EY728837

Overview
NameEY728837
Unique NameEY728837
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length901
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_THERP (Glucose-1-phosphate adenylyltransferase OS=Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) GN=glgC PE=3 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 3.426e-23
Identity = 56/174 (32.18%), Postives = 97/174 (55.75%), Query Frame = 2 
Query:   41 IIQAHRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLNLLRDKFPGAN---DFGSEVIPGATSIGMRVQAYLYDGYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKMLD 553
            +I  HRE  AD+T+A  P+D + A+ FG++ + E+G +++F EKP+                     +    +ASMGIY+  ++++L+L     P A    DFG +VIP       RV  Y +DGYW+D+GT+++++ AN+ + +   P  + YD +  I+T+    PP+K+L+
Sbjct:  137 MIAQHRERCADVTIAVQPVDWREASRFGVVIVAEDGWVVDFEEKPE---------------------RPRSNLASMGIYLFRRNLLLDLFTRDHPDAPEFIDFGRDVIPYLIRTA-RVATYRFDGYWQDVGTVQSYWEANMALLEDE-PKLNLYDPNWRIHTRSEERPPAKILE 287

HSP 2 Score: 27.335 bits (59), Expect = 3.426e-23
Identity = 9/43 (20.93%), Postives = 23/43 (53.49%), Query Frame = 3 
Query:  576 LGEGCVIKNCKIHHSVVGLRSCISEGAIIEDTLLMGSRLL*DG 704
            L  GC+++   +  S++     +  GA++ D+++M   ++  G
Sbjct:  296 LSNGCIVEGATVIRSILSPGVIVKAGAVVRDSIVMTDSVIGPG 338
[Close]
BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_NITOC (Glucose-1-phosphate adenylyltransferase OS=Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) GN=glgC PE=3 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 7.549e-23
Identity = 63/181 (34.81%), Postives = 95/181 (52.49%), Query Frame = 2 
Query:   38 EIIQAHRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLNLL---RDKFPGANDFGSEVIPGATSIGMRVQAYLYD-------GYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKML 550
            +++  H E +AD+TV  + +  K A AFG+M +DE  R+ EF+EKP+  Q    + D T              +ASMGIYV +   +   L    D F  ++DFG ++IP       RV A+ +        GYW D+GT++AF+NANL +     P+ + YD   PI+T    LPP+K +
Sbjct:  147 DMLAYHVEREADMTVGCIHVPLKEAKAFGVMSVDENFRVTEFTEKPEHPQPSPGRSDET--------------LASMGIYVFNAAFLYEQLIKNADAFNSSHDFGKDIIPSILRSHYRVIAFPFSDVQGGDPGYWRDVGTVDAFWNANLELIGVS-PELNLYDEDWPIWTYQAQLPPAKFI 312

HSP 2 Score: 22.3274 bits (46), Expect = 7.549e-23
Identity = 9/18 (50.00%), Postives = 13/18 (72.22%), Query Frame = 3 
Query:   12 DHLYRMDY-ERLFRHTEK 62
            DH+Y+MDY + L  H E+
Sbjct:  138 DHVYKMDYGDMLAYHVER 155
[Close]
BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_ANADF (Glucose-1-phosphate adenylyltransferase OS=Anaeromyxobacter sp. (strain Fw109-5) GN=glgC PE=3 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.216e-22
Identity = 63/198 (31.82%), Postives = 108/198 (54.55%), Query Frame = 2 
Query:   38 EIIQAHRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVML-NLLRD--KFPGANDFGSEVIPGATSIGMRVQAYLYD--------------GYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKMLDADVTDSVIG 580
            +++Q H++  AD+TVAA+P+  + A+ FG++++D +GR+I F EKPK   +K M  D T       RA     +ASMG Y+ + D ++  ++RD      A+DFG  ++    ++  R + ++YD              GYW D+G+++A+Y AN+ +     P FS Y+   PI+T     PP K +  + T+  +G
Sbjct:  133 QMLQFHQDKGADLTVAAIPVPVEEASEFGIIEVDGDGRMIGFVEKPKA-GVKTMPGDPT-------RA-----LASMGNYLFTTDALVQEIVRDAGDTKSAHDFGKSIV---AAMYERKRVFVYDFAKNVVPGQGDKERGYWRDVGSLDAYYQANMDLVDVD-PSFSLYNDRWPIFTAQHNFPPVKFVFNNQTEGRVG 313

HSP 2 Score: 28.4906 bits (62), Expect = 1.216e-22
Identity = 10/34 (29.41%), Postives = 21/34 (61.76%), Query Frame = 3 
Query:  576 LGEGCVIKNCKIHHSVVGLRSCISEGAIIEDTLL 677
            + EGC+I     HH ++  +  I+  +++ED++L
Sbjct:  320 VSEGCIISGGHAHHCILSPKVRINSYSLVEDSIL 353

HSP 3 Score: 21.1718 bits (43), Expect = 1.216e-22
Identity = 8/18 (44.44%), Postives = 13/18 (72.22%), Query Frame = 3 
Query:   12 DHLYRMDYERLFR-HTEK 62
            DH+YRMD  ++ + H +K
Sbjct:  124 DHVYRMDVRQMLQFHQDK 141
[Close]
BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_THET2 (Glucose-1-phosphate adenylyltransferase OS=Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) GN=glgC PE=3 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 1.233e-21
Identity = 63/185 (34.05%), Postives = 100/185 (54.05%), Query Frame = 2 
Query:   41 IIQAHRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLNLL----RDKFPGANDFGSEVIPGATSIGMRVQAYLY-----------DGYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKML 550
            +++ H +T ADIT+AA P+    AT FG++++DEE RI EF EKP+  +    + DT               +ASMG Y+   + +  LL    RD+   A+DFG +VIP A   G RV AY +           + YW D+GT++A+Y A++ +  K +P+F  ++   P+ T   + PP+K +
Sbjct:  141 MVEYHYDTRADITIAAYPVPVAEATRFGVLQVDEEWRITEFQEKPEEPKPIPGRPDTA--------------LASMGNYIFRTEALFELLEADARDE-TSAHDFGKDVIPRALREGYRVYAYDFHRNPIPGQEGPNLYWRDVGTLDAYYEASMDLV-KVVPEFDLFNPEWPLRTANLFSPPAKFV 309
[Close]
BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_THEAB (Glucose-1-phosphate adenylyltransferase OS=Thermosipho africanus (strain TCF52B) GN=glgC PE=3 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 1.633e-21
Identity = 58/176 (32.95%), Postives = 93/176 (52.84%), Query Frame = 2 
Query:   38 EIIQAHRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLNLLRD---KFPGANDFGSEVIPGATSIGMRVQAYLYDGYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKMLDA 556
            E++  H+   A  TVA + +    A+ FG+M  D E RIIEF EKPK                     +    +AS+GIYV     +  +L +       ++DFG ++IP       RV A+ +DGYW+D+GTI +++ +NL +T +PIP F+ +D +  IYT  + +PP+ + D+
Sbjct:  135 ELLDYHKAKSALATVACMEVPLSEASRFGIMVTDLENRIIEFQEKPK---------------------QPKSTLASLGIYVFQWSFIREVLIEDAKNDQSSHDFGKDIIPKIIQT-ERVFAFPFDGYWKDVGTIYSYWESNLELT-RPIPPFNIHDENWRIYTHSKEMPPAYIADS 287

HSP 2 Score: 26.5646 bits (57), Expect = 1.633e-21
Identity = 12/36 (33.33%), Postives = 22/36 (61.11%), Query Frame = 3 
Query:  576 LGEGCVIKNCKIHHSVVGLRSCISEGAIIEDTLLMG 683
            +G+G ++KN  I  +V    +C  E AII + +++G
Sbjct:  316 IGKGSIVKNSVIMSNVRIGENCYIENAIIAENVVIG 351

HSP 3 Score: 21.557 bits (44), Expect = 1.633e-21
Identity = 7/11 (63.64%), Postives = 8/11 (72.73%), Query Frame = 3 
Query:   12 DHLYRMDYERL 44
            DH+Y MDY  L
Sbjct:  126 DHIYSMDYNEL 136
[Close]
BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_THEM4 (Glucose-1-phosphate adenylyltransferase OS=Thermosipho melanesiensis (strain BI429 / DSM 12029) GN=glgC PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 2.118e-21
Identity = 62/185 (33.51%), Postives = 99/185 (53.51%), Query Frame = 2 
Query:   38 EIIQAHRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVIS----KDVMLNLLRDKFPGANDFGSEVIPGATSIGMRVQAYLYDGYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKML-DADVTDSVI 577
            E++  H    A  TVA + +    A  FG+M  D E RIIEF EKPK  +                       +AS+GIYV      ++V++   +D+    +DFG ++IP   +   RV A+ ++GYW+D+GTI +++ +NL +T +PIP F+ +D +  IYT    +PP+ +  DA V +S+I
Sbjct:  135 ELVDYHVAKSALGTVACMEVPLSEANRFGIMVTDLENRIIEFQEKPKFPK---------------------STLASLGIYVFQWNFIREVLMEDAKDE-NSTHDFGKDIIPKIINT-KRVYAFPFEGYWKDVGTIYSYWESNLELT-RPIPPFNIHDENWKIYTHSEEMPPAYISDDARVKNSLI 295

HSP 2 Score: 28.1054 bits (61), Expect = 2.118e-21
Identity = 13/39 (33.33%), Postives = 23/39 (58.97%), Query Frame = 3 
Query:  576 LGEGCVIKNCKIHHSVVGLRSCISEGAIIEDTLLMGSRL 692
            +GEG +IKN  +   V    +C  E AII + +++G+ +
Sbjct:  316 VGEGVIIKNSVVMSRVRIGNNCFIENAIIAENVVIGNEV 354

HSP 3 Score: 21.557 bits (44), Expect = 2.118e-21
Identity = 7/11 (63.64%), Postives = 8/11 (72.73%), Query Frame = 3 
Query:   12 DHLYRMDYERL 44
            DH+Y MDY  L
Sbjct:  126 DHIYSMDYNEL 136
[Close]
BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_CLOCE (Glucose-1-phosphate adenylyltransferase OS=Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) GN=glgC PE=3 SV=2)

HSP 1 Score: 94.3597 bits (233), Expect = 2.742e-21
Identity = 56/185 (30.27%), Postives = 93/185 (50.27%), Query Frame = 2 
Query:   38 EIIQAHRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLN-LLRDK--FPGANDFGSEVIPGATSIGMRVQAYLYDGYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKML--DADVTDSVI 577
            +++  H+E  AD T++ + +  + A+ +G+M   E G+I EF EKPK  +                       +ASMG+Y+ +   +   L++D       NDFG  +IP     G  + AY Y GYW D+GTI+AF+ +N+ +  + +P F+ +D    IYT P  + P+  +   A V  S+I
Sbjct:  137 KMLDFHKENHADATISVINVPYEEASRYGIMNCHENGKIYEFEEKPKNPKST---------------------LASMGVYIFTWSTLREYLIKDNECSDSVNDFGKNIIPAMLGDGKSMWAYQYSGYWRDVGTIQAFWESNMDLVSR-VPQFNLFDPEWRIYT-PNPVKPAHYIASSACVKKSII 298

HSP 2 Score: 26.1794 bits (56), Expect = 2.742e-21
Identity = 12/37 (32.43%), Postives = 24/37 (64.86%), Query Frame = 3 
Query:  576 LGEGCVIKNCKIHHSVVGLRSCISEGAIIEDTLLMGS 686
            + EGC +    I+ S++   + I EGA+I+D+++M +
Sbjct:  298 IAEGCSVHGTVIN-SILFPGAYIEEGAVIQDSIIMSN 333

HSP 3 Score: 22.7126 bits (47), Expect = 2.742e-21
Identity = 6/11 (54.55%), Postives = 10/11 (90.91%), Query Frame = 3 
Query:   12 DHLYRMDYERL 44
            DH+Y+MDY ++
Sbjct:  128 DHIYKMDYSKM 138
[Close]
BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_STRPS (Glucose-1-phosphate adenylyltransferase OS=Streptococcus pneumoniae (strain CGSP14) GN=glgC PE=3 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 4.628e-21
Identity = 62/184 (33.70%), Postives = 94/184 (51.09%), Query Frame = 2 
Query:   38 EIIQAHRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLNLL---RDKFPGANDFGSEVIPGATSIGMRVQAYLYDGYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKM-LDADVTDSVI 577
            +++Q+H++ +A +TVA L +  K A+ FG+M  D   RI+EF EKP   Q K+ K                   ASMGIY+     + N+L        G +DFG  VIP    +G  V AY + GYW+D+GTIE+ + AN+     P       +R   IY++    PP+ +  +A V DS++
Sbjct:  136 DMLQSHKDNNASLTVAVLDVPLKEASRFGIMNTDANNRIVEFEEKPA--QPKSTK-------------------ASMGIYIFDWQRLRNMLVAAEKSKVGMSDFGKNVIPNYLELGESVYAYEFSGYWKDVGTIESLWEANMEYI-SPENALDSRNRQWKIYSRNLISPPNFLGANAHVEDSLV 297

HSP 2 Score: 24.6386 bits (52), Expect = 4.628e-21
Identity = 10/41 (24.39%), Postives = 23/41 (56.10%), Query Frame = 3 
Query:  582 EGCVIKNCKIHHSVVGLRSCISEGAIIEDTLLMGSRLL*DG 704
            +GC +    + HS++   + + EGA + D+++M   ++  G
Sbjct:  299 DGCFVDGT-VKHSILSTGAQVREGAEVLDSVIMSGAIIGQG 338

HSP 3 Score: 22.3274 bits (46), Expect = 4.628e-21
Identity = 6/13 (46.15%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:   12 DHLYRMDYERLFR 50
            DH+Y+MDY+ + +
Sbjct:  127 DHIYKMDYDDMLQ 139
[Close]
BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_SALAR (Glucose-1-phosphate adenylyltransferase OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) GN=glgC PE=3 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 5.082e-21
Identity = 67/182 (36.81%), Postives = 94/182 (51.65%), Query Frame = 2 
Query:   53 HRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLNLLR--DK-FPGANDFGSEVIPGATSIGMRVQAYLY-------------DGYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKML 550
            H E  A  TVA +P+  K ATAFG+M +DE  +II+F EKP          +   +  DD +A     +ASMGIYV   D +  LL   DK    ++DFG ++IP  T  GM   AY +             + YW D+GT+EA++ ANL +     P+   YD++ PI T    LPP+K +
Sbjct:  156 HVEKGARCTVACMPVPIKEATAFGVMAVDESEKIIDFVEKP---------ANPPAMPGDDSKA-----LASMGIYVFDADYLYELLAADDKDDASSHDFGKDIIPKITREGM---AYAHPFPLSCVQSDPDAEPYWRDVGTLEAYWKANLDLA-SVTPELDMYDQNWPIRTHMESLPPAKFV 319

HSP 2 Score: 24.2534 bits (51), Expect = 5.082e-21
Identity = 10/18 (55.56%), Postives = 12/18 (66.67%), Query Frame = 3 
Query:   12 DHLYRMDYER-LFRHTEK 62
            DH+Y+ DY R L  H EK
Sbjct:  142 DHIYKQDYSRMLIDHVEK 159
[Close]
BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_ECO7I (Glucose-1-phosphate adenylyltransferase OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=glgC PE=3 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 5.082e-21
Identity = 65/183 (35.52%), Postives = 95/183 (51.91%), Query Frame = 2 
Query:   53 HRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLNLL----RDKFPGANDFGSEVIPGATSIGMRVQAYLY-------------DGYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKML 550
            H E  A  TVA +P+  + A+AFG+M +DE  +IIEF EKP                + ++ +K    +ASMGIYV   D +  LL    RDK   ++DFG ++IP  T  G+   AY +             + YW D+GT+EA++ ANL +    +P+   YDR+ PI T    LPP+K +
Sbjct:  156 HVEKGARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPP-----------SMPNDPSKS---LASMGIYVFDADYLYELLEEDDRDK-NSSHDFGKDLIPKITEAGL---AYAHPFPLSCVQSDPDAEPYWRDVGTLEAYWKANLDLA-SVVPELDMYDRNWPIRTYNESLPPAKFV 319

HSP 2 Score: 24.2534 bits (51), Expect = 5.082e-21
Identity = 10/18 (55.56%), Postives = 12/18 (66.67%), Query Frame = 3 
Query:   12 DHLYRMDYER-LFRHTEK 62
            DH+Y+ DY R L  H EK
Sbjct:  142 DHIYKQDYSRMLIDHVEK 159
The following BLAST results are available for this feature:
BLAST of EY728837 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 310
Match NameE-valueIdentityDescription
GLGC_THERP3.426e-2332.18Glucose-1-phosphate adenylyltransferase OS=Thermom... [more]
GLGC_NITOC7.549e-2334.81Glucose-1-phosphate adenylyltransferase OS=Nitroso... [more]
GLGC_ANADF1.216e-2231.82Glucose-1-phosphate adenylyltransferase OS=Anaerom... [more]
GLGC_THET21.233e-2134.05Glucose-1-phosphate adenylyltransferase OS=Thermus... [more]
GLGC_THEAB1.633e-2132.95Glucose-1-phosphate adenylyltransferase OS=Thermos... [more]
GLGC_THEM42.118e-2133.51Glucose-1-phosphate adenylyltransferase OS=Thermos... [more]
GLGC_CLOCE2.742e-2130.27Glucose-1-phosphate adenylyltransferase OS=Clostri... [more]
GLGC_STRPS4.628e-2133.70Glucose-1-phosphate adenylyltransferase OS=Strepto... [more]
GLGC_SALAR5.082e-2136.81Glucose-1-phosphate adenylyltransferase OS=Salmone... [more]
GLGC_ECO7I5.082e-2135.52Glucose-1-phosphate adenylyltransferase OS=Escheri... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-009-C05-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY728837 ID=EY728837; Name=EY728837; organism=Citrus sinensis; type=EST; length=901bp
CCACGCGTCCGGATCATTTGTACCGAATGGACTATGAGAGATTATTCAGG
CACACAGAGAAACTGATGCGGATATCACAGTAGCTGCGCTGCCAATGGAT
GAAAAACGGGCTACTGCTTTTGGTCTGATGAAGATCGATGAAGAGGGACG
CATAATTGAATTTTCTGAGAAACCAAAAGGAGAGCAACTTAAAGCTATGA
AGGTTGATACCACCATATTAGGTCTTGATGATGAGAGAGCTAAAGAGATG
CCTTACATAGCAAGTATGGGTATTTATGTCATCAGCAAAGATGTGATGTT
AAATCTGCTAAGGGATAAATTTCCTGGAGCTAATGATTTTGGAAGTGAAG
TTATCCCTGGTGCAACTTCCATTGGGATGAGAGTGCAAGCATATTTATAT
GATGGCTACTGGGAAGACATTGGAACAATTGAGGCTTTCTACAATGCGAA
TCTGGGGATCACCAAAAAGCCAATTCCGGATTTCAGCTTCTATGACCGTT
CTGCTCCAATCTATACACAGCCTAGATATCTACCTCCTTCTAAGATGCTT
GATGCTGATGTCACTGATAGTGTAATTGGGTGAAGGCTGTGTGATTAAGA
ATTGCAAAATTCACCATTCTGTTGTTGGGCTTCGATCTTGCATATCAGAA
GGTGCAATTATCGAAGATACTTTACTTATGGGGAGCAGATTATTATGAGA
CGGATGCGGACAGGAGGTTTTTGGGCTGCAAGGGTATTGTTCCATTTGGT
ATTGGCAGAATTCTCACATCAAGAGAGCCATAATCCACANNATGCTCGAA
TGGGGGATTATGTGAAAATTCTTCACAGTGACAGTGTGCAAAAACCACCA
GGGAAACAGATGGATACTCATCAAAACCGGGATTGTTCCATAATCAGGAT
G
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