EY754217

Overview
NameEY754217
Unique NameEY754217
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length861
Libraries
Library NameType
Sweet orange leaf, field plant Acdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: CLK4_MOUSE (Dual specificity protein kinase CLK4 OS=Mus musculus GN=Clk4 PE=1 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 3.939e-24
Identity = 45/98 (45.92%), Postives = 64/98 (65.31%), Query Frame = 2 
Query:  380 DDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEKKEL-VAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICIL 667
            DD++GH +   G+ L  RY I+  +GEG FG++VEC D+    L VA+KIV+++ +YREAA  EI VL+ L   D     RCVQ+  WFD+  H+CI+
Sbjct:  141 DDEEGHLICQSGDVLRARYEIVDTLGEGAFGKVVECIDHGMDGLHVAVKIVKNVGRYREAARSEIQVLEHLNSTDPNSVFRCVQMLEWFDHHGHVCIV 238

HSP 2 Score: 39.2762 bits (90), Expect = 3.939e-24
Identity = 16/25 (64.00%), Postives = 20/25 (80.00%), Query Frame = 1 
Query:  664 FMHELRLIHTDLKPENILLVSAEYV 738
            F+H  +L HTDLKPENIL V ++YV
Sbjct:  276 FLHHNKLTHTDLKPENILFVKSDYV 300
[Close]
BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: CLK4_HUMAN (Dual specificity protein kinase CLK4 OS=Homo sapiens GN=CLK4 PE=1 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 6.670e-24
Identity = 44/98 (44.90%), Postives = 64/98 (65.31%), Query Frame = 2 
Query:  380 DDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEKKEL-VAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICIL 667
            DD++GH +   G+ L  RY I+  +GEG FG++VEC D+    + VA+KIV+++ +YREAA  EI VL+ L   D     RCVQ+  WFD+  H+CI+
Sbjct:  141 DDEEGHLICQSGDVLRARYEIVDTLGEGAFGKVVECIDHGMDGMHVAVKIVKNVGRYREAARSEIQVLEHLNSTDPNSVFRCVQMLEWFDHHGHVCIV 238

HSP 2 Score: 39.2762 bits (90), Expect = 6.670e-24
Identity = 16/25 (64.00%), Postives = 20/25 (80.00%), Query Frame = 1 
Query:  664 FMHELRLIHTDLKPENILLVSAEYV 738
            F+H  +L HTDLKPENIL V ++YV
Sbjct:  276 FLHHNKLTHTDLKPENILFVKSDYV 300
[Close]
BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: CLK2_MOUSE (Dual specificity protein kinase CLK2 OS=Mus musculus GN=Clk2 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 8.674e-24
Identity = 41/97 (42.27%), Postives = 67/97 (69.07%), Query Frame = 2 
Query:  380 DDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTR-CVQIRNWFDYRNHICI 664
            DD +GH ++ +G+ L  RY I+  +GEGT G++V+C D+ +    VA+KI++++ KY+EAA +EI+VL+++   D      CVQ+ +WFDY  H+CI
Sbjct:  144 DDAEGHLIYHVGDWLQERYEIVSTLGEGTSGRVVQCVDHRRGGTRVALKIIKNVEKYKEAARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCI 240

HSP 2 Score: 38.891 bits (89), Expect = 8.674e-24
Identity = 15/24 (62.50%), Postives = 21/24 (87.50%), Query Frame = 1 
Query:  664 FMHELRLIHTDLKPENILLVSAEY 735
            F+H+ +L HTDLKPENIL V+++Y
Sbjct:  279 FLHDNKLTHTDLKPENILFVNSDY 302
[Close]
BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: CLK1_MOUSE (Dual specificity protein kinase CLK1 OS=Mus musculus GN=Clk1 PE=1 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 1.469e-23
Identity = 45/98 (45.92%), Postives = 67/98 (68.37%), Query Frame = 2 
Query:  380 DDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNE-KKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICIL 667
            DD++GH +   G+ L+ RY I+  +GEG FG++VEC D++     VA+KIV+++++Y EAA  EI VL+ L   D   T RCVQ+  WF++R HICI+
Sbjct:  142 DDEEGHLICQSGDVLSARYEIVDTLGEGAFGKVVECIDHKVGGRRVAVKIVKNVDRYCEAAQSEIQVLEHLNTTDPHSTFRCVQMLEWFEHRGHICIV 239

HSP 2 Score: 38.1206 bits (87), Expect = 1.469e-23
Identity = 15/26 (57.69%), Postives = 20/26 (76.92%), Query Frame = 1 
Query:  664 FMHELRLIHTDLKPENILLVSAEYVK 741
            F+H  +L HTDLKPENIL V ++Y +
Sbjct:  277 FLHSNKLTHTDLKPENILFVKSDYTE 302
[Close]
BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: CLK1_HUMAN (Dual specificity protein kinase CLK1 OS=Homo sapiens GN=CLK1 PE=1 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 3.237e-23
Identity = 44/98 (44.90%), Postives = 66/98 (67.35%), Query Frame = 2 
Query:  380 DDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICIL 667
            DD++GH +   G+ L+ RY I+  +GEG FG++VEC D++     VA+KIV+++++Y EAA  EI VL+ L   D   T RCVQ+  WF++  HICI+
Sbjct:  143 DDEEGHLICQSGDVLSARYEIVDTLGEGAFGKVVECIDHKAGGRHVAVKIVKNVDRYCEAARSEIQVLEHLNTTDPNSTFRCVQMLEWFEHHGHICIV 240

HSP 2 Score: 38.5058 bits (88), Expect = 3.237e-23
Identity = 16/39 (41.03%), Postives = 24/39 (61.54%), Query Frame = 1 
Query:  664 FMHELRLIHTDLKPENILLVSAEYVKVPDYSFYHDRQRM 780
            F+H  +L HTDLKPENIL V ++Y +  +     D + +
Sbjct:  278 FLHSNKLTHTDLKPENILFVQSDYTEAYNPKIKRDERTL 316
[Close]
BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: CLKA_DICDI (Probable serine/threonine-protein kinase clkA OS=Dictyostelium discoideum GN=clkA PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 7.052e-23
Identity = 43/117 (36.75%), Postives = 69/117 (58.97%), Query Frame = 2 
Query:  347 IPRTGSPPWRPDDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGG----TRCVQIRNWFDYRNHICILCMNYVL 685
            I ++    W  DD++ +Y   +GE L  RY++L  +G GTF  +VEC+D      VAIKIVRS  KY E A++EID+L+ L +         + C+++ + F +++HIC++   Y L
Sbjct:  563 IEKSNKSMW--DDENDYYKVQVGEYLNNRYKVLCTVGSGTFSTVVECWDTNSSGQVAIKIVRSAKKYTEDALVEIDILRNLEKTGNSNGKYLSHCIRLLDSFLFKDHICLVFKRYGL 677

HSP 2 Score: 41.2022 bits (95), Expect = 7.052e-23
Identity = 17/26 (65.38%), Postives = 22/26 (84.62%), Query Frame = 1 
Query:  658 LYFMHELRLIHTDLKPENILLVSAEY 735
            +Y MH+L L+HTDLKPENILL S+ +
Sbjct:  707 IYSMHKLSLVHTDLKPENILLESSRF 732
[Close]
BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: KNS1_YEAST (Dual specificity protein kinase KNS1 OS=Saccharomyces cerevisiae GN=KNS1 PE=1 SV=2)

HSP 1 Score: 88.5817 bits (218), Expect = 2.028e-22
Identity = 41/99 (41.41%), Postives = 69/99 (69.70%), Query Frame = 2 
Query:  383 DKDGHYVFAIGENLTPRYRILIK--MGEGTFGQLVECFDNE-KKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICIL 667
            DKDGHYV+   +      R ++K  +G+GTFG++++C DN+ +   VA+K++R++++YREAA  E+ +LQ +  ND  G  +C+ +R  FDY+NHIC++
Sbjct:  294 DKDGHYVYQENDIFGSGGRFVVKDLLGQGTFGKVLKCIDNKYEPNYVAVKVIRAVDRYREAAKTELRILQTILNNDPQGQFQCLLLRECFDYKNHICLV 392

HSP 2 Score: 36.965 bits (84), Expect = 2.028e-22
Identity = 14/25 (56.00%), Postives = 20/25 (80.00%), Query Frame = 1 
Query:  664 FMHELRLIHTDLKPENILLVSAEYV 738
            F+H+L +IHTDLKPENIL+    ++
Sbjct:  430 FLHDLGIIHTDLKPENILICDETHI 454
[Close]
BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: DYRK4_MOUSE (Dual specificity tyrosine-phosphorylation-regulated kinase 4 OS=Mus musculus GN=Dyrk4 PE=1 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 7.481e-18
Identity = 34/96 (35.42%), Postives = 64/96 (66.67%), Query Frame = 2 
Query:  380 DDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 664
            DD+ G Y+  + +++  RY +L  +G+G+FGQ+ +C D++  ELVA+KI+R+  ++   A++E+ +L+ L R D       V ++++F +RNH+CI
Sbjct:  201 DDEHGSYMKVLHDHIAYRYEVLEMIGKGSFGQVAKCLDHKNNELVALKIIRNKKRFHHQALVELKILEALRRKDKDNNHNVVHMKDFFYFRNHLCI 296

HSP 2 Score: 29.6462 bits (65), Expect = 7.481e-18
Identity = 14/34 (41.18%), Postives = 20/34 (58.82%), Query Frame = 1 
Query:  658 LYFMHELRLIHTDLKPENILLVSAEYVKVPDYSF 759
            L+ ++  ++IH DLKPENI+L     V V    F
Sbjct:  333 LHMLYVEKIIHCDLKPENIVLYQRGQVTVKVIDF 366
[Close]
BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: MBK2_CAEEL (Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 OS=Caenorhabditis elegans GN=mbk-2 PE=1 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.333e-16
Identity = 34/96 (35.42%), Postives = 63/96 (65.62%), Query Frame = 2 
Query:  380 DDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 664
            DD++G Y   + +++  RY +L  +G+G+FGQ+++ FD++ ++ VA+K+VR+  ++   A  EI +L  L R D  GT   + + ++F++RNH CI
Sbjct:  443 DDENGSYQLVVHDHIAYRYEVLKVIGKGSFGQVIKAFDHKYQQYVALKLVRNEKRFHRQADEEIRILDHLRRQDSDGTHNIIHMLDYFNFRNHKCI 538

HSP 2 Score: 30.0314 bits (66), Expect = 1.333e-16
Identity = 20/44 (45.45%), Postives = 26/44 (59.09%), Query Frame = 1 
Query:  658 LYFMHELRLIHTDLKPENILL--VSAEYVKVPDY-SFYHDRQRM 780
            L  + + RLIH DLKPEN+LL       +KV D+ S   D QR+
Sbjct:  575 LDLLQKNRLIHCDLKPENVLLKQQGRSGIKVIDFGSSCFDDQRI 618
[Close]
BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: MBK2_CAEBR (Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 OS=Caenorhabditis briggsae GN=mbk-2 PE=3 SV=2)

HSP 1 Score: 75.8702 bits (185), Expect = 1.333e-16
Identity = 34/96 (35.42%), Postives = 63/96 (65.62%), Query Frame = 2 
Query:  380 DDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 664
            DD++G Y   + +++  RY +L  +G+G+FGQ+++ FD++ ++ VA+K+VR+  ++   A  EI +L  L R D  GT   + + ++F++RNH CI
Sbjct:  442 DDENGSYQLVVHDHIAYRYEVLKVIGKGSFGQVIKAFDHKYQQYVALKLVRNEKRFHRQADEEIRILDHLRRQDSDGTHNIIHMLDYFNFRNHKCI 537

HSP 2 Score: 30.0314 bits (66), Expect = 1.333e-16
Identity = 20/44 (45.45%), Postives = 26/44 (59.09%), Query Frame = 1 
Query:  658 LYFMHELRLIHTDLKPENILL--VSAEYVKVPDY-SFYHDRQRM 780
            L  + + RLIH DLKPEN+LL       +KV D+ S   D QR+
Sbjct:  574 LDLLQKNRLIHCDLKPENVLLKQQGRSGIKVIDFGSSCFDDQRI 617
The following BLAST results are available for this feature:
BLAST of EY754217 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 48
Match NameE-valueIdentityDescription
CLK4_MOUSE3.939e-2445.92Dual specificity protein kinase CLK4 OS=Mus muscul... [more]
CLK4_HUMAN6.670e-2444.90Dual specificity protein kinase CLK4 OS=Homo sapie... [more]
CLK2_MOUSE8.674e-2442.27Dual specificity protein kinase CLK2 OS=Mus muscul... [more]
CLK1_MOUSE1.469e-2345.92Dual specificity protein kinase CLK1 OS=Mus muscul... [more]
CLK1_HUMAN3.237e-2344.90Dual specificity protein kinase CLK1 OS=Homo sapie... [more]
CLKA_DICDI7.052e-2336.75Probable serine/threonine-protein kinase clkA OS=D... [more]
KNS1_YEAST2.028e-2241.41Dual specificity protein kinase KNS1 OS=Saccharomy... [more]
DYRK4_MOUSE7.481e-1835.42Dual specificity tyrosine-phosphorylation-regulate... [more]
MBK2_CAEEL1.333e-1635.42Dual specificity tyrosine-phosphorylation-regulate... [more]
MBK2_CAEBR1.333e-1635.42Dual specificity tyrosine-phosphorylation-regulate... [more]

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Properties
Property NameValue
Genbank descriptionCS12-C1-001-012-B02-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY754217 ID=EY754217; Name=EY754217; organism=Citrus sinensis; type=EST; length=861bp
AATCCCCATTTAGGGTTTCCAACACTGAAAAACATTTCTTCAAAATTTCC
TTAATTTTTTACTCTCAATTTTTTTTAAAGCATTATGATTCAAATTGAGC
TTAATCCATGGCTCTGCTAGGTTTTTTTAATTGAAAATTTAAACAATAAA
AGAAATGGAAACGCACAGAATTATAGAATTTCCGCATAAAAACATGGACA
AGCGTCCCAAAAAACGACCCCGTTTAACGTGGGACGTTCCGCCTCCTCTT
CCTCCTCCCAAGGTTCTTCCGGCTCTGTATTGTGTTCAGGAGTTTGGGAA
TGGAGGAATGCCCAATTATGCCTGTTCATCCATGTTTTATGGTGCGATTC
CTCGCACCGGATCACCTCCTTGGAGACCTGATGATAAAGACGGACATTAC
GTTTTTGCCATCGGAGAAAATTTAACTCCTCGCTACAGGATTCTCATCAA
AATGGGTGAAGGGACATTTGGGCAACTCGTGGAATGTTTTGACAATGAGA
AGAAGGAACTTGTGGCAATCAAAATTGTTCGCTCTATAAACAAGTATCGT
GAAGCAGCCATGATCGAAATTGATGTTCTACAGAGGCTTGCCAGGAATGA
CATTGGTGGCACCCGTTGTGTGCAAATACGGAATTGGTTTGACTATCGTA
ATCATATCTGTATTTTATGCATGAATTACGTCTTATACACACTGACTTGA
AGCCAGAAAATATTCTTCTTGTTTCCGCTGAGTATGTTAAAGTCCCTGAT
TATAGTTTCTATCACGATCGTCAAAGGATGGGTTCTATTTCAGGATCTTC
CCAGTGCAGTGCATTAAGCTAATTGATTTTGGAGCACACATTTGACCTCA
GGATCCAGCTT
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