EY728837 | Citrus Genome Database

Overview


Name	EY728837
Unique Name	EY728837
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	901

Libraries


Library Name	Type
Sweet orange fruit, development stadium (5 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_THET8 (Glucose-1-phosphate adenylyltransferase OS=Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) GN=glgC PE=3 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 6.119e-21
Identity = 62/185 (33.51%), Postives = 99/185 (53.51%), Query Frame = 2

Query:   41 IIQAHRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLNLL----RDKFPGANDFGSEVIPGATSIGMRVQAYLY-----------DGYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKML 550
            +++ H +T ADIT+AA P+    AT FG++++DEE RI EF EKP+  +    + D                +ASMG Y+   + +  LL    RD+   A+DFG +VIP A   G RV AY +           + YW D+GT++A+Y A++ +  K +P+F  ++   P+ T   + PP+K +
Sbjct:  141 MVEYHYDTRADITIAAYPVPVAEATRFGVLQVDEEWRITEFQEKPEEPKPIPGRPDMA--------------LASMGNYIFRTEALFELLEADARDE-TSAHDFGKDVIPRALREGYRVYAYDFHRNPIPGQEGPNLYWRDVGTLDAYYEASMDLV-KVVPEFDLFNPEWPLRTANLFSPPAKFV 309

[Close]

BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_EUBE2 (Glucose-1-phosphate adenylyltransferase OS=Eubacterium eligens (strain ATCC 27750 / VPI C15-48) GN=glgC PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 8.602e-21
Identity = 60/182 (32.97%), Postives = 99/182 (54.40%), Query Frame = 2

Query:   41 IIQAHRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLN-LLRDKFPGANDFGSEVIPGATSIGMRVQAYLYDGYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKML-DADVTDSVIG 580
            ++  H+  +ADIT+AA+P+  + A+ FG++  D + +I EF EKP+  +                       +ASMGIY+ S  V+ + L++ K     DFG  VIP   +   R+ AY Y+GYW+D+GT+ +++ AN+ +    IP F+ Y+    IYT+   +PP  +  DA +  S+IG
Sbjct:  138 MLDYHKANNADITIAAMPVPMEEASRFGVVVTDNDNKITEFEEKPEHPKSN---------------------LASMGIYIFSWKVLKDALIKLKDQQECDFGKHVIPYCFNNNKRIFAYEYNGYWKDVGTLSSYWEANMELI-DIIPIFNLYEEFWKIYTKTDTIPPQYIAKDAYIEKSIIG 297

HSP 2 Score: 21.557 bits (44), Expect = 8.602e-21
Identity = 6/9 (66.67%), Postives = 9/9 (100.00%), Query Frame = 3

Query:   12 DHLYRMDYE 38
            DH+Y+MDY+
Sbjct:  128 DHIYKMDYK 136

[Close]

BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC2_ALHEH (Glucose-1-phosphate adenylyltransferase 2 OS=Alkalilimnicola ehrlichei (strain MLHE-1) GN=glgC2 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.004e-20
Identity = 60/182 (32.97%), Postives = 94/182 (51.65%), Query Frame = 2

Query:   41 IIQAHRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLN-LLRD-KFPGANDFGSEVIPGATSIGMRVQAYLY-------DGYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKMLDAD 559
            ++  H ET AD+TV  L +  + A+AFG+M +D + R++ F EKP          D   +    +RA     +ASMGIY+ ++  + N L+ D +    +DFG ++IP       RV A+ +         YW D+GTI+AF+  NL +     P  + YD+  PI+T    LPP+K +  D
Sbjct:  148 MLARHVETGADVTVGCLEVPVEEASAFGVMAVDGDNRVVRFQEKP---------ADPPSIPGQSDRA-----LASMGIYIFNRAFLFNQLIADARKESDHDFGKDIIPSLID-QARVIAFPFRDAATGGQAYWRDVGTIDAFWRTNLELVGVN-PQLNLYDKEWPIWTHQEQLPPAKFVFDD 313

HSP 2 Score: 25.0238 bits (53), Expect = 1.004e-20
Identity = 9/41 (21.95%), Postives = 21/41 (51.22%), Query Frame = 3

Query:  585 GCVIKNCKIHHSVVGLRSCISEGAIIEDTLLMGSRLL*DGC 707
            GC+I    +  S++     + +G+ +ED +++    +  GC
Sbjct:  328 GCIISGAYLRRSLLFSSVVVEDGSRVEDAVILPEAHIEPGC 368

HSP 3 Score: 23.0978 bits (48), Expect = 1.004e-20
Identity = 10/17 (58.82%), Postives = 12/17 (70.59%), Query Frame = 3

Query:   12 DHLYRMDY-ERLFRHTE 59
            DH+Y+MDY   L RH E
Sbjct:  138 DHVYKMDYGPMLARHVE 154

[Close]

BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_RHOPT (Glucose-1-phosphate adenylyltransferase OS=Rhodopseudomonas palustris (strain TIE-1) GN=glgC PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.004e-20
Identity = 62/185 (33.51%), Postives = 89/185 (48.11%), Query Frame = 2

Query:   38 EIIQAHRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLNLLRDKFPGAN---DFGSEVIPGATSIGMRVQAYLYD-----------GYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKML 550
            +++Q H E  AD+TV  L +    ATAFG+M  D   RII F EKP        K D +++              SMGIYV     +L+ LR      N   DFG ++IP     G  V A+ +D            YW D+GT++A++ AN+ +T   +P+   YDR  PI+T     PP+K +
Sbjct:  144 KMLQQHVEQGADVTVGCLEVPRAEATAFGVMHTDTTDRIISFLEKPADPPAMPGKADKSLV--------------SMGIYVFETKFLLDELRRDAADPNSSHDFGKDIIPYIVKHGKAV-AHHFDKSCRRSSSEAVSYWRDVGTVDAYWAANIDLT-DIVPELDLYDREWPIWTYGEITPPAKFV 312

HSP 2 Score: 25.409 bits (54), Expect = 1.004e-20
Identity = 9/18 (50.00%), Postives = 15/18 (83.33%), Query Frame = 3

Query:   12 DHLYRMDYERLF-RHTEK 62
            DH+Y+MDYE++  +H E+
Sbjct:  135 DHVYKMDYEKMLQQHVEQ 152

HSP 3 Score: 20.7866 bits (42), Expect = 1.004e-20
Identity = 7/32 (21.88%), Postives = 18/32 (56.25%), Query Frame = 3

Query:  585 GCVIKNCKIHHSVVGLRSCISEGAIIEDTLLM 680
            GC+I    + HS++     +   + +E+T+++
Sbjct:  330 GCIISGASLRHSLLFTGVRVHSFSHVENTVVL 361

[Close]

BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_RHOPA (Glucose-1-phosphate adenylyltransferase OS=Rhodopseudomonas palustris GN=glgC PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.004e-20
Identity = 62/185 (33.51%), Postives = 89/185 (48.11%), Query Frame = 2

Query:   38 EIIQAHRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLNLLRDKFPGAN---DFGSEVIPGATSIGMRVQAYLYD-----------GYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKML 550
            +++Q H E  AD+TV  L +    ATAFG+M  D   RII F EKP        K D +++              SMGIYV     +L+ LR      N   DFG ++IP     G  V A+ +D            YW D+GT++A++ AN+ +T   +P+   YDR  PI+T     PP+K +
Sbjct:  144 KMLQQHVEQGADVTVGCLEVPRAEATAFGVMHTDTTDRIISFLEKPADPPAMPGKADKSLV--------------SMGIYVFETKFLLDELRRDAADPNSSHDFGKDIIPYIVKHGKAV-AHHFDKSCRRSSSEAVSYWRDVGTVDAYWAANIDLT-DIVPELDLYDREWPIWTYGEITPPAKFV 312

HSP 2 Score: 25.409 bits (54), Expect = 1.004e-20
Identity = 9/18 (50.00%), Postives = 15/18 (83.33%), Query Frame = 3

Query:   12 DHLYRMDYERLF-RHTEK 62
            DH+Y+MDYE++  +H E+
Sbjct:  135 DHVYKMDYEKMLQQHVEQ 152

HSP 3 Score: 20.7866 bits (42), Expect = 1.004e-20
Identity = 7/32 (21.88%), Postives = 18/32 (56.25%), Query Frame = 3

Query:  585 GCVIKNCKIHHSVVGLRSCISEGAIIEDTLLM 680
            GC+I    + HS++     +   + +E+T+++
Sbjct:  330 GCIISGASLRHSLLFTGVRVHSFSHVENTVVL 361

[Close]

BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_STRP7 (Glucose-1-phosphate adenylyltransferase OS=Streptococcus pneumoniae (strain 70585) GN=glgC PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 1.008e-20
Identity = 62/184 (33.70%), Postives = 93/184 (50.54%), Query Frame = 2

Query:   38 EIIQAHRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLNLL---RDKFPGANDFGSEVIPGATSIGMRVQAYLYDGYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKM-LDADVTDSVI 577
            +++Q+H++ +A +TVA L +  K A+ FG+M  D   RI+EF EKP   Q K+ K                   ASMGIY+     + N+L        G +DFG  VIP     G  V AY + GYW+D+GTIE+ + AN+     P       +R   IY++    PP+ +  +A V DS++
Sbjct:  136 DMLQSHKDNNASLTVAVLDVPLKEASRFGIMNTDANNRIVEFEEKPA--QPKSTK-------------------ASMGIYIFDWQRLRNMLVAAEKSKVGMSDFGKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYI-SPENALDSRNRQWKIYSRNLISPPNFLGANAHVEDSLV 297

HSP 2 Score: 25.0238 bits (53), Expect = 1.008e-20
Identity = 11/41 (26.83%), Postives = 23/41 (56.10%), Query Frame = 3

Query:  582 EGCVIKNCKIHHSVVGLRSCISEGAIIEDTLLMGSRLL*DG 704
            +GC +    + HS++   + + EGA I D+++M   ++  G
Sbjct:  299 DGCFVDGT-VKHSILSTGAQVREGAEILDSVIMSGAIIGQG 338

HSP 3 Score: 22.3274 bits (46), Expect = 1.008e-20
Identity = 6/13 (46.15%), Postives = 11/13 (84.62%), Query Frame = 3

Query:   12 DHLYRMDYERLFR 50
            DH+Y+MDY+ + +
Sbjct:  127 DHIYKMDYDDMLQ 139

[Close]

BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_SHISS (Glucose-1-phosphate adenylyltransferase OS=Shigella sonnei (strain Ss046) GN=glgC PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.118e-20
Identity = 63/182 (34.62%), Postives = 92/182 (50.55%), Query Frame = 2

Query:   53 HRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLNLLRDKFPGAN---DFGSEVIPGATSIGMRVQAYLY-------------DGYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKML 550
            H E  A  TVA +P+  + A+AFG+M +DE  +IIEF EKP                + ++ +K    +ASMGIYV   D +  LL +     N   DFG ++IP  T  G+   AY +             + YW D+GT+EA++ ANL +    +P+   YDR+ PI T    LPP+K +
Sbjct:  156 HVEKGARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPP-----------SMPNDPSKS---LASMGIYVFDADYLYELLEEDDRDENSSHDFGKDLIPKITEAGL---AYAHPFPLSCVQSDPDAEPYWRDVGTLEAYWKANLDLA-SVVPELDMYDRNWPIRTYNESLPPAKFV 319

HSP 2 Score: 24.2534 bits (51), Expect = 1.118e-20
Identity = 10/18 (55.56%), Postives = 12/18 (66.67%), Query Frame = 3

Query:   12 DHLYRMDYER-LFRHTEK 62
            DH+Y+ DY R L  H EK
Sbjct:  142 DHIYKQDYSRMLIDHVEK 159

[Close]

BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_SHIFL (Glucose-1-phosphate adenylyltransferase OS=Shigella flexneri GN=glgC PE=3 SV=2)

HSP 1 Score: 97.0561 bits (240), Expect = 1.118e-20
Identity = 63/182 (34.62%), Postives = 92/182 (50.55%), Query Frame = 2

Query:   53 HRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLNLLRDKFPGAN---DFGSEVIPGATSIGMRVQAYLY-------------DGYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKML 550
            H E  A  TVA +P+  + A+AFG+M +DE  +IIEF EKP                + ++ +K    +ASMGIYV   D +  LL +     N   DFG ++IP  T  G+   AY +             + YW D+GT+EA++ ANL +    +P+   YDR+ PI T    LPP+K +
Sbjct:  156 HVEKGARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPP-----------SMPNDPSKS---LASMGIYVFDADYLYELLEEDDRDENSSHDFGKDLIPKITEAGL---AYAHPFPLSCVQSDPDAEPYWRDVGTLEAYWKANLDLA-SVVPELDMYDRNWPIRTYNESLPPAKFV 319

HSP 2 Score: 24.2534 bits (51), Expect = 1.118e-20
Identity = 10/18 (55.56%), Postives = 12/18 (66.67%), Query Frame = 3

Query:   12 DHLYRMDYER-LFRHTEK 62
            DH+Y+ DY R L  H EK
Sbjct:  142 DHIYKQDYSRMLIDHVEK 159

[Close]

BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_SHIF8 (Glucose-1-phosphate adenylyltransferase OS=Shigella flexneri serotype 5b (strain 8401) GN=glgC PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.118e-20
Identity = 63/182 (34.62%), Postives = 92/182 (50.55%), Query Frame = 2

Query:   53 HRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLNLLRDKFPGAN---DFGSEVIPGATSIGMRVQAYLY-------------DGYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKML 550
            H E  A  TVA +P+  + A+AFG+M +DE  +IIEF EKP                + ++ +K    +ASMGIYV   D +  LL +     N   DFG ++IP  T  G+   AY +             + YW D+GT+EA++ ANL +    +P+   YDR+ PI T    LPP+K +
Sbjct:  156 HVEKGARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPP-----------SMPNDPSKS---LASMGIYVFDADYLYELLEEDDRDENSSHDFGKDLIPKITEAGL---AYAHPFPLSCVQSDPDAEPYWRDVGTLEAYWKANLDLA-SVVPELDMYDRNWPIRTYNESLPPAKFV 319

HSP 2 Score: 24.2534 bits (51), Expect = 1.118e-20
Identity = 10/18 (55.56%), Postives = 12/18 (66.67%), Query Frame = 3

Query:   12 DHLYRMDYER-LFRHTEK 62
            DH+Y+ DY R L  H EK
Sbjct:  142 DHIYKQDYSRMLIDHVEK 159

[Close]

BLAST of EY728837 vs. ExPASy Swiss-Prot
Match: GLGC_SHIDS (Glucose-1-phosphate adenylyltransferase OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=glgC PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.118e-20
Identity = 63/182 (34.62%), Postives = 92/182 (50.55%), Query Frame = 2

Query:   53 HRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFSEKPKGEQLKAMKVDTTILGLDDERAKEMPYIASMGIYVISKDVMLNLLRDKFPGAN---DFGSEVIPGATSIGMRVQAYLY-------------DGYWEDIGTIEAFYNANLGITKKPIPDFSFYDRSAPIYTQPRYLPPSKML 550
            H E  A  TVA +P+  + A+AFG+M +DE  +IIEF EKP                + ++ +K    +ASMGIYV   D +  LL +     N   DFG ++IP  T  G+   AY +             + YW D+GT+EA++ ANL +    +P+   YDR+ PI T    LPP+K +
Sbjct:  156 HVEKGARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPP-----------SMPNDPSKS---LASMGIYVFDADYLYELLEEDDRDENSSHDFGKDLIPKITEAGL---AYAHPFPLSCVQSDPDAEPYWRDVGTLEAYWKANLDLA-SVVPELDMYDRNWPIRTYNESLPPAKFV 319

HSP 2 Score: 24.2534 bits (51), Expect = 1.118e-20
Identity = 10/18 (55.56%), Postives = 12/18 (66.67%), Query Frame = 3

Query:   12 DHLYRMDYER-LFRHTEK 62
            DH+Y+ DY R L  H EK
Sbjct:  142 DHIYKQDYSRMLIDHVEK 159

The following BLAST results are available for this feature:

BLAST of EY728837 vs. ExPASy Swiss-Prot

BLAST of EY728837 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 310


Match Name	E-value	Identity	Description
GLGC_THET8	6.119e-21	33.51	Glucose-1-phosphate adenylyltransferase OS=Thermus...	[more]
GLGC_EUBE2	8.602e-21	32.97	Glucose-1-phosphate adenylyltransferase OS=Eubacte...	[more]
GLGC2_ALHEH	1.004e-20	32.97	Glucose-1-phosphate adenylyltransferase 2 OS=Alkal...	[more]
GLGC_RHOPT	1.004e-20	33.51	Glucose-1-phosphate adenylyltransferase OS=Rhodops...	[more]
GLGC_RHOPA	1.004e-20	33.51	Glucose-1-phosphate adenylyltransferase OS=Rhodops...	[more]
GLGC_STRP7	1.008e-20	33.70	Glucose-1-phosphate adenylyltransferase OS=Strepto...	[more]
GLGC_SHISS	1.118e-20	34.62	Glucose-1-phosphate adenylyltransferase OS=Shigell...	[more]
GLGC_SHIFL	1.118e-20	34.62	Glucose-1-phosphate adenylyltransferase OS=Shigell...	[more]
GLGC_SHIF8	1.118e-20	34.62	Glucose-1-phosphate adenylyltransferase OS=Shigell...	[more]
GLGC_SHIDS	1.118e-20	34.62	Glucose-1-phosphate adenylyltransferase OS=Shigell...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-704-009-C05-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY728837 ID=EY728837; Name=EY728837; organism=Citrus sinensis; type=EST; length=901bp
CCACGCGTCCGGATCATTTGTACCGAATGGACTATGAGAGATTATTCAGG
CACACAGAGAAACTGATGCGGATATCACAGTAGCTGCGCTGCCAATGGAT
GAAAAACGGGCTACTGCTTTTGGTCTGATGAAGATCGATGAAGAGGGACG
CATAATTGAATTTTCTGAGAAACCAAAAGGAGAGCAACTTAAAGCTATGA
AGGTTGATACCACCATATTAGGTCTTGATGATGAGAGAGCTAAAGAGATG
CCTTACATAGCAAGTATGGGTATTTATGTCATCAGCAAAGATGTGATGTT
AAATCTGCTAAGGGATAAATTTCCTGGAGCTAATGATTTTGGAAGTGAAG
TTATCCCTGGTGCAACTTCCATTGGGATGAGAGTGCAAGCATATTTATAT
GATGGCTACTGGGAAGACATTGGAACAATTGAGGCTTTCTACAATGCGAA
TCTGGGGATCACCAAAAAGCCAATTCCGGATTTCAGCTTCTATGACCGTT
CTGCTCCAATCTATACACAGCCTAGATATCTACCTCCTTCTAAGATGCTT
GATGCTGATGTCACTGATAGTGTAATTGGGTGAAGGCTGTGTGATTAAGA
ATTGCAAAATTCACCATTCTGTTGTTGGGCTTCGATCTTGCATATCAGAA
GGTGCAATTATCGAAGATACTTTACTTATGGGGAGCAGATTATTATGAGA
CGGATGCGGACAGGAGGTTTTTGGGCTGCAAGGGTATTGTTCCATTTGGT
ATTGGCAGAATTCTCACATCAAGAGAGCCATAATCCACANNATGCTCGAA
TGGGGGATTATGTGAAAATTCTTCACAGTGACAGTGTGCAAAAACCACCA
GGGAAACAGATGGATACTCATCAAAACCGGGATTGTTCCATAATCAGGAT
G