EY754176

Overview
NameEY754176
Unique NameEY754176
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length921
Libraries
Library NameType
Sweet orange leaf, field plant Acdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: CLK4_MOUSE (Dual specificity protein kinase CLK4 OS=Mus musculus GN=Clk4 PE=1 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.467e-24
Identity = 46/98 (46.94%), Postives = 64/98 (65.31%), Query Frame = 3 
Query:  411 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEKKEL-VAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICIL 698
            DD++GH +   G+ L  RY I+  +GEG FG+VVEC D+    L VA+KIV+++ +YREAA  EI VL+ L   D     RCVQ+  WFD+  H+CI+
Sbjct:  141 DDEEGHLICQSGDVLRARYEIVDTLGEGAFGKVVECIDHGMDGLHVAVKIVKNVGRYREAARSEIQVLEHLNSTDPNSVFRCVQMLEWFDHHGHVCIV 238

HSP 2 Score: 40.0466 bits (92), Expect = 1.467e-24
Identity = 20/45 (44.44%), Postives = 27/45 (60.00%), Query Frame = 2 
Query:  695 FMHELRLIHTDLKPXNILLVSAEYVKVPDYKFLSRSSKDGSYFKN 829
            F+H  +L HTDLKP NIL V ++YV     K+ S+  +D    KN
Sbjct:  276 FLHHNKLTHTDLKPENILFVKSDYV----VKYNSKMKRDERTLKN 316
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: CLK4_HUMAN (Dual specificity protein kinase CLK4 OS=Homo sapiens GN=CLK4 PE=1 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 2.485e-24
Identity = 45/98 (45.92%), Postives = 64/98 (65.31%), Query Frame = 3 
Query:  411 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEKKEL-VAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICIL 698
            DD++GH +   G+ L  RY I+  +GEG FG+VVEC D+    + VA+KIV+++ +YREAA  EI VL+ L   D     RCVQ+  WFD+  H+CI+
Sbjct:  141 DDEEGHLICQSGDVLRARYEIVDTLGEGAFGKVVECIDHGMDGMHVAVKIVKNVGRYREAARSEIQVLEHLNSTDPNSVFRCVQMLEWFDHHGHVCIV 238

HSP 2 Score: 40.0466 bits (92), Expect = 2.485e-24
Identity = 20/45 (44.44%), Postives = 27/45 (60.00%), Query Frame = 2 
Query:  695 FMHELRLIHTDLKPXNILLVSAEYVKVPDYKFLSRSSKDGSYFKN 829
            F+H  +L HTDLKP NIL V ++YV     K+ S+  +D    KN
Sbjct:  276 FLHHNKLTHTDLKPENILFVKSDYV----VKYNSKMKRDERTLKN 316
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: CLK2_MOUSE (Dual specificity protein kinase CLK2 OS=Mus musculus GN=Clk2 PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.206e-23
Identity = 43/97 (44.33%), Postives = 68/97 (70.10%), Query Frame = 3 
Query:  411 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTR-CVQIRNWFDYRNHICI 695
            DD +GH ++ +G+ L  RY I+S +GEGT G+VV+C D+ +    VA+KI++++ KY+EAA +EI+VL+++   D      CVQ+ +WFDY  H+CI
Sbjct:  144 DDAEGHLIYHVGDWLQERYEIVSTLGEGTSGRVVQCVDHRRGGTRVALKIIKNVEKYKEAARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCI 240

HSP 2 Score: 36.5798 bits (83), Expect = 1.206e-23
Identity = 14/24 (58.33%), Postives = 20/24 (83.33%), Query Frame = 2 
Query:  695 FMHELRLIHTDLKPXNILLVSAEY 766
            F+H+ +L HTDLKP NIL V+++Y
Sbjct:  279 FLHDNKLTHTDLKPENILFVNSDY 302
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: CLK1_MOUSE (Dual specificity protein kinase CLK1 OS=Mus musculus GN=Clk1 PE=1 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 4.501e-23
Identity = 46/98 (46.94%), Postives = 67/98 (68.37%), Query Frame = 3 
Query:  411 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNE-KKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICIL 698
            DD++GH +   G+ L+ RY I+  +GEG FG+VVEC D++     VA+KIV+++++Y EAA  EI VL+ L   D   T RCVQ+  WF++R HICI+
Sbjct:  142 DDEEGHLICQSGDVLSARYEIVDTLGEGAFGKVVECIDHKVGGRRVAVKIVKNVDRYCEAAQSEIQVLEHLNTTDPHSTFRCVQMLEWFEHRGHICIV 239

HSP 2 Score: 35.8094 bits (81), Expect = 4.501e-23
Identity = 14/26 (53.85%), Postives = 19/26 (73.08%), Query Frame = 2 
Query:  695 FMHELRLIHTDLKPXNILLVSAEYVK 772
            F+H  +L HTDLKP NIL V ++Y +
Sbjct:  277 FLHSNKLTHTDLKPENILFVKSDYTE 302
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: CLK1_HUMAN (Dual specificity protein kinase CLK1 OS=Homo sapiens GN=CLK1 PE=1 SV=2)

HSP 1 Score: 92.0485 bits (227), Expect = 1.290e-22
Identity = 45/98 (45.92%), Postives = 66/98 (67.35%), Query Frame = 3 
Query:  411 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICIL 698
            DD++GH +   G+ L+ RY I+  +GEG FG+VVEC D++     VA+KIV+++++Y EAA  EI VL+ L   D   T RCVQ+  WF++  HICI+
Sbjct:  143 DDEEGHLICQSGDVLSARYEIVDTLGEGAFGKVVECIDHKAGGRHVAVKIVKNVDRYCEAARSEIQVLEHLNTTDPNSTFRCVQMLEWFEHHGHICIV 240

HSP 2 Score: 35.8094 bits (81), Expect = 1.290e-22
Identity = 14/26 (53.85%), Postives = 19/26 (73.08%), Query Frame = 2 
Query:  695 FMHELRLIHTDLKPXNILLVSAEYVK 772
            F+H  +L HTDLKP NIL V ++Y +
Sbjct:  278 FLHSNKLTHTDLKPENILFVQSDYTE 303
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: CLKA_DICDI (Probable serine/threonine-protein kinase clkA OS=Dictyostelium discoideum GN=clkA PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 4.752e-22
Identity = 44/117 (37.61%), Postives = 69/117 (58.97%), Query Frame = 3 
Query:  378 IPRTGSPPWRPDDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGG----TRCVQIRNWFDYRNHICILCMNYVL 716
            I ++    W  DD++ +Y   +GE L  RY++L  +G GTF  VVEC+D      VAIKIVRS  KY E A++EID+L+ L +         + C+++ + F +++HIC++   Y L
Sbjct:  563 IEKSNKSMW--DDENDYYKVQVGEYLNNRYKVLCTVGSGTFSTVVECWDTNSSGQVAIKIVRSAKKYTEDALVEIDILRNLEKTGNSNGKYLSHCIRLLDSFLFKDHICLVFKRYGL 677

HSP 2 Score: 38.891 bits (89), Expect = 4.752e-22
Identity = 16/26 (61.54%), Postives = 21/26 (80.77%), Query Frame = 2 
Query:  689 LYFMHELRLIHTDLKPXNILLVSAEY 766
            +Y MH+L L+HTDLKP NILL S+ +
Sbjct:  707 IYSMHKLSLVHTDLKPENILLESSRF 732
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: KNS1_YEAST (Dual specificity protein kinase KNS1 OS=Saccharomyces cerevisiae GN=KNS1 PE=1 SV=2)

HSP 1 Score: 87.8113 bits (216), Expect = 5.090e-21
Identity = 42/99 (42.42%), Postives = 68/99 (68.69%), Query Frame = 3 
Query:  414 DKDGHYVFAIGENLTPRYRILSK--MGEGTFGQVVECFDNE-KKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICIL 698
            DKDGHYV+   +      R + K  +G+GTFG+V++C DN+ +   VA+K++R++++YREAA  E+ +LQ +  ND  G  +C+ +R  FDY+NHIC++
Sbjct:  294 DKDGHYVYQENDIFGSGGRFVVKDLLGQGTFGKVLKCIDNKYEPNYVAVKVIRAVDRYREAAKTELRILQTILNNDPQGQFQCLLLRECFDYKNHICLV 392

HSP 2 Score: 34.6538 bits (78), Expect = 5.090e-21
Identity = 13/25 (52.00%), Postives = 19/25 (76.00%), Query Frame = 2 
Query:  695 FMHELRLIHTDLKPXNILLVSAEYV 769
            F+H+L +IHTDLKP NIL+    ++
Sbjct:  430 FLHDLGIIHTDLKPENILICDETHI 454
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: DYRK4_MOUSE (Dual specificity tyrosine-phosphorylation-regulated kinase 4 OS=Mus musculus GN=Dyrk4 PE=1 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 2.967e-17
Identity = 35/96 (36.46%), Postives = 64/96 (66.67%), Query Frame = 3 
Query:  411 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 695
            DD+ G Y+  + +++  RY +L  +G+G+FGQV +C D++  ELVA+KI+R+  ++   A++E+ +L+ L R D       V ++++F +RNH+CI
Sbjct:  201 DDEHGSYMKVLHDHIAYRYEVLEMIGKGSFGQVAKCLDHKNNELVALKIIRNKKRFHHQALVELKILEALRRKDKDNNHNVVHMKDFFYFRNHLCI 296

HSP 2 Score: 26.9498 bits (58), Expect = 2.967e-17
Identity = 12/29 (41.38%), Postives = 18/29 (62.07%), Query Frame = 2 
Query:  689 LYFMHELRLIHTDLKPXNILLVSAEYVKV 775
            L+ ++  ++IH DLKP NI+L     V V
Sbjct:  333 LHMLYVEKIIHCDLKPENIVLYQRGQVTV 361
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: MBK2_CAEEL (Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 OS=Caenorhabditis elegans GN=mbk-2 PE=1 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 5.279e-16
Identity = 35/96 (36.46%), Postives = 63/96 (65.62%), Query Frame = 3 
Query:  411 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 695
            DD++G Y   + +++  RY +L  +G+G+FGQV++ FD++ ++ VA+K+VR+  ++   A  EI +L  L R D  GT   + + ++F++RNH CI
Sbjct:  443 DDENGSYQLVVHDHIAYRYEVLKVIGKGSFGQVIKAFDHKYQQYVALKLVRNEKRFHRQADEEIRILDHLRRQDSDGTHNIIHMLDYFNFRNHKCI 538

HSP 2 Score: 27.335 bits (59), Expect = 5.279e-16
Identity = 12/21 (57.14%), Postives = 15/21 (71.43%), Query Frame = 2 
Query:  689 LYFMHELRLIHTDLKPXNILL 751
            L  + + RLIH DLKP N+LL
Sbjct:  575 LDLLQKNRLIHCDLKPENVLL 595
[Close]
BLAST of EY754176 vs. ExPASy Swiss-Prot
Match: MBK2_CAEBR (Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 OS=Caenorhabditis briggsae GN=mbk-2 PE=3 SV=2)

HSP 1 Score: 78.1814 bits (191), Expect = 5.279e-16
Identity = 35/96 (36.46%), Postives = 63/96 (65.62%), Query Frame = 3 
Query:  411 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 695
            DD++G Y   + +++  RY +L  +G+G+FGQV++ FD++ ++ VA+K+VR+  ++   A  EI +L  L R D  GT   + + ++F++RNH CI
Sbjct:  442 DDENGSYQLVVHDHIAYRYEVLKVIGKGSFGQVIKAFDHKYQQYVALKLVRNEKRFHRQADEEIRILDHLRRQDSDGTHNIIHMLDYFNFRNHKCI 537

HSP 2 Score: 27.335 bits (59), Expect = 5.279e-16
Identity = 12/21 (57.14%), Postives = 15/21 (71.43%), Query Frame = 2 
Query:  689 LYFMHELRLIHTDLKPXNILL 751
            L  + + RLIH DLKP N+LL
Sbjct:  574 LDLLQKNRLIHCDLKPENVLL 594
The following BLAST results are available for this feature:
BLAST of EY754176 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 53
Match NameE-valueIdentityDescription
CLK4_MOUSE1.467e-2446.94Dual specificity protein kinase CLK4 OS=Mus muscul... [more]
CLK4_HUMAN2.485e-2445.92Dual specificity protein kinase CLK4 OS=Homo sapie... [more]
CLK2_MOUSE1.206e-2344.33Dual specificity protein kinase CLK2 OS=Mus muscul... [more]
CLK1_MOUSE4.501e-2346.94Dual specificity protein kinase CLK1 OS=Mus muscul... [more]
CLK1_HUMAN1.290e-2245.92Dual specificity protein kinase CLK1 OS=Homo sapie... [more]
CLKA_DICDI4.752e-2237.61Probable serine/threonine-protein kinase clkA OS=D... [more]
KNS1_YEAST5.090e-2142.42Dual specificity protein kinase KNS1 OS=Saccharomy... [more]
DYRK4_MOUSE2.967e-1736.46Dual specificity tyrosine-phosphorylation-regulate... [more]
MBK2_CAEEL5.279e-1636.46Dual specificity tyrosine-phosphorylation-regulate... [more]
MBK2_CAEBR5.279e-1636.46Dual specificity tyrosine-phosphorylation-regulate... [more]

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Properties
Property NameValue
Genbank descriptionCS12-C1-001-011-F03-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY754176 ID=EY754176; Name=EY754176; organism=Citrus sinensis; type=EST; length=921bp
CCGGNATTCCACGCTCGACCCACGCGTCCGAATCCCCTTTTAGGGTTTCC
AACACTNGAAAATCATCTCTTCAAAATTTCCTTAATTTTTTACTCTCAAT
TTTTTTTAAAGCATTATGATTCAAATTGAGCTTAATCCATGGCTCTGCTA
GGTTTTTTTAATTGAAAATTTAAACAATAAAAGAAATGGAAACGCAGAGA
ATTATAGAATTTCCGCATAAAAACATGGACAAGCGTCCCAGAAAACGACC
CCGTTTAACGTGGGACGTTCCGCCTCCTCTTCCTCCTCCCAAGGTTCTTC
CGGCTCTGTATTGTGTTCAGGAGTTTGGGAATGGAGGAATGCCCAATTAT
GCCTGTTCATCCATGTTTTATGGTGCGATTCCTCGCACCGGATCACCTCC
TTGGAGACCTGATGATAAAGACGGACATTACGTTTTTGCCATCGGAGAAA
ATTTAACTCCTCGCTACAGGATTCTCAGCAAAATGGGTGAAGGGACATTT
GGGCAAGTCGTGGAATGTTTTGACAATGAGAAGAAGGAACTTGTGGCAAT
CAAAATTGTTCGCTCTATAAACAAGTATCGTGAAGCAGCCATGATCGAAA
TTGATGTTCTACAGAGGCTTGCCAGGAATGACATTGGTGGCACCCGTTGT
GTGCAAATACGGAATTGGTTTGACTATCGTAATCATATTTGTATTTTATG
CATGAATTACGTCTTATACACACTGACTTGAAGCCANGAAATATTCTTCT
TGTTTCCGCTGAGTATGTTAAAGTCCCTGATTATAAGTTTCTATCACGAT
CGTCAAAAGATGGTTCCTATTTCAAGAATCTCCCCAAGTCAGTGCATTTA
GCTAATTGATTTGGGAACACACATTTGAACATCAGATCCAGCTTTTGTGG
TTAACACCACTTATCTGCCCC
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