EY728531 | Citrus Genome Database

Overview


Name	EY728531
Unique Name	EY728531
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	904

Libraries


Library Name	Type
Sweet orange fruit, development stadium (5 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

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BLAST of EY728531 vs. ExPASy Swiss-Prot
Match: CLPA_RHOBL (ClpA homolog protein OS=Rhodopseudomonas blastica PE=3 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 8.361e-27
Identity = 64/161 (39.75%), Postives = 101/161 (62.73%), Query Frame = 1

Query:  184 EKAGELRDREMDLKAQISALVDKGKEMSKAETEAGDVG--PVVTEVDIQHIVSAWTGIPVEKVSTDESDRLLKMEETLHKRVIGQDEAVKAISRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAYYFGSEEAMIRLDMSEFMERHTVSKLIGS 660
            + A EL  R ++ +    + +D   E   A+    D     V+   +I+ +V+    IP   VS D+++ L  +E TL + V GQD+A++A+S +I+ AR GL+ P +PI +++F+GPTGVGK+E+AK LAA   G E  ++R DMSE+ME+H VS+LIG+
Sbjct:  409 KSAVELASRYINDRKLPDSAIDVIDEAGAAQHLVSDSKRKKVLGTKEIEAVVAKIARIPPRNVSKDDAETLRDLERTLKRLVFGQDKAIEALSASIKLARAGLREPEKPIGNYLFTGPTGVGKTEVAKQLAA-TLGVE--LLRFDMSEYMEKHAVSRLIGA 566

HSP 2 Score: 31.9574 bits (71), Expect = 8.361e-27
Identity = 18/44 (40.91%), Postives = 24/44 (54.55%), Query Frame = 2

Query:  683 EGGQLTEAVRRRPYTVVLFDEIEKL------ILMSSHDASNLED 796
            +GG LT+ V + P+ V+L DEIEK       IL+   D   L D
Sbjct:  575 QGGMLTDGVDQHPHCVLLLDEIEKAHPDVYNILLQVMDHGKLTD 618

[Close]

BLAST of EY728531 vs. ExPASy Swiss-Prot
Match: CLPA_ECOLI (ATP-dependent Clp protease ATP-binding subunit clpA OS=Escherichia coli (strain K12) GN=clpA PE=1 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 1.627e-24
Identity = 53/119 (44.54%), Postives = 80/119 (67.23%), Query Frame = 1

Query:  304 VTEVDIQHIVSAWTGIPVEKVSTDESDRLLKMEETLHKRVIGQDEAVKAISRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAYYFGSEEAMIRLDMSEFMERHTVSKLIGS 660
            V   DI+ +V+    IP + VS  + D L  + + L   V GQD+A++A++ AI+ AR GL + ++P+ SF+F+GPTGVGK+E+   L+    G E  ++R DMSE+MERHTVS+LIG+
Sbjct:  421 VNVADIESVVARIARIPEKSVSQSDRDTLKNLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQLSK-ALGIE--LLRFDMSEYMERHTVSRLIGA 536

HSP 2 Score: 33.4982 bits (75), Expect = 1.627e-24
Identity = 20/50 (40.00%), Postives = 27/50 (54.00%), Query Frame = 2

Query:  683 EGGQLTEAVRRRPYTVVLFDEIEKL------ILMSSHDASNLEDGRLKTA 814
            +GG LT+AV + P+ V+L DEIEK       IL+   D   L D   + A
Sbjct:  545 QGGLLTDAVIKHPHAVLLLDEIEKAHPDVFNILLQVMDNGTLTDNNGRKA 594

[Close]

BLAST of EY728531 vs. ExPASy Swiss-Prot
Match: CLPA_ECOL6 (ATP-dependent Clp protease ATP-binding subunit clpA OS=Escherichia coli O6 GN=clpA PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 1.627e-24
Identity = 53/119 (44.54%), Postives = 80/119 (67.23%), Query Frame = 1

Query:  304 VTEVDIQHIVSAWTGIPVEKVSTDESDRLLKMEETLHKRVIGQDEAVKAISRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAYYFGSEEAMIRLDMSEFMERHTVSKLIGS 660
            V   DI+ +V+    IP + VS  + D L  + + L   V GQD+A++A++ AI+ AR GL + ++P+ SF+F+GPTGVGK+E+   L+    G E  ++R DMSE+MERHTVS+LIG+
Sbjct:  421 VNVADIESVVARIARIPEKSVSQSDRDTLKNLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQLSK-ALGIE--LLRFDMSEYMERHTVSRLIGA 536

HSP 2 Score: 33.4982 bits (75), Expect = 1.627e-24
Identity = 20/50 (40.00%), Postives = 27/50 (54.00%), Query Frame = 2

Query:  683 EGGQLTEAVRRRPYTVVLFDEIEKL------ILMSSHDASNLEDGRLKTA 814
            +GG LT+AV + P+ V+L DEIEK       IL+   D   L D   + A
Sbjct:  545 QGGLLTDAVIKHPHAVLLLDEIEKAHPDVFNILLQVMDNGTLTDNNGRKA 594

[Close]

BLAST of EY728531 vs. ExPASy Swiss-Prot
Match: CLPA_ECO57 (ATP-dependent Clp protease ATP-binding subunit clpA OS=Escherichia coli O157:H7 GN=clpA PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 1.627e-24
Identity = 53/119 (44.54%), Postives = 80/119 (67.23%), Query Frame = 1

Query:  304 VTEVDIQHIVSAWTGIPVEKVSTDESDRLLKMEETLHKRVIGQDEAVKAISRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAYYFGSEEAMIRLDMSEFMERHTVSKLIGS 660
            V   DI+ +V+    IP + VS  + D L  + + L   V GQD+A++A++ AI+ AR GL + ++P+ SF+F+GPTGVGK+E+   L+    G E  ++R DMSE+MERHTVS+LIG+
Sbjct:  421 VNVADIESVVARIARIPEKSVSQSDRDTLKNLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQLSK-ALGIE--LLRFDMSEYMERHTVSRLIGA 536

HSP 2 Score: 33.4982 bits (75), Expect = 1.627e-24
Identity = 20/50 (40.00%), Postives = 27/50 (54.00%), Query Frame = 2

Query:  683 EGGQLTEAVRRRPYTVVLFDEIEKL------ILMSSHDASNLEDGRLKTA 814
            +GG LT+AV + P+ V+L DEIEK       IL+   D   L D   + A
Sbjct:  545 QGGLLTDAVIKHPHAVLLLDEIEKAHPDVFNILLQVMDNGTLTDNNGRKA 594

[Close]

BLAST of EY728531 vs. ExPASy Swiss-Prot
Match: CLPB_RAT (Caseinolytic peptidase B protein homolog OS=Rattus norvegicus GN=Clpb PE=2 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 6.067e-20
Identity = 42/96 (43.75%), Postives = 60/96 (62.50%), Query Frame = 1

Query:  376 ESDRLLKMEETLHKRVIGQDEAVKAISRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAY-YFGSEEAMIRLDMSEFMERHTVSKLIGS 660
            E  R   +E+ L + +IGQ+ A+  +  AIRR   G  +   P+  F+F G +G+GK+ELAK  A Y +  +++  IRLDMSEF ERH V+K IGS
Sbjct:  302 EERRRFPLEQRLEQHIIGQESAIATVGAAIRRKENGWYDEEHPLV-FLFLGSSGIGKTELAKQTAKYMHKDAKKGFIRLDMSEFQERHEVAKFIGS 396

HSP 2 Score: 40.0466 bits (92), Expect = 6.067e-20
Identity = 23/54 (42.59%), Postives = 32/54 (59.26%), Query Frame = 2

Query:  683 EGGQLTEAVRRRPYTVVLFDEIEK------LILMSSHDASNLEDGRLKTAREDC 826
            EGGQLT+ +++ P  VVLFDE++K       I++   D   L DG+ KT   DC
Sbjct:  405 EGGQLTKKLKQCPNAVVLFDEVDKAHPDVLTIMLQLFDEGRLTDGKGKTI--DC 456

[Close]

BLAST of EY728531 vs. ExPASy Swiss-Prot
Match: CLPB_HUMAN (Caseinolytic peptidase B protein homolog OS=Homo sapiens GN=CLPB PE=1 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 7.883e-20
Identity = 42/96 (43.75%), Postives = 60/96 (62.50%), Query Frame = 1

Query:  376 ESDRLLKMEETLHKRVIGQDEAVKAISRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAY-YFGSEEAMIRLDMSEFMERHTVSKLIGS 660
            E  R   +E+ L + +IGQ+ A+  +  AIRR   G  +   P+  F+F G +G+GK+ELAK  A Y +  +++  IRLDMSEF ERH V+K IGS
Sbjct:  332 EERRRFPLEQRLKEHIIGQESAIATVGAAIRRKENGWYDEEHPLV-FLFLGSSGIGKTELAKQTAKYMHKDAKKGFIRLDMSEFQERHEVAKFIGS 426

HSP 2 Score: 40.0466 bits (92), Expect = 7.883e-20
Identity = 23/54 (42.59%), Postives = 32/54 (59.26%), Query Frame = 2

Query:  683 EGGQLTEAVRRRPYTVVLFDEIEK------LILMSSHDASNLEDGRLKTAREDC 826
            EGGQLT+ +++ P  VVLFDE++K       I++   D   L DG+ KT   DC
Sbjct:  435 EGGQLTKKLKQCPNAVVLFDEVDKAHPDVLTIMLQLFDEGRLTDGKGKTI--DC 486

[Close]

BLAST of EY728531 vs. ExPASy Swiss-Prot
Match: CLPB_MOUSE (Caseinolytic peptidase B protein homolog OS=Mus musculus GN=Clpb PE=1 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 7.890e-20
Identity = 42/96 (43.75%), Postives = 60/96 (62.50%), Query Frame = 1

Query:  376 ESDRLLKMEETLHKRVIGQDEAVKAISRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAY-YFGSEEAMIRLDMSEFMERHTVSKLIGS 660
            E  R   +E+ L + +IGQ+ A+  +  AIRR   G  +   P+  F+F G +G+GK+ELAK  A Y +  +++  IRLDMSEF ERH V+K IGS
Sbjct:  302 EERRRFPLEQRLKEHIIGQESAIATVGAAIRRKENGWYDEEHPLV-FLFLGSSGIGKTELAKQTAKYMHKDAKKGFIRLDMSEFQERHEVAKFIGS 396

HSP 2 Score: 40.0466 bits (92), Expect = 7.890e-20
Identity = 23/54 (42.59%), Postives = 32/54 (59.26%), Query Frame = 2

Query:  683 EGGQLTEAVRRRPYTVVLFDEIEK------LILMSSHDASNLEDGRLKTAREDC 826
            EGGQLT+ +++ P  VVLFDE++K       I++   D   L DG+ KT   DC
Sbjct:  405 EGGQLTKKLKQCPNAVVLFDEVDKAHPDVLTIMLQLFDEGRLTDGKGKTI--DC 456

[Close]

BLAST of EY728531 vs. ExPASy Swiss-Prot
Match: CLPB_BOVIN (Caseinolytic peptidase B protein homolog OS=Bos taurus GN=CLPB PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 7.890e-20
Identity = 42/96 (43.75%), Postives = 60/96 (62.50%), Query Frame = 1

Query:  376 ESDRLLKMEETLHKRVIGQDEAVKAISRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAY-YFGSEEAMIRLDMSEFMERHTVSKLIGS 660
            E  R   +E+ L + +IGQ+ A+  +  AIRR   G  +   P+  F+F G +G+GK+ELAK  A Y +  +++  IRLDMSEF ERH V+K IGS
Sbjct:  302 EERRRFPLEQRLKEHIIGQESAIATVGAAIRRKENGWYDEEHPLV-FLFLGSSGIGKTELAKQTAKYMHKDAKKGFIRLDMSEFQERHEVAKFIGS 396

HSP 2 Score: 40.0466 bits (92), Expect = 7.890e-20
Identity = 23/54 (42.59%), Postives = 32/54 (59.26%), Query Frame = 2

Query:  683 EGGQLTEAVRRRPYTVVLFDEIEK------LILMSSHDASNLEDGRLKTAREDC 826
            EGGQLT+ +++ P  VVLFDE++K       I++   D   L DG+ KT   DC
Sbjct:  405 EGGQLTKKLKQCPNAVVLFDEVDKAHPDVLTIMLQLFDEGRLTDGKGKTI--DC 456

[Close]

BLAST of EY728531 vs. ExPASy Swiss-Prot
Match: AMIB_PSEAE (ATP-dependent protease ATP-binding subunit-like protein amiB OS=Pseudomonas aeruginosa GN=amiB PE=3 SV=2)

HSP 1 Score: 69.707 bits (169), Expect = 2.605e-12
Identity = 32/86 (37.21%), Postives = 51/86 (59.30%), Query Frame = 1

Query:  403 ETLHKRVIGQDEAVKAISRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAYYFGSEEAMIRLDMSEFMERHTVSKLIGS 660
            + L   V+GQD A++A+   ++  R  + +P RP+ S +F GPTGVGK+E+ +ALA    G  E   R+DM+   + H  + L G+
Sbjct:   55 DCLRAEVLGQDPALQAVEDMLKVVRADIADPRRPLFSALFLGPTGVGKTEIVRALARALHGDAEGFCRVDMNTLSQEHYAAALTGA 140

HSP 2 Score: 23.483 bits (49), Expect = 2.605e-12
Identity = 14/44 (31.82%), Postives = 22/44 (50.00%), Query Frame = 2

Query:  728 VVLFDEIEKLILMSSHDASNLEDG---RLKTAREDCXLQNTLLY 850
            +VLFDE+EK      H   N+ D    R+ +       +NTL++
Sbjct:  168 IVLFDELEKASPEVVHALLNVLDNGLLRVASGERTYHFRNTLVF 211

[Close]

BLAST of EY728531 vs. ExPASy Swiss-Prot
Match: ADPR_RHOER (ATP-dependent protease ATP-binding subunit-like protein OS=Rhodococcus erythropolis PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 3.389e-12
Identity = 32/89 (35.96%), Postives = 53/89 (59.55%), Query Frame = 1

Query:  394 KMEETLHKRVIGQDEAVKAISRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAYYFGSEEAMIRLDMSEFMERHTVSKLIGS 660
            ++ + L  +++GQ  AV A+ RAI  A VG  +P RP+A+ +  GPTGVGK+EL + +AA      + + R+DM+   + H  +   G+
Sbjct:   35 RLSKALSSKIVGQQAAVDAVVRAISIAHVGATDPTRPLANILLVGPTGVGKTELVRRVAAELRSGPDDLCRIDMNALAQEHYAASFSGA 123

HSP 2 Score: 22.7126 bits (47), Expect = 3.389e-12
Identity = 10/14 (71.43%), Postives = 12/14 (85.71%), Query Frame = 2

Query:  719 PYT--VVLFDEIEK 754
            PYT  +VLFDE+EK
Sbjct:  146 PYTPGIVLFDEVEK 159

The following BLAST results are available for this feature:

BLAST of EY728531 vs. ExPASy Swiss-Prot

BLAST of EY728531 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 200


Match Name	E-value	Identity	Description
CLPA_RHOBL	8.361e-27	39.75	ClpA homolog protein OS=Rhodopseudomonas blastica ...	[more]
CLPA_ECOLI	1.627e-24	44.54	ATP-dependent Clp protease ATP-binding subunit clp...	[more]
CLPA_ECOL6	1.627e-24	44.54	ATP-dependent Clp protease ATP-binding subunit clp...	[more]
CLPA_ECO57	1.627e-24	44.54	ATP-dependent Clp protease ATP-binding subunit clp...	[more]
CLPB_RAT	6.067e-20	43.75	Caseinolytic peptidase B protein homolog OS=Rattus...	[more]
CLPB_HUMAN	7.883e-20	43.75	Caseinolytic peptidase B protein homolog OS=Homo s...	[more]
CLPB_MOUSE	7.890e-20	43.75	Caseinolytic peptidase B protein homolog OS=Mus mu...	[more]
CLPB_BOVIN	7.890e-20	43.75	Caseinolytic peptidase B protein homolog OS=Bos ta...	[more]
AMIB_PSEAE	2.605e-12	37.21	ATP-dependent protease ATP-binding subunit-like pr...	[more]
ADPR_RHOER	3.389e-12	35.96	ATP-dependent protease ATP-binding subunit-like pr...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-704-005-B05-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY728531 ID=EY728531; Name=EY728531; organism=Citrus sinensis; type=EST; length=904bp
CACGCGTCCGGCAATCTTCTCAGTGACCGTTTTCTCCCTGATAAAGCTAT
TGATTTGATTGATGAAGCTGGTTCTCGGGTTCGTCTTCGTCATGCACAGC
TCCCCGAGGAAGCTAGAGAGCTTGAAAAAGAGCTCAGGCAGATAACTAAG
GAGAAGAATGAGGCTGTTCGTGGCCAAGACTTTGAAAAGGCTGGAGAGTT
ACGTGATAGAGAAATGGACCTTAAGGCTCAGATATCTGCTCTTGTCGATA
AAGGCAAAGAGATGAGCAAGGCAGAGACTGAAGCAGGGGATGTTGGTCCT
GTTGTGACTGAAGTGGACATTCAACACATTGTCTCAGCTTGGACGGGCAT
TCCGGTTGAAAAAGTCTCGACTGATGAATCTGATCGCCTCCTCAAGATGG
AAGAGACCCTTCACAAGAGAGTTATTGGTCAGGACGAAGCTGTCAAAGCT
ATTAGTCGTGCTATCCGGCGTGCACGTGTTGGACTCAAAAACCCCAATCG
ACCAATTGCTAGTTTCATCTTTTCTGGTCCAACTGGTGTCGGGAAGTCTG
AACTGGCCAAAGCACTGGCTGCCTATTACTTTGGCTCAGAGGAGGCCATG
ATCAGGCTTGATATGAGTGAGTTCATGGAAAGACACACTGTNTCCAAGCT
CATTGGTTCACACCTGGTTATGTTGGTTACCTGAGGGTGGTCAGCTGACT
GAGGCTGTTCGCCGTCGTCCTTATACTGTTGTACTCTTTGATGAGATTGA
AAAGCTCATCCTGATGTCTTCACATGATGCTTCAAATCTCGAGGATGGAA
GATTGAAGACAGCAAGGGAAGACTGTANACTTCAGAATACTCTTCTATAT
GACATCAATGTGGACCATGGTATTGAGAAGGGGGGAGCCGCATTGGTTTG
ATTT