EY728468

Overview
NameEY728468
Unique NameEY728468
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length888
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
EY728468_ssr491 microsatellite EY728468_ssr491:491..502. BLAST: Citrus ESTs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY728468 vs. ExPASy Swiss-Prot
Match: PYRE_STRT1 (Orotate phosphoribosyltransferase OS=Streptococcus thermophilus (strain CNRZ 1066) GN=pyrE PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.687e-15
Identity = 48/178 (26.97%), Postives = 85/178 (47.75%), Query Frame = 1 
Query:  100 FKLKSGISSPVYIDLRLIVSYPSLLRQISKTIISSLPST-TKYDLVCGVPYTALPIATCVSVSNDVPMLMRRKEIKDYGTAKAIEGNFKKEEICLIIEDLVTSGASVLETAVPLRAAGLVVTDAVVLIDRE-QGGRENLKENGIELHAMIKLSEMVRVLREKGKLDEETERVVMKFLE 627
            F   SGI SP+Y D R+ +SYP     I    +  +     + +++ G     +P    ++   ++P    R + KD+G    IEG   K E  +++EDL+++G SVL+        G  V   V +   E     +N  E G++L  +   +E+++V + KG +  +  R++ KF E
Sbjct:   25 FTWASGIKSPIYTDNRITLSYPETRTLIENGFVKKIKEEFPEVEVIAGTATAGIPHGAIIADKMNLPFAYIRSKPKDHGAGNQIEGRVVKGEKMVVVEDLISTGGSVLDAVAAAEREGADVIGVVAIFTYELPKAEKNFAEAGVKLVTLSNYTELIKVAKVKGYITADGLRLLKKFKE 202
[Close]
BLAST of EY728468 vs. ExPASy Swiss-Prot
Match: PYRE_LACJO (Orotate phosphoribosyltransferase OS=Lactobacillus johnsonii GN=pyrE PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.687e-15
Identity = 54/182 (29.67%), Postives = 89/182 (48.90%), Query Frame = 1 
Query:  100 FKLKSGISSPVYIDLRLIVSYPSLLRQI----SKTIISSLPSTTKYDLVCGVPYTALPIATCVSVSNDVPMLMRRKEIKDYGTAKAIEGNFKKEEICLIIEDLVTSGASVLETA-VPLRAAGLVVTDAVVLIDREQGGRENLKENGIELHAMIKLSEMVRVLREKGKLDEETERVVMKFLEE 630
            F   SG+ SP+Y DLRL VSYP L   I    S  I +  P  T   ++ GV    +P A  V+    +PM+  R + KD+G  + IEG F + +  ++I+DL+T+G SVL       +  G V   A +        +EN KE  ++   ++   E+++   E G +  +   ++  + E+
Sbjct:   24 FTYASGMLSPIYTDLRLTVSYPDLRDMIASDLSNLIAAEFPQAT---IIGGVATAGIPHAALVAEKLHLPMIYVRPKPKDHGKGRQIEGRFSENDQIVLIDDLITTGGSVLNAVKATEKDGGKVAGVASIFTYYLPDAKENFKEANVKYTPLLSYPELLKKENESGHITSDQYDILKTWHED 202
[Close]
BLAST of EY728468 vs. ExPASy Swiss-Prot
Match: PYRE_STRPC (Orotate phosphoribosyltransferase OS=Streptococcus pyogenes serotype M12 (strain MGAS9429) GN=pyrE PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 2.877e-15
Identity = 55/194 (28.35%), Postives = 94/194 (48.45%), Query Frame = 1 
Query:   64 QLHEISAVKF---GNFKLKSGISSPVYIDLRLIVSYPSLLRQISKTIISSLPST-TKYDLVCGVPYTALPIATCVSVSNDVPMLMRRKEIKDYGTAKAIEGNFKKEEICLIIEDLVTSGASVLETAVPLRAAGLVVTDAVVLIDRE-QGGRENLKENGIELHAMIKLSEMVRVLREKGKLDEETERVVMKFLEE 630
            QL +I AV       F   SGI SP+Y D R+ +SYP     I    + ++ +   + +++ G     +P    ++    +P +  R + KD+G    IEG   K +  +IIEDL+++G SVL+ A      G  V   V +   E     +N KE GI+L  +   +E++ V + +G +  +   ++ KF E+
Sbjct:   10 QLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLSYPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFVYIRSKPKDHGAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASREGADVLGVVAIFTYELPKASQNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKED 203
[Close]
BLAST of EY728468 vs. ExPASy Swiss-Prot
Match: PYRE_STRPB (Orotate phosphoribosyltransferase OS=Streptococcus pyogenes serotype M12 (strain MGAS2096) GN=pyrE PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 2.877e-15
Identity = 55/194 (28.35%), Postives = 94/194 (48.45%), Query Frame = 1 
Query:   64 QLHEISAVKF---GNFKLKSGISSPVYIDLRLIVSYPSLLRQISKTIISSLPST-TKYDLVCGVPYTALPIATCVSVSNDVPMLMRRKEIKDYGTAKAIEGNFKKEEICLIIEDLVTSGASVLETAVPLRAAGLVVTDAVVLIDRE-QGGRENLKENGIELHAMIKLSEMVRVLREKGKLDEETERVVMKFLEE 630
            QL +I AV       F   SGI SP+Y D R+ +SYP     I    + ++ +   + +++ G     +P    ++    +P +  R + KD+G    IEG   K +  +IIEDL+++G SVL+ A      G  V   V +   E     +N KE GI+L  +   +E++ V + +G +  +   ++ KF E+
Sbjct:   10 QLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLSYPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFVYIRSKPKDHGAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASREGADVLGVVAIFTYELPKASQNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKED 203
[Close]
BLAST of EY728468 vs. ExPASy Swiss-Prot
Match: PYRE_SYNY3 (Orotate phosphoribosyltransferase OS=Synechocystis sp. (strain PCC 6803) GN=pyrE PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 3.758e-15
Identity = 58/180 (32.22%), Postives = 90/180 (50.00%), Query Frame = 1 
Query:   58 ILQLHEISAVKFGNFKLKSGISSPVYIDLRLIVSYPSLLRQISKTIISSLPSTTKYDLVCGVPYTALPIATCVSV-----SNDVPMLMRRKEIKDYGTAKAIEG-NFKKEEICLIIEDLVTSGASVLETAVPLRAAGLVVTDAVVLIDREQGGRENLKENGIELHAMIKLSEMVRVLREK 579
            +L L    A K G+F L SG  S  YI+ +L+         I + +++ LP   + + V G+   A PI + VS         V  L+ RKE K +GT   IEG         +++ED+VT+G S +     LR AG  V   + L+DR+QGGRE  +  G+   A+  + ++ +V R+K
Sbjct:   21 LLHLLATHAYKEGDFILSSGQPSTYYINGKLVTLRAEGALAIGRLLLTELPD--QVEAVAGLTLGADPIVSAVSTVSAYEEKPVVALIIRKEAKGHGTKAYIEGPELAPGTKVVVLEDVVTTGKSAMLAVERLRNAGYQVDTVISLVDRQQGGREFYQSQGLTFQALFTIGDIQQVYRQK 198
[Close]
BLAST of EY728468 vs. ExPASy Swiss-Prot
Match: PYRE_STRP1 (Orotate phosphoribosyltransferase OS=Streptococcus pyogenes serotype M1 GN=pyrE PE=1 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 3.758e-15
Identity = 55/194 (28.35%), Postives = 93/194 (47.94%), Query Frame = 1 
Query:   64 QLHEISAVKF---GNFKLKSGISSPVYIDLRLIVSYPSLLRQISKTIISSLPST-TKYDLVCGVPYTALPIATCVSVSNDVPMLMRRKEIKDYGTAKAIEGNFKKEEICLIIEDLVTSGASVLETAVPLRAAGLVVTDAVVLIDRE-QGGRENLKENGIELHAMIKLSEMVRVLREKGKLDEETERVVMKFLEE 630
            QL +I AV       F   SGI SP+Y D R+ +SYP     I    + ++ +   + +++ G     +P    ++    +P    R + KD+G    IEG   K +  +IIEDL+++G SVL+ A      G  V   V +   E     +N KE GI+L  +   +E++ V + +G +  +   ++ KF E+
Sbjct:   10 QLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLSYPKTRDLIENGFVETIKAHFPEVEVIAGTATAGIPHGAIIADKMTLPFAYIRSKPKDHGAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASREGADVLGVVAIFTYELPKASQNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKED 203
[Close]
BLAST of EY728468 vs. ExPASy Swiss-Prot
Match: PYRE_STRTD (Orotate phosphoribosyltransferase OS=Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9) GN=pyrE PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 4.908e-15
Identity = 47/178 (26.40%), Postives = 85/178 (47.75%), Query Frame = 1 
Query:  100 FKLKSGISSPVYIDLRLIVSYPSLLRQISKTIISSLPST-TKYDLVCGVPYTALPIATCVSVSNDVPMLMRRKEIKDYGTAKAIEGNFKKEEICLIIEDLVTSGASVLETAVPLRAAGLVVTDAVVLIDRE-QGGRENLKENGIELHAMIKLSEMVRVLREKGKLDEETERVVMKFLE 627
            F   SGI SP+Y D R+ +SYP     I    +  +     + +++ G     +P    ++   ++P    R + KD+G    IEG   K E  +++EDL+++G SVL+        G  V   V +   E     +N  E G++L  +   +E+++V + KG +  +  +++ KF E
Sbjct:   25 FTWASGIKSPIYTDNRITLSYPETRTLIENGFVKKIKEEFPEVEVIAGTATAGIPHGAIIADKMNLPFAYIRSKPKDHGAGNQIEGRVVKGEKMVVVEDLISTGGSVLDAVAAAEREGADVIGVVAIFTYELPKAEKNFAEAGVKLVTLSNYTELIKVAKVKGYITADGLQLLKKFKE 202
[Close]
BLAST of EY728468 vs. ExPASy Swiss-Prot
Match: PYRE_STRPM (Orotate phosphoribosyltransferase OS=Streptococcus pyogenes serotype M28 GN=pyrE PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 4.908e-15
Identity = 55/194 (28.35%), Postives = 93/194 (47.94%), Query Frame = 1 
Query:   64 QLHEISAVKF---GNFKLKSGISSPVYIDLRLIVSYPSLLRQISKTIISSLPST-TKYDLVCGVPYTALPIATCVSVSNDVPMLMRRKEIKDYGTAKAIEGNFKKEEICLIIEDLVTSGASVLETAVPLRAAGLVVTDAVVLIDRE-QGGRENLKENGIELHAMIKLSEMVRVLREKGKLDEETERVVMKFLEE 630
            QL +I AV       F   SGI SP+Y D R+ +SYP     I    + ++ +   + +++ G     +P    ++    +P    R + KD+G    IEG   K +  +IIEDL+++G SVL+ A      G  V   V +   E     +N KE GI+L  +   +E++ V + +G +  +   ++ KF E+
Sbjct:   10 QLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLSYPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFAYIRSKPKDHGAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASLEGADVLGVVAIFTYELPKASQNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKED 203
[Close]
BLAST of EY728468 vs. ExPASy Swiss-Prot
Match: PYRE_STRPF (Orotate phosphoribosyltransferase OS=Streptococcus pyogenes serotype M4 (strain MGAS10750) GN=pyrE PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 4.908e-15
Identity = 55/194 (28.35%), Postives = 93/194 (47.94%), Query Frame = 1 
Query:   64 QLHEISAVKF---GNFKLKSGISSPVYIDLRLIVSYPSLLRQISKTIISSLPST-TKYDLVCGVPYTALPIATCVSVSNDVPMLMRRKEIKDYGTAKAIEGNFKKEEICLIIEDLVTSGASVLETAVPLRAAGLVVTDAVVLIDRE-QGGRENLKENGIELHAMIKLSEMVRVLREKGKLDEETERVVMKFLEE 630
            QL +I AV       F   SGI SP+Y D R+ +SYP     I    + ++ +   + +++ G     +P    ++    +P    R + KD+G    IEG   K +  +IIEDL+++G SVL+ A      G  V   V +   E     +N KE GI+L  +   +E++ V + +G +  +   ++ KF E+
Sbjct:   10 QLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLSYPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFAYIRSKPKDHGAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASREGADVLGVVAIFTYELPKASQNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKED 203
[Close]
BLAST of EY728468 vs. ExPASy Swiss-Prot
Match: PYRE_STRP8 (Orotate phosphoribosyltransferase OS=Streptococcus pyogenes serotype M18 GN=pyrE PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 4.908e-15
Identity = 55/194 (28.35%), Postives = 93/194 (47.94%), Query Frame = 1 
Query:   64 QLHEISAVKF---GNFKLKSGISSPVYIDLRLIVSYPSLLRQISKTIISSLPST-TKYDLVCGVPYTALPIATCVSVSNDVPMLMRRKEIKDYGTAKAIEGNFKKEEICLIIEDLVTSGASVLETAVPLRAAGLVVTDAVVLIDRE-QGGRENLKENGIELHAMIKLSEMVRVLREKGKLDEETERVVMKFLEE 630
            QL +I AV       F   SGI SP+Y D R+ +SYP     I    + ++ +   + +++ G     +P    ++    +P    R + KD+G    IEG   K +  +IIEDL+++G SVL+ A      G  V   V +   E     +N KE GI+L  +   +E++ V + +G +  +   ++ KF E+
Sbjct:   10 QLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLSYPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFAYIRSKPKDHGAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASREGADVLGVVAIFTYELPKASQNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKED 203
The following BLAST results are available for this feature:
BLAST of EY728468 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 271
Match NameE-valueIdentityDescription
PYRE_STRT11.687e-1526.97Orotate phosphoribosyltransferase OS=Streptococcus... [more]
PYRE_LACJO1.687e-1529.67Orotate phosphoribosyltransferase OS=Lactobacillus... [more]
PYRE_STRPC2.877e-1528.35Orotate phosphoribosyltransferase OS=Streptococcus... [more]
PYRE_STRPB2.877e-1528.35Orotate phosphoribosyltransferase OS=Streptococcus... [more]
PYRE_SYNY33.758e-1532.22Orotate phosphoribosyltransferase OS=Synechocystis... [more]
PYRE_STRP13.758e-1528.35Orotate phosphoribosyltransferase OS=Streptococcus... [more]
PYRE_STRTD4.908e-1526.40Orotate phosphoribosyltransferase OS=Streptococcus... [more]
PYRE_STRPM4.908e-1528.35Orotate phosphoribosyltransferase OS=Streptococcus... [more]
PYRE_STRPF4.908e-1528.35Orotate phosphoribosyltransferase OS=Streptococcus... [more]
PYRE_STRP84.908e-1528.35Orotate phosphoribosyltransferase OS=Streptococcus... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-004-B08-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY728468 ID=EY728468; Name=EY728468; organism=Citrus sinensis; type=EST; length=888bp
CCACGCGTCCGCCCAAAAGGCAGAAACGCAATGTCGTCGCATTCGATGGA
GTCATTGATTCTCCAACTCCACGAGATCTCGGCGGTTAAATTCGGAAACT
TTAAGCTAAAATCCGGCATATCGTCTCCCGTTTACATCGACCTCCGACTT
ATCGTTTCGTACCCTTCCCTCCTCCGCCAAATATCCAAAACCATCATCTC
CTCCCTCCCCTCCACAACCAAATACGACCTCGTGTGCGGCGTTCCGTACA
CCGCCCTCCCAATCGCCACCTGCGTCTCCGTCTCCAACGACGTCCCCATG
CTCATGCGGCGCAAGGAAATCAAAGACTACGGCACCGCCAAGGCCATCGA
AGGCAATTTCAAGAAGGAAGAAATCTGTCTGATCATCGAAGATTTAGTCA
CCAGCGGCGCGTCGGTCCTCGAAACGGCGGTGCCATTGAGAGCGGCGGGG
CTGGTGGTGACGGACGCCGTCGTATTGATCGACAGAGAGCAGGGAGGGAG
GGAGAATCTGAAGGAGAACGGAATTGAGTTGCATGCGATGATAAAGCTGA
GCGAAATGGTTAGGGTTTTGAGGGAGAAAGGGAAGCTTGATGAGGAAACG
GAGAGAGTGGTGATGAAGTTTTTGGAGGAAAANAAGAAAGTGGCGGGTTC
GAATTTAAGTGGGGTTAGGGTTAGGGTTTAGGGTTTGAAGTACGAAAAAA
GGGCGATTTGATGAAGAATGAGACTGGGAAAAAATGTTCAAGTGATGGTG
AAAAANGAAAATAATTGTGTTTGCTGGTGATGTTGCCCTGNTGCTGAGTT
GGTCAATTTGCGAGAAGGTGGGCCAAGATCTGGTGTGGAAACCAGGTGAT
ATTTTGCTGATTCCCCGCGAATTGGTGCAANTTGTGGC
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