EY754248

Overview
NameEY754248
Unique NameEY754248
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length976
Libraries
Library NameType
Sweet orange leaf, field plant Acdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY754248 vs. ExPASy Swiss-Prot
Match: HIS7_CUPTR (Imidazoleglycerol-phosphate dehydratase OS=Cupriavidus taiwanensis (strain R1 / LMG 19424) GN=hisB PE=3 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 5.782e-41
Identity = 71/141 (50.35%), Postives = 94/141 (66.67%), Query Frame = 3 
Query:  351 RIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRPHLNYDLQIPTQRVXTYDTQLVEHFXQSLV 773
            R+ EV R T ET + V +N+DGSG    ++ +PFLDHMLDQ+A HG+FD+ V ATGD HIDDHHT EDVG+ +G A+ +A+GD+KGI R+G    PLDE L  V +D SGRP L + +     RV ++D  L   F +  V
Sbjct:    2 RVAEVTRNTSETQIRVSLNLDGSGRQKLASGVPFLDHMLDQIARHGMFDLEVEATGDTHIDDHHTVEDVGITLGQAVARAIGDKKGITRYGHSYVPLDECLSRVVIDFSGRPGLEFHVPFTRARVGSFDVDLTIEFFRGFV 142

HSP 2 Score: 38.1206 bits (87), Expect = 5.782e-41
Identity = 23/57 (40.35%), Postives = 29/57 (50.88%), Query Frame = 2 
Query:  758 FXVFGDTSGMTLHIRQLAGKNXHHIIEGTFKLL-LGLXDKHRYDPPSRGTIPAQKGS 925
            F  F + +G+TLHI  L G N HH  E  FK     L      DP +  TIP+ KG+
Sbjct:  138 FRGFVNHAGVTLHIDNLRGINAHHQCETVFKAFGRALRMAVELDPRAANTIPSTKGT 194
[Close]
BLAST of EY754248 vs. ExPASy Swiss-Prot
Match: HIS7_NEIMF (Imidazoleglycerol-phosphate dehydratase OS=Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / FAM18) GN=hisB PE=3 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 7.476e-41
Identity = 76/176 (43.18%), Postives = 107/176 (60.80%), Query Frame = 3 
Query:  246 RRMNSLTTPRASLNPDGDSKHNNGSASTSSAVDSGRIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRPHLNYDLQIPTQRVXTYDTQLVEHFXQSLV 773
            R+    T P  +    G ++     A T+SA     +  V R T ET ++V IN+DGSG +   T +PFL+HM+DQ+A HG+ D+ +   GD+HIDDHHT ED+G+ +G A+ QALGD+KGI R+G    PLDEAL  V +DLSGRP L Y+++     +  +D  L E F   +V
Sbjct:   78 RKHRERTVPETAAESTGTAETQ--IAETASAAGCRSV-TVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDIDISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLSGRPGLVYNIEFTRALIGRFDVDLFEEFFHGIV 250

HSP 2 Score: 38.1206 bits (87), Expect = 7.476e-41
Identity = 22/50 (44.00%), Postives = 26/50 (52.00%), Query Frame = 2 
Query:  779 SGMTLHIRQLAGKNXHHIIEGTFKLL-LGLXDKHRYDPPSRGTIPAQKGS 925
            S MTLHI  L+GKN HH  E  FK     L     +DP   G  P+ KG+
Sbjct:  253 SMMTLHIDNLSGKNAHHQAETVFKAFGRALRMAVEHDPRMAGQTPSTKGT 302
[Close]
BLAST of EY754248 vs. ExPASy Swiss-Prot
Match: HIS7_NEIMB (Imidazoleglycerol-phosphate dehydratase OS=Neisseria meningitidis serogroup B GN=hisB PE=3 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 7.476e-41
Identity = 76/176 (43.18%), Postives = 107/176 (60.80%), Query Frame = 3 
Query:  246 RRMNSLTTPRASLNPDGDSKHNNGSASTSSAVDSGRIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRPHLNYDLQIPTQRVXTYDTQLVEHFXQSLV 773
            R+    T P  +    G ++     A T+SA     +  V R T ET ++V IN+DGSG +   T +PFL+HM+DQ+A HG+ D+ +   GD+HIDDHHT ED+G+ +G A+ QALGD+KGI R+G    PLDEAL  V +DLSGRP L Y+++     +  +D  L E F   +V
Sbjct:   78 RKHRERTVPETAAESTGTAETQ--IAETASAAGCRSV-TVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDIDISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLSGRPGLVYNIEFTRALIGRFDVDLFEEFFHGIV 250

HSP 2 Score: 38.1206 bits (87), Expect = 7.476e-41
Identity = 22/50 (44.00%), Postives = 26/50 (52.00%), Query Frame = 2 
Query:  779 SGMTLHIRQLAGKNXHHIIEGTFKLL-LGLXDKHRYDPPSRGTIPAQKGS 925
            S MTLHI  L+GKN HH  E  FK     L     +DP   G  P+ KG+
Sbjct:  253 SMMTLHIDNLSGKNAHHQAETVFKAFGRALRMAVEHDPRMAGQTPSTKGT 302
[Close]
BLAST of EY754248 vs. ExPASy Swiss-Prot
Match: HIS7_NEIMA (Imidazoleglycerol-phosphate dehydratase OS=Neisseria meningitidis serogroup A GN=hisB PE=3 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 7.476e-41
Identity = 76/176 (43.18%), Postives = 107/176 (60.80%), Query Frame = 3 
Query:  246 RRMNSLTTPRASLNPDGDSKHNNGSASTSSAVDSGRIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRPHLNYDLQIPTQRVXTYDTQLVEHFXQSLV 773
            R+    T P  +    G ++     A T+SA     +  V R T ET ++V IN+DGSG +   T +PFL+HM+DQ+A HG+ D+ +   GD+HIDDHHT ED+G+ +G A+ QALGD+KGI R+G    PLDEAL  V +DLSGRP L Y+++     +  +D  L E F   +V
Sbjct:   78 RKHRERTVPETAAESTGTAETQ--IAETASAAGCRSV-TVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDIDISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLSGRPGLVYNIEFTRALIGRFDVDLFEEFFHGIV 250

HSP 2 Score: 38.1206 bits (87), Expect = 7.476e-41
Identity = 22/50 (44.00%), Postives = 26/50 (52.00%), Query Frame = 2 
Query:  779 SGMTLHIRQLAGKNXHHIIEGTFKLL-LGLXDKHRYDPPSRGTIPAQKGS 925
            S MTLHI  L+GKN HH  E  FK     L     +DP   G  P+ KG+
Sbjct:  253 SMMTLHIDNLSGKNAHHQAETVFKAFGRALRMAVEHDPRMAGQTPSTKGT 302
[Close]
BLAST of EY754248 vs. ExPASy Swiss-Prot
Match: HIS7_NEIM0 (Imidazoleglycerol-phosphate dehydratase OS=Neisseria meningitidis serogroup C (strain 053442) GN=hisB PE=3 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 7.476e-41
Identity = 76/176 (43.18%), Postives = 107/176 (60.80%), Query Frame = 3 
Query:  246 RRMNSLTTPRASLNPDGDSKHNNGSASTSSAVDSGRIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRPHLNYDLQIPTQRVXTYDTQLVEHFXQSLV 773
            R+    T P  +    G ++     A T+SA     +  V R T ET ++V IN+DGSG +   T +PFL+HM+DQ+A HG+ D+ +   GD+HIDDHHT ED+G+ +G A+ QALGD+KGI R+G    PLDEAL  V +DLSGRP L Y+++     +  +D  L E F   +V
Sbjct:   78 RKHRERTVPETAAESTGTAETQ--IAETASAAGCRSV-TVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDIDISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLSGRPGLVYNIEFTRALIGRFDVDLFEEFFHGIV 250

HSP 2 Score: 38.1206 bits (87), Expect = 7.476e-41
Identity = 22/50 (44.00%), Postives = 26/50 (52.00%), Query Frame = 2 
Query:  779 SGMTLHIRQLAGKNXHHIIEGTFKLL-LGLXDKHRYDPPSRGTIPAQKGS 925
            S MTLHI  L+GKN HH  E  FK     L     +DP   G  P+ KG+
Sbjct:  253 SMMTLHIDNLSGKNAHHQAETVFKAFGRALRMAVEHDPRMAGQTPSTKGT 302
[Close]
BLAST of EY754248 vs. ExPASy Swiss-Prot
Match: HIS7_NEIG1 (Imidazoleglycerol-phosphate dehydratase OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=hisB PE=3 SV=2)

HSP 1 Score: 150.984 bits (380), Expect = 7.477e-41
Identity = 76/176 (43.18%), Postives = 107/176 (60.80%), Query Frame = 3 
Query:  246 RRMNSLTTPRASLNPDGDSKHNNGSASTSSAVDSGRIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRPHLNYDLQIPTQRVXTYDTQLVEHFXQSLV 773
            R+    T P  +    G ++     A T+SA     +  V R T ET ++V IN+DGSG +   T +PFL+HM+DQ+A HG+ D+ +   GD+HIDDHHT ED+G+ +G A+ QALGD+KGI R+G    PLDEAL  V +DLSGRP L Y+++     +  +D  L E F   +V
Sbjct:   76 RKHRERTVPETAAESTGTAETR--IAETASAAGCRSV-TVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDIDISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLSGRPGLVYNIEFTRTLIGRFDVDLFEEFFHGIV 248

HSP 2 Score: 38.1206 bits (87), Expect = 7.477e-41
Identity = 22/50 (44.00%), Postives = 26/50 (52.00%), Query Frame = 2 
Query:  779 SGMTLHIRQLAGKNXHHIIEGTFKLL-LGLXDKHRYDPPSRGTIPAQKGS 925
            S MTLHI  L+GKN HH  E  FK     L     +DP   G  P+ KG+
Sbjct:  251 SMMTLHIDNLSGKNAHHQAETVFKAFGRALRMAVEHDPRMAGQTPSTKGT 300
[Close]
BLAST of EY754248 vs. ExPASy Swiss-Prot
Match: HIS7_AZOC5 (Imidazoleglycerol-phosphate dehydratase OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) GN=hisB PE=3 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 7.532e-41
Identity = 67/142 (47.18%), Postives = 93/142 (65.49%), Query Frame = 3 
Query:  351 RIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRPHLNYDLQIPTQRVXTYDTQLVEHFXQSLVT 776
            R  E+ R TKET V + +N+DG+G A  +T I F DHMLD LA H  FD+ V A GD+H+D HHT EDVG+ +G A+ +ALGD +GI R+ D   P+DE L  V+LD+SGRP L +  +    ++  +DT+LV  F Q+  +
Sbjct:    2 RQAEIVRETKETKVRLAVNLDGTGRASIATGIGFFDHMLDLLARHARFDLEVEAKGDLHVDFHHTTEDVGIVLGQAIRRALGDMRGITRYADLHLPMDETLTRVALDISGRPFLVFRTEFKVGKIGEFDTELVREFFQAFAS 143

HSP 2 Score: 46.2098 bits (108), Expect = 7.532e-41
Identity = 24/57 (42.11%), Postives = 30/57 (52.63%), Query Frame = 2 
Query:  758 FXVFGDTSGMTLHIRQLAGKNXHHIIEGTFK-LLLGLXDKHRYDPPSRGTIPAQKGS 925
            F  F   +G+TLH+  L G N HHI E  FK L   L      DP + G IP+ KG+
Sbjct:  138 FQAFASNAGVTLHVETLYGDNAHHIAESCFKGLARALRAAVAIDPAAAGEIPSTKGA 194
[Close]
BLAST of EY754248 vs. ExPASy Swiss-Prot
Match: HIS7_RHOP2 (Imidazoleglycerol-phosphate dehydratase OS=Rhodopseudomonas palustris (strain HaA2) GN=hisB PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 9.814e-41
Identity = 65/139 (46.76%), Postives = 95/139 (68.35%), Query Frame = 3 
Query:  351 RIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRPHLNYDLQIPTQRVXTYDTQLVEHFXQS 767
            R   +KR TKET++ V +N+DG+GV++++T I F DHMLD LA H   D+ V+A GD+H+D HHT EDVG+A+G A+ QALG+  GI R+     P+DE L  V +D+SGRP L +  + P  ++  +DT+LV  + Q+
Sbjct:    2 RTATIKRKTKETDIEVTVNLDGAGVSNAATGIGFFDHMLDLLAKHSRIDITVKAVGDLHVDFHHTTEDVGIALGQAVRQALGNMAGIIRYASMLMPMDETLTRVVIDVSGRPFLVFKAEFPRDKIGEFDTELVREWFQA 140

HSP 2 Score: 45.0542 bits (105), Expect = 9.814e-41
Identity = 23/56 (41.07%), Postives = 30/56 (53.57%), Query Frame = 2 
Query:  758 FXVFGDTSGMTLHIRQLAGKNXHHIIEGTFK-LLLGLXDKHRYDPPSRGTIPAQKG 922
            F  F   +G+TLH+  L G+N HHI E  FK L   L      DP + G +P+ KG
Sbjct:  138 FQAFAMNAGVTLHVETLYGENSHHIAESCFKGLARALRAAVAIDPQAAGEVPSTKG 193
[Close]
BLAST of EY754248 vs. ExPASy Swiss-Prot
Match: HIS7_BURP1 (Imidazoleglycerol-phosphate dehydratase OS=Burkholderia pseudomallei (strain 1710b) GN=hisB PE=3 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 9.817e-41
Identity = 72/141 (51.06%), Postives = 95/141 (67.38%), Query Frame = 3 
Query:  351 RIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRPHLNYDLQIPTQRVXTYDTQLVEHFXQSLV 773
            R+ +V R T ET +SVKI++DG+G    +T +PFLDHMLDQ+A HGL D+ + A GD HIDDHHT EDVG+ +G A+ +A+GDRKGI R+G    PLDEAL  V +D SGRP L + +     R+ T+D  L   F +  V
Sbjct:    2 RVAQVVRNTSETQISVKIDLDGTGRQKLATGVPFLDHMLDQIARHGLVDLDIEANGDTHIDDHHTVEDVGITLGQAVAKAVGDRKGIRRYGHSYVPLDEALSRVVIDFSGRPGLEFHVPFTRARIGTFDVDLSIEFFRGFV 142

HSP 2 Score: 37.7354 bits (86), Expect = 9.817e-41
Identity = 23/57 (40.35%), Postives = 29/57 (50.88%), Query Frame = 2 
Query:  758 FXVFGDTSGMTLHIRQLAGKNXHHIIEGTFKLL-LGLXDKHRYDPPSRGTIPAQKGS 925
            F  F + +G+TLHI  L G N HH +E  FK     L      D  + G IP+ KGS
Sbjct:  138 FRGFVNHAGVTLHIDNLRGVNAHHQLETVFKAFGRALRMAVELDERAAGQIPSTKGS 194
[Close]
BLAST of EY754248 vs. ExPASy Swiss-Prot
Match: HIS7_RALEJ (Imidazoleglycerol-phosphate dehydratase OS=Ralstonia eutropha (strain JMP134) GN=hisB PE=3 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 1.279e-40
Identity = 70/141 (49.65%), Postives = 94/141 (66.67%), Query Frame = 3 
Query:  351 RIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRPHLNYDLQIPTQRVXTYDTQLVEHFXQSLV 773
            R+ EV R T ET + V +N+DG+G    ++ +PFLDHMLDQ+A HG+FD+ V ATGD HIDDHHT EDVG+ +G A+ +A+GD+KGI R+G    PLDE L  V +D SGRP L + +     RV ++D  L   F +  V
Sbjct:    2 RVAEVTRNTSETQIRVSLNLDGTGRQKLASGVPFLDHMLDQIARHGMFDLEVEATGDTHIDDHHTVEDVGITLGQAVAKAIGDKKGITRYGHSYVPLDECLSRVVIDFSGRPGLEFHVPFTRARVGSFDVDLTIEFFRGFV 142

HSP 2 Score: 38.1206 bits (87), Expect = 1.279e-40
Identity = 23/57 (40.35%), Postives = 29/57 (50.88%), Query Frame = 2 
Query:  758 FXVFGDTSGMTLHIRQLAGKNXHHIIEGTFKLL-LGLXDKHRYDPPSRGTIPAQKGS 925
            F  F + +G+TLHI  L G N HH  E  FK     L      DP +  TIP+ KG+
Sbjct:  138 FRGFVNHAGVTLHIDNLRGINAHHQCETVFKAFGRALRMAVELDPRAANTIPSTKGT 194
The following BLAST results are available for this feature:
BLAST of EY754248 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
HIS7_CUPTR5.782e-4150.35Imidazoleglycerol-phosphate dehydratase OS=Cupriav... [more]
HIS7_NEIMF7.476e-4143.18Imidazoleglycerol-phosphate dehydratase OS=Neisser... [more]
HIS7_NEIMB7.476e-4143.18Imidazoleglycerol-phosphate dehydratase OS=Neisser... [more]
HIS7_NEIMA7.476e-4143.18Imidazoleglycerol-phosphate dehydratase OS=Neisser... [more]
HIS7_NEIM07.476e-4143.18Imidazoleglycerol-phosphate dehydratase OS=Neisser... [more]
HIS7_NEIG17.477e-4143.18Imidazoleglycerol-phosphate dehydratase OS=Neisser... [more]
HIS7_AZOC57.532e-4147.18Imidazoleglycerol-phosphate dehydratase OS=Azorhiz... [more]
HIS7_RHOP29.814e-4146.76Imidazoleglycerol-phosphate dehydratase OS=Rhodops... [more]
HIS7_BURP19.817e-4151.06Imidazoleglycerol-phosphate dehydratase OS=Burkhol... [more]
HIS7_RALEJ1.279e-4049.65Imidazoleglycerol-phosphate dehydratase OS=Ralston... [more]

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Properties
Property NameValue
Genbank descriptionCS12-C1-001-012-D11-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY754248 ID=EY754248; Name=EY754248; organism=Citrus sinensis; type=EST; length=976bp
GCCGGNATTCCAAGCCCGACCCACGCGTCCGAGAAAAAACTTTGCGTTTT
AGCAAAAGCCGTGTTGGCGTTGCTACTCTCTCTGCGACATTGGAAACGAT
AGTGTGTAAAATGGAGCTCTCGGTCCCAGCTCGCCTTGTTCGCCTTTCAA
TCTCGTCCACGTCCTCACGCCGGCTCCTCGTTAAACCAAAAACCAATCTC
TCTCGTAAACTTCTCCCCATCTCCACACAATACTGCTCCTCAACGCGCCG
CATGAACTCCCTCACAACACCACGCGCCTCTCTAAACCCAGACGGCGACA
GCAAACACAACAACGGGTCCGCCTCAACTTCCTCTGCCGTCGATTCTGGT
CGAATTGGGGAAGTGAAAAGAGTCACGAAAGAGACCAACGTTTCTGTGAA
GATTAACGTCGATGGCTCTGGCGTTGCTGATTCATCCACTTGCATTCCCT
TCCTAGATCACATGCTTGATCAACTGGCTTCGCACGGGCTATTTGATGTG
CACGTGAGGGCAACCGGAGACATTCACATTGATGATCATCATACCAATGA
AGACGTTGGGCTTGCCATTGGCACGGCATTGCTGCAGGCTCTTGGTGATA
GGAAAGGAATTAATCGGTTTGGTGATTTTACAGCTCCTCTTGATGAAGCT
TTGATTCATGTTTCATTGGATTTATCTGGACGGCCACATTTAAATTATGA
TTTGCAAATTCCCACACAGAGAGTGGNAACTTACGACACTCAATTGGTGG
AGCACTTTTTNCAGTCTTTGGTGACACTTCTGGAATGACTCTTCATATTC
GACAGCTNGCTGGAAAAAATNCTCATCACATTATTGAGGGCACCTTCAAG
CTTTTGCTAGGGCTCTNCGACAAGCACAGATATGATCCACCGTCGCGTGG
AACTATACCAGCTCAAAAGGGGTCTACTCGATCTTGATCTTACGGGATCA
TGGACGGGGGATGGGTTCTNATGTAC
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