EY728351

Overview
NameEY728351
Unique NameEY728351
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length811
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_XANP2 (ATP-dependent Clp protease ATP-binding subunit clpX OS=Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) GN=clpX PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 4.459e-14
Identity = 25/40 (62.50%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PFT+ DAT LT+AGYVGED E+++ +LL AAD++VE  QR
Sbjct:  138 PFTMADATTLTEAGYVGEDVENIILKLLQAADYNVERAQR 177

HSP 2 Score: 42.743 bits (99), Expect = 4.459e-14
Identity = 22/55 (40.00%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            +NP   R VSGEG+   LLK+++ T  +VPP   G RK P  E + ++T ++ F+
Sbjct:  193 DNPSITRDVSGEGVQQALLKIMEGTVASVPP--QGGRKHPQQEFLQVDTTNILFI 245
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_RHOCS (ATP-dependent Clp protease ATP-binding subunit clpX OS=Rhodospirillum centenum (strain ATCC 51521 / SW) GN=clpX PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 4.459e-14
Identity = 25/40 (62.50%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PFT+ DAT LT+AGYVGED E+++ +LL AAD++VE  QR
Sbjct:  138 PFTMADATTLTEAGYVGEDVENIILKLLQAADYNVERAQR 177

HSP 2 Score: 42.743 bits (99), Expect = 4.459e-14
Identity = 22/55 (40.00%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            +NP   R VSGEG+   LLK+++ T  +VPP   G RK P  E + ++T ++ F+
Sbjct:  193 DNPSITRDVSGEGVQQALLKIMEGTVASVPP--QGGRKHPQQEFLQVDTTNILFI 245
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_BRAJA (ATP-dependent Clp protease ATP-binding subunit clpX OS=Bradyrhizobium japonicum GN=clpX PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 4.459e-14
Identity = 25/40 (62.50%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PFT+ DAT LT+AGYVGED E+++ +LL AAD++VE  QR
Sbjct:  138 PFTMADATTLTEAGYVGEDVENIILKLLQAADYNVERAQR 177

HSP 2 Score: 42.743 bits (99), Expect = 4.459e-14
Identity = 22/55 (40.00%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            +NP   R VSGEG+   LLK+++ T  +VPP   G RK P  E + ++T ++ F+
Sbjct:  193 DNPSITRDVSGEGVQQALLKIMEGTVASVPP--QGGRKHPQQEFLQVDTTNILFI 245
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_AZOC5 (ATP-dependent Clp protease ATP-binding subunit clpX OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) GN=clpX PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 4.459e-14
Identity = 25/40 (62.50%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PFT+ DAT LT+AGYVGED E+++ +LL AAD++VE  QR
Sbjct:  138 PFTMADATTLTEAGYVGEDVENIILKLLQAADYNVERAQR 177

HSP 2 Score: 42.743 bits (99), Expect = 4.459e-14
Identity = 22/55 (40.00%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            +NP   R VSGEG+   LLK+++ T  +VPP   G RK P  E + ++T ++ F+
Sbjct:  193 DNPSITRDVSGEGVQQALLKIMEGTVASVPP--QGGRKHPQQEFLQVDTTNILFI 245
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_SPHAL (ATP-dependent Clp protease ATP-binding subunit clpX OS=Sphingopyxis alaskensis GN=clpX PE=3 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 4.459e-14
Identity = 23/40 (57.50%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PFT+ DAT LT+AGYVGED E+++ +LL ++D++VE  QR
Sbjct:  139 PFTMADATTLTEAGYVGEDVENIILKLLQSSDYNVEKAQR 178

HSP 2 Score: 45.0542 bits (105), Expect = 4.459e-14
Identity = 23/56 (41.07%), Postives = 35/56 (62.50%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFLS 344
            ENP   R VSGEG+   LLK+++ T  +VPP   G RK P  E + ++T ++ F++
Sbjct:  194 ENPSITRDVSGEGVQQALLKLMEGTTASVPP--QGGRKHPQQEFLQVDTTNILFIA 247
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_GRABC (ATP-dependent Clp protease ATP-binding subunit clpX OS=Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) GN=clpX PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 4.460e-14
Identity = 25/40 (62.50%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PFT+ DAT LT+AGYVGED E+++ +LL AAD++VE  QR
Sbjct:  138 PFTMADATTLTEAGYVGEDVENIILKLLQAADYNVERAQR 177

HSP 2 Score: 42.743 bits (99), Expect = 4.460e-14
Identity = 22/55 (40.00%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            +NP   R VSGEG+   LLK+++ T  +VPP   G RK P  E + ++T ++ F+
Sbjct:  193 DNPSITRDVSGEGVQQALLKIMEGTVASVPP--QGGRKHPQQEFLQVDTTNILFI 245
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_GLUDA (ATP-dependent Clp protease ATP-binding subunit clpX OS=Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5) GN=clpX PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 4.460e-14
Identity = 25/40 (62.50%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PFT+ DAT LT+AGYVGED E+++ +LL AAD++VE  QR
Sbjct:  137 PFTMADATTLTEAGYVGEDVENIILKLLQAADYNVERAQR 176

HSP 2 Score: 42.743 bits (99), Expect = 4.460e-14
Identity = 22/55 (40.00%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            +NP   R VSGEG+   LLK+++ T  +VPP   G RK P  E + ++T ++ F+
Sbjct:  192 DNPSITRDVSGEGVQQALLKIMEGTVASVPP--QGGRKHPQQEFLQVDTTNMLFI 244
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_ACICJ (ATP-dependent Clp protease ATP-binding subunit clpX OS=Acidiphilium cryptum (strain JF-5) GN=clpX PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 4.460e-14
Identity = 25/40 (62.50%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PFT+ DAT LT+AGYVGED E+++ +LL AAD++VE  QR
Sbjct:  137 PFTMADATTLTEAGYVGEDVENIILKLLQAADYNVERAQR 176

HSP 2 Score: 42.743 bits (99), Expect = 4.460e-14
Identity = 22/55 (40.00%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            +NP   R VSGEG+   LLK+++ T  +VPP   G RK P  E + ++T ++ F+
Sbjct:  192 DNPSITRDVSGEGVQQALLKIMEGTVASVPP--QGGRKHPQQEFLQVDTTNILFI 244
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_LACLM (ATP-dependent Clp protease ATP-binding subunit clpX OS=Lactococcus lactis subsp. cremoris (strain MG1363) GN=clpX PE=3 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 4.463e-14
Identity = 24/40 (60.00%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF I DAT+LT+AGYVGED E++L +LL A+DF++E  +R
Sbjct:  136 PFAIADATSLTEAGYVGEDVENILLKLLQASDFNIERAER 175

HSP 2 Score: 43.8986 bits (102), Expect = 4.463e-14
Identity = 25/55 (45.45%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            EN    R VSGEG+   LLK+++ T  +VPP   G RK P  E I I+TK++ F+
Sbjct:  191 ENVSITRDVSGEGVQQALLKIIEGTVASVPP--QGGRKHPNQEMIQIDTKNILFI 243
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_LACLA (ATP-dependent Clp protease ATP-binding subunit clpX OS=Lactococcus lactis subsp. lactis GN=clpX PE=3 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 4.463e-14
Identity = 24/40 (60.00%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF I DAT+LT+AGYVGED E++L +LL A+DF++E  +R
Sbjct:  136 PFAIADATSLTEAGYVGEDVENILLKLLQASDFNIERAER 175

HSP 2 Score: 43.8986 bits (102), Expect = 4.463e-14
Identity = 25/55 (45.45%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            EN    R VSGEG+   LLK+++ T  +VPP   G RK P  E I I+TK++ F+
Sbjct:  191 ENVSITRDVSGEGVQQALLKIIEGTVASVPP--QGGRKHPNQEMIQIDTKNILFI 243
The following BLAST results are available for this feature:
BLAST of EY728351 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
CLPX_XANP24.459e-1462.50ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_RHOCS4.459e-1462.50ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_BRAJA4.459e-1462.50ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_AZOC54.459e-1462.50ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_SPHAL4.459e-1457.50ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_GRABC4.460e-1462.50ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_GLUDA4.460e-1462.50ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_ACICJ4.460e-1462.50ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_LACLM4.463e-1460.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_LACLA4.463e-1460.00ATP-dependent Clp protease ATP-binding subunit clp... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-002-F01-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY728351 ID=EY728351; Name=EY728351; organism=Citrus sinensis; type=EST; length=811bp
CCACGCGTCCGGCCATTTACCATCACTGATGCAACAGCTTTAACGCAGGC
TGGGTATGTTGGAGAAGACTCGGAATCACTATTGTACCAGTTGTTATCGG
CTGCTGATTTCCACGTGGAAACACCTCAGAGACGGAATCGTCTACATTGA
TTAAGTTGAAAAAAATATTGAAAGGTGAAAATCCAAAAAATGGCAGAAAA
GTTTCGGGCGAAGGAATCTCCACAGGCTTACTCAAAATGCTAAAAAGGAC
TGGCATTAATGTTCCCCCACCTGATAACGGAACTCGGAAACCTCCCCCTT
GGGAAAACATTCCAATTGAAACCAAAGACCTGCCTTTCCTATCGTTGTGG
GGCCCTTCGCTGCTTTCCGACAAGCCGACTCCCACAAACCCCCCGAGCCC
TCTGTCCGCGGTCGGGCGCCTGCTCTCCCCCACATGCCAACGCCTTGTCT
GCGGGTACGCCGGTGGCATCCCCGTTGTGTGTCGCTGGATAGGGCAGGCG
TTCGGGTGTTGATGTGCCTGGTCCGGGGGCGCGCGCACGGCCCCTCTACG
TCGCAACGTCCTCTTGTGACTGGCGTCTGCACGCAGTGATTCGTAGTCAT
CCGCAAAGGTGGGGTTTGGGCTTCGGGGCGTGCGGGCACGAGGAGGTGGT
AGCTGTCCCCGGGGTTATCCGGTGTGTGGCGCCCATACGGTCTTCTCCCC
CGGAGCCGATGTGTGTGGCTGAGGATTATCTACTTCTCCGTGTCCCGACC
GCCGGCGTGTACTTGATCTGAGTCGGCGGATGCCACCCCAGGTCTCTCCG
CGCTCCAGGCA
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