EY728523 | Citrus Genome Database

Overview


Name	EY728523
Unique Name	EY728523
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	848

Libraries


Library Name	Type
Sweet orange fruit, development stadium (5 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig11754	contig	Csv1_Contig11754:1..849.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY728523 vs. ExPASy Swiss-Prot
Match: ITK_HUMAN (Tyrosine-protein kinase ITK/TSK OS=Homo sapiens GN=ITK PE=1 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 3.299e-18
Identity = 54/168 (32.14%), Postives = 78/168 (46.43%), Query Frame = 3

Query:   90 YGLQIIKNVDLEELRELGSGTYGTVYHGKWRGSD-VAIKRIKKSCFAGRSSEQERLTKDFWREAHILSNLHHPNVVAFYGVVPDGTGGTLATVTEFMVNGSLKHVXXXXXXXXXXXXXXIIAMDAAFGMEYLHSKNIVHFDLKCENXLVNLRDPQRPICKVGDXGLSR 590
            YG  +I   +L  ++E+GSG +G V+ G W   D VAIK I++    G  SE+     DF  EA ++  L HP +V  YGV  +     +  V EFM +G L                  + +D   GM YL    ++H DL   N LV     +  + KV D G++R
Sbjct:  353 YGKWVIDPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIRE----GAMSEE-----DFIEEAEVMMKLSHPKLVQLYGVCLE--QAPICLVFEFMEHGCLSDYLRTQRGLFAAETLLGMCLDVCEGMAYLEEACVIHRDLAARNCLVG----ENQVIKVSDFGMTR 505

HSP 2 Score: 36.965 bits (84), Expect = 3.299e-18
Identity = 18/47 (38.30%), Postives = 27/47 (57.45%), Query Frame = 2

Query:  599 QYSGIRWCQRNTPWMAPELLNGSSNRVSEKVDVFSFGISMWEILTGG 739
            QY+     +    W +PE+   S +R S K DV+SFG+ MWE+ + G
Sbjct:  511 QYTSSTGTKFPVKWASPEVF--SFSRYSSKSDVWSFGVLMWEVFSEG 555

[Close]

BLAST of EY728523 vs. ExPASy Swiss-Prot
Match: YR826_MIMIV (Putative serine/threonine-protein kinase/receptor R826 OS=Acanthamoeba polyphaga mimivirus GN=MIMI_R826 PE=4 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 4.209e-18
Identity = 48/172 (27.91%), Postives = 76/172 (44.19%), Query Frame = 3

Query:  102 IIKNVDLEELRELGSGTYGTVYHGKWRGSDVAIKRIKKSCFAGRSSEQERLTKDFWREAHILSNLHHPNVVAFYGVVPDGTGGTLATVTEFMVNGSLKHVXXXXXXXXXXXXXXIIAMDAAFGMEYLHSKN--IVHFDLKCENXLVNLRDPQRPICKVGDXGLSRXQRNTLV 611
            +I   +++   ++G G+YG VY GKW+  DVAIK+  K          E        E   L  LHHPN++   G         +  VTE+M  G+L+                 I ++ A G+ YLHS +  I+H D+K  N L++    +    K+ D G +R +    +
Sbjct: 1393 VINYDEIKMGEQIGLGSYGVVYRGKWKNVDVAIKKFIK------QKIDENHLLGIREEIAFLKKLHHPNIITMVGA--SLKKPNICIVTEYMAKGNLRDAMRTCTPKLEWHQKIKILVNIAKGISYLHSFDPPIIHRDIKPSNILID----ENWNVKIADFGFARIKEENAI 1552

HSP 2 Score: 41.5874 bits (96), Expect = 4.209e-18
Identity = 19/32 (59.38%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  638 WMAPELLNGSSNRVSEKVDVFSFGISMWEILT 733
            W APE++   ++   EKVDVFSFGI MWE+LT
Sbjct: 1561 WTAPEIIR--NDIYDEKVDVFSFGIVMWEVLT 1590

HSP 3 Score: 76.6406 bits (187), Expect = 1.202e-17
Identity = 55/163 (33.74%), Postives = 80/163 (49.08%), Query Frame = 3

Query:  117 DLEELRELGSGTYGTVYHGKWRGSDVAIKRIKKSCFAGRSSEQERLTKDFWR----EAHILSNLHHPNVVAFYGVVPDGTGGTLATVTEFMVNGSLKHVXXXXXXXXXXXXXXI-IAMDAAFGMEYLHSKNIVHFDLKCENXLVNLRDPQRPICKVGDXGLSR 590
            +LE   +LG+G +G V+ G WRG++VA+K I         S  + +TKD  R    E  +++ L HPNVV F  +        +  V EFM  GSL  +              + IA  A+ GM +LHS  I H DLK  N L++++       KV D GL++
Sbjct:  785 ELELGEQLGTGAFGEVHKGTWRGTEVAVKMI---------SPDKTITKDIERNFKDEVRVMTTLRHPNVVLF--MAASTKPPKMCIVMEFMALGSLHDLLKNELIPDIPFALKVKIAYQASKGMHFLHSSGITHRDLKSLNLLLDIKWN----VKVSDFGLTK 932

HSP 4 Score: 33.8834 bits (76), Expect = 1.202e-17
Identity = 15/32 (46.88%), Postives = 20/32 (62.50%), Query Frame = 2

Query:  638 WMAPELLNGSSNRVSEKVDVFSFGISMWEILT 733
            W APE+L+          DV+SFGI MWE++T
Sbjct:  951 WTAPEILSEDREVDYILSDVYSFGIIMWELIT 982

[Close]

BLAST of EY728523 vs. ExPASy Swiss-Prot
Match: CSK_RAT (Tyrosine-protein kinase CSK OS=Rattus norvegicus GN=Csk PE=1 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 5.615e-18
Identity = 54/168 (32.14%), Postives = 81/168 (48.21%), Query Frame = 3

Query:  117 DLEELRELGSGTYGTVYHGKWRGSDVAIKRIKKSCFAGRSSEQERLTKDFWREAHILSNLHHPNVVAFYGVVPDGTGGTLATVTEFMVNGSL-KHVXXXXXXXXXXXXXXIIAMDAAFGMEYLHSKNIVHFDLKCENXLVNLRDPQRPICKVGDXGLSRXQRNTLVSG 617
            +L+ L+ +G G +G V  G +RG+ VA+K IK    A          + F  EA +++ L H N+V   GV+ +  GG L  VTE+M  GSL  ++                ++D    MEYL   N VH DL   N LV+    +  + KV D GL++   +T  +G
Sbjct:  194 ELKLLQTIGKGEFGDVMLGDYRGNKVAVKCIKNDATA----------QAFLAEASVMTQLRHSNLVQLLGVIVEEKGG-LYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVS----EDNVAKVSDFGLTKEASSTQDTG 346

HSP 2 Score: 34.2686 bits (77), Expect = 5.615e-18
Identity = 17/34 (50.00%), Postives = 21/34 (61.76%), Query Frame = 2

Query:  638 WMAPELLNGSSNRVSEKVDVFSFGISMWEILTGG 739
            W APE L     + S K DV+SFGI +WEI + G
Sbjct:  352 WTAPEALR--EKKFSTKSDVWSFGILLWEIYSFG 383

[Close]

BLAST of EY728523 vs. ExPASy Swiss-Prot
Match: CSK_HUMAN (Tyrosine-protein kinase CSK OS=Homo sapiens GN=CSK PE=1 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 5.615e-18
Identity = 54/168 (32.14%), Postives = 81/168 (48.21%), Query Frame = 3

Query:  117 DLEELRELGSGTYGTVYHGKWRGSDVAIKRIKKSCFAGRSSEQERLTKDFWREAHILSNLHHPNVVAFYGVVPDGTGGTLATVTEFMVNGSL-KHVXXXXXXXXXXXXXXIIAMDAAFGMEYLHSKNIVHFDLKCENXLVNLRDPQRPICKVGDXGLSRXQRNTLVSG 617
            +L+ L+ +G G +G V  G +RG+ VA+K IK    A          + F  EA +++ L H N+V   GV+ +  GG L  VTE+M  GSL  ++                ++D    MEYL   N VH DL   N LV+    +  + KV D GL++   +T  +G
Sbjct:  194 ELKLLQTIGKGEFGDVMLGDYRGNKVAVKCIKNDATA----------QAFLAEASVMTQLRHSNLVQLLGVIVEEKGG-LYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVS----EDNVAKVSDFGLTKEASSTQDTG 346

HSP 2 Score: 34.2686 bits (77), Expect = 5.615e-18
Identity = 17/34 (50.00%), Postives = 21/34 (61.76%), Query Frame = 2

Query:  638 WMAPELLNGSSNRVSEKVDVFSFGISMWEILTGG 739
            W APE L     + S K DV+SFGI +WEI + G
Sbjct:  352 WTAPEALR--EKKFSTKSDVWSFGILLWEIYSFG 383

[Close]

BLAST of EY728523 vs. ExPASy Swiss-Prot
Match: CSK_BOVIN (Tyrosine-protein kinase CSK OS=Bos taurus GN=CSK PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 5.615e-18
Identity = 60/205 (29.27%), Postives = 91/205 (44.39%), Query Frame = 3

Query:    6 RPQDSKADVQDKDESFSDAMIAEMEASIYGLQIIKNVDLEELRELGSGTYGTVYHGKWRGSDVAIKRIKKSCFAGRSSEQERLTKDFWREAHILSNLHHPNVVAFYGVVPDGTGGTLATVTEFMVNGSL-KHVXXXXXXXXXXXXXXIIAMDAAFGMEYLHSKNIVHFDLKCENXLVNLRDPQRPICKVGDXGLSRXQRNTLVSG 617
            +P+  +  V  +DE F       M+            DL+ L+ +G G +G V  G +RG+ VA+K IK    A          + F  EA +++ L H N+V   GV+ +   G L  VTE+M  GSL  ++                ++D    MEYL   N VH DL   N LV+    +  + KV D GL++   +T  +G
Sbjct:  169 KPKVMEGTVAAQDEFFRSGWALNMK------------DLKLLQTIGKGEFGDVMLGDYRGNKVAVKCIKNDATA----------QAFLAEASVMTQLRHSNLVQLLGVIVEEKSG-LYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVS----EDNVAKVSDFGLTKEASSTQDTG 346

HSP 2 Score: 34.2686 bits (77), Expect = 5.615e-18
Identity = 17/34 (50.00%), Postives = 21/34 (61.76%), Query Frame = 2

Query:  638 WMAPELLNGSSNRVSEKVDVFSFGISMWEILTGG 739
            W APE L     + S K DV+SFGI +WEI + G
Sbjct:  352 WTAPEALR--EKKFSTKSDVWSFGILLWEIYSFG 383

[Close]

BLAST of EY728523 vs. ExPASy Swiss-Prot
Match: M313A_XENLA (Mitogen-activated protein kinase kinase kinase 13-A OS=Xenopus laevis GN=map3k13-A PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 7.187e-18
Identity = 51/173 (29.48%), Postives = 76/173 (43.93%), Query Frame = 3

Query:  117 DLEELRELGSGTYGTVYHGKWRGSDVAIKRIKKSCFAGRSSEQERLTKDFWREAHILSNLHHPNVVAFYGVVPDGTGGTLATVTEFMVNGSLKHVXXXXXXXXXXXXXXIIAMDAAFGMEYLHSKNIVHFDLKCENXLVNLRDPQRPICKVGDXGLSRXQRNTLVSGGVRGTL 635
            ++ EL+ LGSG  G V+ GK+RG +VAIK++++     + ++ + L K           L HPN++AF GV           + E+  +G L  V                +   A GM YLH   I+H DLK  N LV   D      K+ D G S+   +        GT+
Sbjct:  168 EISELQWLGSGAQGAVFLGKFRGEEVAIKKVREQ----KETDIKHLRK-----------LKHPNIIAFKGVCTQAP--CYCLIMEYCAHGQLYEVLRAGRKVSPRLLVD-WSNGIASGMNYLHLHKIIHRDLKSPNVLVTHTD----TVKISDFGTSKELSDKSTKMSFAGTV 318

HSP 2 Score: 45.0542 bits (105), Expect = 7.187e-18
Identity = 19/33 (57.58%), Postives = 26/33 (78.79%), Query Frame = 2

Query:  638 WMAPELLNGSSNRVSEKVDVFSFGISMWEILTG 736
            WMAPE++      VSEKVD++SFG+ +WE+LTG
Sbjct:  320 WMAPEVIRNEP--VSEKVDIWSFGVLLWELLTG 350

[Close]

BLAST of EY728523 vs. ExPASy Swiss-Prot
Match: CSK_MOUSE (Tyrosine-protein kinase CSK OS=Mus musculus GN=Csk PE=1 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 7.301e-18
Identity = 54/168 (32.14%), Postives = 81/168 (48.21%), Query Frame = 3

Query:  117 DLEELRELGSGTYGTVYHGKWRGSDVAIKRIKKSCFAGRSSEQERLTKDFWREAHILSNLHHPNVVAFYGVVPDGTGGTLATVTEFMVNGSL-KHVXXXXXXXXXXXXXXIIAMDAAFGMEYLHSKNIVHFDLKCENXLVNLRDPQRPICKVGDXGLSRXQRNTLVSG 617
            +L+ L+ +G G +G V  G +RG+ VA+K IK    A          + F  EA +++ L H N+V   GV+ +  GG L  VTE+M  GSL  ++                ++D    MEYL   N VH DL   N LV+    +  + KV D GL++   +T  +G
Sbjct:  194 ELKLLQTIGKGEFGDVMLGDYRGNKVAVKCIKNDATA----------QAFLAEASVMTQLRHSNLVQLLGVIVEEKGG-LYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVLEAMEYLEGNNFVHRDLAARNVLVS----EDNVAKVSDFGLTKEASSTQDTG 346

HSP 2 Score: 34.2686 bits (77), Expect = 7.301e-18
Identity = 17/34 (50.00%), Postives = 21/34 (61.76%), Query Frame = 2

Query:  638 WMAPELLNGSSNRVSEKVDVFSFGISMWEILTGG 739
            W APE L     + S K DV+SFGI +WEI + G
Sbjct:  352 WTAPEALR--EKKFSTKSDVWSFGILLWEIYSFG 383

[Close]

BLAST of EY728523 vs. ExPASy Swiss-Prot
Match: CSK_CHICK (Tyrosine-protein kinase CSK OS=Gallus gallus GN=CSK PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 9.492e-18
Identity = 55/168 (32.74%), Postives = 81/168 (48.21%), Query Frame = 3

Query:  117 DLEELRELGSGTYGTVYHGKWRGSDVAIKRIKKSCFAGRSSEQERLTKDFWREAHILSNLHHPNVVAFYGVVPDGTGGTLATVTEFMVNGSL-KHVXXXXXXXXXXXXXXIIAMDAAFGMEYLHSKNIVHFDLKCENXLVNLRDPQRPICKVGDXGLSRXQRNTLVSG 617
            DL+ L+ +G G +G V  G +RG+ VA+K IK    A          + F  EA +++ L H N+V   GV+ +   G L  VTE+M  GSL  ++                ++D    MEYL + N VH DL   N LV+    +  I KV D GL++   +T  +G
Sbjct:  194 DLKLLQIIGKGEFGDVMLGDYRGNKVAVKCIKNDATA----------QAFLAEASVMTQLRHSNLVQLLGVIVEEKSG-LYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEANNFVHRDLAARNVLVS----EDNIAKVSDFGLTKEASSTQDTG 346

HSP 2 Score: 34.2686 bits (77), Expect = 9.492e-18
Identity = 17/34 (50.00%), Postives = 21/34 (61.76%), Query Frame = 2

Query:  638 WMAPELLNGSSNRVSEKVDVFSFGISMWEILTGG 739
            W APE L     + S K DV+SFGI +WEI + G
Sbjct:  352 WTAPEALR--EKKFSTKSDVWSFGILLWEIYSFG 383

[Close]

BLAST of EY728523 vs. ExPASy Swiss-Prot
Match: ABL_DROME (Tyrosine-protein kinase Abl OS=Drosophila melanogaster GN=Abl PE=1 SV=3)

HSP 1 Score: 75.485 bits (184), Expect = 1.203e-17
Identity = 55/169 (32.54%), Postives = 75/169 (44.38%), Query Frame = 3

Query:  105 IKNVDLEELRELGSGTYGTVYHGKWR--GSDVAIKRIKKSCFAGRSSEQERLTKDFWREAHILSNLHHPNVVAFYGVVPDGTGGTLATVTEFMVNGSLKHVXXXXXXXXXXXXXXI-IAMDAAFGMEYLHSKNIVHFDLKCENXLVNLRDPQRPICKVGDXGLSRXQRN 602
            I   D+    +LG G YG VY   W+  G+ VA+K +K+   A          KDF  EA I+  + HPN+V   GV           +TEFM +G+L                 + +A   A GM YL S+N +H DL   N LV        + KV D GL+R  R+
Sbjct:  366 ICRTDIMMKHKLGGGQYGEVYEAVWKRYGNTVAVKTLKEDTMA---------LKDFLEEAAIMKEMKHPNLVQLIGVCTREP--PFYIITEFMSHGNLLDFLRSAGRETLDAVALLYMATQIASGMSYLESRNYIHRDLAARNCLVG----DNKLVKVADFGLARLMRD 519

HSP 2 Score: 35.039 bits (79), Expect = 1.203e-17
Identity = 17/34 (50.00%), Postives = 23/34 (67.65%), Query Frame = 2

Query:  638 WMAPELLNGSSNRVSEKVDVFSFGISMWEILTGG 739
            W APE L  + N+ S K DV++FG+ +WEI T G
Sbjct:  534 WTAPEGL--AYNKFSTKSDVWAFGVLLWEIATYG 565

[Close]

BLAST of EY728523 vs. ExPASy Swiss-Prot
Match: M313B_XENLA (Mitogen-activated protein kinase kinase kinase 13-B OS=Xenopus laevis GN=map3k13-B PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.215e-17
Identity = 51/173 (29.48%), Postives = 75/173 (43.35%), Query Frame = 3

Query:  117 DLEELRELGSGTYGTVYHGKWRGSDVAIKRIKKSCFAGRSSEQERLTKDFWREAHILSNLHHPNVVAFYGVVPDGTGGTLATVTEFMVNGSLKHVXXXXXXXXXXXXXXIIAMDAAFGMEYLHSKNIVHFDLKCENXLVNLRDPQRPICKVGDXGLSRXQRNTLVSGGVRGTL 635
            ++ EL+ LGSG  G V+ GK+RG +VAIK++++     + ++ + L K           L HPN++AF GV           + E+   G L  V                +   A GM YLH   I+H DLK  N LV   D      K+ D G S+   +        GT+
Sbjct:  170 EISELQWLGSGAQGAVFLGKFRGEEVAIKKVREQ----KETDIKHLRK-----------LKHPNIIAFKGVCTQAP--CYCILMEYCAQGQLYEVLRAGRKVTPKLLVE-WSTGIASGMNYLHLHKIIHRDLKSPNVLVTHAD----TVKISDFGTSKELSDKSTKMSFAGTV 320

HSP 2 Score: 45.0542 bits (105), Expect = 1.215e-17
Identity = 19/33 (57.58%), Postives = 26/33 (78.79%), Query Frame = 2

Query:  638 WMAPELLNGSSNRVSEKVDVFSFGISMWEILTG 736
            WMAPE++      VSEKVD++SFG+ +WE+LTG
Sbjct:  322 WMAPEVIRNEP--VSEKVDIWSFGVLLWELLTG 352

The following BLAST results are available for this feature:

BLAST of EY728523 vs. ExPASy Swiss-Prot

BLAST of EY728523 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 403


Match Name	E-value	Identity	Description
ITK_HUMAN	3.299e-18	32.14	Tyrosine-protein kinase ITK/TSK OS=Homo sapiens GN...	[more]
YR826_MIMIV	4.209e-18	27.91	Putative serine/threonine-protein kinase/receptor ...	[more]
CSK_RAT	5.615e-18	32.14	Tyrosine-protein kinase CSK OS=Rattus norvegicus G...	[more]
CSK_HUMAN	5.615e-18	32.14	Tyrosine-protein kinase CSK OS=Homo sapiens GN=CSK...	[more]
CSK_BOVIN	5.615e-18	29.27	Tyrosine-protein kinase CSK OS=Bos taurus GN=CSK P...	[more]
M313A_XENLA	7.187e-18	29.48	Mitogen-activated protein kinase kinase kinase 13-...	[more]
CSK_MOUSE	7.301e-18	32.14	Tyrosine-protein kinase CSK OS=Mus musculus GN=Csk...	[more]
CSK_CHICK	9.492e-18	32.74	Tyrosine-protein kinase CSK OS=Gallus gallus GN=CS...	[more]
ABL_DROME	1.203e-17	32.54	Tyrosine-protein kinase Abl OS=Drosophila melanoga...	[more]
M313B_XENLA	1.215e-17	29.48	Mitogen-activated protein kinase kinase kinase 13-...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-704-005-A09-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY728523 ID=EY728523; Name=EY728523; organism=Citrus sinensis; type=EST; length=848bp
CCACGCGTCCGCAGGATTCTAAAGCTGATGTCCAAGATAAGGATGAATCT
TTCAGTGATGCAATGATAGCTGAAATGGAAGCCAGCATCTATGGCCTGCA
GATTATAAAGAATGTTGACCTTGAAGAACTACGAGAGCTTGGATCTGGTA
CCTATGGAACTGTCTATCATGGAAAATGGCGGGGTTCAGATGTTGCTATA
AAGAGAATAAAAAAGAGCTGCTTTGCTGGGAGATCATCAGAGCAAGAACG
ATTGACCAAAGACTTCTGGAGAGAGGCTCATATTCTCTCAAATCTTCATC
ATCCAAATGTTGTTGCTTTTTATGGAGTAGTCCCAGATGGAACTGGAGGA
ACCTTGGCCACTGTAACTGAATTTATGGTCAATGGGTCACTTAAGCATGT
GCTACTTAAGAAGGATAGATCACTTGATCGTCGTAAAAAGCTTATAATTG
CCATGGATGCAGCTTTTGGCATGGAATACTTGCACTCAAAAAACATTGTC
CACTTTGATCTGAAATGTGAAAATNTGTTAGTCAATCTGAGGGATCCTCA
GCGGCCGATATGCAAGGTTGGGGATNNTGGATTGTCAAGAATNCAGCGCA
ATACTCTGGTATCAGGTGGTGTCAGAGGAACACTCCATGGATGGCACCAG
AACTGCTGAATGGTAGCAGTAACCGGGTTTCCGAAAAGGTTGATGTTTTC
TCTTTTGGGATTTCGATGTGGGAAATCTTGACTGGGGGAGGAACCTTATG
CAAATATGCATTGTGGTGCCCTTATGGGGGGGGATGTGAGGGCACGCTTC
CAACTACTATTCCGAACGTGGTGATCTGAGTGGAGAAATGATGGAACG