EY756032

Overview
NameEY756032
Unique NameEY756032
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length852
Libraries
Library NameType
Sweet orange leaf, field plant Bcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1827 contig Csv1_Contig1827:34..885. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of EY756032 vs. ExPASy Swiss-Prot
Match: IF1A_SULIA (Translation initiation factor 1A OS=Sulfolobus islandicus (strain M.16.27) GN=eIF1A PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.763e-12
Identity = 32/80 (40.00%), Postives = 51/80 (63.75%), Query Frame = 3
Query:  216 RELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLL 455
            R++   E+GQ    V +MLG      +C+DG +RL  I GK+ KK+W+  GD++LVG+ D+Q ++ D++ KY  DE + L
Sbjct:   13 RDVPKPEEGQTICVVKKMLGGDHLVVLCMDGKERLARIPGKIRKKMWMREGDVVLVGIWDFQPNRCDILYKYGNDEIKRL 92          
BLAST of EY756032 vs. ExPASy Swiss-Prot
Match: IF1A_METKA (Translation initiation factor 1A OS=Methanopyrus kandleri GN=eIF1A PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.763e-12
Identity = 34/92 (36.96%), Postives = 56/92 (60.87%), Query Frame = 3
Query:  204 DDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPE 479
            D+  +++   ++G+ +  V +MLGN R +  C+DG  R+  I GKM K+VWI  GD++LV   ++Q ++ADV  +Y   +   LK  G+L E
Sbjct:    7 DETLKKIRLPKEGEIFGVVEKMLGNDRVQVRCVDGKTRVARIPGKMRKRVWIREGDVVLVKPWEFQPERADVTWRYTRVQVDWLKRKGKLDE 98          
BLAST of EY756032 vs. ExPASy Swiss-Prot
Match: IF1A_SULSO (Translation initiation factor 1A OS=Sulfolobus solfataricus GN=eIF1A PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.608e-12
Identity = 32/80 (40.00%), Postives = 51/80 (63.75%), Query Frame = 3
Query:  216 RELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLL 455
            R++   E+GQ    V +MLG      +C+DG +RL  I GK+ KK+W+  GD++LVG+ D+Q ++ D++ KY  DE + L
Sbjct:   13 RDVPRPEEGQTICVVKKMLGGDHLIVLCMDGKERLARIPGKIRKKMWMREGDVVLVGIWDFQPNRCDILYKYGNDEIKRL 92          
BLAST of EY756032 vs. ExPASy Swiss-Prot
Match: IF1A_HALS3 (Translation initiation factor 1A OS=Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) GN=eIF1A PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.608e-12
Identity = 35/87 (40.23%), Postives = 51/87 (58.62%), Query Frame = 3
Query:  213 KRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGEL 473
            ++ L   ED + +A+V  MLG  R +  C DG +R   I G+M K++WI   DI+LV   D+QDDKADV  +Y   +A  L+  G +
Sbjct:    8 RKNLRMPEDDEVFAEVTDMLGANRVQVRCADGEERTARIPGRMQKRIWIREDDIVLVEPWDWQDDKADVTWRYEKSDADQLREEGHI 94          
BLAST of EY756032 vs. ExPASy Swiss-Prot
Match: IF1A1_HALSA (Translation initiation factor 1A 1 OS=Halobacterium salinarium GN=eIF1A1 PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.608e-12
Identity = 35/87 (40.23%), Postives = 51/87 (58.62%), Query Frame = 3
Query:  213 KRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGEL 473
            ++ L   ED + +A+V  MLG  R +  C DG +R   I G+M K++WI   DI+LV   D+QDDKADV  +Y   +A  L+  G +
Sbjct:    8 RKNLRMPEDDEVFAEVTDMLGANRVQVRCADGEERTARIPGRMQKRIWIREDDIVLVEPWDWQDDKADVTWRYEKSDADQLREEGHI 94          
BLAST of EY756032 vs. ExPASy Swiss-Prot
Match: IF1A_PICTO (Translation initiation factor 1A OS=Picrophilus torridus GN=eif1a PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.791e-11
Identity = 34/83 (40.96%), Postives = 48/83 (57.83%), Query Frame = 3
Query:  231 KEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPE 479
            K+ G+ Y  V +M G  R   MC DG  R C I GKM K++WI  GD+++V   ++QD+K D+I +Y   +A  L     LPE
Sbjct:   17 KKSGEIYGIVEKMSGASRLIVMCEDGVTRNCRIPGKMKKRMWIREGDLVIVKPWEFQDEKGDIIYRYTKTQAAYLSRNHMLPE 99          
BLAST of EY756032 vs. ExPASy Swiss-Prot
Match: IF1A1_HALWD (Translation initiation factor 1A 1 OS=Haloquadratum walsbyi (strain DSM 16790) GN=eif1a1 PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 2.339e-11
Identity = 35/94 (37.23%), Postives = 52/94 (55.32%), Query Frame = 3
Query:  201 ADDEKR---ELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGEL 473
            +DDE R   +L   +D + +A V  MLG  R    C DG +R   I G+M K++WI   D++LV   D+QD+K D+  +Y   EA  L+  G +
Sbjct:    2 SDDENRGSHDLRMPDDDEVFAVVTNMLGANRVTVRCADGNERTARIPGRMQKRIWIREDDVVLVEPWDWQDEKGDITWRYEKSEADQLREEGRI 95          
BLAST of EY756032 vs. ExPASy Swiss-Prot
Match: IF1A_METLZ (Translation initiation factor 1A OS=Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) GN=eIF1A PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 8.887e-11
Identity = 31/74 (41.89%), Postives = 46/74 (62.16%), Query Frame = 3
Query:  243 QEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAY 464
            +++AQ   MLG+      C DG  RL  I+GK+ K+VWI  GDI++V   D+QDDK D+I +Y   +   L+A+
Sbjct:   29 EQFAQAELMLGSNHIRVRCSDGVTRLGRIKGKIKKRVWIREGDILIVVPWDFQDDKCDIIYRYTTPQVDWLRAH 102          
BLAST of EY756032 vs. ExPASy Swiss-Prot
Match: IF1A_DESK1 (Translation initiation factor 1A OS=Desulfurococcus kamchatkensis (strain 1221n / DSM 18924) GN=eIF1A PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 8.887e-11
Identity = 34/82 (41.46%), Postives = 47/82 (57.32%), Query Frame = 3
Query:  234 EDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPE 479
            ++G     V+R LG     A C+DG  R   I GK+ +KVWI  GDIILVGL D+  +K +V+ KY  +E   L   G +P+
Sbjct:   25 DEGTIICGVVRHLGGDYLIAKCLDGVDRKIRIPGKLRRKVWITEGDIILVGLWDFSSEKGEVVYKYGKNEVNKLVEKGVVPK 106          
The following BLAST results are available for this feature:
BLAST of EY756032 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 29
Match NameE-valueIdentityDescription
IF1A_SULIA2.763e-1240.00Translation initiation factor 1A OS=Sulfolobus isl... [more]
IF1A_METKA2.763e-1236.96Translation initiation factor 1A OS=Methanopyrus k... [more]
IF1A_SULSO3.608e-1240.00Translation initiation factor 1A OS=Sulfolobus sol... [more]
IF1A_HALS33.608e-1240.23Translation initiation factor 1A OS=Halobacterium ... [more]
IF1A1_HALSA3.608e-1240.23Translation initiation factor 1A 1 OS=Halobacteriu... [more]
IF1A_PICTO1.791e-1140.96Translation initiation factor 1A OS=Picrophilus to... [more]
IF1A1_HALWD2.339e-1137.23Translation initiation factor 1A 1 OS=Haloquadratu... [more]
IF1A_METLZ8.887e-1141.89Translation initiation factor 1A OS=Methanocorpusc... [more]
IF1A_DESK18.887e-1141.46Translation initiation factor 1A OS=Desulfurococcu... [more]

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Properties
Property NameValue
Genbank descriptionCS13-C1-001-002-G12-CT.F Sweet orange leaf, field plant B Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY756032 ID=EY756032; Name=EY756032; organism=Citrus sinensis; type=EST; length=852bp
CCCGCGTCCGGCCGACAGTTTGCACCTGATCTTTTCGGTACAAAAACGCT
AGGGTTCGTAGTTATAACCTCTCAGAAACAGATTGATTTCAATCTTGCTG
CTTTTTAACTCTCCGACTCCACCTCTCCCCCAAAGAATCAGAAACCATGC
CGAAGAATAAGGGAAAGGGAGGAAAGAACAGGAAGAGGGGAAAGAATGAA
GCTGACGACGAGAAGCGTGAGCTTGTCTTCAAGGAAGACGGACAGGAATA
TGCCCAAGTCCTTCGTATGCTCGGCAATGGTCGGTGTGAAGCTATGTGCA
TCGATGGAACCAAGCGTCTTTGCCACATCCGTGGGAAGATGCACAAAAAG
GTTTGGATTGCAGCTGGTGATATAATACTTGTTGGCCTACGTGATTATCA
GGATGACAAGGCTGATGTCATCCTCAAGTATATGCCCGACGAGGCAAGGC
TTCTGAAGGCTTATGGTGAACTTCCAGAGAGTACCCGTCTCAATGAGGGC
ATTGCTGGTGGTCTTGATGAAGAGGATGATGGCGCTGATAATGATTACAT
CGAGTTTGAAGACGAAGATATTGATAAGATCTAGGCTCCGAGAATCGACT
CTGTCTGGGGTTTATGTTCAATATTTAGCAAAAATGGAGTATGCCAATGT
ATTGGTTACTTAAAAGCTGAAAATATGTGTTTGCAGCTTATATATTTGGA
ATTTTTAATGGTGAGCCTGTCATATTTCGTGTAACGCCCTATACTTGCTT
ATTAATTCTTGTTAGTTCTGCACTTTTTTTGAAAGTGGATTTCTAGAATG
GAATCTTATACTGGTAAAAANNNCCCCCCACCCCCCCCCCCCCCCCCCNC
NC
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