EY728463 | Citrus Genome Database

Overview


Name	EY728463
Unique Name	EY728463
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	761

Libraries


Library Name	Type
Sweet orange fruit, development stadium (5 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_BRUSI (Enolase OS=Brucella suis (strain ATCC 23445 / NCTC 10510) GN=eno PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 4.675e-51
Identity = 105/192 (54.69%), Postives = 127/192 (66.15%), Query Frame = 2

Query:   35 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            I  +  R+I DSRGNPTVEVDV   DG   RAAVPSGASTG +EA+ELRDGGS YLGKGV KAV  VN  I  A+AG D   Q  ID  ++  LDG+ N     K  LGANAIL VSL V KA A    +PLY+++    G    VLPVP  N+INGG+HA + +  QEFMILPVGA+  +E ++ G E++H
Sbjct:    4 IIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLGKGVEKAVEVVNGKIFDAIAGMDAESQLLIDQTLI-DLDGSAN-----KGNLGANAILGVSLAVAKAAAQASGLPLYRYV---GGTNAHVLPVPMMNIINGGAHADNPIDFQEFMILPVGATSIREAVRYGSEVFH 186

HSP 2 Score: 31.9574 bits (71), Expect = 4.675e-51
Identity = 13/30 (43.33%), Postives = 20/30 (66.67%), Query Frame = 3

Query:  654 NVGDEGGFAPHYREDKGGIELVHTAGVKAG 743
            NVGDEGGFAP+ +  +  ++ +  +  KAG
Sbjct:  200 NVGDEGGFAPNLKNAQAALDFIMESIEKAG 229

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_BRUO2 (Enolase OS=Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512) GN=eno PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 4.675e-51
Identity = 105/192 (54.69%), Postives = 127/192 (66.15%), Query Frame = 2

Query:   35 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            I  +  R+I DSRGNPTVEVDV   DG   RAAVPSGASTG +EA+ELRDGGS YLGKGV KAV  VN  I  A+AG D   Q  ID  ++  LDG+ N     K  LGANAIL VSL V KA A    +PLY+++    G    VLPVP  N+INGG+HA + +  QEFMILPVGA+  +E ++ G E++H
Sbjct:    4 IIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLGKGVEKAVEVVNGKIFDAIAGMDAENQLLIDQTLI-DLDGSAN-----KGNLGANAILGVSLAVAKAAAQASGLPLYRYV---GGTNAHVLPVPMMNIINGGAHADNPIDFQEFMILPVGATSIREAVRYGSEVFH 186

HSP 2 Score: 31.9574 bits (71), Expect = 4.675e-51
Identity = 13/30 (43.33%), Postives = 20/30 (66.67%), Query Frame = 3

Query:  654 NVGDEGGFAPHYREDKGGIELVHTAGVKAG 743
            NVGDEGGFAP+ +  +  ++ +  +  KAG
Sbjct:  200 NVGDEGGFAPNLKNAQAALDFIMESIEKAG 229

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_BRUME (Enolase OS=Brucella melitensis GN=eno PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 4.675e-51
Identity = 105/192 (54.69%), Postives = 127/192 (66.15%), Query Frame = 2

Query:   35 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            I  +  R+I DSRGNPTVEVDV   DG   RAAVPSGASTG +EA+ELRDGGS YLGKGV KAV  VN  I  A+AG D   Q  ID  ++  LDG+ N     K  LGANAIL VSL V KA A    +PLY+++    G    VLPVP  N+INGG+HA + +  QEFMILPVGA+  +E ++ G E++H
Sbjct:    4 IIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLGKGVEKAVEVVNGKIFDAIAGMDAESQLLIDQTLI-DLDGSAN-----KGNLGANAILGVSLAVAKAAAQASGLPLYRYV---GGTNAHVLPVPMMNIINGGAHADNPIDFQEFMILPVGATSIREAVRYGSEVFH 186

HSP 2 Score: 31.9574 bits (71), Expect = 4.675e-51
Identity = 13/30 (43.33%), Postives = 20/30 (66.67%), Query Frame = 3

Query:  654 NVGDEGGFAPHYREDKGGIELVHTAGVKAG 743
            NVGDEGGFAP+ +  +  ++ +  +  KAG
Sbjct:  200 NVGDEGGFAPNLKNAQAALDFIMESIEKAG 229

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_BRUMB (Enolase OS=Brucella melitensis biotype 2 (strain ATCC 23457) GN=eno PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 4.675e-51
Identity = 105/192 (54.69%), Postives = 127/192 (66.15%), Query Frame = 2

Query:   35 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            I  +  R+I DSRGNPTVEVDV   DG   RAAVPSGASTG +EA+ELRDGGS YLGKGV KAV  VN  I  A+AG D   Q  ID  ++  LDG+ N     K  LGANAIL VSL V KA A    +PLY+++    G    VLPVP  N+INGG+HA + +  QEFMILPVGA+  +E ++ G E++H
Sbjct:    4 IIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLGKGVEKAVEVVNGKIFDAIAGMDAESQLLIDQTLI-DLDGSAN-----KGNLGANAILGVSLAVAKAAAQASGLPLYRYV---GGTNAHVLPVPMMNIINGGAHADNPIDFQEFMILPVGATSIREAVRYGSEVFH 186

HSP 2 Score: 31.9574 bits (71), Expect = 4.675e-51
Identity = 13/30 (43.33%), Postives = 20/30 (66.67%), Query Frame = 3

Query:  654 NVGDEGGFAPHYREDKGGIELVHTAGVKAG 743
            NVGDEGGFAP+ +  +  ++ +  +  KAG
Sbjct:  200 NVGDEGGFAPNLKNAQAALDFIMESIEKAG 229

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_BRUC2 (Enolase OS=Brucella canis (strain ATCC 23365 / NCTC 10854) GN=eno PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 4.675e-51
Identity = 105/192 (54.69%), Postives = 127/192 (66.15%), Query Frame = 2

Query:   35 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            I  +  R+I DSRGNPTVEVDV   DG   RAAVPSGASTG +EA+ELRDGGS YLGKGV KAV  VN  I  A+AG D   Q  ID  ++  LDG+ N     K  LGANAIL VSL V KA A    +PLY+++    G    VLPVP  N+INGG+HA + +  QEFMILPVGA+  +E ++ G E++H
Sbjct:    4 IIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLGKGVEKAVEVVNGKIFDAIAGMDAESQLLIDQTLI-DLDGSAN-----KGNLGANAILGVSLAVAKAAAQASGLPLYRYV---GGTNAHVLPVPMMNIINGGAHADNPIDFQEFMILPVGATSIREAVRYGSEVFH 186

HSP 2 Score: 31.9574 bits (71), Expect = 4.675e-51
Identity = 13/30 (43.33%), Postives = 20/30 (66.67%), Query Frame = 3

Query:  654 NVGDEGGFAPHYREDKGGIELVHTAGVKAG 743
            NVGDEGGFAP+ +  +  ++ +  +  KAG
Sbjct:  200 NVGDEGGFAPNLKNAQAALDFIMESIEKAG 229

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_BRUAB (Enolase OS=Brucella abortus GN=eno PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 4.675e-51
Identity = 105/192 (54.69%), Postives = 127/192 (66.15%), Query Frame = 2

Query:   35 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            I  +  R+I DSRGNPTVEVDV   DG   RAAVPSGASTG +EA+ELRDGGS YLGKGV KAV  VN  I  A+AG D   Q  ID  ++  LDG+ N     K  LGANAIL VSL V KA A    +PLY+++    G    VLPVP  N+INGG+HA + +  QEFMILPVGA+  +E ++ G E++H
Sbjct:    4 IIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLGKGVEKAVEVVNGKIFDAIAGMDAESQLLIDQTLI-DLDGSAN-----KGNLGANAILGVSLAVAKAAAQASGLPLYRYV---GGTNAHVLPVPMMNIINGGAHADNPIDFQEFMILPVGATSIREAVRYGSEVFH 186

HSP 2 Score: 31.9574 bits (71), Expect = 4.675e-51
Identity = 13/30 (43.33%), Postives = 20/30 (66.67%), Query Frame = 3

Query:  654 NVGDEGGFAPHYREDKGGIELVHTAGVKAG 743
            NVGDEGGFAP+ +  +  ++ +  +  KAG
Sbjct:  200 NVGDEGGFAPNLKNAQAALDFIMESIEKAG 229

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_BRUA2 (Enolase OS=Brucella abortus (strain 2308) GN=eno PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 4.675e-51
Identity = 105/192 (54.69%), Postives = 127/192 (66.15%), Query Frame = 2

Query:   35 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            I  +  R+I DSRGNPTVEVDV   DG   RAAVPSGASTG +EA+ELRDGGS YLGKGV KAV  VN  I  A+AG D   Q  ID  ++  LDG+ N     K  LGANAIL VSL V KA A    +PLY+++    G    VLPVP  N+INGG+HA + +  QEFMILPVGA+  +E ++ G E++H
Sbjct:    4 IIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLGKGVEKAVEVVNGKIFDAIAGMDAESQLLIDQTLI-DLDGSAN-----KGNLGANAILGVSLAVAKAAAQASGLPLYRYV---GGTNAHVLPVPMMNIINGGAHADNPIDFQEFMILPVGATSIREAVRYGSEVFH 186

HSP 2 Score: 31.9574 bits (71), Expect = 4.675e-51
Identity = 13/30 (43.33%), Postives = 20/30 (66.67%), Query Frame = 3

Query:  654 NVGDEGGFAPHYREDKGGIELVHTAGVKAG 743
            NVGDEGGFAP+ +  +  ++ +  +  KAG
Sbjct:  200 NVGDEGGFAPNLKNAQAALDFIMESIEKAG 229

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_BRUA1 (Enolase OS=Brucella abortus (strain S19) GN=eno PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 4.675e-51
Identity = 105/192 (54.69%), Postives = 127/192 (66.15%), Query Frame = 2

Query:   35 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            I  +  R+I DSRGNPTVEVDV   DG   RAAVPSGASTG +EA+ELRDGGS YLGKGV KAV  VN  I  A+AG D   Q  ID  ++  LDG+ N     K  LGANAIL VSL V KA A    +PLY+++    G    VLPVP  N+INGG+HA + +  QEFMILPVGA+  +E ++ G E++H
Sbjct:    4 IIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLGKGVEKAVEVVNGKIFDAIAGMDAESQLLIDQTLI-DLDGSAN-----KGNLGANAILGVSLAVAKAAAQASGLPLYRYV---GGTNAHVLPVPMMNIINGGAHADNPIDFQEFMILPVGATSIREAVRYGSEVFH 186

HSP 2 Score: 31.9574 bits (71), Expect = 4.675e-51
Identity = 13/30 (43.33%), Postives = 20/30 (66.67%), Query Frame = 3

Query:  654 NVGDEGGFAPHYREDKGGIELVHTAGVKAG 743
            NVGDEGGFAP+ +  +  ++ +  +  KAG
Sbjct:  200 NVGDEGGFAPNLKNAQAALDFIMESIEKAG 229

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_ALKMQ (Enolase OS=Alkaliphilus metalliredigens (strain QYMF) GN=eno PE=3 SV=1)

HSP 1 Score: 194.512 bits (493), Expect = 6.093e-51
Identity = 105/193 (54.40%), Postives = 133/193 (68.91%), Query Frame = 2

Query:   35 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSD-YLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            I+ V AR++ DSRGNPT+EV+V    G + RA VPSGASTG +EA+ELRDG    YLGKGV KAV NVN II P + G D  +Q AID  M+  LDGT N     K KLGANAIL VS+ V KA A    IPL++++  ++  +   LPVP  N++NGGSHA + + +QEFM++PVGA  FKE ++MG EIYH
Sbjct:    4 ISDVYAREVLDSRGNPTIEVEVYLESGVLGRAIVPSGASTGAFEAVELRDGDKGRYLGKGVLKAVENVNDIIAPEITGLDALDQVAIDKIMLD-LDGTPN-----KAKLGANAILGVSMAVAKAAAEALDIPLFQYLGGVNAKQ---LPVPMMNILNGGSHADNNVDIQEFMVMPVGAKTFKEALRMGTEIYH 187

HSP 2 Score: 28.4906 bits (62), Expect = 6.093e-51
Identity = 12/29 (41.38%), Postives = 19/29 (65.52%), Query Frame = 3

Query:  657 VGDEGGFAPHYREDKGGIELVHTAGVKAG 743
            VGDEGGFAP+   ++  ++++  A   AG
Sbjct:  202 VGDEGGFAPNLSSNEEALQIIMEAIEAAG 230

BLAST of EY728463 vs. ExPASy Swiss-Prot
Match: ENO_THEAB (Enolase OS=Thermosipho africanus (strain TCF52B) GN=eno PE=3 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 6.093e-51
Identity = 109/197 (55.33%), Postives = 134/197 (68.02%), Query Frame = 2

Query:   23 MAITITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSD-YLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLXVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGDKLAMQEFMILPVGASCFKEPMKMGVEIYH 610
            M I I  V AR++ DSRGNPTVEV+V   DG + RA VPSGASTG +EALELRDG    YLGKGV KAV NVN +I P L G +  +Q  IDN ++ +LDGT N     K KLGANAIL VS+ V +A A+  ++PLYK+   L G    VLPVP  NVINGG+HA + L +QEFMI+P GA  F+E ++ G E +H
Sbjct:    1 MYIEIIDVFAREVLDSRGNPTVEVEVMLEDGSIGRAIVPSGASTGKFEALELRDGDKKRYLGKGVLKAVENVNELIAPKLLGLNAYDQVYIDNVLL-ELDGTEN-----KSKLGANAILGVSMAVARAAANSLQLPLYKY---LGGVNAKVLPVPLMNVINGGAHADNNLDIQEFMIVPAGAPSFREALRYGAETFH 188

HSP 2 Score: 29.261 bits (64), Expect = 6.093e-51
Identity = 13/29 (44.83%), Postives = 20/29 (68.97%), Query Frame = 3

Query:  657 VGDEGGFAPHYREDKGGIELVHTAGVKAG 743
            VGDEGGFAP+ + ++  I+++  A   AG
Sbjct:  203 VGDEGGFAPNLKNNEEAIQVLIQAIQSAG 231

The following BLAST results are available for this feature:

BLAST of EY728463 vs. ExPASy Swiss-Prot

BLAST of EY728463 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
ENO_BRUSI	4.675e-51	54.69	Enolase OS=Brucella suis (strain ATCC 23445 / NCTC...	[more]
ENO_BRUO2	4.675e-51	54.69	Enolase OS=Brucella ovis (strain ATCC 25840 / 63/2...	[more]
ENO_BRUME	4.675e-51	54.69	Enolase OS=Brucella melitensis GN=eno PE=3 SV=1	[more]
ENO_BRUMB	4.675e-51	54.69	Enolase OS=Brucella melitensis biotype 2 (strain A...	[more]
ENO_BRUC2	4.675e-51	54.69	Enolase OS=Brucella canis (strain ATCC 23365 / NCT...	[more]
ENO_BRUAB	4.675e-51	54.69	Enolase OS=Brucella abortus GN=eno PE=3 SV=1	[more]
ENO_BRUA2	4.675e-51	54.69	Enolase OS=Brucella abortus (strain 2308) GN=eno P...	[more]
ENO_BRUA1	4.675e-51	54.69	Enolase OS=Brucella abortus (strain S19) GN=eno PE...	[more]
ENO_ALKMQ	6.093e-51	54.40	Enolase OS=Alkaliphilus metalliredigens (strain QY...	[more]
ENO_THEAB	6.093e-51	55.33	Enolase OS=Thermosipho africanus (strain TCF52B) G...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-704-004-B01-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY728463 ID=EY728463; Name=EY728463; organism=Citrus sinensis; type=EST; length=761bp
CCACGCGTCCGGTTTTCAGAAAATGGCAATAACCATCACTGCCGTCAAAG
CCAGGCAGATCTTTGACAGCCGTGGAAATCCAACGGTGGAGGTCGACGTG
ACAACATCAGATGGCCACGTGGCTAGAGCTGCTGTTCCAAGCGGTGCATC
CACCGGAATTTATGAGGCGCTTGAACTAAGAGACGGAGGCTCTGATTACC
TAGGCAAGGGTGTATCGAAAGCTGTTAGCAATGTCAACGCCATCATTGGC
CCTGCACTCGCTGGCAAGGACCCAACTGAGCAGACTGCTATTGACAACTA
TATGGTACAACAACTTGATGGAACTGTAAATGAGTGGGGTTGGTGCAAAC
AGAAGCTTGGAGCAAACGCCATTTTGGCTGTCTCCCTANCTGTCTGCAAG
GCTGGTGCACATGTCAAGAAGATTCCTCTTTACAAGCATATTGCCGAACT
CTCTGGTAACAAGAATTTGGTGTTGCCAGTGCCAGCATTCAATGTCATTA
ATGGTGGATCACATGCTGGAGACAAACTTGCTATGCAGGAGTTTATGATT
CTTCCTGTAGGAGCTTCCTGTTTCAAAGAACCCATGAAGATGGGTGTAGA
GATATATCACCTCTGAAGGTGTGATTAAAAGAGAAATACGGCCGGGATGC
TACAATGTTGGGGATGAAGGGGGGTTTGCGCCCCATTATCGGGAGGACAA
GGGAGGGATTGAGTTGGTCCACACAGCCGGGGTTAAAGCCGGTTCACCGA
GGCAAAGTGGC