 226

[Close]

BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT_STELP (Caffeoyl-CoA O-methyltransferase OS=Stellaria longipes PE=2 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.162e-39
Identity = 47/78 (60.26%), Postives = 61/78 (78.21%), Query Frame = 2

Query:  182 IIEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAP 415
            +I+KAG+  KI F    A+ LLD ++ D +Y G++DF FVDADK NY+NYH+R+IELVKVGG+I YDNTLW G+VA P
Sbjct:  118 LIKKAGVESKISFIVSDAMTLLDDLLADGRYQGSYDFAFVDADKTNYVNYHERLIELVKVGGIIAYDNTLWGGTVALP 195

HSP 2 Score: 68.5514 bits (166), Expect = 2.162e-39
Identity = 31/48 (64.58%), Postives = 40/48 (83.33%), Query Frame = 3

Query:   24 ILSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYY 167
            +LS +LK +  K T+E+GV+TGYSLL TAL+IPDDGKI A+DI+RE Y
Sbjct:   65 LLSFMLKTVKPKKTIEVGVFTGYSLLATALSIPDDGKITAVDIDREAY 112

HSP 3 Score: 25.7942 bits (55), Expect = 2.162e-39
Identity = 12/26 (46.15%), Postives = 16/26 (61.54%), Query Frame = 3

Query:  462 VWELNRALPVDPPIEMCQLSVGDGVT 539
            V +LN  L  D  I++  L VGDG+T
Sbjct:  210 VTKLNEILGSDARIDIAHLPVGDGIT 235

[Close]

BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT1_ARATH (Putative caffeoyl-CoA O-methyltransferase At1g67980 OS=Arabidopsis thaliana GN=At1g67980 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 2.982e-38
Identity = 44/90 (48.89%), Postives = 63/90 (70.00%), Query Frame = 2

Query:  185 IEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAPPDAKMDERIRCHR 454
            I+KAG+ HKI+F     L  LDQ++ D+     FDF F DADK++Y+N+H+R+++LVKVGG+I +DNTLW G VA   D  + E +R +R
Sbjct:  113 IKKAGVDHKINFIHSDGLKALDQLVNDKC---EFDFAFADADKSSYVNFHERLLKLVKVGGIIAFDNTLWFGFVAEDEDG-VPEHMREYR 198

HSP 2 Score: 75.0998 bits (183), Expect = 2.982e-38
Identity = 34/48 (70.83%), Postives = 45/48 (93.75%), Query Frame = 3

Query:   27 LSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYYE 170
            LSML+K++NAKNT+EIGV+TGYSLL TALA+P+DG+I A+DI++E YE
Sbjct:   60 LSMLVKIMNAKNTIEIGVFTGYSLLTTALALPEDGRITAIDIDKEAYE 107

HSP 3 Score: 33.4982 bits (75), Expect = 2.982e-38
Identity = 12/24 (50.00%), Postives = 19/24 (79.17%), Query Frame = 3

Query:  468 ELNRALPVDPPIEMCQLSVGDGVT 539
            E N+ L +DP +E+ Q+S+GDG+T
Sbjct:  203 EFNKKLALDPRVEVSQISIGDGIT 226

[Close]

BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT_POPKI (Caffeoyl-CoA O-methyltransferase OS=Populus kitakamiensis PE=2 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.238e-35
Identity = 43/90 (47.78%), Postives = 66/90 (73.33%), Query Frame = 2

Query:  185 IEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAPPDAKMDERIRCHR 454
            I+KAG+  KI+F +  A P+L++M+ ++K    FDF FVDADK++Y +YH+++++LVK+GG+I YDNTLW G VA   D ++ E +R  R
Sbjct:  113 IQKAGVEDKINFIQSEAPPILNEMLCNDK-QPEFDFAFVDADKSSYKHYHEQLLKLVKIGGIIAYDNTLWYGLVAKEVDDEVPEPLRMVR 201

HSP 2 Score: 68.9366 bits (167), Expect = 1.238e-35
Identity = 32/48 (66.67%), Postives = 42/48 (87.50%), Query Frame = 3

Query:   27 LSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYYE 170
            LSMLLKL+NAK T+E+GV+TGYSLL TALA+P+DG++ A+D +R  YE
Sbjct:   60 LSMLLKLMNAKRTLEVGVFTGYSLLSTALALPEDGQVTAIDKDRGAYE 107

HSP 3 Score: 30.4166 bits (67), Expect = 1.238e-35
Identity = 13/28 (46.43%), Postives = 19/28 (67.86%), Query Frame = 3

Query:  456 TWVWELNRALPVDPPIEMCQLSVGDGVT 539
            T + E N+ L  D  +E+ Q+S+GDGVT
Sbjct:  202 TVIMEFNKLLSSDLRVEISQISIGDGVT 229

[Close]

BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT2_ARATH (Probable caffeoyl-CoA O-methyltransferase At1g67990 OS=Arabidopsis thaliana GN=At1g67990 PE=2 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 5.110e-33
Identity = 36/74 (48.65%), Postives = 56/74 (75.68%), Query Frame = 2

Query:  185 IEKAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSV 406
            ++KAG+ HKI+F +  A+  LDQ++  EK    +DF FVDADK NY+ + +++++LVKVGG+I +DNTLW G++
Sbjct:  113 MKKAGVDHKINFIQSDAVRGLDQLLNGEKQE--YDFAFVDADKTNYVYFLEKLLKLVKVGGIIAFDNTLWFGTL 184

HSP 2 Score: 68.9366 bits (167), Expect = 5.110e-33
Identity = 32/50 (64.00%), Postives = 42/50 (84.00%), Query Frame = 3

Query:   18 STILSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREYY 167
            S  LSML+K+INAKNT+EIGV+TGYSL   ALA+P+DG+I A+DI++  Y
Sbjct:   57 SLFLSMLVKIINAKNTIEIGVFTGYSLFTVALALPEDGRITAIDIDQAGY 106

HSP 3 Score: 31.5722 bits (70), Expect = 5.110e-33
Identity = 12/24 (50.00%), Postives = 18/24 (75.00%), Query Frame = 3

Query:  468 ELNRALPVDPPIEMCQLSVGDGVT 539
            E N+ L  DP +E+ Q+S+GDG+T
Sbjct:  204 EFNKILARDPRVEIAQISIGDGLT 227

[Close]

BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT2_DICDI (Probable caffeoyl-CoA O-methyltransferase 2 OS=Dictyostelium discoideum GN=omt6 PE=1 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 5.644e-21
Identity = 32/75 (42.67%), Postives = 48/75 (64.00%), Query Frame = 2

Query:  191 KAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAP 415
            KAG+ HKI+ +  PA   L ++I D+    T+DF+F+DADK  Y  Y++  ++L++ GG+I  DN L  G VA P
Sbjct:  116 KAGVDHKINLKIQPASKTLQELI-DQGEENTYDFVFIDADKTGYDTYYELSLKLIRKGGIIAIDNVLQHGRVADP 189

HSP 2 Score: 52.373 bits (124), Expect = 5.644e-21
Identity = 21/48 (43.75%), Postives = 37/48 (77.08%), Query Frame = 3

Query:   21 TILSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREY 164
            +   +L++++NAK T++IGV+TG S L  ALA+ D+G+++A D++ EY
Sbjct:   59 SFFKLLIQVLNAKKTIDIGVFTGLSSLTAALAMGDEGRVVACDVSTEY 106

[Close]

BLAST of EY732277 vs. ExPASy Swiss-Prot
Match: CAMT1_DICDI (Probable caffeoyl-CoA O-methyltransferase 1 OS=Dictyostelium discoideum GN=omt5 PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.243e-20
Identity = 32/75 (42.67%), Postives = 48/75 (64.00%), Query Frame = 2

Query:  191 KAGIAHKIDFREGPALPLLDQMIQDEKYHGTFDFIFVDADKNNYINYHKRIIELVKVGGVIGYDNTLWSGSVAAP 415
            KAG+ HKI+ +  PA   L ++I D+    T+DF+F+DADK  Y  Y++  ++L++ GG+I  DN L  G VA P
Sbjct:  115 KAGVDHKINLKIQPASKTLQELI-DQGEENTYDFVFIDADKTGYDTYYELSLKLIRKGGIIAIDNVLQHGRVADP 188

HSP 2 Score: 51.2174 bits (121), Expect = 1.243e-20
Identity = 20/48 (41.67%), Postives = 37/48 (77.08%), Query Frame = 3

Query:   21 TILSMLLKLINAKNTMEIGVYTGYSLLVTALAIPDDGKILAMDINREY 164
            +   +L++++NAK T++IGV+TG S L  ALA+ D+G+++A D++ +Y
Sbjct:   58 SFFKLLIQVLNAKKTIDIGVFTGLSSLTAALAMGDEGRVVACDVSTDY 105

The following BLAST results are available for this feature:

BLAST of EY732277 vs. ExPASy Swiss-Prot

BLAST of EY732277 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 33


Match Name	E-value	Identity	Description
CAMT_PINTA	2.828e-59	69.57	Caffeoyl-CoA O-methyltransferase OS=Pinus taeda GN...	[more]
CAMT1_MAIZE	9.343e-57	70.33	Caffeoyl-CoA O-methyltransferase 1 OS=Zea mays GN=...	[more]
CAMT2_MAIZE	6.780e-54	64.84	Caffeoyl-CoA O-methyltransferase 2 OS=Zea mays GN=...	[more]
CAMT3_ARATH	3.973e-42	58.62	Probable caffeoyl-CoA O-methyltransferase At4g2622...	[more]
CAMT_STELP	2.162e-39	60.26	Caffeoyl-CoA O-methyltransferase OS=Stellaria long...	[more]
CAMT1_ARATH	2.982e-38	48.89	Putative caffeoyl-CoA O-methyltransferase At1g6798...	[more]
CAMT_POPKI	1.238e-35	47.78	Caffeoyl-CoA O-methyltransferase OS=Populus kitaka...	[more]
CAMT2_ARATH	5.110e-33	48.65	Probable caffeoyl-CoA O-methyltransferase At1g6799...	[more]
CAMT2_DICDI	5.644e-21	42.67	Probable caffeoyl-CoA O-methyltransferase 2 OS=Dic...	[more]
CAMT1_DICDI	1.243e-20	42.67	Probable caffeoyl-CoA O-methyltransferase 1 OS=Dic...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-704-059-E02-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY732277 ID=EY732277; Name=EY732277; organism=Citrus sinensis; type=EST; length=851bp
CACGCGTCCGCTGACGAAGCACAATCCTGAGCATGCTACTCAAGCTTATC
AACGCCAAGAATACCATGGAAATTGGTGTTTATACTGGTTACTCTCTCCT
CGTAACTGCCCTTGCTATTCCCGACGACGGCAAGATATTGGCCATGGACA
TCAATAGAGAATACTACGAACTCGGTCTCTCATAATTGAAAAGGCTGGTA
TTGCTCACAAGATTGACTTCAGAGAGGGCCCAGCTCTCCCTCTTCTCGAT
CAAATGATCCAAGACGAAAAATATCATGGGACATTTGACTTTATCTTTGT
TGATGCTGATAAGAATAACTACATTAACTACCACAAGAGGATTATTGAAC
TTGTGAAGGTTGGGGGAGTGATTGGCTACGACAACACGCTGTGGTCTGGA
TCTGTAGCTGCACCACCTGACGCAAAAATGGATGAGAGAATCAGATGTCA
CAGGGACTTGGGTGTGGGAATTGAACCGGGCTCTCCCTGTTGATCCACCG
ATCGAGATGTGCCAACTCTCTGTAGGTGATGGGGTGACATTGTGTCGCCG
CCTCCTTCGAGCATTATGTATCCGTGGATCCCCACGATAGCGGCACGCGT
TGCAGGATACCACTAGGGGGGGTGGGGAACAAATGAAAGCGCGTGGCGCG
GAGCTTTCAGAGGCGCGGGGGAGTGAGAAGGGGGGAGTTACGAACCCTGT
GGGGGTCGGGGGTCGCGGGCGCGGGCGGGGGGGCAGGGCTGGAGCGGGGG
AGGACCGCGAGGGCCGCGCCCCCGCGTCCCCCACGCTTGTCCCCGTCCCC
ACTGCGCGCGCCGCCGCGTGGAGTATAGATGTTTCTTGGTGGCTGTGTGC
G