EY728433

Overview
NameEY728433
Unique NameEY728433
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length691
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: RPE_MYCLE (Ribulose-phosphate 3-epimerase OS=Mycobacterium leprae GN=rpe PE=3 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.064e-18
Identity = 46/90 (51.11%), Postives = 61/90 (67.78%), Query Frame = -3 
Query:    6 ITPSILSADFARLGEEVRDVVAAGADWIHFDVMDNHYVPNLTIGPMVCAAIRPHVSVPIDVHLMVEPVDALVPMFAKAGANYISFHPEAS 275
            I PSIL+ADFARL +E    V A ADW+H DVMD H+VPNLTIG  V  ++     +P+D HLM++  D   P +A+AGA  ++FH EA+
Sbjct:    5 IAPSILAADFARLTDEA--AVVASADWLHVDVMDGHFVPNLTIGLPVVQSLLAASDIPMDCHLMIDNPDRWAPPYAEAGAYNVTFHAEAT 92
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: RPE_MYCBO (Ribulose-phosphate 3-epimerase OS=Mycobacterium bovis GN=rpe PE=3 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.064e-18
Identity = 46/90 (51.11%), Postives = 61/90 (67.78%), Query Frame = -3 
Query:    6 ITPSILSADFARLGEEVRDVVAAGADWIHFDVMDNHYVPNLTIGPMVCAAIRPHVSVPIDVHLMVEPVDALVPMFAKAGANYISFHPEAS 275
            I PSIL+ADFARL +E   V   GADW+H DVMD H+VPNLTIG  V  ++     +P+D HLM++  D   P +A+AGA  ++FH EA+
Sbjct:   10 IAPSILAADFARLADEAAAV--NGADWLHVDVMDGHFVPNLTIGLPVVESLLAVTDIPMDCHLMIDNPDRWAPPYAEAGAYNVTFHAEAT 97
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_PSEPK (Protein apaG OS=Pseudomonas putida (strain KT2440) GN=apaG PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.365e-18
Identity = 26/60 (43.33%), Postives = 38/60 (63.33%), Query Frame = 3 
Query:  372 YDLSVSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDGTSACRKCAG 551
            Y + VSV  R++ EQSDP   +F FAYT+ + N G   A+++SRHW+IT+G     +  G
Sbjct:    6 YQIDVSVVTRYLKEQSDPENSRFAFAYTITVQNNGSLSAKLLSRHWLITNGDGEVEEVRG 65

HSP 2 Score: 52.7582 bits (125), Expect = 1.365e-18
Identity = 25/52 (48.08%), Postives = 34/52 (65.38%), Query Frame = 2 
Query:  533 VQEVRGLGVVGXQPLLAPGENFELTXGCPLPTPVGTMGGAYHCFXENGIPFE 688
            V+EVRG GVVG QP + PG++   + G  + T VGTM G+Y  F E+G  F+
Sbjct:   60 VEEVRGAGVVGQQPNIDPGQSHTYSSGAVISTRVGTMQGSYQMFAEDGKRFD 111
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_PSEPG (Protein apaG OS=Pseudomonas putida (strain GB-1) GN=apaG PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.365e-18
Identity = 26/60 (43.33%), Postives = 38/60 (63.33%), Query Frame = 3 
Query:  372 YDLSVSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDGTSACRKCAG 551
            Y + VSV  R++ EQSDP   +F FAYT+ + N G   A+++SRHW+IT+G     +  G
Sbjct:    6 YQIDVSVVTRYLKEQSDPENSRFAFAYTITVQNNGSLKAKLLSRHWLITNGDGEVEEVRG 65

HSP 2 Score: 52.7582 bits (125), Expect = 1.365e-18
Identity = 25/52 (48.08%), Postives = 34/52 (65.38%), Query Frame = 2 
Query:  533 VQEVRGLGVVGXQPLLAPGENFELTXGCPLPTPVGTMGGAYHCFXENGIPFE 688
            V+EVRG GVVG QP + PG++   + G  + T VGTM G+Y  F E+G  F+
Sbjct:   60 VEEVRGAGVVGQQPNIDPGQSHTYSSGAVISTRVGTMQGSYQMFAEDGKRFD 111
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_PSEP1 (Protein apaG OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=apaG PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.365e-18
Identity = 26/60 (43.33%), Postives = 38/60 (63.33%), Query Frame = 3 
Query:  372 YDLSVSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDGTSACRKCAG 551
            Y + VSV  R++ EQSDP   +F FAYT+ + N G   A+++SRHW+IT+G     +  G
Sbjct:    6 YQIDVSVVTRYLKEQSDPENSRFAFAYTITVQNNGSLSAKLLSRHWLITNGDGEVEEVRG 65

HSP 2 Score: 52.7582 bits (125), Expect = 1.365e-18
Identity = 25/52 (48.08%), Postives = 34/52 (65.38%), Query Frame = 2 
Query:  533 VQEVRGLGVVGXQPLLAPGENFELTXGCPLPTPVGTMGGAYHCFXENGIPFE 688
            V+EVRG GVVG QP + PG++   + G  + T VGTM G+Y  F E+G  F+
Sbjct:   60 VEEVRGAGVVGQQPNIDPGQSHTYSSGAVISTRVGTMQGSYQMFAEDGKRFD 111
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_PSEPF (Protein apaG OS=Pseudomonas fluorescens (strain Pf0-1) GN=apaG PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 3.003e-18
Identity = 26/60 (43.33%), Postives = 39/60 (65.00%), Query Frame = 3 
Query:  372 YDLSVSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDGTSACRKCAG 551
            Y + VSV   ++ +QS P  ++F FAYT+ + N GE PA+++SRHW+ITDG     +  G
Sbjct:    6 YQVDVSVVTHYLADQSQPEHERFAFAYTITVQNNGEQPARLMSRHWVITDGDGHVEEVRG 65

HSP 2 Score: 48.9062 bits (115), Expect = 3.003e-18
Identity = 23/52 (44.23%), Postives = 33/52 (63.46%), Query Frame = 2 
Query:  533 VQEVRGLGVVGXQPLLAPGENFELTXGCPLPTPVGTMGGAYHCFXENGIPFE 688
            V+EVRG GVVG QPL+  G++   + G  + T VGTM G+Y    ++G  F+
Sbjct:   60 VEEVRGAGVVGQQPLIGAGKSHTYSSGTVMTTKVGTMQGSYEMVADDGKHFD 111
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_ANADE (Protein apaG OS=Anaeromyxobacter dehalogenans (strain 2CP-C) GN=apaG PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 8.592e-18
Identity = 27/58 (46.55%), Postives = 39/58 (67.24%), Query Frame = 3 
Query:  378 LSVSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDGTSACRKCAG 551
            + V+V   F PE+S+PG  +F+F+Y+VR+ N GE PAQ++SRHWII D      +  G
Sbjct:    9 IEVTVRSTFRPERSEPG--RFLFSYSVRVVNQGEAPAQLVSRHWIIVDANGEREEVVG 64

HSP 2 Score: 51.9878 bits (123), Expect = 8.592e-18
Identity = 26/51 (50.98%), Postives = 32/51 (62.75%), Query Frame = 2 
Query:  536 QEVRGLGVVGXQPLLAPGENFELTXGCPLPTPVGTMGGAYHCFXENGIPFE 688
            +EV G GVVG QP L PGE+FE T  C L TP G+M G Y    ++G  F+
Sbjct:   60 EEVVGDGVVGQQPHLEPGEHFEYTSFCVLKTPHGSMRGTYRMVRDDGQAFD 110
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_SALTI (Protein apaG OS=Salmonella typhi GN=apaG PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.117e-17
Identity = 28/54 (51.85%), Postives = 34/54 (62.96%), Query Frame = 2 
Query:  524 HQRVQEVRGLGVVGXQPLLAPGENFELTXGCPLPTPVGTMGGAYHCFXENGIPF 685
            H R  EV+G GVVG QP +APGE ++ T G  + TP+GTM G Y    ENG  F
Sbjct:   56 HGRETEVQGKGVVGVQPRIAPGEEYQYTGGAVIETPLGTMQGHYEMIDENGDAF 109

HSP 2 Score: 51.6026 bits (122), Expect = 1.117e-17
Identity = 20/47 (42.55%), Postives = 32/47 (68.09%), Query Frame = 3 
Query:  384 VSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDG 524
            + V   ++  QS P ++++VFAYTV I N G  P Q++ R+W+IT+G
Sbjct:    9 IQVQSVYIEAQSSPDDERYVFAYTVTIRNLGRAPVQLLGRYWLITNG 55
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_SALTY (Protein apaG OS=Salmonella typhimurium GN=apaG PE=3 SV=2)

HSP 1 Score: 59.3066 bits (142), Expect = 1.453e-17
Identity = 28/54 (51.85%), Postives = 34/54 (62.96%), Query Frame = 2 
Query:  524 HQRVQEVRGLGVVGXQPLLAPGENFELTXGCPLPTPVGTMGGAYHCFXENGIPF 685
            H R  EV+G GVVG QP +APGE ++ T G  + TP+GTM G Y    ENG  F
Sbjct:   56 HGRETEVQGEGVVGVQPRIAPGEEYQYTSGAVIETPLGTMQGHYEMIDENGDAF 109

HSP 2 Score: 51.6026 bits (122), Expect = 1.453e-17
Identity = 20/47 (42.55%), Postives = 32/47 (68.09%), Query Frame = 3 
Query:  384 VSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDG 524
            + V   ++  QS P ++++VFAYTV I N G  P Q++ R+W+IT+G
Sbjct:    9 IQVQSVYIEAQSSPDDERYVFAYTVTIRNLGRAPVQLLGRYWLITNG 55
[Close]
BLAST of EY728433 vs. ExPASy Swiss-Prot
Match: APAG_SALSV (Protein apaG OS=Salmonella schwarzengrund (strain CVM19633) GN=apaG PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.453e-17
Identity = 28/54 (51.85%), Postives = 34/54 (62.96%), Query Frame = 2 
Query:  524 HQRVQEVRGLGVVGXQPLLAPGENFELTXGCPLPTPVGTMGGAYHCFXENGIPF 685
            H R  EV+G GVVG QP +APGE ++ T G  + TP+GTM G Y    ENG  F
Sbjct:   56 HGRETEVQGEGVVGVQPRIAPGEEYQYTSGAVIETPLGTMQGHYEMIDENGDAF 109

HSP 2 Score: 51.6026 bits (122), Expect = 1.453e-17
Identity = 20/47 (42.55%), Postives = 32/47 (68.09%), Query Frame = 3 
Query:  384 VSVSPRFVPEQSDPGEQQFVFAYTVRITNTGEHPAQVISRHWIITDG 524
            + V   ++  QS P ++++VFAYTV I N G  P Q++ R+W+IT+G
Sbjct:    9 IQVQSVYIEAQSSPDDERYVFAYTVTIRNLGRAPVQLLGRYWLITNG 55
The following BLAST results are available for this feature:
BLAST of EY728433 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 151
Match NameE-valueIdentityDescription
RPE_MYCLE1.064e-1851.11Ribulose-phosphate 3-epimerase OS=Mycobacterium le... [more]
RPE_MYCBO1.064e-1851.11Ribulose-phosphate 3-epimerase OS=Mycobacterium bo... [more]
APAG_PSEPK1.365e-1843.33Protein apaG OS=Pseudomonas putida (strain KT2440)... [more]
APAG_PSEPG1.365e-1843.33Protein apaG OS=Pseudomonas putida (strain GB-1) G... [more]
APAG_PSEP11.365e-1843.33Protein apaG OS=Pseudomonas putida (strain F1 / AT... [more]
APAG_PSEPF3.003e-1843.33Protein apaG OS=Pseudomonas fluorescens (strain Pf... [more]
APAG_ANADE8.592e-1846.55Protein apaG OS=Anaeromyxobacter dehalogenans (str... [more]
APAG_SALTI1.117e-1751.85Protein apaG OS=Salmonella typhi GN=apaG PE=3 SV=1[more]
APAG_SALTY1.453e-1751.85Protein apaG OS=Salmonella typhimurium GN=apaG PE=... [more]
APAG_SALSV1.453e-1751.85Protein apaG OS=Salmonella schwarzengrund (strain ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-003-F06-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY728433 ID=EY728433; Name=EY728433; organism=Citrus sinensis; type=EST; length=691bp
TGGCGGCTGGCTTCCGGGTGGAAGCTGATGTAGTTGGCGCCGGCCTTGGC
GAACATCGGCACCAGGGCGTCCACCGGCTCGACCATGAGGTGCACGTCGA
TCGGCACGGAAACATGGGGACGGATCGCCGCGCAGACCATCGGGCCGATG
GTCAGGTTGGGCACGTAGTGGTTGTCCATCACATCGAAATGGATCCAGTC
GGCGCCGGCGGCGACGACGTCACGCACTTCCTCGCCCAGGCGGGCGAAGT
CGGCGGACAGGATGCTGGGGGTGATGCGGGTGGTGGTAGGCATGGCGTTC
GGGGTGGGTGAAGGCATAATGTGCCATTATTGCAGCTTGCTCGTGCCACA
CAGGAAAGGACTGTGAAACCCTACGACCTGAGCGTCTCCGTCTCGCCGCG
CTTCGTGCCAGAACAGTCCGATCCGGGTGAGCAGCAGTTCGTGTTCGCCT
ATACCGTCCGCATCACCAATACCGGCGAGCATCCGGCCCAGGTGATCAGC
CGCCACTGGATTATCACCGATGGCACCAGCGCGTGCAGGAAGTGCGCGGG
CTGGGCGTGGTCGGCCANCAGCCCCTGCTGGCGCCCGGCGAAAACTTCGA
ACTAACCANCGGCTGTCCCCTCCCCACCCCGGTCGGGACGATGGGCGGCG
CCTACCATTGCTTCNGCGAAAACGGGATCCCGTTCGAANTG
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