EY728351

Overview
NameEY728351
Unique NameEY728351
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length811
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_STREM (ATP-dependent Clp protease ATP-binding subunit clpX OS=Streptococcus equi subsp. zooepidemicus (strain MGCS10565) GN=clpX PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.206e-14
Identity = 24/40 (60.00%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF I DAT+LT+AGYVGED E++L +L+ AAD++VE  +R
Sbjct:  138 PFAIADATSLTEAGYVGEDVENILLKLIQAADYNVERAER 177

HSP 2 Score: 46.2098 bits (108), Expect = 1.206e-14
Identity = 26/56 (46.43%), Postives = 35/56 (62.50%), Query Frame = 3 
Query:  174 GENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            GEN    R VSGEG+   LLK+++ T  +VPP   G RK P  E I I+TK++ F+
Sbjct:  192 GENVSITRDVSGEGVQQALLKIIEGTVASVPP--QGGRKHPNQEMIQIDTKNILFI 245
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_GLOVI (ATP-dependent Clp protease ATP-binding subunit clpX OS=Gloeobacter violaceus GN=clpX PE=3 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 1.564e-14
Identity = 25/40 (62.50%), Postives = 29/40 (72.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF I DAT LT+AGYVGED E++L +LL  AD  VE  QR
Sbjct:  153 PFAIADATTLTEAGYVGEDVENILLRLLQVADGEVERAQR 192

HSP 2 Score: 48.521 bits (114), Expect = 1.564e-14
Identity = 28/55 (50.91%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            ENP   R VSGEG+   LLKML+ T  NVPP   G RK P  E I I+T ++ F+
Sbjct:  208 ENPSITRDVSGEGVQQALLKMLEGTVANVPP--QGGRKHPYQEYIQIDTANILFV 260
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_MYXXA (ATP-dependent Clp protease ATP-binding subunit clpX OS=Myxococcus xanthus GN=clpX PE=3 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 1.565e-14
Identity = 25/40 (62.50%), Postives = 30/40 (75.00%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PFTI DAT LT+AGYVGED E+++  LL AAD  +E  QR
Sbjct:  140 PFTIADATCLTEAGYVGEDVENIIVNLLQAADHDIERAQR 179

HSP 2 Score: 46.2098 bits (108), Expect = 1.565e-14
Identity = 24/55 (43.64%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            ENP   R VSGEG+   LLK+++ T  NVPP   G RK P  E + ++T ++ F+
Sbjct:  195 ENPSITRDVSGEGVQQALLKLIEGTVANVPP--KGGRKHPQQEFLQVDTTNILFI 247
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_STRE4 (ATP-dependent Clp protease ATP-binding subunit clpX OS=Streptococcus equi subsp. equi (strain 4047) GN=clpX PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.566e-14
Identity = 24/40 (60.00%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF I DAT+LT+AGYVGED E++L +L+ AAD++VE  +R
Sbjct:  138 PFAIADATSLTEAGYVGEDVENILLKLIQAADYNVERAER 177

HSP 2 Score: 45.8246 bits (107), Expect = 1.566e-14
Identity = 25/56 (44.64%), Postives = 35/56 (62.50%), Query Frame = 3 
Query:  174 GENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            GEN    R VSGEG+   LLK+++ T  +VPP   G RK P  E + I+TK++ F+
Sbjct:  192 GENVSITRDVSGEGVQQALLKIIEGTVASVPP--QGGRKHPNQEMVQIDTKNILFI 245
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_PROMM (ATP-dependent Clp protease ATP-binding subunit clpX OS=Prochlorococcus marinus (strain MIT 9313) GN=clpX PE=3 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 1.902e-14
Identity = 24/40 (60.00%), Postives = 29/40 (72.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF + DAT LT+AGYVGED E++L +LL  AD  VE  QR
Sbjct:  162 PFAVADATTLTEAGYVGEDVENILLRLLQKADMDVEHAQR 201

HSP 2 Score: 47.3654 bits (111), Expect = 1.902e-14
Identity = 27/55 (49.09%), Postives = 33/55 (60.00%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            ENP   R VSGEG+   LLKML+ T  NVPP   G RK P  + I I+T  + F+
Sbjct:  217 ENPSITRDVSGEGVQQALLKMLEGTVANVPP--QGGRKHPYQDCIQIDTSQILFI 269

HSP 3 Score: 20.7866 bits (42), Expect = 1.902e-14
Identity = 6/15 (40.00%), Postives = 12/15 (80.00%), Query Frame = 2 
Query:  119 KHLRDGIVYID*VEK 163
            +H + GI+Y+D ++K
Sbjct:  197 EHAQRGIIYVDEIDK 211
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_PROM3 (ATP-dependent Clp protease ATP-binding subunit clpX OS=Prochlorococcus marinus (strain MIT 9303) GN=clpX PE=3 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 1.902e-14
Identity = 24/40 (60.00%), Postives = 29/40 (72.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF + DAT LT+AGYVGED E++L +LL  AD  VE  QR
Sbjct:  162 PFAVADATTLTEAGYVGEDVENILLRLLQKADMDVEHAQR 201

HSP 2 Score: 47.3654 bits (111), Expect = 1.902e-14
Identity = 27/55 (49.09%), Postives = 33/55 (60.00%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            ENP   R VSGEG+   LLKML+ T  NVPP   G RK P  + I I+T  + F+
Sbjct:  217 ENPSITRDVSGEGVQQALLKMLEGTVANVPP--QGGRKHPYQDCIQIDTSQILFI 269

HSP 3 Score: 20.7866 bits (42), Expect = 1.902e-14
Identity = 6/15 (40.00%), Postives = 12/15 (80.00%), Query Frame = 2 
Query:  119 KHLRDGIVYID*VEK 163
            +H + GI+Y+D ++K
Sbjct:  197 EHAQRGIIYVDEIDK 211
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_SYNP6 (ATP-dependent Clp protease ATP-binding subunit clpX OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=clpX PE=3 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 2.030e-14
Identity = 23/40 (57.50%), Postives = 29/40 (72.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF + DAT LT+AGYVGED E++L +LL  AD  +E  QR
Sbjct:  165 PFAVADATTLTEAGYVGEDVENILLRLLQVADLDIEEAQR 204

HSP 2 Score: 48.1358 bits (113), Expect = 2.030e-14
Identity = 27/55 (49.09%), Postives = 35/55 (63.64%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            ENP   R VSGEG+   LLKML+ T  NVPP   G RK P  + I I+T+++ F+
Sbjct:  220 ENPSITRDVSGEGVQQALLKMLEGTIANVPP--QGGRKHPYQDCIQIDTRNILFI 272
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_SYNE7 (ATP-dependent Clp protease ATP-binding subunit clpX OS=Synechococcus elongatus (strain PCC 7942) GN=clpX PE=3 SV=2)

HSP 1 Score: 51.9878 bits (123), Expect = 2.030e-14
Identity = 23/40 (57.50%), Postives = 29/40 (72.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF + DAT LT+AGYVGED E++L +LL  AD  +E  QR
Sbjct:  165 PFAVADATTLTEAGYVGEDVENILLRLLQVADLDIEEAQR 204

HSP 2 Score: 48.1358 bits (113), Expect = 2.030e-14
Identity = 27/55 (49.09%), Postives = 35/55 (63.64%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            ENP   R VSGEG+   LLKML+ T  NVPP   G RK P  + I I+T+++ F+
Sbjct:  220 ENPSITRDVSGEGVQQALLKMLEGTIANVPP--QGGRKHPYQDCIQIDTRNILFI 272
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_SYNY3 (ATP-dependent Clp protease ATP-binding subunit clpX OS=Synechocystis sp. (strain PCC 6803) GN=clpX PE=3 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 2.031e-14
Identity = 24/40 (60.00%), Postives = 29/40 (72.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF + DAT LT+AGYVGED E++L +LL  AD  VE  QR
Sbjct:  161 PFAVADATTLTEAGYVGEDVENILLRLLQVADLDVEEAQR 200

HSP 2 Score: 47.7506 bits (112), Expect = 2.031e-14
Identity = 27/55 (49.09%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            ENP   R VSGEG+   LLKML+ T  NVPP   G RK P  + I I+T ++ F+
Sbjct:  216 ENPSITRDVSGEGVQQALLKMLEGTVANVPP--QGGRKHPYQDCIQIDTSNILFI 268
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_CYAP7 (ATP-dependent Clp protease ATP-binding subunit clpX OS=Cyanothece sp. (strain PCC 7424) GN=clpX PE=3 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 2.031e-14
Identity = 24/40 (60.00%), Postives = 29/40 (72.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF + DAT LT+AGYVGED E++L +LL  AD  VE  QR
Sbjct:  163 PFAVADATTLTEAGYVGEDVENILLRLLQVADLDVEEAQR 202

HSP 2 Score: 47.7506 bits (112), Expect = 2.031e-14
Identity = 27/55 (49.09%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            ENP   R VSGEG+   LLKML+ T  NVPP   G RK P  + I I+T ++ F+
Sbjct:  218 ENPSITRDVSGEGVQQALLKMLEGTVANVPP--QGGRKHPYQDCIQIDTSNILFI 270
The following BLAST results are available for this feature:
BLAST of EY728351 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
CLPX_STREM1.206e-1460.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_GLOVI1.564e-1462.50ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_MYXXA1.565e-1462.50ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_STRE41.566e-1460.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_PROMM1.902e-1460.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_PROM31.902e-1460.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_SYNP62.030e-1457.50ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_SYNE72.030e-1457.50ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_SYNY32.031e-1460.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_CYAP72.031e-1460.00ATP-dependent Clp protease ATP-binding subunit clp... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-002-F01-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY728351 ID=EY728351; Name=EY728351; organism=Citrus sinensis; type=EST; length=811bp
CCACGCGTCCGGCCATTTACCATCACTGATGCAACAGCTTTAACGCAGGC
TGGGTATGTTGGAGAAGACTCGGAATCACTATTGTACCAGTTGTTATCGG
CTGCTGATTTCCACGTGGAAACACCTCAGAGACGGAATCGTCTACATTGA
TTAAGTTGAAAAAAATATTGAAAGGTGAAAATCCAAAAAATGGCAGAAAA
GTTTCGGGCGAAGGAATCTCCACAGGCTTACTCAAAATGCTAAAAAGGAC
TGGCATTAATGTTCCCCCACCTGATAACGGAACTCGGAAACCTCCCCCTT
GGGAAAACATTCCAATTGAAACCAAAGACCTGCCTTTCCTATCGTTGTGG
GGCCCTTCGCTGCTTTCCGACAAGCCGACTCCCACAAACCCCCCGAGCCC
TCTGTCCGCGGTCGGGCGCCTGCTCTCCCCCACATGCCAACGCCTTGTCT
GCGGGTACGCCGGTGGCATCCCCGTTGTGTGTCGCTGGATAGGGCAGGCG
TTCGGGTGTTGATGTGCCTGGTCCGGGGGCGCGCGCACGGCCCCTCTACG
TCGCAACGTCCTCTTGTGACTGGCGTCTGCACGCAGTGATTCGTAGTCAT
CCGCAAAGGTGGGGTTTGGGCTTCGGGGCGTGCGGGCACGAGGAGGTGGT
AGCTGTCCCCGGGGTTATCCGGTGTGTGGCGCCCATACGGTCTTCTCCCC
CGGAGCCGATGTGTGTGGCTGAGGATTATCTACTTCTCCGTGTCCCGACC
GCCGGCGTGTACTTGATCTGAGTCGGCGGATGCCACCCCAGGTCTCTCCG
CGCTCCAGGCA
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