EY728433

Overview
NameEY728433
Unique NameEY728433
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length691
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_SHESW (Protein apaG OS=Shewanella sp. (strain W3-18-1) GN=apaG PE=3 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 1.413e-13
Identity = 21/58 (36.21%), Postives = 36/58 (62.07%), Query Frame = 3 
Query:  378 LSVSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDGTSACRKCAG 551
            + V V   ++ +QS P +++++F+YT+ I N GE  A++ +RHWIITD      +  G
Sbjct:    8 IRVEVKTEYIEQQSSPEDEKYLFSYTITIINLGEQAAKLETRHWIITDANGNTSEVQG 65

HSP 2 Score: 44.669 bits (104), Expect = 1.413e-13
Identity = 22/50 (44.00%), Postives = 28/50 (56.00%), Query Frame = 2 
Query:  539 EVRGLGVVGXQPLLAPGENFELTXGCPLPTPVGTMGGAYHCFXENGIPFE 688
            EV+G GVVG  P + P   ++ T G  L TP+G M G Y    E+G  FE
Sbjct:   62 EVQGAGVVGETPTIPPNTAYQYTSGTLLDTPLGIMHGTYGMVSESGERFE 111
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_SHEPC (Protein apaG OS=Shewanella putrefaciens (strain CN-32 / ATCC BAA-453) GN=apaG PE=3 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 1.413e-13
Identity = 21/58 (36.21%), Postives = 36/58 (62.07%), Query Frame = 3 
Query:  378 LSVSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDGTSACRKCAG 551
            + V V   ++ +QS P +++++F+YT+ I N GE  A++ +RHWIITD      +  G
Sbjct:    8 IRVEVKTEYIEQQSSPEDEKYLFSYTITIINLGEQAAKLETRHWIITDANGNTSEVQG 65

HSP 2 Score: 44.669 bits (104), Expect = 1.413e-13
Identity = 22/50 (44.00%), Postives = 28/50 (56.00%), Query Frame = 2 
Query:  539 EVRGLGVVGXQPLLAPGENFELTXGCPLPTPVGTMGGAYHCFXENGIPFE 688
            EV+G GVVG  P + P   ++ T G  L TP+G M G Y    E+G  FE
Sbjct:   62 EVQGAGVVGETPTIPPNTAYQYTSGTLLDTPLGIMHGTYGMVSESGERFE 111
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: ALSE_ECOLI (D-allulose-6-phosphate 3-epimerase OS=Escherichia coli (strain K12) GN=alsE PE=1 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.758e-13
Identity = 32/89 (35.96%), Postives = 56/89 (62.92%), Query Frame = -3 
Query:   12 RITPSILSADFARLGEEVRDVVAAGADWIHFDVMDNHYVPNLTIGPMVCAAIRPHVSVPIDVHLMVEPVDALVPMFAKAGANYISFHPE 278
            +I+PS++  D  +  E++ + + + AD+ H D+MD H+VPNLT+ P   + ++   + P+D HLMV      +   A+AGA++I+ HPE
Sbjct:    2 KISPSLMCMDLLKFKEQI-EFIDSHADYFHIDIMDGHFVPNLTLSPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPE 89
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_SHEON (Protein apaG OS=Shewanella oneidensis GN=apaG PE=1 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 4.027e-13
Identity = 21/58 (36.21%), Postives = 36/58 (62.07%), Query Frame = 3 
Query:  378 LSVSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDGTSACRKCAG 551
            + V V   ++ +QS P +++++F+YT+ I N GE  A++ +RHWIITD      +  G
Sbjct:    8 IRVEVKTEYIEQQSSPEDEKYLFSYTITIINLGEQAAKLETRHWIITDANGKTSEVQG 65

HSP 2 Score: 42.743 bits (99), Expect = 4.027e-13
Identity = 21/52 (40.38%), Postives = 27/52 (51.92%), Query Frame = 2 
Query:  530 RVQEVRGLGVVGXQPLLAPGENFELTXGCPLPTPVGTMGGAYHCFXENGIPF 685
            +  EV+G GVVG  P + P   ++ T G  L TP G M G Y    E+G  F
Sbjct:   59 KTSEVQGAGVVGETPTIPPNTAYQYTSGTVLDTPFGIMYGTYGMVSESGEHF 110
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_ERWT9 (Protein apaG OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=apaG PE=3 SV=1)

HSP 1 Score: 48.9062 bits (115), Expect = 4.027e-13
Identity = 21/47 (44.68%), Postives = 30/47 (63.83%), Query Frame = 3 
Query:  384 VSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDG 524
            V V   ++  QS P E+++VFAY V I N G    Q+I R+W+IT+G
Sbjct:    9 VQVQSAYIESQSAPEEERYVFAYAVTIRNVGRIAVQLIGRYWLITNG 55

HSP 2 Score: 46.9802 bits (110), Expect = 4.027e-13
Identity = 22/42 (52.38%), Postives = 27/42 (64.29%), Query Frame = 2 
Query:  530 RVQEVRGLGVVGXQPLLAPGENFELTXGCPLPTPVGTMGGAY 655
            R  EV+G GVVG QP + PG  ++ T G  L TP+GTM G Y
Sbjct:   58 RETEVQGEGVVGEQPHIEPGGEYQYTSGAVLETPIGTMQGHY 99
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_METPB (Protein apaG OS=Methylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001) GN=apaG PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 6.679e-13
Identity = 38/98 (38.78%), Postives = 51/98 (52.04%), Query Frame = 3 
Query:  378 LSVSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDGTSACRKCAGWAWSAXSPCWRPAK----TSN*PXAVPSPPRSGRWAAPTI 659
            ++V+V PRFV E+S PGE ++ FAYTV I N G    Q+ SRHW I DG  AC++  G       P   P +    TS  P   P    +G +   T+
Sbjct:    9 ITVTVEPRFVEEESSPGESRYFFAYTVEIVNNGSEQVQLRSRHWRIIDGRGACQEVRGAGVVGKQPVLEPGESFSYTSGCPLTTPDGLMAGSYTMSTV 106
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_PROMH (Protein apaG OS=Proteus mirabilis (strain HI4320) GN=apaG PE=3 SV=1)

HSP 1 Score: 48.1358 bits (113), Expect = 6.797e-13
Identity = 18/48 (37.50%), Postives = 33/48 (68.75%), Query Frame = 3 
Query:  378 LSVSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITD 521
            +++ V   ++  QS P EQ++VFAYT+ I N  +H  +++ R+W+IT+
Sbjct:    7 VAIQVQSVYIESQSSPEEQRYVFAYTITIHNLNKHAIRLLRRYWLITN 54

HSP 2 Score: 46.9802 bits (110), Expect = 6.797e-13
Identity = 23/50 (46.00%), Postives = 28/50 (56.00%), Query Frame = 2 
Query:  539 EVRGLGVVGXQPLLAPGENFELTXGCPLPTPVGTMGGAYHCFXENGIPFE 688
            EV+G GVVG QPL+  G  +  T G  L TP+GTM G Y      G  F+
Sbjct:   61 EVQGEGVVGEQPLIEAGSRYRYTSGAVLETPMGTMEGHYEMIDAQGRLFQ 110
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_METEP (Protein apaG OS=Methylobacterium extorquens (strain PA1) GN=apaG PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 8.723e-13
Identity = 39/98 (39.80%), Postives = 50/98 (51.02%), Query Frame = 3 
Query:  378 LSVSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDGTSACRKCAGWAWSAXSPCWRPAK----TSN*PXAVPSPPRSGRWAAPTI 659
            + V+V PRFV E+S PGE ++ FAYTV I N G    Q+ SRHW I DG  AC++  G       P   P +    TS  P   P    +G +   TI
Sbjct:    9 IMVTVEPRFVEEESSPGESRYFFAYTVEIVNNGSEQVQLRSRHWRIIDGRGACQEVRGAGVVGKQPVLEPGESFSYTSGCPLTTPDGLMAGSYTMSTI 106
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_METC4 (Protein apaG OS=Methylobacterium chloromethanicum (strain CM4 / NCIMB 13688) GN=apaG PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 8.723e-13
Identity = 39/98 (39.80%), Postives = 50/98 (51.02%), Query Frame = 3 
Query:  378 LSVSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDGTSACRKCAGWAWSAXSPCWRPAK----TSN*PXAVPSPPRSGRWAAPTI 659
            + V+V PRFV E+S PGE ++ FAYTV I N G    Q+ SRHW I DG  AC++  G       P   P +    TS  P   P    +G +   TI
Sbjct:    9 IMVTVEPRFVEEESSPGESRYFFAYTVEIVNNGSEQVQLRSRHWRIIDGRGACQEVRGAGVVGKQPVLEPGESFSYTSGCPLTTPDGLMAGSYTMSTI 106
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_AZOSE (Protein apaG OS=Azoarcus sp. (strain EbN1) GN=apaG PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 8.723e-13
Identity = 34/74 (45.95%), Postives = 44/74 (59.46%), Query Frame = 3 
Query:  372 YDLSVSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDGTSACRKCAGWAWSAXSPCWRPAK 593
            Y + V     FVP QSDP E ++VFAY + +TNTGE PAQ+ISRHW+ITDG    ++  G       P   P +
Sbjct:    7 YRIEVEAIAEFVPGQSDPDENRYVFAYHITLTNTGEVPAQLISRHWVITDGAGKVQEVRGLGVIGEQPMLAPGQ 80
The following BLAST results are available for this feature:
BLAST of EY728433 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 151
Match NameE-valueIdentityDescription
APAG_SHESW1.413e-1336.21Protein apaG OS=Shewanella sp. (strain W3-18-1) GN... [more]
APAG_SHEPC1.413e-1336.21Protein apaG OS=Shewanella putrefaciens (strain CN... [more]
ALSE_ECOLI1.758e-1335.96D-allulose-6-phosphate 3-epimerase OS=Escherichia ... [more]
APAG_SHEON4.027e-1336.21Protein apaG OS=Shewanella oneidensis GN=apaG PE=1... [more]
APAG_ERWT94.027e-1344.68Protein apaG OS=Erwinia tasmaniensis (strain DSM 1... [more]
APAG_METPB6.679e-1338.78Protein apaG OS=Methylobacterium populi (strain AT... [more]
APAG_PROMH6.797e-1337.50Protein apaG OS=Proteus mirabilis (strain HI4320) ... [more]
APAG_METEP8.723e-1339.80Protein apaG OS=Methylobacterium extorquens (strai... [more]
APAG_METC48.723e-1339.80Protein apaG OS=Methylobacterium chloromethanicum ... [more]
APAG_AZOSE8.723e-1345.95Protein apaG OS=Azoarcus sp. (strain EbN1) GN=apaG... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-003-F06-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY728433 ID=EY728433; Name=EY728433; organism=Citrus sinensis; type=EST; length=691bp
TGGCGGCTGGCTTCCGGGTGGAAGCTGATGTAGTTGGCGCCGGCCTTGGC
GAACATCGGCACCAGGGCGTCCACCGGCTCGACCATGAGGTGCACGTCGA
TCGGCACGGAAACATGGGGACGGATCGCCGCGCAGACCATCGGGCCGATG
GTCAGGTTGGGCACGTAGTGGTTGTCCATCACATCGAAATGGATCCAGTC
GGCGCCGGCGGCGACGACGTCACGCACTTCCTCGCCCAGGCGGGCGAAGT
CGGCGGACAGGATGCTGGGGGTGATGCGGGTGGTGGTAGGCATGGCGTTC
GGGGTGGGTGAAGGCATAATGTGCCATTATTGCAGCTTGCTCGTGCCACA
CAGGAAAGGACTGTGAAACCCTACGACCTGAGCGTCTCCGTCTCGCCGCG
CTTCGTGCCAGAACAGTCCGATCCGGGTGAGCAGCAGTTCGTGTTCGCCT
ATACCGTCCGCATCACCAATACCGGCGAGCATCCGGCCCAGGTGATCAGC
CGCCACTGGATTATCACCGATGGCACCAGCGCGTGCAGGAAGTGCGCGGG
CTGGGCGTGGTCGGCCANCAGCCCCTGCTGGCGCCCGGCGAAAACTTCGA
ACTAACCANCGGCTGTCCCCTCCCCACCCCGGTCGGGACGATGGGCGGCG
CCTACCATTGCTTCNGCGAAAACGGGATCCCGTTCGAANTG
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