EY756300

Overview
NameEY756300
Unique NameEY756300
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length899
Libraries
Library NameType
Sweet orange leaf, field plant Bcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY756300 vs. ExPASy Swiss-Prot
Match: AQP1_SHEEP (Aquaporin-1 OS=Ovis aries GN=AQP1 PE=2 SV=3)

HSP 1 Score: 86.2705 bits (212), Expect = 5.759e-18
Identity = 58/172 (33.72%), Postives = 87/172 (50.58%), Query Frame = 3 
Query:  132 FKAYLAEFISTLLFVFAGVGSAIAFN-----KMTADAALDPSGLVAIAICHGFALFVAVAIGANISGGHVNPAVTFGLALGGQITILTGIFYWIAQLLGSIVASFLLKVVTGGL---AVPTHNXXXXXXXXXXXXXXXXXTFGLVYTVYATAADPKKGSLGTIAPIAIGFIV 623
            ++A +AEF++ +LF+F  +GSA+ F+       T  A  D    V +++  G ++        +ISG H+NPAVT GL L  QI+IL  I Y IAQ +G+IVA+ +L  +T  L   ++  +                  T  LV  V AT    ++  LG   P+AIGF V
Sbjct:   11 WRAVVAEFLAMILFIFISIGSALGFHYPIKSNQTTGAVQDN---VKVSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISILRAIMYIIAQCVGAIVATVILSGITSSLPDNSLGLNALAPGVNSGQGLGIEIIGTLQLVLCVLATTDRRRRRDLGDSGPLAIGFSV 179

HSP 2 Score: 24.6386 bits (52), Expect = 5.759e-18
Identity = 10/18 (55.56%), Postives = 13/18 (72.22%), Query Frame = 2 
Query:  665 MNPXPSFGPAVASGNFQD 718
            +NP  SFG +V + NFQD
Sbjct:  194 INPARSFGSSVITHNFQD 211

HSP 3 Score: 20.4014 bits (41), Expect = 5.759e-18
Identity = 5/6 (83.33%), Postives = 6/6 (100.00%), Query Frame = 1 
Query:  718 HWIYWV 735
            HWI+WV
Sbjct:  212 HWIFWV 217
[Close]
BLAST of EY756300 vs. ExPASy Swiss-Prot
Match: MIP_BOVIN (Lens fiber major intrinsic protein OS=Bos taurus GN=MIP PE=1 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.769e-18
Identity = 58/186 (31.18%), Postives = 92/186 (49.46%), Query Frame = 3 
Query:  120 SLGSFKAYLAEFISTLLFVFAGVGSAIAFNKMTADAALDPSGLVAIAICHGFALFVAVAIGANISGGHVNPAVTFGLALGGQITILTGIFYWIAQLLGSIVASFLLKVVT-----GGLAVPTHNXXXXXXXXXXXXXXXXXTFGLVYTVYATAADPKKGSLGTIA-----PIAIGFIVGANIPGAG 647
            S   ++A  AEF ++L +VF G+G+++ +       A  P  ++ +A+  G AL   V    +ISG HVNPAVTF   +G Q+++L  I Y +AQLLG++  + +L  VT     G LA+ T                   T   V  ++AT  + + G LG++A      + +G + G    GAG
Sbjct:    6 SASFWRAICAEFFASLFYVFFGLGASLRW-------APGPLHVLQVALAFGLALATLVQAVGHISGAHVNPAVTFAFLVGSQMSLLRAICYMVAQLLGAVAGAAVLYSVTPPAVRGNLALNT--LHPGVSVGQATIVEIFLTLQFVLCIFATYDERRNGRLGSVALAVGFSLTLGHLFGMYYTGAG 182

HSP 2 Score: 23.8682 bits (50), Expect = 5.769e-18
Identity = 6/11 (54.55%), Postives = 8/11 (72.73%), Query Frame = 1 
Query:  703 RQLSGHWIYWV 735
            R  + HW+YWV
Sbjct:  196 RNFTNHWVYWV 206

HSP 3 Score: 23.483 bits (49), Expect = 5.769e-18
Identity = 9/16 (56.25%), Postives = 11/16 (68.75%), Query Frame = 2 
Query:  665 MNPXPSFGPAVASGNF 712
            MNP  SF PA+ + NF
Sbjct:  183 MNPARSFAPAILTRNF 198
[Close]
BLAST of EY756300 vs. ExPASy Swiss-Prot
Match: MIP_RANPI (Lens fiber major intrinsic protein OS=Rana pipiens PE=1 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 5.769e-18
Identity = 54/171 (31.58%), Postives = 87/171 (50.88%), Query Frame = 3 
Query:  120 SLGSFKAYLAEFISTLLFVFAGVGSAIAFNKMTADAALDPSGLVAIAICHGFALFVAVAIGANISGGHVNPAVTFGLALGGQITILTGIFYWIAQLLGSIVASFLLKVVT-----GGLAVPTHNXXXXXXXXXXXXXXXXXTFGLVYTVYATAADPKKGSLGTIAPIAIGF 617
            S   ++A  AEF  T+ +VF G+G+++ +       A  P+ ++ IA+  G  L   V    ++SG H+NPAVTF   +G Q+++   IFY  AQLLG++  + +L  VT     G LA+ T                   T   V  ++AT  + + G LG+++ +AIGF
Sbjct:    6 SFSFWRAVFAEFFGTMFYVFFGLGASLKW-------AAGPANVLVIALAFGLVLATMVQSIGHVSGAHINPAVTFAFLIGSQMSLFRAIFYIAAQLLGAVAGAAVLYGVTPAAIRGNLALNT--LHPGVSLGQATTVEIFLTLQFVLCIFATYDERRNGRLGSVS-LAIGF 166

HSP 2 Score: 23.8682 bits (50), Expect = 5.769e-18
Identity = 10/16 (62.50%), Postives = 11/16 (68.75%), Query Frame = 2 
Query:  665 MNPXPSFGPAVASGNF 712
            MNP  SF PAV + NF
Sbjct:  183 MNPARSFAPAVLTRNF 198

HSP 3 Score: 23.8682 bits (50), Expect = 5.769e-18
Identity = 6/11 (54.55%), Postives = 8/11 (72.73%), Query Frame = 1 
Query:  703 RQLSGHWIYWV 735
            R  + HW+YWV
Sbjct:  196 RNFTNHWVYWV 206
[Close]
BLAST of EY756300 vs. ExPASy Swiss-Prot
Match: MIP_RAT (Lens fiber major intrinsic protein (Fragment) OS=Rattus norvegicus GN=Mip PE=1 SV=2)

HSP 1 Score: 82.4185 bits (202), Expect = 5.773e-18
Identity = 58/186 (31.18%), Postives = 90/186 (48.39%), Query Frame = 3 
Query:  120 SLGSFKAYLAEFISTLLFVFAGVGSAIAFNKMTADAALDPSGLVAIAICHGFALFVAVAIGANISGGHVNPAVTFGLALGGQITILTGIFYWIAQLLGSIVASFLLKVVT-----GGLAVPTHNXXXXXXXXXXXXXXXXXTFGLVYTVYATAADPKKGSLGTIA-----PIAIGFIVGANIPGAG 647
            S   ++A  AEF +TL +VF G+GS++ +       A  P  ++ +A+  G AL   V    +ISG HVNPAVTF   +G Q+++L    Y  AQLLG++  + +L  VT     G LA+ T                   T   V  ++AT  + + G +G++A      + +G + G    GAG
Sbjct:    4 SASFWRAIFAEFFATLFYVFFGLGSSLRW-------APGPLHVLQVALAFGLALATLVQTVGHISGAHVNPAVTFAFLVGSQMSLLRAFCYIAAQLLGAVAGAAVLYSVTPPAVRGNLALNT--LHAGVSVGQATTVEIFLTLQFVLCIFATYDERRNGRMGSVALAVGFSLTLGHLFGMYYTGAG 180

HSP 2 Score: 25.0238 bits (53), Expect = 5.773e-18
Identity = 7/11 (63.64%), Postives = 8/11 (72.73%), Query Frame = 1 
Query:  703 RQLSGHWIYWV 735
            R  S HW+YWV
Sbjct:  194 RNFSNHWVYWV 204

HSP 3 Score: 23.8682 bits (50), Expect = 5.773e-18
Identity = 9/18 (50.00%), Postives = 12/18 (66.67%), Query Frame = 2 
Query:  665 MNPXPSFGPAVASGNFQD 718
            MNP  SF PA+ + NF +
Sbjct:  181 MNPARSFAPAILTRNFSN 198
[Close]
BLAST of EY756300 vs. ExPASy Swiss-Prot
Match: AQP4_DIPME (Aquaporin-4 OS=Dipodomys merriami GN=AQP4 PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 6.298e-18
Identity = 68/212 (32.08%), Postives = 104/212 (49.06%), Query Frame = 3 
Query:  132 FKAYLAEFISTLLFVFAGVGSAIAFNKMTADAALDPSGLVAIAICHGFALFVAVAIGANISGGHVNPAVTFGLALGGQITILTGIFYWIAQLLGSIVASFLLKVVT-----GGLAVPTHNXXXXXXXXXXXXXXXXXTFGLVYTVYATAADPKKGSLGTIAPIAIGFIVGANIPGAGPFSGGS*TXXRPSGLPLPAATFRTLDLLG-WPSHW 749
            +KA  AEF++ L+FV   +GS I +        +D   +V I++C G ++   V    +ISGGH+NPAVT  +    +I+I   +FY  AQ LG+I+ + +L +VT     GGL V T                   TF LV+T++A+    +    G+IA +AIGF V      A  ++G S           PA +F    ++G W +HW
Sbjct:   35 WKAVTAEFLAMLIFVLLSLGSTINWGGKENPLPVD---MVLISLCFGLSIATMVQCFGHISGGHINPAVTVAMVCTRKISIAKSVFYIAAQCLGAIIGAGILYLVTPPSVVGGLGVTT--VHGNLTAGHGLLVELIITFQLVFTIFASCDSKRTDVTGSIA-LAIGFSVAIGHLFAINYTGASMN---------PARSFGPAVIMGNWENHW 231
[Close]
BLAST of EY756300 vs. ExPASy Swiss-Prot
Match: AQP1_MOUSE (Aquaporin-1 OS=Mus musculus GN=Aqp1 PE=1 SV=3)

HSP 1 Score: 85.1149 bits (209), Expect = 7.464e-18
Identity = 57/167 (34.13%), Postives = 85/167 (50.90%), Query Frame = 3 
Query:  132 FKAYLAEFISTLLFVFAGVGSAIAFNKMTADAALDPSGLVAIAICHGFALFVAVAIGANISGGHVNPAVTFGLALGGQITILTGIFYWIAQLLGSIVASFLLKVVTGGL---AVPTHNXXXXXXXXXXXXXXXXXTFGLVYTVYATAADPKKGSLGTIAPIAIGFIV 623
            ++A +AEF++  LFVF  +GSA+ FN             V +++  G ++        +ISG H+NPAVT GL L  QI+IL  + Y IAQ +G+IVA+ +L  +T  L   ++  ++                 T  LV  V AT  D ++  LG  AP+AIG  V
Sbjct:   11 WRAVVAEFLAMTLFVFISIGSALGFNYPLERNQTLVQDNVKVSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISILRAVMYIIAQCVGAIVATAILSGITSSLVDNSLGRNDLAHGVNSGQGLGIEIIGTLQLVLCVLAT-TDRRRRDLGGSAPLAIGLSV 176

HSP 2 Score: 23.8682 bits (50), Expect = 7.464e-18
Identity = 7/11 (63.64%), Postives = 8/11 (72.73%), Query Frame = 1 
Query:  703 RQLSGHWIYWV 735
            R  S HWI+WV
Sbjct:  204 RNFSNHWIFWV 214

HSP 3 Score: 21.9422 bits (45), Expect = 7.464e-18
Identity = 9/18 (50.00%), Postives = 12/18 (66.67%), Query Frame = 2 
Query:  665 MNPXPSFGPAVASGNFQD 718
            +NP  SFG AV + NF +
Sbjct:  191 INPARSFGSAVLTRNFSN 208
[Close]
BLAST of EY756300 vs. ExPASy Swiss-Prot
Match: PIP25_ORYSJ (Aquaporin PIP2-5 OS=Oryza sativa subsp. japonica GN=PIP2-5 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 8.225e-18
Identity = 60/178 (33.71%), Postives = 91/178 (51.12%), Query Frame = 3 
Query:  132 FKAYLAEFISTLLFVFAGVGSAIAFNKMTA------DAALDPSGLVAIAICHGFALFVAVAIGANISGGHVNPAVTFGLALGGQITILTGIFYWIAQLLGSIVASFLLKVVTGGL----AVPTHNXXXXXXXXXXXXXXXXXTFGLVYTVYATAADPKKGS----LGTIAPIAIGFIV 623
            ++A +AEF++TLLF++  V + I +   T       DAA    G++ IA   G  +F+ V   A ISGGH+NPAVTFGL L  +++++  I Y +AQ LG++    L+K              +                  TF LVYTV+ +A DPK+ +    +  +AP+ IGF V
Sbjct:   35 YRAVIAEFVATLLFLYVTVATVIGYKHQTDASASGDDAACGGVGVLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRAILYIVAQCLGAVCGVALVKGFQSSFYDRYGGGANELAAGYSKGTGLAAEIIGTFVLVYTVF-SATDPKRNARDSHVPVLAPLPIGFAV 211
[Close]
BLAST of EY756300 vs. ExPASy Swiss-Prot
Match: PIP24_ORYSJ (Aquaporin PIP2-4 OS=Oryza sativa subsp. japonica GN=PIP2-4 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 8.225e-18
Identity = 60/178 (33.71%), Postives = 91/178 (51.12%), Query Frame = 3 
Query:  132 FKAYLAEFISTLLFVFAGVGSAIAFNKMT------ADAALDPSGLVAIAICHGFALFVAVAIGANISGGHVNPAVTFGLALGGQITILTGIFYWIAQLLGSIVASFLLKVVTGGL----AVPTHNXXXXXXXXXXXXXXXXXTFGLVYTVYATAADPKKGS----LGTIAPIAIGFIV 623
            ++A +AEF++TLLF++  V + I +   T      ADAA    G++ IA   G  +F+ V   A +SGGH+NPAVT GL L  +++++  + Y  AQ LG+I    L+K     L        +                  TF LVYTV+ +A DPK+ +    +  +AP+ IGF V
Sbjct:   38 YRALIAEFVATLLFLYVTVATVIGYKHQTDAAVNGADAACGGVGVLGIAWAFGGMIFILVYCTAGVSGGHINPAVTLGLFLARKVSLVRALLYMAAQCLGAICGVALVKGFQSSLYDRYGGGANELAAGYSTGTGLAAEIIGTFVLVYTVF-SATDPKRNARDSHVPVLAPLPIGFAV 214
[Close]
BLAST of EY756300 vs. ExPASy Swiss-Prot
Match: MIP_MOUSE (Lens fiber major intrinsic protein OS=Mus musculus GN=Mip PE=1 SV=2)

HSP 1 Score: 81.2629 bits (199), Expect = 1.254e-17
Identity = 57/186 (30.65%), Postives = 90/186 (48.39%), Query Frame = 3 
Query:  120 SLGSFKAYLAEFISTLLFVFAGVGSAIAFNKMTADAALDPSGLVAIAICHGFALFVAVAIGANISGGHVNPAVTFGLALGGQITILTGIFYWIAQLLGSIVASFLLKVVT-----GGLAVPTHNXXXXXXXXXXXXXXXXXTFGLVYTVYATAADPKKGSLGTIA-----PIAIGFIVGANIPGAG 647
            S   ++A  AEF +TL +VF G+G+++ +       A  P  ++ +A+  G AL   V    +ISG HVNPAVTF   +G Q+++L    Y  AQLLG++  + +L  VT     G LA+ T                   T   V  ++AT  + + G +G++A      + +G + G    GAG
Sbjct:    6 SASFWRAIFAEFFATLFYVFFGLGASLRW-------APGPLHVLQVALAFGLALATLVQTVGHISGAHVNPAVTFAFLVGSQMSLLRAFCYIAAQLLGAVAGAAVLYSVTPPAVRGNLALNT--LHAGVSVGQATTVEIFLTLQFVLCIFATYDERRNGRMGSVALAVGFSLTLGHLFGMYYTGAG 182

HSP 2 Score: 25.0238 bits (53), Expect = 1.254e-17
Identity = 7/11 (63.64%), Postives = 8/11 (72.73%), Query Frame = 1 
Query:  703 RQLSGHWIYWV 735
            R  S HW+YWV
Sbjct:  196 RNFSNHWVYWV 206

HSP 3 Score: 23.8682 bits (50), Expect = 1.254e-17
Identity = 9/18 (50.00%), Postives = 12/18 (66.67%), Query Frame = 2 
Query:  665 MNPXPSFGPAVASGNFQD 718
            MNP  SF PA+ + NF +
Sbjct:  183 MNPARSFAPAILTRNFSN 200
[Close]
BLAST of EY756300 vs. ExPASy Swiss-Prot
Match: AQP1_HUMAN (Aquaporin-1 OS=Homo sapiens GN=AQP1 PE=1 SV=3)

HSP 1 Score: 85.8853 bits (211), Expect = 1.622e-17
Identity = 56/167 (33.53%), Postives = 85/167 (50.90%), Query Frame = 3 
Query:  132 FKAYLAEFISTLLFVFAGVGSAIAFNKMTADAALDPSGLVAIAICHGFALFVAVAIGANISGGHVNPAVTFGLALGGQITILTGIFYWIAQLLGSIVASFLLKVVTGGL---AVPTHNXXXXXXXXXXXXXXXXXTFGLVYTVYATAADPKKGSLGTIAPIAIGFIV 623
            ++A +AEF++T LFVF  +GSA+ F     +        V +++  G ++        +ISG H+NPAVT GL L  QI+I   + Y IAQ +G+IVA+ +L  +T  L   ++  ++                 T  LV  V AT  D ++  LG  AP+AIG  V
Sbjct:   11 WRAVVAEFLATTLFVFISIGSALGFKYPVGNNQTAVQDNVKVSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISIFRALMYIIAQCVGAIVATAILSGITSSLTGNSLGRNDLADGVNSGQGLGIEIIGTLQLVLCVLAT-TDRRRRDLGGSAPLAIGLSV 176

HSP 2 Score: 21.9422 bits (45), Expect = 1.622e-17
Identity = 9/18 (50.00%), Postives = 12/18 (66.67%), Query Frame = 2 
Query:  665 MNPXPSFGPAVASGNFQD 718
            +NP  SFG AV + NF +
Sbjct:  191 INPARSFGSAVITHNFSN 208

HSP 3 Score: 21.9422 bits (45), Expect = 1.622e-17
Identity = 6/8 (75.00%), Postives = 7/8 (87.50%), Query Frame = 1 
Query:  712 SGHWIYWV 735
            S HWI+WV
Sbjct:  207 SNHWIFWV 214
The following BLAST results are available for this feature:
BLAST of EY756300 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 154
Match NameE-valueIdentityDescription
AQP1_SHEEP5.759e-1833.72Aquaporin-1 OS=Ovis aries GN=AQP1 PE=2 SV=3[more]
MIP_BOVIN5.769e-1831.18Lens fiber major intrinsic protein OS=Bos taurus G... [more]
MIP_RANPI5.769e-1831.58Lens fiber major intrinsic protein OS=Rana pipiens... [more]
MIP_RAT5.773e-1831.18Lens fiber major intrinsic protein (Fragment) OS=R... [more]
AQP4_DIPME6.298e-1832.08Aquaporin-4 OS=Dipodomys merriami GN=AQP4 PE=2 SV=... [more]
AQP1_MOUSE7.464e-1834.13Aquaporin-1 OS=Mus musculus GN=Aqp1 PE=1 SV=3[more]
PIP25_ORYSJ8.225e-1833.71Aquaporin PIP2-5 OS=Oryza sativa subsp. japonica G... [more]
PIP24_ORYSJ8.225e-1833.71Aquaporin PIP2-4 OS=Oryza sativa subsp. japonica G... [more]
MIP_MOUSE1.254e-1730.65Lens fiber major intrinsic protein OS=Mus musculus... [more]
AQP1_HUMAN1.622e-1733.53Aquaporin-1 OS=Homo sapiens GN=AQP1 PE=1 SV=3[more]

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Properties
Property NameValue
Genbank descriptionCS13-C1-001-007-A07-CT.F Sweet orange leaf, field plant B Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY756300 ID=EY756300; Name=EY756300; organism=Citrus sinensis; type=EST; length=899bp
AGATAAAACCTTATCTTCTTCTTCTTAAGAAAGACTCGAGAGAGTTTTTT
TTTCCCTATTCTCTAGCTCTTTCTTGATCAACAATGGCTATTGCCTTCGG
CCGCTTCGATGATTCTTTCAGCTTAGGCTCCTTCAAGGCCTATCTTGCTG
AGTTTATCTCAACTTTGCTCTTTGTTTTCGCCGGCGTTGGCTCGGCCATT
GCTTTCAACAAGATGACAGCAGATGCAGCACTTGATCCATCAGGGCTAGT
AGCCATCGCTATTTGCCATGGATTTGCTCTATTTGTCGCAGTTGCAATTG
GTGCTAACATCTCAGGTGGCCACGTCAACCCAGCCGTCACCTTTGGATTG
GCTCTTGGTGGCCAAATCACCATCCTCACTGGCATCTTCTACTGGATTGC
CCAACTTCTTGGCTCTATTGTTGCCAGCTTCCTCCTCAAAGTTGTCACTG
GAGGCTTGGCAGTTCCTACCCACAATGTTGCTGCTGGAGTTGGAGCCATT
GAAGGAGTTGTGATGGAGATCATCATCACATTTGGTTTGGTCTACACGGT
CTATGCCACGGCAGCTGACCCCAAGAAGGGATCATTGGGCACCATTGCAC
CCATTGCCATCGGTTTCATTGTCGGTGCCAACATCCCTGGCGCCGGCCCA
TTCTCCGGTGGTTCATGAACCCNGNCCCGTCCTTCGGGCCTGCCGTTGCC
AGCGGCAACTTTCAGGACACTGGATCTACTGGGTTGGCCCTCTCATTGGC
GNTGGCCTTGCCGGGCTCATCTATGGCAATGTGNTCATGCACTCTGAACA
TGCACCCCTATCNTATGACTATTAATTANTAAGTAAACTTTGNTTAAAAG
TTAGTAAATCATTTGATCACTGCTTTTGGCTCTTTCTTTTTGAATAAAA
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