EY728457 | Citrus Genome Database

Overview


Name	EY728457
Unique Name	EY728457
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	845

Libraries


Library Name	Type
Sweet orange fruit, development stadium (5 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY728457 vs. ExPASy Swiss-Prot
Match: NACAM_MOUSE (Nascent polypeptide-associated complex subunit alpha, muscle-specific form OS=Mus musculus GN=Naca PE=1 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 1.367e-33
Identity = 69/116 (59.48%), Postives = 91/116 (78.45%), Query Frame = 3

Query:  222 AMLKLGMKPIPGVSRVTVKKSKNILFVISKPDVFKSPTTDTYVIFGEAKIEDLSSQLQTQAAEQFKAPD--LSHVVSKPESSAMAQ--DDEEVDETGVEPKDIELVMTQAGVSRGK 557
            AM KLG++ + GV+RVT++KSKNILFVI+KPDV+KSP +DTY++FGEAKIEDLS Q Q  AAE+FK     +S++    ++  + +  ++EEVDETGVE KDIELVM+QA VSR K
Sbjct: 2051 AMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAK 2166

HSP 2 Score: 30.4166 bits (67), Expect = 1.367e-33
Identity = 15/22 (68.18%), Postives = 18/22 (81.82%), Query Frame = 1

Query:  553 AKAVEALKAANGDLVSAIMELT 618
            AKAV ALK  + D+V+AIMELT
Sbjct: 2165 AKAVRALKNNSNDIVNAIMELT 2186

[Close]

BLAST of EY728457 vs. ExPASy Swiss-Prot
Match: NACA_MOUSE (Nascent polypeptide-associated complex subunit alpha OS=Mus musculus GN=Naca PE=1 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 1.411e-33
Identity = 69/116 (59.48%), Postives = 91/116 (78.45%), Query Frame = 3

Query:  222 AMLKLGMKPIPGVSRVTVKKSKNILFVISKPDVFKSPTTDTYVIFGEAKIEDLSSQLQTQAAEQFKAPD--LSHVVSKPESSAMAQ--DDEEVDETGVEPKDIELVMTQAGVSRGK 557
            AM KLG++ + GV+RVT++KSKNILFVI+KPDV+KSP +DTY++FGEAKIEDLS Q Q  AAE+FK     +S++    ++  + +  ++EEVDETGVE KDIELVM+QA VSR K
Sbjct:   79 AMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAK 194

HSP 2 Score: 30.4166 bits (67), Expect = 1.411e-33
Identity = 15/22 (68.18%), Postives = 18/22 (81.82%), Query Frame = 1

Query:  553 AKAVEALKAANGDLVSAIMELT 618
            AKAV ALK  + D+V+AIMELT
Sbjct:  193 AKAVRALKNNSNDIVNAIMELT 214

[Close]

BLAST of EY728457 vs. ExPASy Swiss-Prot
Match: NACA_HUMAN (Nascent polypeptide-associated complex subunit alpha OS=Homo sapiens GN=NACA PE=1 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 1.411e-33
Identity = 69/116 (59.48%), Postives = 91/116 (78.45%), Query Frame = 3

Query:  222 AMLKLGMKPIPGVSRVTVKKSKNILFVISKPDVFKSPTTDTYVIFGEAKIEDLSSQLQTQAAEQFKAPD--LSHVVSKPESSAMAQ--DDEEVDETGVEPKDIELVMTQAGVSRGK 557
            AM KLG++ + GV+RVT++KSKNILFVI+KPDV+KSP +DTY++FGEAKIEDLS Q Q  AAE+FK     +S++    ++  + +  ++EEVDETGVE KDIELVM+QA VSR K
Sbjct:   79 AMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAK 194

HSP 2 Score: 30.4166 bits (67), Expect = 1.411e-33
Identity = 15/22 (68.18%), Postives = 18/22 (81.82%), Query Frame = 1

Query:  553 AKAVEALKAANGDLVSAIMELT 618
            AKAV ALK  + D+V+AIMELT
Sbjct:  193 AKAVRALKNNSNDIVNAIMELT 214

[Close]

BLAST of EY728457 vs. ExPASy Swiss-Prot
Match: NACA_BOVIN (Nascent polypeptide-associated complex subunit alpha OS=Bos taurus GN=NACA PE=1 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 1.411e-33
Identity = 69/116 (59.48%), Postives = 91/116 (78.45%), Query Frame = 3

Query:  222 AMLKLGMKPIPGVSRVTVKKSKNILFVISKPDVFKSPTTDTYVIFGEAKIEDLSSQLQTQAAEQFKAPD--LSHVVSKPESSAMAQ--DDEEVDETGVEPKDIELVMTQAGVSRGK 557
            AM KLG++ + GV+RVT++KSKNILFVI+KPDV+KSP +DTY++FGEAKIEDLS Q Q  AAE+FK     +S++    ++  + +  ++EEVDETGVE KDIELVM+QA VSR K
Sbjct:   79 AMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAK 194

HSP 2 Score: 30.4166 bits (67), Expect = 1.411e-33
Identity = 15/22 (68.18%), Postives = 18/22 (81.82%), Query Frame = 1

Query:  553 AKAVEALKAANGDLVSAIMELT 618
            AKAV ALK  + D+V+AIMELT
Sbjct:  193 AKAVRALKNNSNDIVNAIMELT 214

[Close]

BLAST of EY728457 vs. ExPASy Swiss-Prot
Match: NACA_XENTR (Nascent polypeptide-associated complex subunit alpha OS=Xenopus tropicalis GN=naca PE=2 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 1.411e-33
Identity = 69/116 (59.48%), Postives = 91/116 (78.45%), Query Frame = 3

Query:  222 AMLKLGMKPIPGVSRVTVKKSKNILFVISKPDVFKSPTTDTYVIFGEAKIEDLSSQLQTQAAEQFKAPD--LSHVVSKPESSAMAQ--DDEEVDETGVEPKDIELVMTQAGVSRGK 557
            AM KLG++ + GV+RVT++KSKNILFVI+KPDV+KSP +DTY++FGEAKIEDLS Q Q  AAE+FK     +S++    ++  + +  ++EEVDETGVE KDIELVM+QA VSR K
Sbjct:   78 AMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAK 193

HSP 2 Score: 30.4166 bits (67), Expect = 1.411e-33
Identity = 15/22 (68.18%), Postives = 18/22 (81.82%), Query Frame = 1

Query:  553 AKAVEALKAANGDLVSAIMELT 618
            AKAV ALK  + D+V+AIMELT
Sbjct:  192 AKAVRALKNNSNDIVNAIMELT 213

[Close]

BLAST of EY728457 vs. ExPASy Swiss-Prot
Match: NACA_XENLA (Nascent polypeptide-associated complex subunit alpha OS=Xenopus laevis GN=naca PE=2 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 5.289e-33
Identity = 68/116 (58.62%), Postives = 90/116 (77.59%), Query Frame = 3

Query:  222 AMLKLGMKPIPGVSRVTVKKSKNILFVISKPDVFKSPTTDTYVIFGEAKIEDLSSQLQTQAAEQFK--APDLSHVVSKPESSAMAQ--DDEEVDETGVEPKDIELVMTQAGVSRGK 557
            AM KLG++ + GV+RVT++KSKNILFVI+KPDV+KSP +DTY++FGEAKIEDLS Q Q  AAE+FK     +S++    ++  + +  ++EEVDE GVE KDIELVM+QA VSR K
Sbjct:   77 AMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGETVSNIQENTQTPTVQEESEEEEVDEAGVEVKDIELVMSQANVSRAK 192

HSP 2 Score: 30.4166 bits (67), Expect = 5.289e-33
Identity = 15/22 (68.18%), Postives = 18/22 (81.82%), Query Frame = 1

Query:  553 AKAVEALKAANGDLVSAIMELT 618
            AKAV ALK  + D+V+AIMELT
Sbjct:  191 AKAVRALKNNSNDIVNAIMELT 212

[Close]

BLAST of EY728457 vs. ExPASy Swiss-Prot
Match: NACA_PONAB (Nascent polypeptide-associated complex subunit alpha OS=Pongo abelii GN=NACA PE=2 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 8.968e-33
Identity = 68/116 (58.62%), Postives = 90/116 (77.59%), Query Frame = 3

Query:  222 AMLKLGMKPIPGVSRVTVKKSKNILFVISKPDVFKSPTTDTYVIFGEAKIEDLSSQLQTQAAEQFKAPD--LSHVVSKPESSAMAQ--DDEEVDETGVEPKDIELVMTQAGVSRGK 557
            AM KLG++ + GV+RVT++KSKNILFVI+KPDV+KSP +DTY++FGEAKIEDLS Q Q  AAE+FK     +S++    ++  + +  ++EEVDET VE KDIELVM+QA VSR K
Sbjct:   79 AMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETDVEVKDIELVMSQANVSRAK 194

HSP 2 Score: 30.4166 bits (67), Expect = 8.968e-33
Identity = 15/22 (68.18%), Postives = 18/22 (81.82%), Query Frame = 1

Query:  553 AKAVEALKAANGDLVSAIMELT 618
            AKAV ALK  + D+V+AIMELT
Sbjct:  193 AKAVRALKNNSNDIVNAIMELT 214

[Close]

BLAST of EY728457 vs. ExPASy Swiss-Prot
Match: NACA_CHILA (Nascent polypeptide-associated complex subunit alpha OS=Chinchilla lanigera PE=2 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 2.580e-32
Identity = 68/116 (58.62%), Postives = 90/116 (77.59%), Query Frame = 3

Query:  222 AMLKLGMKPIPGVSRVTVKKSKNILFVISKPDVFKSPTTDTYVIFGEAKIEDLSSQLQTQAAEQFKAPD--LSHVVSKPESSAMAQ--DDEEVDETGVEPKDIELVMTQAGVSRGK 557
            AM KLG++ + GV+RVT++KSKNILFVI+KPDV+KSP +DTY++FGEAKIEDLS Q Q  AAE+FK     +S++    ++  + +  ++EEVDETGVE KDIE VM+QA VSR K
Sbjct:   79 AMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIEWVMSQANVSRAK 194

HSP 2 Score: 28.4906 bits (62), Expect = 2.580e-32
Identity = 14/22 (63.64%), Postives = 18/22 (81.82%), Query Frame = 1

Query:  553 AKAVEALKAANGDLVSAIMELT 618
            AKAV ALK  + ++V+AIMELT
Sbjct:  193 AKAVRALKNNSNNIVNAIMELT 214

[Close]

BLAST of EY728457 vs. ExPASy Swiss-Prot
Match: NACAD_HUMAN (NAC-alpha domain-containing protein 1 OS=Homo sapiens GN=NACAD PE=2 SV=3)

HSP 1 Score: 129.798 bits (325), Expect = 7.208e-32
Identity = 70/123 (56.91%), Postives = 88/123 (71.54%), Query Frame = 3

Query:  222 AMLKLGMKPIPGVSRVTVKKSKNILFVISKPDVFKSPTTDTYVIFGEAKIEDLSSQLQTQAAEQFKAPDLSHVVSKPESSAM-----------AQDDEEVDETGVEPKDIELVMTQAGVSRGK 557
            AM KLG++ I GV+R+T++KSKNILFVI+KPDVFKSP +DTYV+FGEAKIEDLS Q+   AAE+FK P     +  PES+              +++EEVDE G+E +DIELVM QA VSR K
Sbjct: 1420 AMSKLGLRQIQGVTRITIQKSKNILFVIAKPDVFKSPASDTYVVFGEAKIEDLSQQVHKAAAEKFKVPSEPSAL-VPESAPRPRVRLECKEEEEEEEEEVDEAGLELRDIELVMAQANVSRAK 1541

HSP 2 Score: 29.261 bits (64), Expect = 7.208e-32
Identity = 14/22 (63.64%), Postives = 18/22 (81.82%), Query Frame = 1

Query:  553 AKAVEALKAANGDLVSAIMELT 618
            AKAV AL+  + D+V+AIMELT
Sbjct: 1540 AKAVRALRDNHSDIVNAIMELT 1561

[Close]

BLAST of EY728457 vs. ExPASy Swiss-Prot
Match: NACP1_HUMAN (Putative nascent polypeptide-associated complex subunit alpha-like protein OS=Homo sapiens GN=NACAP1 PE=5 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 4.715e-31
Identity = 64/112 (57.14%), Postives = 87/112 (77.68%), Query Frame = 3

Query:  222 AMLKLGMKPIPGVSRVTVKKSKNILFVISKPDVFKSPTTDTYVIFGEAKIEDLSSQLQTQAAEQFKAPD--LSHVVSKPESSAMAQ--DDEEVDETGVEPKDIELVMTQAGV 545
            A  KLG++ + GV+RVT++KSKNILFVI+KPDV+KSP +DTY++FGEAKIEDLS + Q  AAE+FK     +S++    ++  + +  +DEEVDETGVE KDIELV++QA V
Sbjct:   78 ARFKLGLQQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYMVFGEAKIEDLSQEAQLAAAEKFKVQGEAVSNIQENTQTPTVQEGSEDEEVDETGVEIKDIELVLSQANV 189

HSP 2 Score: 31.187 bits (69), Expect = 4.715e-31
Identity = 17/23 (73.91%), Postives = 20/23 (86.96%), Query Frame = 1

Query:  550 GAKAVEALKAANGDLVSAIMELT 618
            GAKAV ALK +N D+V+AIMELT
Sbjct:  191 GAKAVRALKNSN-DIVNAIMELT 212

The following BLAST results are available for this feature:

BLAST of EY728457 vs. ExPASy Swiss-Prot

BLAST of EY728457 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 56


Match Name	E-value	Identity	Description
NACAM_MOUSE	1.367e-33	59.48	Nascent polypeptide-associated complex subunit alp...	[more]
NACA_MOUSE	1.411e-33	59.48	Nascent polypeptide-associated complex subunit alp...	[more]
NACA_HUMAN	1.411e-33	59.48	Nascent polypeptide-associated complex subunit alp...	[more]
NACA_BOVIN	1.411e-33	59.48	Nascent polypeptide-associated complex subunit alp...	[more]
NACA_XENTR	1.411e-33	59.48	Nascent polypeptide-associated complex subunit alp...	[more]
NACA_XENLA	5.289e-33	58.62	Nascent polypeptide-associated complex subunit alp...	[more]
NACA_PONAB	8.968e-33	58.62	Nascent polypeptide-associated complex subunit alp...	[more]
NACA_CHILA	2.580e-32	58.62	Nascent polypeptide-associated complex subunit alp...	[more]
NACAD_HUMAN	7.208e-32	56.91	NAC-alpha domain-containing protein 1 OS=Homo sapi...	[more]
NACP1_HUMAN	4.715e-31	57.14	Putative nascent polypeptide-associated complex su...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C3-704-004-A04-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY728457 ID=EY728457; Name=EY728457; organism=Citrus sinensis; type=EST; length=845bp
CCACGCGTCCGACTGCTCAGACCCAAGAAGAGCTTCTCGCCTCTCTTCTC
GAACAGCAAAAAATCGATGATGATAAGCCTGTTGTAGAAGATGATGAAGA
TGAAGACGAGGATGAGGATGAAGATGATGAGAAAGACGAACATGAAGCTG
ACGAGGGACACCGAGATGGAGAGGCAGGTGGTAGGTCAAAACAGAGCAGA
AGTGAAAAGAAGAGTCGCAAAGCAATGTTAAAGCTTGGAATGAAACCTAT
CCCTGGTGTTAGCCGGGTTACCGTCAAAAAGAGCAAGAATATCTTGTTTG
TTATCTCAAAACCGGATGTTTTCAAGAGCCCGACAACAGATACATATGTA
ATCTTTGGTGAGGCAAAGATCGAGGACTTGAGCTCACAATTGCAGACTCA
GGCAGCAGAGCAATTTAAGGCTCCTGATCTCAGCCACGTGGTCTCCAAAC
CTGAGTCATCAGCCATGGCTCAGGACGATGAAGAAGTAGATGAGACCGGG
GTTGAGCCTAAGGACATAGAGTTAGTAATGACACAGGCAGGAGTCTCCAG
GGGCAAAGGCTGTGGAAGCGCTGAAGGCTGCAAATGGGGACCTAGTTTCA
GCTATCATGGAGCTTACCAACTGGAGAGTTTCCTTAGATGGTTGGTGGTG
GATTAATAAACCCTTCCTATATTTTCCGGGTCAGGTGTCGTCTAACTCGC
CCCCTCTCTCCTCGCCCCCGCGGTTGGAAGGGTTCTGCCCTCCGCTTGGN
NGGGNTNAGCCAANNNCCCNNGGGNGGGGGGGCTTTTTGGAGGAGGAAAG
GGGAGGGGAGTTTTCCTCCGGGAAGGCGGGTTTTCTCCTACGCGG