GE213243

Overview
NameGE213243
Unique NameGE213243
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length394
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB_PORPU (Tubulin beta chain OS=Porphyra purpurea GN=TUBB1 PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 4.173e-28
Identity = 36/58 (62.07%), Postives = 43/58 (74.14%), Query Frame = 3 
Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVK 230
            M+D KNMM   DPR GRYLT SA FRG +STKEV+EQM  ++ K S  F+DWIPN +K
Sbjct:  293 MFDPKNMMADCDPRNGRYLTASAYFRGHVSTKEVEEQMDAIQTKNSGQFIDWIPNAIK 350

HSP 2 Score: 68.9366 bits (167), Expect = 4.173e-28
Identity = 29/49 (59.18%), Postives = 37/49 (75.51%), Query Frame = 2 
Query:  227 EVSVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            + SVCD+ P G +M + F GNST+IQ +F+RV   F AMF+RKAFLHWY
Sbjct:  350 KASVCDVAPTGETMSAAFIGNSTAIQDIFKRVGSHFSAMFKRKAFLHWY 398
[Close]
BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB1_CHOCR (Tubulin beta-1 chain OS=Chondrus crispus GN=TUBB PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 7.592e-27
Identity = 33/58 (56.90%), Postives = 45/58 (77.59%), Query Frame = 3 
Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVK 230
            M+D  NMM   DPR GRYLT +  FRGK+STKEV+++M  ++ K S+YFV+WIP+N+K
Sbjct:  295 MFDRNNMMAEIDPREGRYLTAAVYFRGKVSTKEVEDEMTLMQTKNSAYFVEWIPHNIK 352

HSP 2 Score: 66.2402 bits (160), Expect = 7.592e-27
Identity = 28/49 (57.14%), Postives = 36/49 (73.47%), Query Frame = 2 
Query:  227 EVSVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            + SVCDIP  G  + S F GN+T+I+  F+R   QF++MFRRKAFLHWY
Sbjct:  352 KTSVCDIPAAGEKISSAFIGNTTAIEATFKRFGNQFRSMFRRKAFLHWY 400
[Close]
BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB2_GEOCN (Tubulin beta-2 chain OS=Geotrichum candidum PE=3 SV=2)

HSP 1 Score: 69.3218 bits (168), Expect = 5.151e-25
Identity = 26/49 (53.06%), Postives = 38/49 (77.55%), Query Frame = 2 
Query:  227 EVSVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            + SVC++PP+GL+  +TF  NST++Q++F R + QF  MF+RK FLHWY
Sbjct:  352 QTSVCNVPPKGLTTSATFIANSTAVQELFERTANQFSVMFKRKGFLHWY 400

HSP 2 Score: 66.6254 bits (161), Expect = 5.151e-25
Identity = 28/58 (48.28%), Postives = 43/58 (74.14%), Query Frame = 3 
Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVK 230
            +++  N+M A +P  GRYLT+S +FRG+++ KEV++ +   R K S YFV+WIPNNV+
Sbjct:  295 LFNPANVMAACNPYHGRYLTISTIFRGQVAMKEVEDAIHTARTKYSPYFVEWIPNNVQ 352
[Close]
BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB8_ARATH (Tubulin beta-8 chain OS=Arabidopsis thaliana GN=TUBB8 PE=2 SV=2)

HSP 1 Score: 110.538 bits (275), Expect = 2.640e-24
Identity = 59/92 (64.13%), Postives = 66/92 (71.74%), Query Frame = 3 
Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVKLVFV----TFLPEVSPWYPPSSGTQHPSKKCSEE 320
            MWDAKNMM AADPR GRYLT SAMFRGKMSTKEVDEQMINV+NK SSYFV+WIPNNVK        T L   S +   S+  Q   ++ SE+
Sbjct:  293 MWDAKNMMCAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSTVCDIPPTGLKMASTFIGNSTSIQEMFRRVSEQ 384

HSP 2 Score: 87.8113 bits (216), Expect = 1.826e-17
Identity = 40/47 (85.11%), Postives = 42/47 (89.36%), Query Frame = 2 
Query:  233 SVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            +VCDIPP GL M STF GNSTSIQ+MFRRVSEQF AMFRRKAFLHWY
Sbjct:  352 TVCDIPPTGLKMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWY 398
[Close]
BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB4_MAIZE (Tubulin beta-4 chain OS=Zea mays GN=TUBB4 PE=2 SV=2)

HSP 1 Score: 110.538 bits (275), Expect = 2.640e-24
Identity = 58/92 (63.04%), Postives = 66/92 (71.74%), Query Frame = 3 
Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVKLVFVTF----LPEVSPWYPPSSGTQHPSKKCSEE 320
            MWDAKNMM +ADPR GRYLT SAMFRGKMSTKEVDEQMINV+NK SSYFV+WIPNNVK          LP  S +   S+  Q   ++ SE+
Sbjct:  295 MWDAKNMMCSADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPVGLPMASTFVGNSTSIQEMFRRVSEQ 386

HSP 2 Score: 87.4261 bits (215), Expect = 2.384e-17
Identity = 41/47 (87.23%), Postives = 42/47 (89.36%), Query Frame = 2 
Query:  233 SVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            SVCDIPP GL M STF GNSTSIQ+MFRRVSEQF AMFRRKAFLHWY
Sbjct:  354 SVCDIPPVGLPMASTFVGNSTSIQEMFRRVSEQFTAMFRRKAFLHWY 400
[Close]
BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB2_LUPAL (Tubulin beta-2 chain OS=Lupinus albus GN=TUBB2 PE=3 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 2.640e-24
Identity = 59/92 (64.13%), Postives = 66/92 (71.74%), Query Frame = 3 
Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVKLVFV----TFLPEVSPWYPPSSGTQHPSKKCSEE 320
            MWDAKNMM AADPR GRYLT SAMFRGKMSTKEVDEQMINV+NK SSYFV+WIPNNVK        T L   S +   S+  Q   ++ SE+
Sbjct:  293 MWDAKNMMCAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSTVCDIPPTGLKMASTFIGNSTSIQEMFRRVSEQ 384

HSP 2 Score: 87.8113 bits (216), Expect = 1.826e-17
Identity = 40/47 (85.11%), Postives = 42/47 (89.36%), Query Frame = 2 
Query:  233 SVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            +VCDIPP GL M STF GNSTSIQ+MFRRVSEQF AMFRRKAFLHWY
Sbjct:  352 TVCDIPPTGLKMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWY 398
[Close]
BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB1_LUPAL (Tubulin beta-1 chain OS=Lupinus albus GN=TUBB1 PE=3 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 2.640e-24
Identity = 59/92 (64.13%), Postives = 66/92 (71.74%), Query Frame = 3 
Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVKLVFV----TFLPEVSPWYPPSSGTQHPSKKCSEE 320
            MWDAKNMM AADPR GRYLT SAMFRGKMSTKEVDEQMINV+NK SSYFV+WIPNNVK        T L   S +   S+  Q   ++ SE+
Sbjct:  293 MWDAKNMMCAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSTVCDIPPTGLKMASTFIGNSTSIQEMFRRVSEQ 384

HSP 2 Score: 87.8113 bits (216), Expect = 1.826e-17
Identity = 40/47 (85.11%), Postives = 42/47 (89.36%), Query Frame = 2 
Query:  233 SVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            +VCDIPP GL M STF GNSTSIQ+MFRRVSEQF AMFRRKAFLHWY
Sbjct:  352 TVCDIPPTGLKMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWY 398
[Close]
BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB_HORVU (Tubulin beta chain OS=Hordeum vulgare GN=TUBB PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 3.449e-24
Identity = 59/92 (64.13%), Postives = 67/92 (72.83%), Query Frame = 3 
Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVKL----VFVTFLPEVSPWYPPSSGTQHPSKKCSEE 320
            MWDAKNMM AADPR GRYLT SAMFRGKMSTKEVDEQMINV+NK SSYFV+WIPNNVK     +  T L   S +   S+  Q   ++ SE+
Sbjct:  293 MWDAKNMMCAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTGLSMASTFVGNSTSIQEMFRRVSEQ 384

HSP 2 Score: 90.1225 bits (222), Expect = 3.679e-18
Identity = 42/47 (89.36%), Postives = 43/47 (91.49%), Query Frame = 2 
Query:  233 SVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            SVCDIPP GLSM STF GNSTSIQ+MFRRVSEQF AMFRRKAFLHWY
Sbjct:  352 SVCDIPPTGLSMASTFVGNSTSIQEMFRRVSEQFTAMFRRKAFLHWY 398
[Close]
BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB3_SOYBN (Tubulin beta chain (Fragment) OS=Glycine max GN=TUBB PE=2 SV=2)

HSP 1 Score: 110.153 bits (274), Expect = 3.449e-24
Identity = 58/92 (63.04%), Postives = 66/92 (71.74%), Query Frame = 3 
Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVKLVFVTFLPE----VSPWYPPSSGTQHPSKKCSEE 320
            MWDAKNMM AADPR GRYLT SAMFRGKMSTKEVDEQMINV+NK SSYFV+WIPNNVK       P+     S +   S+  Q   ++ SE+
Sbjct:  293 MWDAKNMMCAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPKGLKMASTFIGNSTSIQEMFRRVSEQ 384

HSP 2 Score: 90.1225 bits (222), Expect = 3.679e-18
Identity = 41/47 (87.23%), Postives = 43/47 (91.49%), Query Frame = 2 
Query:  233 SVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            SVCDIPP+GL M STF GNSTSIQ+MFRRVSEQF AMFRRKAFLHWY
Sbjct:  352 SVCDIPPKGLKMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWY 398
[Close]
BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB2_WHEAT (Tubulin beta-2 chain OS=Triticum aestivum GN=TUBB2 PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 3.449e-24
Identity = 59/92 (64.13%), Postives = 67/92 (72.83%), Query Frame = 3 
Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVKL----VFVTFLPEVSPWYPPSSGTQHPSKKCSEE 320
            MWDAKNMM AADPR GRYLT SAMFRGKMSTKEVDEQMINV+NK SSYFV+WIPNNVK     +  T L   S +   S+  Q   ++ SE+
Sbjct:  293 MWDAKNMMCAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTGLSMASTFVGNSTSIQEMFRRVSEQ 384

HSP 2 Score: 88.9669 bits (219), Expect = 8.195e-18
Identity = 41/47 (87.23%), Postives = 43/47 (91.49%), Query Frame = 2 
Query:  233 SVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            SVCDIPP GLSM STF GNSTSIQ+MFRRVSEQF +MFRRKAFLHWY
Sbjct:  352 SVCDIPPTGLSMASTFVGNSTSIQEMFRRVSEQFTSMFRRKAFLHWY 398
The following BLAST results are available for this feature:
BLAST of GE213243 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 238
Match NameE-valueIdentityDescription
TBB_PORPU4.173e-2862.07Tubulin beta chain OS=Porphyra purpurea GN=TUBB1 P... [more]
TBB1_CHOCR7.592e-2756.90Tubulin beta-1 chain OS=Chondrus crispus GN=TUBB P... [more]
TBB2_GEOCN5.151e-2553.06Tubulin beta-2 chain OS=Geotrichum candidum PE=3 S... [more]
TBB8_ARATH2.640e-2464.13Tubulin beta-8 chain OS=Arabidopsis thaliana GN=TU... [more]
TBB4_MAIZE2.640e-2463.04Tubulin beta-4 chain OS=Zea mays GN=TUBB4 PE=2 SV=... [more]
TBB2_LUPAL2.640e-2464.13Tubulin beta-2 chain OS=Lupinus albus GN=TUBB2 PE=... [more]
TBB1_LUPAL2.640e-2464.13Tubulin beta-1 chain OS=Lupinus albus GN=TUBB1 PE=... [more]
TBB_HORVU3.449e-2464.13Tubulin beta chain OS=Hordeum vulgare GN=TUBB PE=2... [more]
TBB3_SOYBN3.449e-2463.04Tubulin beta chain (Fragment) OS=Glycine max GN=TU... [more]
TBB2_WHEAT3.449e-2464.13Tubulin beta-2 chain OS=Triticum aestivum GN=TUBB2... [more]

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Properties
Property NameValue
Genbank description92 BH (somaclonal variated plants of Skaggs Bonanza navel orange) SSH library Citrus sinensis cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>GE213243 ID=GE213243; Name=GE213243; organism=Citrus sinensis; type=EST; length=394bp
ATTACCAGGGTCCCGGCCGAGGTACCGTGCCTTGATTTTTTTTTTTTTTT
TTTTTTATGTGGGATGCCAAGAACATGATGGGTGCTGCCGACCCAAGGTT
TGGTCGTTACCTCACTGTTTCTGCCATGTTCAGGGGTAAAATGAGCACCA
AGGAAGTTGACGAGCAGATGATCAATGTTCGAAACAAATGCTCTTCCTAC
TTTGTGGATTGGATTCCAAACAATGTGAAGTTAGTGTTTGTGACATTCCT
CCCAGAGGTCTCTCCATGGTATCCACCTTCTTCGGGAACTCAACATCCAT
CCAAAAAATGTTCCGAAGAGTGAGTGAGCAATTCAAAGCTATGTTTAGGA
GAAAGGCCTTCCTTCACTGGTACCTGCCCGGGCGGCCGCTCGAA
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