EY728351

Overview
NameEY728351
Unique NameEY728351
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length811
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_STRT1 (ATP-dependent Clp protease ATP-binding subunit clpX OS=Streptococcus thermophilus (strain CNRZ 1066) GN=clpX PE=3 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 7.142e-15
Identity = 24/40 (60.00%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF I DAT+LT+AGYVGED E++L +L+ AADF++E  +R
Sbjct:  137 PFAIADATSLTEAGYVGEDVENILLKLIQAADFNIERAER 176

HSP 2 Score: 46.2098 bits (108), Expect = 7.142e-15
Identity = 26/56 (46.43%), Postives = 35/56 (62.50%), Query Frame = 3 
Query:  174 GENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            GEN    R VSGEG+   LLK+++ T  +VPP   G RK P  E I I+TK++ F+
Sbjct:  191 GENVSITRDVSGEGVQQALLKIIEGTVASVPP--QGGRKHPNQEMIQIDTKNILFI 244
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_THET8 (ATP-dependent Clp protease ATP-binding subunit clpX OS=Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) GN=clpX PE=3 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 7.146e-15
Identity = 24/36 (66.67%), Postives = 28/36 (77.78%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVE 120
            PF I DAT LT+AGYVGED E++L +LL  ADF VE
Sbjct:  128 PFAIADATTLTEAGYVGEDVENVLLRLLQNADFDVE 163

HSP 2 Score: 50.8322 bits (120), Expect = 7.146e-15
Identity = 27/55 (49.09%), Postives = 35/55 (63.64%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            ENP   R VSGEG+   LLK+++ T  NVPP   G RK P  E IP+ TK++ F+
Sbjct:  183 ENPSLTRDVSGEGVQQALLKIIEGTVANVPP--QGGRKHPHQEFIPVNTKNILFI 235
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_THET2 (ATP-dependent Clp protease ATP-binding subunit clpX OS=Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) GN=clpX PE=3 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 7.146e-15
Identity = 24/36 (66.67%), Postives = 28/36 (77.78%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVE 120
            PF I DAT LT+AGYVGED E++L +LL  ADF VE
Sbjct:  128 PFAIADATTLTEAGYVGEDVENVLLRLLQNADFDVE 163

HSP 2 Score: 50.8322 bits (120), Expect = 7.146e-15
Identity = 27/55 (49.09%), Postives = 35/55 (63.64%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            ENP   R VSGEG+   LLK+++ T  NVPP   G RK P  E IP+ TK++ F+
Sbjct:  183 ENPSLTRDVSGEGVQQALLKIIEGTVANVPP--QGGRKHPHQEFIPVNTKNILFI 235
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_HALOH (ATP-dependent Clp protease ATP-binding subunit clpX OS=Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) GN=clpX PE=3 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 9.275e-15
Identity = 24/40 (60.00%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF ITDAT+LT+AGYVGED E++L +L+ AAD+ +E  +R
Sbjct:  136 PFAITDATSLTEAGYVGEDVENILLKLIQAADYDIEKAER 175

HSP 2 Score: 45.8246 bits (107), Expect = 9.275e-15
Identity = 26/56 (46.43%), Postives = 35/56 (62.50%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFLS 344
            ENP   R VSGEG+   LLK+L+ T  +VPP   G RK P  E I I+T ++ F++
Sbjct:  191 ENPSITRDVSGEGVQQALLKILEGTVASVPP--QGGRKHPHQEFIQIDTTNILFIA 244
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_STRPS (ATP-dependent Clp protease ATP-binding subunit clpX OS=Streptococcus pneumoniae (strain CGSP14) GN=clpX PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 9.278e-15
Identity = 26/40 (65.00%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF I DATALT+AGYVGED E++L +LL AADF++E  +R
Sbjct:  139 PFAIADATALTEAGYVGEDVENILLKLLQAADFNIERAER 178

HSP 2 Score: 43.8986 bits (102), Expect = 9.278e-15
Identity = 24/55 (43.64%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            EN    R VSGEG+   LLK+++ T  +VPP   G RK P  E I ++TK++ F+
Sbjct:  194 ENVSITRDVSGEGVQQALLKIIEGTVASVPP--QGGRKHPQQEMIQVDTKNILFI 246
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_STRPJ (ATP-dependent Clp protease ATP-binding subunit clpX OS=Streptococcus pneumoniae (strain ATCC 700669 / Spain 23F-1) GN=clpX PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 9.278e-15
Identity = 26/40 (65.00%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF I DATALT+AGYVGED E++L +LL AADF++E  +R
Sbjct:  139 PFAIADATALTEAGYVGEDVENILLKLLQAADFNIERAER 178

HSP 2 Score: 43.8986 bits (102), Expect = 9.278e-15
Identity = 24/55 (43.64%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            EN    R VSGEG+   LLK+++ T  +VPP   G RK P  E I ++TK++ F+
Sbjct:  194 ENVSITRDVSGEGVQQALLKIIEGTVASVPP--QGGRKHPQQEMIQVDTKNILFI 246
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_STRGC (ATP-dependent Clp protease ATP-binding subunit clpX OS=Streptococcus gordonii (strain Challis / ATCC 35105 / CH1 / DL1 / V288) GN=clpX PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 9.278e-15
Identity = 26/40 (65.00%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF I DATALT+AGYVGED E++L +LL AADF++E  +R
Sbjct:  138 PFAIADATALTEAGYVGEDVENILLKLLQAADFNIERAER 177

HSP 2 Score: 43.8986 bits (102), Expect = 9.278e-15
Identity = 24/55 (43.64%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            EN    R VSGEG+   LLK+++ T  +VPP   G RK P  E I ++TK++ F+
Sbjct:  193 ENVSITRDVSGEGVQQALLKIIEGTVASVPP--QGGRKHPQQEMIQVDTKNILFI 245
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_STRU0 (ATP-dependent Clp protease ATP-binding subunit clpX OS=Streptococcus uberis (strain ATCC BAA-854 / 0140J) GN=clpX PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.206e-14
Identity = 24/40 (60.00%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF I DAT+LT+AGYVGED E++L +L+ AAD++VE  +R
Sbjct:  138 PFAIADATSLTEAGYVGEDVENILLKLIQAADYNVERAER 177

HSP 2 Score: 46.2098 bits (108), Expect = 1.206e-14
Identity = 26/56 (46.43%), Postives = 35/56 (62.50%), Query Frame = 3 
Query:  174 GENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            GEN    R VSGEG+   LLK+++ T  +VPP   G RK P  E I I+TK++ F+
Sbjct:  192 GENVSITRDVSGEGVQQALLKIIEGTVASVPP--QGGRKHPNQEMIQIDTKNILFI 245
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_STRS7 (ATP-dependent Clp protease ATP-binding subunit clpX OS=Streptococcus equi subsp. zooepidemicus (strain H70) GN=clpX PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.206e-14
Identity = 24/40 (60.00%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF I DAT+LT+AGYVGED E++L +L+ AAD++VE  +R
Sbjct:  138 PFAIADATSLTEAGYVGEDVENILLKLIQAADYNVERAER 177

HSP 2 Score: 46.2098 bits (108), Expect = 1.206e-14
Identity = 26/56 (46.43%), Postives = 35/56 (62.50%), Query Frame = 3 
Query:  174 GENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            GEN    R VSGEG+   LLK+++ T  +VPP   G RK P  E I I+TK++ F+
Sbjct:  192 GENVSITRDVSGEGVQQALLKIIEGTVASVPP--QGGRKHPNQEMIQIDTKNILFI 245
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_STRPZ (ATP-dependent Clp protease ATP-binding subunit clpX OS=Streptococcus pyogenes serotype M49 (strain NZ131) GN=clpX PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.206e-14
Identity = 24/40 (60.00%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF I DAT+LT+AGYVGED E++L +L+ AAD++VE  +R
Sbjct:  138 PFAIADATSLTEAGYVGEDVENILLKLIQAADYNVERAER 177

HSP 2 Score: 46.2098 bits (108), Expect = 1.206e-14
Identity = 26/56 (46.43%), Postives = 35/56 (62.50%), Query Frame = 3 
Query:  174 GENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            GEN    R VSGEG+   LLK+++ T  +VPP   G RK P  E I I+TK++ F+
Sbjct:  192 GENVSITRDVSGEGVQQALLKIIEGTVASVPP--QGGRKHPNQEMIQIDTKNILFI 245
The following BLAST results are available for this feature:
BLAST of EY728351 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
CLPX_STRT17.142e-1560.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_THET87.146e-1566.67ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_THET27.146e-1566.67ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_HALOH9.275e-1560.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_STRPS9.278e-1565.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_STRPJ9.278e-1565.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_STRGC9.278e-1565.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_STRU01.206e-1460.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_STRS71.206e-1460.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_STRPZ1.206e-1460.00ATP-dependent Clp protease ATP-binding subunit clp... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-002-F01-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY728351 ID=EY728351; Name=EY728351; organism=Citrus sinensis; type=EST; length=811bp
CCACGCGTCCGGCCATTTACCATCACTGATGCAACAGCTTTAACGCAGGC
TGGGTATGTTGGAGAAGACTCGGAATCACTATTGTACCAGTTGTTATCGG
CTGCTGATTTCCACGTGGAAACACCTCAGAGACGGAATCGTCTACATTGA
TTAAGTTGAAAAAAATATTGAAAGGTGAAAATCCAAAAAATGGCAGAAAA
GTTTCGGGCGAAGGAATCTCCACAGGCTTACTCAAAATGCTAAAAAGGAC
TGGCATTAATGTTCCCCCACCTGATAACGGAACTCGGAAACCTCCCCCTT
GGGAAAACATTCCAATTGAAACCAAAGACCTGCCTTTCCTATCGTTGTGG
GGCCCTTCGCTGCTTTCCGACAAGCCGACTCCCACAAACCCCCCGAGCCC
TCTGTCCGCGGTCGGGCGCCTGCTCTCCCCCACATGCCAACGCCTTGTCT
GCGGGTACGCCGGTGGCATCCCCGTTGTGTGTCGCTGGATAGGGCAGGCG
TTCGGGTGTTGATGTGCCTGGTCCGGGGGCGCGCGCACGGCCCCTCTACG
TCGCAACGTCCTCTTGTGACTGGCGTCTGCACGCAGTGATTCGTAGTCAT
CCGCAAAGGTGGGGTTTGGGCTTCGGGGCGTGCGGGCACGAGGAGGTGGT
AGCTGTCCCCGGGGTTATCCGGTGTGTGGCGCCCATACGGTCTTCTCCCC
CGGAGCCGATGTGTGTGGCTGAGGATTATCTACTTCTCCGTGTCCCGACC
GCCGGCGTGTACTTGATCTGAGTCGGCGGATGCCACCCCAGGTCTCTCCG
CGCTCCAGGCA
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