EY753864

Overview
NameEY753864
Unique NameEY753864
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length911
Libraries
Library NameType
Sweet orange leaf, field plant Acdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY753864 vs. ExPASy Swiss-Prot
Match: HIS7_BURVG (Imidazoleglycerol-phosphate dehydratase OS=Burkholderia vietnamiensis (strain G4 / LMG 22486) GN=hisB PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 2.115e-38
Identity = 65/125 (52.00%), Postives = 86/125 (68.80%), Query Frame = 2 
Query:  281 RIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRAHLNYDLQIPHXRV 655
            R+ EV R T ET + VK+++DG+G    +T +PFLDHMLDQ+A HGL D+ V A GD HIDDHHT EDVG+ +G A+ +A+GD+KGI R+G    PLDEAL  V +D SGR  L + +     R+
Sbjct:    2 RVAEVVRNTSETQIRVKLDLDGTGRQKLATGVPFLDHMLDQIARHGLIDLEVEAHGDTHIDDHHTVEDVGITLGQAVAKAVGDKKGIRRYGHSYVPLDEALSRVVIDFSGRPGLEFHVPFTRARI 126

HSP 2 Score: 43.5134 bits (101), Expect = 2.115e-38
Identity = 22/49 (44.90%), Postives = 28/49 (57.14%), Query Frame = 1 
Query:  655 GTYDTQLVEHFFQSLVNTSGMTLHIRQLAGKXSHHIIGAPFKLLLGALR 801
            GT+D  L   FF+  VN +G+TLHI  L G  +HH +   FK    ALR
Sbjct:  127 GTFDVDLSIEFFRGFVNHAGVTLHIDNLRGINAHHQLETVFKAFGRALR 175
[Close]
BLAST of EY753864 vs. ExPASy Swiss-Prot
Match: HIS7_BURM1 (Imidazoleglycerol-phosphate dehydratase OS=Burkholderia multivorans (strain ATCC 17616 / 249) GN=hisB PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 2.115e-38
Identity = 65/125 (52.00%), Postives = 86/125 (68.80%), Query Frame = 2 
Query:  281 RIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRAHLNYDLQIPHXRV 655
            R+ EV R T ET + VK+++DG+G    +T +PFLDHMLDQ+A HGL D+ V A GD HIDDHHT EDVG+ +G A+ +A+GD+KGI R+G    PLDEAL  V +D SGR  L + +     R+
Sbjct:    2 RVAEVVRNTSETQIRVKLDLDGTGRQKLATGVPFLDHMLDQIARHGLVDLEVEAHGDTHIDDHHTVEDVGITLGQAVAKAIGDKKGIRRYGHAYVPLDEALSRVVIDFSGRPGLEFHVPFTRARI 126

HSP 2 Score: 43.5134 bits (101), Expect = 2.115e-38
Identity = 22/49 (44.90%), Postives = 28/49 (57.14%), Query Frame = 1 
Query:  655 GTYDTQLVEHFFQSLVNTSGMTLHIRQLAGKXSHHIIGAPFKLLLGALR 801
            GT+D  L   FF+  VN +G+TLHI  L G  +HH +   FK    ALR
Sbjct:  127 GTFDVDLSIEFFRGFVNHAGVTLHIDNLRGINAHHQLETVFKAFGRALR 175
[Close]
BLAST of EY753864 vs. ExPASy Swiss-Prot
Match: HIS7_BURTA (Imidazoleglycerol-phosphate dehydratase OS=Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) GN=hisB PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 2.115e-38
Identity = 65/125 (52.00%), Postives = 86/125 (68.80%), Query Frame = 2 
Query:  281 RIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRAHLNYDLQIPHXRV 655
            R+ EV R T ET + VKI++DG+G    +T +PFLDHMLDQ+A HGL D+ + A GD HIDDHHT EDVG+ +G A+ +A+GD+KGI R+G    PLDEAL  V +D SGR  L + +     R+
Sbjct:    2 RVAEVVRNTSETQIRVKIDLDGTGRQKLATGVPFLDHMLDQIARHGLVDLDIEAHGDTHIDDHHTVEDVGITLGQAVAKAVGDKKGIRRYGHSYVPLDEALSRVVIDFSGRPGLEFHVPFTRARI 126

HSP 2 Score: 43.8986 bits (102), Expect = 2.115e-38
Identity = 22/49 (44.90%), Postives = 28/49 (57.14%), Query Frame = 1 
Query:  655 GTYDTQLVEHFFQSLVNTSGMTLHIRQLAGKXSHHIIGAPFKLLLGALR 801
            GT+D  L   FF+  VN +G+TLHI  L G  +HH +   FK    ALR
Sbjct:  127 GTFDVDLSIEFFRGFVNHAGVTLHIDNLRGVNAHHQLETVFKAFGRALR 175
[Close]
BLAST of EY753864 vs. ExPASy Swiss-Prot
Match: HIS7_PSYWF (Imidazoleglycerol-phosphate dehydratase OS=Psychrobacter sp. (strain PRwf-1) GN=hisB PE=3 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 2.752e-38
Identity = 64/125 (51.20%), Postives = 84/125 (67.20%), Query Frame = 2 
Query:  281 RIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRAHLNYDLQIPHXRV 655
            RI  V+R T ET VSV +N+DG+G     T +PFLDHMLDQ+  HGLFD+ +   GD +IDDHH+ ED G+ +G A  +ALGD+KGI R+G F APLDE+L    +D+SGR  L+ D+      V
Sbjct:   15 RIASVERNTAETQVSVTVNLDGTGQGKIDTGVPFLDHMLDQIKRHGLFDLDITCKGDTYIDDHHSIEDTGITLGQAFAKALGDKKGIRRYGHFYAPLDESLTRAVVDISGRPGLHMDIPFTRSHV 139

HSP 2 Score: 44.669 bits (104), Expect = 2.752e-38
Identity = 22/49 (44.90%), Postives = 26/49 (53.06%), Query Frame = 1 
Query:  655 GTYDTQLVEHFFQSLVNTSGMTLHIRQLAGKXSHHIIGAPFKLLLGALR 801
            G +D  L   FF   VN + MT+H+  L GK SHH I   FK    ALR
Sbjct:  140 GNFDVDLFSEFFFGFVNHAWMTVHLDNLKGKNSHHQIECTFKAFARALR 188
[Close]
BLAST of EY753864 vs. ExPASy Swiss-Prot
Match: HIS7_RALME (Imidazoleglycerol-phosphate dehydratase OS=Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839) GN=hisB PE=3 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 2.756e-38
Identity = 65/125 (52.00%), Postives = 86/125 (68.80%), Query Frame = 2 
Query:  281 RIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRAHLNYDLQIPHXRV 655
            R+ EV R T ET + V +N+DG+G    ++ +PFLDHMLDQ+A HG+FD+ V ATGD HIDDHHT EDVG+ +G A+ +A+GD+KGI R+G    PLDE L  V +D SGR  L + +     RV
Sbjct:    2 RVAEVTRNTSETQIRVSLNLDGTGRQKLASGVPFLDHMLDQIARHGMFDLEVEATGDTHIDDHHTVEDVGITLGQAVARAIGDKKGITRYGHSYVPLDECLSRVVIDFSGRPGLEFHVPFTRARV 126

HSP 2 Score: 40.817 bits (94), Expect = 2.756e-38
Identity = 20/49 (40.82%), Postives = 27/49 (55.10%), Query Frame = 1 
Query:  655 GTYDTQLVEHFFQSLVNTSGMTLHIRQLAGKXSHHIIGAPFKLLLGALR 801
            G++D  L   FF+  VN +G+TLHI  + G  +HH     FK    ALR
Sbjct:  127 GSFDVDLTIEFFRGFVNHAGVTLHIDNIRGINAHHQCETVFKAFGRALR 175
[Close]
BLAST of EY753864 vs. ExPASy Swiss-Prot
Match: HIS7_MARAV (Imidazoleglycerol-phosphate dehydratase OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=hisB PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 3.589e-38
Identity = 65/124 (52.42%), Postives = 88/124 (70.97%), Query Frame = 2 
Query:  281 RIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRAHLNYDLQIPHXR 652
            R   V+R T ET ++V+IN+DG+G A+  T +PFL+HM+DQ+A HGL D+ +   GD+HIDDHHT ED+G+ +G A  QA+GD+KGI R+G    PLDEAL  V +DLSGR  L  D  +P+ R
Sbjct:    4 RKARVERNTLETQITVEINLDGTGKANFDTGVPFLEHMMDQIARHGLVDLDITCKGDLHIDDHHTVEDIGITLGQAFKQAVGDKKGIRRYGHAYVPLDEALSRVVIDLSGRPGLLMD--VPYTR 125

HSP 2 Score: 45.0542 bits (105), Expect = 3.589e-38
Identity = 24/49 (48.98%), Postives = 27/49 (55.10%), Query Frame = 1 
Query:  655 GTYDTQLVEHFFQSLVNTSGMTLHIRQLAGKXSHHIIGAPFKLLLGALR 801
            G +D  L   FFQ  VN S +TLHI  L GK +HH I   FK    ALR
Sbjct:  129 GGFDVDLFAEFFQGFVNHSMVTLHIDNLKGKNTHHQIETVFKAFGRALR 177
[Close]
BLAST of EY753864 vs. ExPASy Swiss-Prot
Match: HIS7_RHIEC (Imidazoleglycerol-phosphate dehydratase OS=Rhizobium etli (strain CFN 42 / ATCC 51251) GN=hisB PE=3 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 4.674e-38
Identity = 59/120 (49.17%), Postives = 83/120 (69.17%), Query Frame = 2 
Query:  275 SGRIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRAHLNYDL 634
            + R G V R T ET++SV +N+DG+G +  ST + F DHMLDQL+ H L D+ + A GD+HIDDHHT ED G+AIG A+ +ALGDR+GI R+      +DE +   ++DLSGR  L +++
Sbjct:    6 ASRTGSVARKTNETSISVSVNLDGTGKSKISTGVGFFDHMLDQLSRHSLIDMEIEAKGDLHIDDHHTVEDTGIAIGQAISKALGDRRGITRYASIDLAMDETMTKAAVDLSGRPFLVWNV 125

HSP 2 Score: 50.8322 bits (120), Expect = 4.674e-38
Identity = 25/56 (44.64%), Postives = 34/56 (60.71%), Query Frame = 1 
Query:  634 ANSTXESGTYDTQLVEHFFQSLVNTSGMTLHIRQLAGKXSHHIIGAPFKLLLGALR 801
            A S  + GT+DT+LV  FFQ+L   +G+TLH+    G  +HHI    FK +  ALR
Sbjct:  126 AFSAPKIGTFDTELVREFFQALAQNAGITLHLLNHYGANNHHIAETCFKAVARALR 181
[Close]
BLAST of EY753864 vs. ExPASy Swiss-Prot
Match: HIS7_RHILW (Imidazoleglycerol-phosphate dehydratase OS=Rhizobium leguminosarum bv. trifolii (strain WSM2304) GN=hisB PE=3 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 4.674e-38
Identity = 58/120 (48.33%), Postives = 83/120 (69.17%), Query Frame = 2 
Query:  275 SGRIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRAHLNYDL 634
            + R G V R T ET++SV +N+DG+G +  ST + F DHMLDQL+ H L D+ + + GD+HIDDHHT ED G+AIG A+ +ALGDR+GI R+      +DE +   ++DLSGR  L +++
Sbjct:    6 ASRTGSVSRKTNETSISVSVNLDGTGKSKISTGVGFFDHMLDQLSRHSLIDMEIESQGDLHIDDHHTVEDTGIAIGQAISKALGDRRGITRYASIDLAMDETMTKAAVDLSGRPFLVWNV 125

HSP 2 Score: 51.6026 bits (122), Expect = 4.674e-38
Identity = 26/56 (46.43%), Postives = 34/56 (60.71%), Query Frame = 1 
Query:  634 ANSTXESGTYDTQLVEHFFQSLVNTSGMTLHIRQLAGKXSHHIIGAPFKLLLGALR 801
            A S  + GT+DT+LV  FFQ+L   +G+TLHI    G  +HHI    FK +  ALR
Sbjct:  126 AFSAPKIGTFDTELVREFFQALAQNAGITLHILNHYGANNHHIAETCFKAVARALR 181
[Close]
BLAST of EY753864 vs. ExPASy Swiss-Prot
Match: HIS7_OLICO (Imidazoleglycerol-phosphate dehydratase OS=Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) GN=hisB PE=3 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 4.675e-38
Identity = 60/125 (48.00%), Postives = 85/125 (68.00%), Query Frame = 2 
Query:  281 RIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRAHLNYDLQIPHXRV 655
            R   +KR TKET+++V +N+DG+G +D +T I F DHMLD LA H   D+ V+A GD+H+D HHT EDVG+A+G A+ QALGD  GI R+     P+DE L  V +D+SGR  L + ++ P  ++
Sbjct:    2 RTATIKRKTKETDIAVSVNLDGTGASDVATGIGFFDHMLDLLARHSGIDITVKANGDLHVDHHHTTEDVGIALGQAVKQALGDMAGITRYAGVHLPMDETLSRVVIDISGRPVLVFKVEFPRDKI 126

HSP 2 Score: 48.521 bits (114), Expect = 4.675e-38
Identity = 23/49 (46.94%), Postives = 32/49 (65.31%), Query Frame = 1 
Query:  655 GTYDTQLVEHFFQSLVNTSGMTLHIRQLAGKXSHHIIGAPFKLLLGALR 801
            G +DT+LV  +FQ+    +G+TLH+  L G+ SHHI  + FK L  ALR
Sbjct:  127 GEFDTELVREWFQAFAMNAGITLHVETLYGENSHHISESCFKGLARALR 175
[Close]
BLAST of EY753864 vs. ExPASy Swiss-Prot
Match: HIS7_THEP3 (Imidazoleglycerol-phosphate dehydratase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) GN=hisB PE=3 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 4.677e-38
Identity = 66/125 (52.80%), Postives = 85/125 (68.00%), Query Frame = 2 
Query:  281 RIGEVKRVTKETNVSVKINVDGSGVADSSTCIPFLDHMLDQLASHGLFDVHVRATGDIHIDDHHTNEDVGLAIGTALLQALGDRKGINRFGDFTAPLDEALIHVSLDLSGRAHLNYDLQIPHXRV 655
            R  EVKR T+ET++ V++N+DG G  D +T I F DHML   A HGLFD+ V A GD+ +D HHT EDVG+ +G A L+A GD+K I RF  F  P+DEAL+ VS+D+SGR  L  DL +   RV
Sbjct:    2 RKAEVKRKTRETDIYVELNIDGKGNYDIATGIGFFDHMLSLFAKHGLFDLKVVAKGDLEVDTHHTVEDVGIVLGNAFLKAAGDKKSIKRFSTFYVPMDEALVRVSVDISGRPFLYCDLPLKAERV 126

HSP 2 Score: 45.0542 bits (105), Expect = 4.677e-38
Identity = 22/48 (45.83%), Postives = 29/48 (60.42%), Query Frame = 1 
Query:  655 GTYDTQLVEHFFQSLVNTSGMTLHIRQLAGKXSHHIIGAPFKLLLGAL 798
            G ++T+ VE F ++     G+TLH+  L G  SHHII A FK L  AL
Sbjct:  127 GNFETENVEEFLRAFAYNFGITLHVELLHGSNSHHIIEATFKALGRAL 174
The following BLAST results are available for this feature:
BLAST of EY753864 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
HIS7_BURVG2.115e-3852.00Imidazoleglycerol-phosphate dehydratase OS=Burkhol... [more]
HIS7_BURM12.115e-3852.00Imidazoleglycerol-phosphate dehydratase OS=Burkhol... [more]
HIS7_BURTA2.115e-3852.00Imidazoleglycerol-phosphate dehydratase OS=Burkhol... [more]
HIS7_PSYWF2.752e-3851.20Imidazoleglycerol-phosphate dehydratase OS=Psychro... [more]
HIS7_RALME2.756e-3852.00Imidazoleglycerol-phosphate dehydratase OS=Ralston... [more]
HIS7_MARAV3.589e-3852.42Imidazoleglycerol-phosphate dehydratase OS=Marinob... [more]
HIS7_RHIEC4.674e-3849.17Imidazoleglycerol-phosphate dehydratase OS=Rhizobi... [more]
HIS7_RHILW4.674e-3848.33Imidazoleglycerol-phosphate dehydratase OS=Rhizobi... [more]
HIS7_OLICO4.675e-3848.00Imidazoleglycerol-phosphate dehydratase OS=Oligotr... [more]
HIS7_THEP34.677e-3852.80Imidazoleglycerol-phosphate dehydratase OS=Thermoa... [more]

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Properties
Property NameValue
Genbank descriptionCS12-C1-001-007-G10-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY753864 ID=EY753864; Name=EY753864; organism=Citrus sinensis; type=EST; length=911bp
TGCTACTCTTTTTGCGACATTGGAAACGATAGTGTGCAAAATGGAGCTCT
CGGTCCCAGCTCGCCTTGTTCACCTTTCAATCTCGTCCACGTCCTCACGC
CGGCTCCTCGTTAAACCAAAAACCAATCTCTCTCGTAAACTTCTCCCCAT
CTCCACACAATACTGCTCCTCAACGCGCCGCATGAACTCCCTCACAACAC
CACGCGCCTCTCTAAACCCAGACGGCGACAGCAAACACAACAACGGGTCC
GCCTCAACTTCCTCTGCCGTCGATTCTGGTCGAATTGGGGAAGTGAAAAG
AGTCACGAAAGAGACCAACGTTTCTGTGAAGATTAACGTCGATGGCTCTG
GCGTTGCTGATTCATCCACTTGCATTCCCTTCCTAGATCACATGCTTGAT
CAACTGGCTTCGCACGGGCTATTTGATGTGCACGTGAGGGCAACCGGAGA
CATTCACATTGATGATCATCATACCAATGAAGACGTTGGGCTTGCCATTG
GCACGGCATTGCTGCAGGCTCTTGGTGATAGGAAAGGAATTAATCGGTTT
GGTGATTTTACAGCTCCTCTTGATGAAGCTTTGATTCATGTTTCATTGGA
TTTATCTGGACGGGCACATTTAAATTATGATTTGCAAATTCCACACANGA
GAGTGGAACTTACGACACTCAATTGGTGGAGCACTTTTTCCAGTCTTTGG
TGAACACTTCTGGAATGACTCTTCACATTCGACAGCTTGCTGGGAAAAAN
TCTCATCACATTATTGGAGCACCTTTCAAGCTTTTGCTAGGGGCTCTCCG
ACAGCACAGNATATGATCCACGTCGCCGTGGACTATACAAGCTCAAAGGG
GTCTACTACGATCTTGAACTTACGGGATCATGGACGGGGGATGGGGTCTC
ATGTAGCCTAA
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