EY728463 | Citrus Genome Database

Overview


Name	EY728463
Unique Name	EY728463
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	761

Libraries


Library Name	Type
Sweet orange fruit, development stadium (5 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_SHEB9 (Enolase OS=Shewanella baltica (strain OS195) GN=eno PE=3 SV=1)

HSP 1 Score: 194.512 bits (493), Expect = 4.674e-51
Identity = 111/194 (57.22%), Postives = 129/194 (66.49%), Query Frame = 2

Query:   35 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNK-NLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            I  V  R+I DSRGNPTVE +V    G V  AA PSGASTG  EALELRDG  S YLGKGV  AV+NVN +I  AL GKD T Q  +D  M+  LDGT N     K KLGANAILAVSL   KA A  K IPLY HIAEL+G      +PVP  N++NGG HA + + +QEFM+ PVGA  F+E ++MG EI+H
Sbjct:    4 IINVIGREIMDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRYLGKGVLTAVANVNDLIRTALLGKDATAQAELDQIMI-DLDGTEN-----KDKLGANAILAVSLAAAKAAAAFKGIPLYAHIAELNGTPGQYSMPVPMMNILNGGEHADNNVDIQEFMVQPVGAKTFREALRMGAEIFH 191

HSP 2 Score: 28.8758 bits (63), Expect = 4.674e-51
Identity = 15/35 (42.86%), Postives = 20/35 (57.14%), Query Frame = 3

Query:  654 NVGDEGGFAPHYREDKGGI----ELVHTAGVKAGS 746
            +VGDEGGFAP+   +   +    E V  AG K G+
Sbjct:  205 SVGDEGGFAPNLASNADALAIIKEAVELAGYKLGT 239

[Close]

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_SHEB5 (Enolase OS=Shewanella baltica (strain OS155 / ATCC BAA-1091) GN=eno PE=3 SV=1)

HSP 1 Score: 194.512 bits (493), Expect = 4.674e-51
Identity = 111/194 (57.22%), Postives = 129/194 (66.49%), Query Frame = 2

Query:   35 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNK-NLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            I  V  R+I DSRGNPTVE +V    G V  AA PSGASTG  EALELRDG  S YLGKGV  AV+NVN +I  AL GKD T Q  +D  M+  LDGT N     K KLGANAILAVSL   KA A  K IPLY HIAEL+G      +PVP  N++NGG HA + + +QEFM+ PVGA  F+E ++MG EI+H
Sbjct:    4 IINVIGREIMDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRYLGKGVLTAVANVNDLIRTALLGKDATAQAELDQIMI-DLDGTEN-----KDKLGANAILAVSLAAAKAAAAFKGIPLYAHIAELNGTPGQYSMPVPMMNILNGGEHADNNVDIQEFMVQPVGAKTFREALRMGAEIFH 191

HSP 2 Score: 28.8758 bits (63), Expect = 4.674e-51
Identity = 15/35 (42.86%), Postives = 20/35 (57.14%), Query Frame = 3

Query:  654 NVGDEGGFAPHYREDKGGI----ELVHTAGVKAGS 746
            +VGDEGGFAP+   +   +    E V  AG K G+
Sbjct:  205 SVGDEGGFAPNLASNADALAIIKEAVELAGYKLGT 239

[Close]

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_BACCQ (Enolase OS=Bacillus cereus (strain Q1) GN=eno PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 4.674e-51
Identity = 107/194 (55.15%), Postives = 131/194 (67.53%), Query Frame = 2

Query:   32 TITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            TI  V AR++ DSRGNPTVEV+V T  G   RA VPSGASTG +EA+ELRDG  S YLGKGV  AV+NVN II P + G D T+Q  ID  M+ +LDGT N     K KLGANAIL VS+ V  A A    +PLY+++   +  +   LP P  N+INGGSHA + +  QEFMILPVGA  FKE ++MG E++H
Sbjct:    3 TIIDVYAREVLDSRGNPTVEVEVYTESGAFGRAIVPSGASTGEHEAVELRDGDKSRYLGKGVVNAVNNVNEIIAPEIVGFDVTDQAGIDRAMI-ELDGTPN-----KGKLGANAILGVSMAVAHAAADFVGLPLYRYLGGFNAKQ---LPTPMMNIINGGSHADNNVDFQEFMILPVGAPTFKESIRMGAEVFH 187

HSP 2 Score: 31.187 bits (69), Expect = 4.674e-51
Identity = 14/29 (48.28%), Postives = 20/29 (68.97%), Query Frame = 3

Query:  657 VGDEGGFAPHYREDKGGIELVHTAGVKAG 743
            VGDEGGFAP+   ++  +E++  A  KAG
Sbjct:  202 VGDEGGFAPNLGSNREALEVIIEAIEKAG 230

[Close]

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_BACC7 (Enolase OS=Bacillus cereus (strain AH187) GN=eno PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 4.674e-51
Identity = 107/194 (55.15%), Postives = 131/194 (67.53%), Query Frame = 2

Query:   32 TITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            TI  V AR++ DSRGNPTVEV+V T  G   RA VPSGASTG +EA+ELRDG  S YLGKGV  AV+NVN II P + G D T+Q  ID  M+ +LDGT N     K KLGANAIL VS+ V  A A    +PLY+++   +  +   LP P  N+INGGSHA + +  QEFMILPVGA  FKE ++MG E++H
Sbjct:    3 TIIDVYAREVLDSRGNPTVEVEVYTESGAFGRAIVPSGASTGEHEAVELRDGDKSRYLGKGVVNAVNNVNEIIAPEIVGFDVTDQAGIDRAMI-ELDGTPN-----KGKLGANAILGVSMAVAHAAADFVGLPLYRYLGGFNAKQ---LPTPMMNIINGGSHADNNVDFQEFMILPVGAPTFKESIRMGAEVFH 187

HSP 2 Score: 31.187 bits (69), Expect = 4.674e-51
Identity = 14/29 (48.28%), Postives = 20/29 (68.97%), Query Frame = 3

Query:  657 VGDEGGFAPHYREDKGGIELVHTAGVKAG 743
            VGDEGGFAP+   ++  +E++  A  KAG
Sbjct:  202 VGDEGGFAPNLGSNREALEVIIEAIEKAG 230

[Close]

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_BACC1 (Enolase OS=Bacillus cereus (strain ATCC 10987) GN=eno PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 4.674e-51
Identity = 107/194 (55.15%), Postives = 131/194 (67.53%), Query Frame = 2

Query:   32 TITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            TI  V AR++ DSRGNPTVEV+V T  G   RA VPSGASTG +EA+ELRDG  S YLGKGV  AV+NVN II P + G D T+Q  ID  M+ +LDGT N     K KLGANAIL VS+ V  A A    +PLY+++   +  +   LP P  N+INGGSHA + +  QEFMILPVGA  FKE ++MG E++H
Sbjct:    3 TIIDVYAREVLDSRGNPTVEVEVYTESGAFGRAIVPSGASTGEHEAVELRDGDKSRYLGKGVVNAVNNVNEIIAPEIVGFDVTDQAGIDRAMI-ELDGTPN-----KGKLGANAILGVSMAVAHAAADFVGLPLYRYLGGFNAKQ---LPTPMMNIINGGSHADNNVDFQEFMILPVGAPTFKESIRMGAEVFH 187

HSP 2 Score: 31.187 bits (69), Expect = 4.674e-51
Identity = 14/29 (48.28%), Postives = 20/29 (68.97%), Query Frame = 3

Query:  657 VGDEGGFAPHYREDKGGIELVHTAGVKAG 743
            VGDEGGFAP+   ++  +E++  A  KAG
Sbjct:  202 VGDEGGFAPNLGSNREALEVIIEAIEKAG 230

[Close]

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_CLOTH (Enolase OS=Clostridium thermocellum (strain ATCC 27405 / DSM 1237) GN=eno PE=3 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 4.674e-51
Identity = 105/198 (53.03%), Postives = 132/198 (66.67%), Query Frame = 2

Query:   20 KMAITITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSD-YLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            K  + I +V AR+I DSRGNPTVEV+V    G V RAAVPSGASTG +EA+ELRD   + YLGKGV KAV NVN II P + G +  +Q A+DN M+  LDGT N     K KLGANAIL VSL   KA A    + LY++I  ++      LPVP  N+INGG HA + + +QEFMI+PVGAS F+  ++M  E++H
Sbjct:    2 KQYLEIESVFAREILDSRGNPTVEVEVIAEGGFVGRAAVPSGASTGAFEAVELRDNDKNRYLGKGVQKAVENVNNIIAPEVEGMNVFDQAAVDNLMI-SLDGTPN-----KSKLGANAILGVSLATAKAAAEALGLSLYQYIGGVNAK---TLPVPMMNIINGGKHADNSVNIQEFMIMPVGASSFRHALQMCAEVFH 190

HSP 2 Score: 33.8834 bits (76), Expect = 4.674e-51
Identity = 15/29 (51.72%), Postives = 21/29 (72.41%), Query Frame = 3

Query:  657 VGDEGGFAPHYREDKGGIELVHTAGVKAG 743
            VGDEGGFAP+ + D+  I+++  A  KAG
Sbjct:  205 VGDEGGFAPNLKTDEEAIQVILEAVEKAG 233

[Close]

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_FLAJ1 (Enolase OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) GN=eno PE=3 SV=1)

HSP 1 Score: 198.749 bits (504), Expect = 4.674e-51
Identity = 109/192 (56.77%), Postives = 129/192 (67.19%), Query Frame = 2

Query:   35 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            I  V ARQI DSRGNPT+EVDV T +G + RAAVPSGASTG +EA+ELRDGG  YLGKGV  AV+NVN +I   L G    EQ  ID  M+  LDGT N     K KLGANAIL VSL   KA A+   +PLY+++  +S N    LPVP  N+INGGSH+   +A QEFMI PV A+ F   M+MG EI+H
Sbjct:    4 IIKVHARQILDSRGNPTIEVDVVTENGVLGRAAVPSGASTGEHEAVELRDGGKAYLGKGVLNAVNNVNTVIAEELVGTSVFEQNTIDQLMI-DLDGTPN-----KSKLGANAILGVSLAAAKAAANELGLPLYRYVGGVSAN---TLPVPMMNIINGGSHSDAPIAFQEFMIFPVKATSFTHAMQMGTEIFH 186

HSP 2 Score: 24.6386 bits (52), Expect = 4.674e-51
Identity = 13/30 (43.33%), Postives = 18/30 (60.00%), Query Frame = 3

Query:  657 VGDEGGFAPHYR-EDKGGIELVHTAGVKAG 743
            VGDEGGFAP+     +  ++ +  A  KAG
Sbjct:  201 VGDEGGFAPNLAGGTEDALDTIKLAVEKAG 230

[Close]

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_ALTMD (Enolase OS=Alteromonas macleodii (strain DSM 17117 / Deep ecotype) GN=eno PE=3 SV=1)

HSP 1 Score: 196.823 bits (499), Expect = 4.674e-51
Identity = 110/194 (56.70%), Postives = 132/194 (68.04%), Query Frame = 2

Query:   35 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNK-NLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            I+ +  R+I DSRGNPTVE DV    G + RAA PSGASTG  EALELRDG  S YLGKGV+KAV+ +N  I PAL GKD   Q  ID  M+  LDGT N     K+ LGANAILAVSL V KA A  K + LY+HIA+L+G      +PVP  N+INGG HA + + +QEFM+ PVGA  FKE ++MG EI+H
Sbjct:    4 ISRIIGREILDSRGNPTVEADVYLESGAMGRAAAPSGASTGSREALELRDGDKSRYLGKGVTKAVAAINDTISPALLGKDALAQADIDGIMI-DLDGTEN-----KETLGANAILAVSLAVAKAAAAEKGVALYEHIADLNGTSGQYSMPVPMMNIINGGEHADNNVDIQEFMVQPVGAKSFKEALRMGAEIFH 191

HSP 2 Score: 26.5646 bits (57), Expect = 4.674e-51
Identity = 14/31 (45.16%), Postives = 17/31 (54.84%), Query Frame = 3

Query:  657 VGDEGGFAPHYREDKGG----IELVHTAGVK 737
            VGDEGGFAP+   +       +E V  AG K
Sbjct:  206 VGDEGGFAPNLSSNAEALAVIVEAVENAGYK 236

[Close]

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_PSE14 (Enolase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=eno PE=3 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 4.674e-51
Identity = 106/194 (54.64%), Postives = 130/194 (67.01%), Query Frame = 2

Query:   35 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLV-LPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            I  +K R++ DSRGNPTVE DV   +G V  A  PSGASTG  EALELRDG  S Y+GKGV KAV+N+N  I   L GKDP +Q A+D+ M+ +LDGT N     K  LGANAILAVSL   KA A  + +PLY HIA L+G   +  +PVP  N+INGG HA + + +QEFMI PVGA  F E ++ G EI+H
Sbjct:    4 IVDIKGREVLDSRGNPTVEADVVLDNGIVGSACAPSGASTGSREALELRDGDKSRYMGKGVLKAVANINGPIRDLLLGKDPVDQKALDHAMI-KLDGTEN-----KASLGANAILAVSLAAAKAAAQDQDLPLYAHIANLNGTPGVYSMPVPMMNIINGGEHADNNIDIQEFMIQPVGAKTFAEGLRWGTEIFH 191

HSP 2 Score: 30.4166 bits (67), Expect = 4.674e-51
Identity = 13/29 (44.83%), Postives = 18/29 (62.07%), Query Frame = 3

Query:  657 VGDEGGFAPHYREDKGGIELVHTAGVKAG 743
            VGDEGGFAP+   +K  ++ +  A   AG
Sbjct:  206 VGDEGGFAPNLASNKEALDAIAEAVANAG 234

[Close]

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_BRUSU (Enolase OS=Brucella suis GN=eno PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 4.675e-51
Identity = 105/192 (54.69%), Postives = 127/192 (66.15%), Query Frame = 2

Query:   35 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            I  +  R+I DSRGNPTVEVDV   DG   RAAVPSGASTG +EA+ELRDGGS YLGKGV KAV  VN  I  A+AG D   Q  ID  ++  LDG+ N     K  LGANAIL VSL V KA A    +PLY+++    G    VLPVP  N+INGG+HA + +  QEFMILPVGA+  +E ++ G E++H
Sbjct:    4 IIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLGKGVEKAVEVVNGKIFDAIAGMDAESQLLIDQTLI-DLDGSAN-----KGNLGANAILGVSLAVAKAAAQASGLPLYRYV---GGTNAHVLPVPMMNIINGGAHADNPIDFQEFMILPVGATSIREAVRYGSEVFH 186

HSP 2 Score: 31.9574 bits (71), Expect = 4.675e-51
Identity = 13/30 (43.33%), Postives = 20/30 (66.67%), Query Frame = 3

Query:  654 NVGDEGGFAPHYREDKGGIELVHTAGVKAG 743
            NVGDEGGFAP+ +  +  ++ +  +  KAG
Sbjct:  200 NVGDEGGFAPNLKNAQAALDFIMESIEKAG 229

The following BLAST results are available for this feature:

BLAST of EY728463 vs. ExPASy Swiss-Prot

BLAST of EY728463 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
ENO_SHEB9	4.674e-51	57.22	Enolase OS=Shewanella baltica (strain OS195) GN=en...	[more]
ENO_SHEB5	4.674e-51	57.22	Enolase OS=Shewanella baltica (strain OS155 / ATCC...	[more]
ENO_BACCQ	4.674e-51	55.15	Enolase OS=Bacillus cereus (strain Q1) GN=eno PE=3...	[more]
ENO_BACC7	4.674e-51	55.15	Enolase OS=Bacillus cereus (strain AH187) GN=eno P...	[more]
ENO_BACC1	4.674e-51	55.15	Enolase OS=Bacillus cereus (strain ATCC 10987) GN=...	[more]
ENO_CLOTH	4.674e-51	53.03	Enolase OS=Clostridium thermocellum (strain ATCC 2...	[more]
ENO_FLAJ1	4.674e-51	56.77	Enolase OS=Flavobacterium johnsoniae (strain ATCC ...	[more]
ENO_ALTMD	4.674e-51	56.70	Enolase OS=Alteromonas macleodii (strain DSM 17117...	[more]
ENO_PSE14	4.674e-51	54.64	Enolase OS=Pseudomonas syringae pv. phaseolicola (...	[more]
ENO_BRUSU	4.675e-51	54.69	Enolase OS=Brucella suis GN=eno PE=3 SV=1	[more]

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Properties


Property Name	Value
Genbank description	CS00-C3-704-004-B01-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY728463 ID=EY728463; Name=EY728463; organism=Citrus sinensis; type=EST; length=761bp
CCACGCGTCCGGTTTTCAGAAAATGGCAATAACCATCACTGCCGTCAAAG
CCAGGCAGATCTTTGACAGCCGTGGAAATCCAACGGTGGAGGTCGACGTG
ACAACATCAGATGGCCACGTGGCTAGAGCTGCTGTTCCAAGCGGTGCATC
CACCGGAATTTATGAGGCGCTTGAACTAAGAGACGGAGGCTCTGATTACC
TAGGCAAGGGTGTATCGAAAGCTGTTAGCAATGTCAACGCCATCATTGGC
CCTGCACTCGCTGGCAAGGACCCAACTGAGCAGACTGCTATTGACAACTA
TATGGTACAACAACTTGATGGAACTGTAAATGAGTGGGGTTGGTGCAAAC
AGAAGCTTGGAGCAAACGCCATTTTGGCTGTCTCCCTANCTGTCTGCAAG
GCTGGTGCACATGTCAAGAAGATTCCTCTTTACAAGCATATTGCCGAACT
CTCTGGTAACAAGAATTTGGTGTTGCCAGTGCCAGCATTCAATGTCATTA
ATGGTGGATCACATGCTGGAGACAAACTTGCTATGCAGGAGTTTATGATT
CTTCCTGTAGGAGCTTCCTGTTTCAAAGAACCCATGAAGATGGGTGTAGA
GATATATCACCTCTGAAGGTGTGATTAAAAGAGAAATACGGCCGGGATGC
TACAATGTTGGGGATGAAGGGGGGTTTGCGCCCCATTATCGGGAGGACAA
GGGAGGGATTGAGTTGGTCCACACAGCCGGGGTTAAAGCCGGTTCACCGA
GGCAAAGTGGC