GE213243 | Citrus Genome Database

Overview


Name	GE213243
Unique Name	GE213243
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	394

Libraries


Library Name	Type
BH (somaclonal variated plants of Skaggs Bonanza navel orange) SSH library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB2B_HUMAN (Tubulin beta-2B chain OS=Homo sapiens GN=TUBB2B PE=1 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.095e-39
Identity = 45/58 (77.59%), Postives = 53/58 (91.38%), Query Frame = 3

Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVK 230
            M+D+KNMM A DPR GRYLTV+A+FRG+MS KEVDEQM+NV+NK SSYFV+WIPNNVK
Sbjct:  293 MFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK 350

HSP 2 Score: 84.7297 bits (208), Expect = 3.095e-39
Identity = 36/49 (73.47%), Postives = 44/49 (89.80%), Query Frame = 2

Query:  227 EVSVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            + +VCDIPPRGL M +TF GNST+IQ++F+R+SEQF AMFRRKAFLHWY
Sbjct:  350 KTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWY 398

BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB2B_BOVIN (Tubulin beta-2B chain OS=Bos taurus GN=TUBB2B PE=1 SV=2)

HSP 1 Score: 98.9821 bits (245), Expect = 3.095e-39
Identity = 45/58 (77.59%), Postives = 53/58 (91.38%), Query Frame = 3

Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVK 230
            M+D+KNMM A DPR GRYLTV+A+FRG+MS KEVDEQM+NV+NK SSYFV+WIPNNVK
Sbjct:  293 MFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK 350

HSP 2 Score: 84.7297 bits (208), Expect = 3.095e-39
Identity = 36/49 (73.47%), Postives = 44/49 (89.80%), Query Frame = 2

Query:  227 EVSVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            + +VCDIPPRGL M +TF GNST+IQ++F+R+SEQF AMFRRKAFLHWY
Sbjct:  350 KTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWY 398

BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB2A_RAT (Tubulin beta-2A chain OS=Rattus norvegicus GN=Tubb2a PE=1 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.095e-39
Identity = 45/58 (77.59%), Postives = 53/58 (91.38%), Query Frame = 3

Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVK 230
            M+D+KNMM A DPR GRYLTV+A+FRG+MS KEVDEQM+NV+NK SSYFV+WIPNNVK
Sbjct:  293 MFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK 350

HSP 2 Score: 84.7297 bits (208), Expect = 3.095e-39
Identity = 36/49 (73.47%), Postives = 44/49 (89.80%), Query Frame = 2

Query:  227 EVSVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            + +VCDIPPRGL M +TF GNST+IQ++F+R+SEQF AMFRRKAFLHWY
Sbjct:  350 KTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWY 398

BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB2A_MOUSE (Tubulin beta-2A chain OS=Mus musculus GN=Tubb2a PE=1 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.095e-39
Identity = 45/58 (77.59%), Postives = 53/58 (91.38%), Query Frame = 3

Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVK 230
            M+D+KNMM A DPR GRYLTV+A+FRG+MS KEVDEQM+NV+NK SSYFV+WIPNNVK
Sbjct:  293 MFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK 350

HSP 2 Score: 84.7297 bits (208), Expect = 3.095e-39
Identity = 36/49 (73.47%), Postives = 44/49 (89.80%), Query Frame = 2

Query:  227 EVSVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            + +VCDIPPRGL M +TF GNST+IQ++F+R+SEQF AMFRRKAFLHWY
Sbjct:  350 KTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWY 398

BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB2A_MACFA (Tubulin beta-2A chain OS=Macaca fascicularis GN=TUBB2A PE=2 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.095e-39
Identity = 45/58 (77.59%), Postives = 53/58 (91.38%), Query Frame = 3

Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVK 230
            M+D+KNMM A DPR GRYLTV+A+FRG+MS KEVDEQM+NV+NK SSYFV+WIPNNVK
Sbjct:  293 MFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK 350

HSP 2 Score: 84.7297 bits (208), Expect = 3.095e-39
Identity = 36/49 (73.47%), Postives = 44/49 (89.80%), Query Frame = 2

Query:  227 EVSVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            + +VCDIPPRGL M +TF GNST+IQ++F+R+SEQF AMFRRKAFLHWY
Sbjct:  350 KTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWY 398

BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB2A_HUMAN (Tubulin beta-2A chain OS=Homo sapiens GN=TUBB2A PE=1 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.095e-39
Identity = 45/58 (77.59%), Postives = 53/58 (91.38%), Query Frame = 3

Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVK 230
            M+D+KNMM A DPR GRYLTV+A+FRG+MS KEVDEQM+NV+NK SSYFV+WIPNNVK
Sbjct:  293 MFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK 350

HSP 2 Score: 84.7297 bits (208), Expect = 3.095e-39
Identity = 36/49 (73.47%), Postives = 44/49 (89.80%), Query Frame = 2

Query:  227 EVSVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            + +VCDIPPRGL M +TF GNST+IQ++F+R+SEQF AMFRRKAFLHWY
Sbjct:  350 KTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWY 398

BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB1_CHICK (Tubulin beta-1 chain OS=Gallus gallus PE=2 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.095e-39
Identity = 45/58 (77.59%), Postives = 53/58 (91.38%), Query Frame = 3

Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVK 230
            M+D+KNMM A DPR GRYLTV+A+FRG+MS KEVDEQM+NV+NK SSYFV+WIPNNVK
Sbjct:  293 MFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK 350

HSP 2 Score: 84.7297 bits (208), Expect = 3.095e-39
Identity = 36/49 (73.47%), Postives = 44/49 (89.80%), Query Frame = 2

Query:  227 EVSVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            + +VCDIPPRGL M +TF GNST+IQ++F+R+SEQF AMFRRKAFLHWY
Sbjct:  350 KTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWY 398

BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB_TRYBR (Tubulin beta chain OS=Trypanosoma brucei rhodesiense PE=3 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 3.095e-39
Identity = 46/58 (79.31%), Postives = 54/58 (93.10%), Query Frame = 3

Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVK 230
            M+DAKNMM AADPR GRYLT SA+FRG+MSTKEVDEQM+NV+NK SSYF++WIPNN+K
Sbjct:  293 MFDAKNMMQAADPRHGRYLTASALFRGRMSTKEVDEQMLNVQNKNSSYFIEWIPNNIK 350

HSP 2 Score: 81.2629 bits (199), Expect = 3.095e-39
Identity = 36/47 (76.60%), Postives = 39/47 (82.98%), Query Frame = 2

Query:  233 SVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            SVCDIPP+GL M  TF GN+T IQ+MFRRV EQF  MFRRKAFLHWY
Sbjct:  352 SVCDIPPKGLKMAVTFIGNNTCIQEMFRRVGEQFTLMFRRKAFLHWY 398

BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB7_CHICK (Tubulin beta-7 chain OS=Gallus gallus PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 5.253e-39
Identity = 45/58 (77.59%), Postives = 53/58 (91.38%), Query Frame = 3

Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVK 230
            ++DAKNMM A DPR GRYLTV+A+FRG+MS KEVDEQM+NV+NK SSYFV+WIPNNVK
Sbjct:  293 VFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK 350

HSP 2 Score: 84.3445 bits (207), Expect = 5.253e-39
Identity = 36/49 (73.47%), Postives = 43/49 (87.76%), Query Frame = 2

Query:  227 EVSVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            + +VCDIPPRGL M  TF GNST+IQ++F+R+SEQF AMFRRKAFLHWY
Sbjct:  350 KTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWY 398

BLAST of GE213243 vs. ExPASy Swiss-Prot
Match: TBB5_XENTR (Tubulin beta chain OS=Xenopus tropicalis GN=tubb PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 5.253e-39
Identity = 45/58 (77.59%), Postives = 53/58 (91.38%), Query Frame = 3

Query:   57 MWDAKNMMGAADPRFGRYLTVSAMFRGKMSTKEVDEQMINVRNKCSSYFVDWIPNNVK 230
            ++DAKNMM A DPR GRYLTV+A+FRG+MS KEVDEQM+NV+NK SSYFV+WIPNNVK
Sbjct:  293 VFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK 350

HSP 2 Score: 84.3445 bits (207), Expect = 5.253e-39
Identity = 36/49 (73.47%), Postives = 43/49 (87.76%), Query Frame = 2

Query:  227 EVSVCDIPPRGLSMVSTFFGNSTSIQKMFRRVSEQFKAMFRRKAFLHWY 373
            + +VCDIPPRGL M  TF GNST+IQ++F+R+SEQF AMFRRKAFLHWY
Sbjct:  350 KTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWY 398

The following BLAST results are available for this feature:

BLAST of GE213243 vs. ExPASy Swiss-Prot

BLAST of GE213243 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 238


Match Name	E-value	Identity	Description
TBB2B_HUMAN	3.095e-39	77.59	Tubulin beta-2B chain OS=Homo sapiens GN=TUBB2B PE...	[more]
TBB2B_BOVIN	3.095e-39	77.59	Tubulin beta-2B chain OS=Bos taurus GN=TUBB2B PE=1...	[more]
TBB2A_RAT	3.095e-39	77.59	Tubulin beta-2A chain OS=Rattus norvegicus GN=Tubb...	[more]
TBB2A_MOUSE	3.095e-39	77.59	Tubulin beta-2A chain OS=Mus musculus GN=Tubb2a PE...	[more]
TBB2A_MACFA	3.095e-39	77.59	Tubulin beta-2A chain OS=Macaca fascicularis GN=TU...	[more]
TBB2A_HUMAN	3.095e-39	77.59	Tubulin beta-2A chain OS=Homo sapiens GN=TUBB2A PE...	[more]
TBB1_CHICK	3.095e-39	77.59	Tubulin beta-1 chain OS=Gallus gallus PE=2 SV=1	[more]
TBB_TRYBR	3.095e-39	79.31	Tubulin beta chain OS=Trypanosoma brucei rhodesien...	[more]
TBB7_CHICK	5.253e-39	77.59	Tubulin beta-7 chain OS=Gallus gallus PE=2 SV=1	[more]
TBB5_XENTR	5.253e-39	77.59	Tubulin beta chain OS=Xenopus tropicalis GN=tubb P...	[more]

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Properties


Property Name	Value
Genbank description	92 BH (somaclonal variated plants of Skaggs Bonanza navel orange) SSH library Citrus sinensis cDNA 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GE213243 ID=GE213243; Name=GE213243; organism=Citrus sinensis; type=EST; length=394bp
ATTACCAGGGTCCCGGCCGAGGTACCGTGCCTTGATTTTTTTTTTTTTTT
TTTTTTATGTGGGATGCCAAGAACATGATGGGTGCTGCCGACCCAAGGTT
TGGTCGTTACCTCACTGTTTCTGCCATGTTCAGGGGTAAAATGAGCACCA
AGGAAGTTGACGAGCAGATGATCAATGTTCGAAACAAATGCTCTTCCTAC
TTTGTGGATTGGATTCCAAACAATGTGAAGTTAGTGTTTGTGACATTCCT
CCCAGAGGTCTCTCCATGGTATCCACCTTCTTCGGGAACTCAACATCCAT
CCAAAAAATGTTCCGAAGAGTGAGTGAGCAATTCAAAGCTATGTTTAGGA
GAAAGGCCTTCCTTCACTGGTACCTGCCCGGGCGGCCGCTCGAA