EY728433

Overview
NameEY728433
Unique NameEY728433
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length691
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: RPE_RHORU (Ribulose-phosphate 3-epimerase OS=Rhodospirillum rubrum GN=cbbE PE=3 SV=2)

HSP 1 Score: 127.872 bits (320), Expect = 5.090e-29
Identity = 61/96 (63.54%), Postives = 71/96 (73.96%), Query Frame = -3 
Query:    6 MPTTTRITPSILSADFARLGEEVRDVVAAGADWIHFDVMDNHYVPNLTIGPMVCAAIRPHVSVPIDVHLMVEPVDALVPMFAKAGANYISFHPEAS 293
            M    RI PS+LSADFA            GAD +HFDVMDNHYVPNLT+GP+VCAA+RPH S+PIDVHLM  PVD L+  FA+AGA+ I+FHPEAS
Sbjct:    1 MTRAIRIAPSLLSADFAISRPRCPSDGRTGADILHFDVMDNHYVPNLTVGPLVCAALRPHTSLPIDVHLMTRPVDPLIDSFAEAGADMITFHPEAS 96
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: RPE_BACSU (Ribulose-phosphate 3-epimerase OS=Bacillus subtilis GN=rpe PE=3 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 2.365e-26
Identity = 53/90 (58.89%), Postives = 68/90 (75.56%), Query Frame = -3 
Query:    9 RITPSILSADFARLGEEVRDVVAAGADWIHFDVMDNHYVPNLTIGPMVCAAIRPHVSVPIDVHLMVEPVDALVPMFAKAGANYISFHPEA 278
            ++ PSILSADFA LG E++DV   GAD IH DVMD H+VPN+TIGP++  A+RP   +P+DVHLM+E  D  +P FAKAGA+ +S H EA
Sbjct:    3 KVAPSILSADFAALGNEIKDVEKGGADCIHIDVMDGHFVPNITIGPLIVEAVRPVTDLPLDVHLMIEEPDRYIPAFAKAGADILSVHAEA 92
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_BORPE (Protein apaG OS=Bordetella pertussis GN=apaG PE=1 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 3.414e-25
Identity = 56/98 (57.14%), Postives = 66/98 (67.35%), Query Frame = 3 
Query:  345 ATQERTVKPYDLSVSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDGTSACRKCAGWAWSAXSPCWRPAKTSN*PXAVPSPPRSG 638
            + +ER VKPYDL+VSV+PR+VPEQSDP +QQ+VFAYTVRITNTG HPAQVISRHWIITDG    ++  G       P   P +T       P P   G
Sbjct:    2 SNRERPVKPYDLTVSVTPRYVPEQSDPSQQQYVFAYTVRITNTGSHPAQVISRHWIITDGEERVQEVRGLGVVGQQPLLAPGETFEYTSGCPLPTPIG 99

HSP 2 Score: 98.5969 bits (244), Expect = 3.336e-20
Identity = 44/54 (81.48%), Postives = 46/54 (85.19%), Query Frame = 2 
Query:  527 QRVQEVRGLGVVGXQPLLAPGENFELTXGCPLPTPVGTMGGAYHCFXENGIPFE 688
            +RVQEVRGLGVVG QPLLAPGE FE T GCPLPTP+GTM G YHC  ENGIPFE
Sbjct:   63 ERVQEVRGLGVVGQQPLLAPGETFEYTSGCPLPTPIGTMRGTYHCVGENGIPFE 116
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_BORPA (Protein apaG OS=Bordetella parapertussis GN=apaG PE=3 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 3.414e-25
Identity = 56/98 (57.14%), Postives = 66/98 (67.35%), Query Frame = 3 
Query:  345 ATQERTVKPYDLSVSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDGTSACRKCAGWAWSAXSPCWRPAKTSN*PXAVPSPPRSG 638
            + +ER VKPYDL+VSV+PR+VPEQSDP +QQ+VFAYTVRITNTG HPAQVISRHWIITDG    ++  G       P   P +T       P P   G
Sbjct:    2 SNRERPVKPYDLTVSVTPRYVPEQSDPSQQQYVFAYTVRITNTGSHPAQVISRHWIITDGEERVQEVRGLGVVGQQPLLAPGETFEYTSGCPLPTPIG 99

HSP 2 Score: 98.5969 bits (244), Expect = 3.336e-20
Identity = 44/54 (81.48%), Postives = 46/54 (85.19%), Query Frame = 2 
Query:  527 QRVQEVRGLGVVGXQPLLAPGENFELTXGCPLPTPVGTMGGAYHCFXENGIPFE 688
            +RVQEVRGLGVVG QPLLAPGE FE T GCPLPTP+GTM G YHC  ENGIPFE
Sbjct:   63 ERVQEVRGLGVVGQQPLLAPGETFEYTSGCPLPTPIGTMRGTYHCVGENGIPFE 116
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_BORBR (Protein apaG OS=Bordetella bronchiseptica GN=apaG PE=3 SV=2)

HSP 1 Score: 115.161 bits (287), Expect = 3.414e-25
Identity = 56/98 (57.14%), Postives = 66/98 (67.35%), Query Frame = 3 
Query:  345 ATQERTVKPYDLSVSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDGTSACRKCAGWAWSAXSPCWRPAKTSN*PXAVPSPPRSG 638
            + +ER VKPYDL+VSV+PR+VPEQSDP +QQ+VFAYTVRITNTG HPAQVISRHWIITDG    ++  G       P   P +T       P P   G
Sbjct:    2 SNRERPVKPYDLTVSVTPRYVPEQSDPSQQQYVFAYTVRITNTGSHPAQVISRHWIITDGEERVQEVRGLGVVGQQPLLAPGETFEYTSGCPLPTPIG 99

HSP 2 Score: 98.5969 bits (244), Expect = 3.336e-20
Identity = 44/54 (81.48%), Postives = 46/54 (85.19%), Query Frame = 2 
Query:  527 QRVQEVRGLGVVGXQPLLAPGENFELTXGCPLPTPVGTMGGAYHCFXENGIPFE 688
            +RVQEVRGLGVVG QPLLAPGE FE T GCPLPTP+GTM G YHC  ENGIPFE
Sbjct:   63 ERVQEVRGLGVVGQQPLLAPGETFEYTSGCPLPTPIGTMRGTYHCVGENGIPFE 116
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: RPE_RHOCA (Ribulose-phosphate 3-epimerase OS=Rhodobacter capsulatus GN=cbbE PE=3 SV=2)

HSP 1 Score: 112.849 bits (281), Expect = 1.695e-24
Identity = 51/90 (56.67%), Postives = 65/90 (72.22%), Query Frame = -3 
Query:    9 RITPSILSADFARLGEEVRDVVAAGADWIHFDVMDNHYVPNLTIGPMVCAAIRPHVSVPIDVHLMVEPVDALVPMFAKAGANYISFHPEA 278
            +I PSILSADFA  G E++ + A GADW+H DVMD H+VPNLT GP   AA R HV   +DVHLM+ PVDA +  +A+AGA+ ++ H EA
Sbjct:    8 KIAPSILSADFADFGREIQAIEAQGADWVHVDVMDGHFVPNLTFGPPAVAAFRKHVKTVMDVHLMISPVDAYIDAYAQAGADVLTAHVEA 97
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: RPE_ORYSJ (Ribulose-phosphate 3-epimerase, chloroplastic OS=Oryza sativa subsp. japonica GN=RPE PE=1 SV=2)

HSP 1 Score: 108.612 bits (270), Expect = 3.196e-23
Identity = 50/90 (55.56%), Postives = 67/90 (74.44%), Query Frame = -3 
Query:    6 ITPSILSADFARLGEEVRDVVAAGADWIHFDVMDNHYVPNLTIGPMVCAAIRPHVSVPIDVHLMVEPVDALVPMFAKAGANYISFHPEAS 275
            ++PSILSA+F++LGE+V+ V  AG DWIH DVMD  +VPN+TIGP+V  A+RP   +P+DVHLM+   +  VP F KAGA+ +S H E S
Sbjct:   53 VSPSILSANFSKLGEQVKAVEVAGCDWIHVDVMDGRFVPNITIGPLVVDALRPVTDLPLDVHLMIVEPEQRVPDFIKAGADIVSVHCEQS 142
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: RPE_SPIOL (Ribulose-phosphate 3-epimerase, chloroplastic OS=Spinacia oleracea GN=RPE PE=1 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 4.174e-23
Identity = 50/88 (56.82%), Postives = 66/88 (75.00%), Query Frame = -3 
Query:   12 ITPSILSADFARLGEEVRDVVAAGADWIHFDVMDNHYVPNLTIGPMVCAAIRPHVSVPIDVHLMVEPVDALVPMFAKAGANYISFHPE 275
            ++PSILSA+FA+LGE+V+ V  AG DWIH DVMD  +VPN+TIGP+V  A+RP   +P+DVHLM+   +  VP F KAGA+ +S H E
Sbjct:   64 VSPSILSANFAKLGEQVKAVELAGCDWIHVDVMDGRFVPNITIGPLVVDALRPVTDLPLDVHLMIVEPEQRVPDFIKAGADIVSVHCE 151
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: RPE_SOLTU (Ribulose-phosphate 3-epimerase, chloroplastic (Fragment) OS=Solanum tuberosum PE=1 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 4.174e-23
Identity = 49/90 (54.44%), Postives = 67/90 (74.44%), Query Frame = -3 
Query:    6 ITPSILSADFARLGEEVRDVVAAGADWIHFDVMDNHYVPNLTIGPMVCAAIRPHVSVPIDVHLMVEPVDALVPMFAKAGANYISFHPEAS 275
            ++PSILSA+F++LGE+V+ +  AG DWIH DVMD  +VPN+TIGP+V  ++RP   +P+DVHLM+   D  VP F KAGA+ +S H E S
Sbjct:   59 VSPSILSANFSKLGEQVKAIEQAGCDWIHVDVMDGRFVPNITIGPLVVDSLRPITDLPLDVHLMIVEPDQRVPDFIKAGADIVSVHCEQS 148
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: RPE_SYNY3 (Ribulose-phosphate 3-epimerase OS=Synechocystis sp. (strain PCC 6803) GN=rpe PE=1 SV=3)

HSP 1 Score: 107.842 bits (268), Expect = 5.451e-23
Identity = 52/88 (59.09%), Postives = 64/88 (72.73%), Query Frame = -3 
Query:   12 ITPSILSADFARLGEEVRDVVAAGADWIHFDVMDNHYVPNLTIGPMVCAAIRPHVSVPIDVHLMVEPVDALVPMFAKAGANYISFHPE 275
            + PSILSADF+RLGEE++ V  AGADWIH DVMD  +VPN+TIGP++  AIRP     +DVHLM+   +  V  FAKAGA+ IS H E
Sbjct:    7 VAPSILSADFSRLGEEIKAVDEAGADWIHVDVMDGRFVPNITIGPLIVDAIRPLTKKTLDVHLMIVEPEKYVEDFAKAGADIISVHVE 94
The following BLAST results are available for this feature:
BLAST of EY728433 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 151
Match NameE-valueIdentityDescription
RPE_RHORU5.090e-2963.54Ribulose-phosphate 3-epimerase OS=Rhodospirillum r... [more]
RPE_BACSU2.365e-2658.89Ribulose-phosphate 3-epimerase OS=Bacillus subtili... [more]
APAG_BORPE3.414e-2557.14Protein apaG OS=Bordetella pertussis GN=apaG PE=1 ... [more]
APAG_BORPA3.414e-2557.14Protein apaG OS=Bordetella parapertussis GN=apaG P... [more]
APAG_BORBR3.414e-2557.14Protein apaG OS=Bordetella bronchiseptica GN=apaG ... [more]
RPE_RHOCA1.695e-2456.67Ribulose-phosphate 3-epimerase OS=Rhodobacter caps... [more]
RPE_ORYSJ3.196e-2355.56Ribulose-phosphate 3-epimerase, chloroplastic OS=O... [more]
RPE_SPIOL4.174e-2356.82Ribulose-phosphate 3-epimerase, chloroplastic OS=S... [more]
RPE_SOLTU4.174e-2354.44Ribulose-phosphate 3-epimerase, chloroplastic (Fra... [more]
RPE_SYNY35.451e-2359.09Ribulose-phosphate 3-epimerase OS=Synechocystis sp... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-003-F06-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY728433 ID=EY728433; Name=EY728433; organism=Citrus sinensis; type=EST; length=691bp
TGGCGGCTGGCTTCCGGGTGGAAGCTGATGTAGTTGGCGCCGGCCTTGGC
GAACATCGGCACCAGGGCGTCCACCGGCTCGACCATGAGGTGCACGTCGA
TCGGCACGGAAACATGGGGACGGATCGCCGCGCAGACCATCGGGCCGATG
GTCAGGTTGGGCACGTAGTGGTTGTCCATCACATCGAAATGGATCCAGTC
GGCGCCGGCGGCGACGACGTCACGCACTTCCTCGCCCAGGCGGGCGAAGT
CGGCGGACAGGATGCTGGGGGTGATGCGGGTGGTGGTAGGCATGGCGTTC
GGGGTGGGTGAAGGCATAATGTGCCATTATTGCAGCTTGCTCGTGCCACA
CAGGAAAGGACTGTGAAACCCTACGACCTGAGCGTCTCCGTCTCGCCGCG
CTTCGTGCCAGAACAGTCCGATCCGGGTGAGCAGCAGTTCGTGTTCGCCT
ATACCGTCCGCATCACCAATACCGGCGAGCATCCGGCCCAGGTGATCAGC
CGCCACTGGATTATCACCGATGGCACCAGCGCGTGCAGGAAGTGCGCGGG
CTGGGCGTGGTCGGCCANCAGCCCCTGCTGGCGCCCGGCGAAAACTTCGA
ACTAACCANCGGCTGTCCCCTCCCCACCCCGGTCGGGACGATGGGCGGCG
CCTACCATTGCTTCNGCGAAAACGGGATCCCGTTCGAANTG
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