EY753834

Overview
NameEY753834
Unique NameEY753834
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length896
Libraries
Library NameType
Sweet orange leaf, field plant Acdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY753834 vs. ExPASy Swiss-Prot
Match: ACTB_TRIVU (Actin, cytoplasmic 1 OS=Trichosurus vulpecula GN=ACTB PE=1 SV=1)

HSP 1 Score: 420.239 bits (1079), Expect = 7.688e-117
Identity = 207/237 (87.34%), Postives = 217/237 (91.56%), Query Frame = 2 
Query:  113 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYXGYALPHAILRLDLAGRDLTDALMKILTEXSYMFTTTAEREIVRDMKEKLAYVALDYEPELETAXKQLICEKT 823
            +DI  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV+DSGDGV+HTVPIY GYALPHAILRLDLAGRDLTD LMKILTE  Y FTTTAEREIVRD+KEKL YVALD+E E+ TA      EK+
Sbjct:    3 DDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKS 239
[Close]
BLAST of EY753834 vs. ExPASy Swiss-Prot
Match: ACTB_SPECI (Actin, cytoplasmic 1 OS=Spermophilus citellus GN=ACTB PE=2 SV=1)

HSP 1 Score: 420.239 bits (1079), Expect = 7.688e-117
Identity = 207/237 (87.34%), Postives = 217/237 (91.56%), Query Frame = 2 
Query:  113 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYXGYALPHAILRLDLAGRDLTDALMKILTEXSYMFTTTAEREIVRDMKEKLAYVALDYEPELETAXKQLICEKT 823
            +DI  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV+DSGDGV+HTVPIY GYALPHAILRLDLAGRDLTD LMKILTE  Y FTTTAEREIVRD+KEKL YVALD+E E+ TA      EK+
Sbjct:    3 DDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKS 239
[Close]
BLAST of EY753834 vs. ExPASy Swiss-Prot
Match: ACTB_SIGHI (Actin, cytoplasmic 1 OS=Sigmodon hispidus GN=ACTB PE=2 SV=1)

HSP 1 Score: 420.239 bits (1079), Expect = 7.688e-117
Identity = 207/237 (87.34%), Postives = 217/237 (91.56%), Query Frame = 2 
Query:  113 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYXGYALPHAILRLDLAGRDLTDALMKILTEXSYMFTTTAEREIVRDMKEKLAYVALDYEPELETAXKQLICEKT 823
            +DI  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV+DSGDGV+HTVPIY GYALPHAILRLDLAGRDLTD LMKILTE  Y FTTTAEREIVRD+KEKL YVALD+E E+ TA      EK+
Sbjct:    3 DDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKS 239
[Close]
BLAST of EY753834 vs. ExPASy Swiss-Prot
Match: ACTB_SHEEP (Actin, cytoplasmic 1 OS=Ovis aries GN=ACTB PE=2 SV=1)

HSP 1 Score: 420.239 bits (1079), Expect = 7.688e-117
Identity = 207/237 (87.34%), Postives = 217/237 (91.56%), Query Frame = 2 
Query:  113 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYXGYALPHAILRLDLAGRDLTDALMKILTEXSYMFTTTAEREIVRDMKEKLAYVALDYEPELETAXKQLICEKT 823
            +DI  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV+DSGDGV+HTVPIY GYALPHAILRLDLAGRDLTD LMKILTE  Y FTTTAEREIVRD+KEKL YVALD+E E+ TA      EK+
Sbjct:    3 DDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKS 239
[Close]
BLAST of EY753834 vs. ExPASy Swiss-Prot
Match: ACTB_RAT (Actin, cytoplasmic 1 OS=Rattus norvegicus GN=Actb PE=1 SV=1)

HSP 1 Score: 420.239 bits (1079), Expect = 7.688e-117
Identity = 207/237 (87.34%), Postives = 217/237 (91.56%), Query Frame = 2 
Query:  113 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYXGYALPHAILRLDLAGRDLTDALMKILTEXSYMFTTTAEREIVRDMKEKLAYVALDYEPELETAXKQLICEKT 823
            +DI  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV+DSGDGV+HTVPIY GYALPHAILRLDLAGRDLTD LMKILTE  Y FTTTAEREIVRD+KEKL YVALD+E E+ TA      EK+
Sbjct:    3 DDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKS 239
[Close]
BLAST of EY753834 vs. ExPASy Swiss-Prot
Match: ACTB_PONAB (Actin, cytoplasmic 1 OS=Pongo abelii GN=ACTB PE=2 SV=1)

HSP 1 Score: 420.239 bits (1079), Expect = 7.688e-117
Identity = 207/237 (87.34%), Postives = 217/237 (91.56%), Query Frame = 2 
Query:  113 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYXGYALPHAILRLDLAGRDLTDALMKILTEXSYMFTTTAEREIVRDMKEKLAYVALDYEPELETAXKQLICEKT 823
            +DI  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV+DSGDGV+HTVPIY GYALPHAILRLDLAGRDLTD LMKILTE  Y FTTTAEREIVRD+KEKL YVALD+E E+ TA      EK+
Sbjct:    3 DDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKS 239
[Close]
BLAST of EY753834 vs. ExPASy Swiss-Prot
Match: ACTB_PIG (Actin, cytoplasmic 1 OS=Sus scrofa GN=ACTB PE=2 SV=2)

HSP 1 Score: 420.239 bits (1079), Expect = 7.688e-117
Identity = 207/237 (87.34%), Postives = 217/237 (91.56%), Query Frame = 2 
Query:  113 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYXGYALPHAILRLDLAGRDLTDALMKILTEXSYMFTTTAEREIVRDMKEKLAYVALDYEPELETAXKQLICEKT 823
            +DI  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV+DSGDGV+HTVPIY GYALPHAILRLDLAGRDLTD LMKILTE  Y FTTTAEREIVRD+KEKL YVALD+E E+ TA      EK+
Sbjct:    3 DDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKS 239
[Close]
BLAST of EY753834 vs. ExPASy Swiss-Prot
Match: ACTB_PANTR (Actin, cytoplasmic 1 OS=Pan troglodytes GN=ACTB PE=2 SV=1)

HSP 1 Score: 420.239 bits (1079), Expect = 7.688e-117
Identity = 207/237 (87.34%), Postives = 217/237 (91.56%), Query Frame = 2 
Query:  113 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYXGYALPHAILRLDLAGRDLTDALMKILTEXSYMFTTTAEREIVRDMKEKLAYVALDYEPELETAXKQLICEKT 823
            +DI  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV+DSGDGV+HTVPIY GYALPHAILRLDLAGRDLTD LMKILTE  Y FTTTAEREIVRD+KEKL YVALD+E E+ TA      EK+
Sbjct:    3 DDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKS 239
[Close]
BLAST of EY753834 vs. ExPASy Swiss-Prot
Match: ACTB_MOUSE (Actin, cytoplasmic 1 OS=Mus musculus GN=Actb PE=1 SV=1)

HSP 1 Score: 420.239 bits (1079), Expect = 7.688e-117
Identity = 207/237 (87.34%), Postives = 217/237 (91.56%), Query Frame = 2 
Query:  113 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYXGYALPHAILRLDLAGRDLTDALMKILTEXSYMFTTTAEREIVRDMKEKLAYVALDYEPELETAXKQLICEKT 823
            +DI  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV+DSGDGV+HTVPIY GYALPHAILRLDLAGRDLTD LMKILTE  Y FTTTAEREIVRD+KEKL YVALD+E E+ TA      EK+
Sbjct:    3 DDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKS 239
[Close]
BLAST of EY753834 vs. ExPASy Swiss-Prot
Match: ACTB_MESAU (Actin, cytoplasmic 1 OS=Mesocricetus auratus GN=ACTB PE=2 SV=1)

HSP 1 Score: 420.239 bits (1079), Expect = 7.688e-117
Identity = 207/237 (87.34%), Postives = 217/237 (91.56%), Query Frame = 2 
Query:  113 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYXGYALPHAILRLDLAGRDLTDALMKILTEXSYMFTTTAEREIVRDMKEKLAYVALDYEPELETAXKQLICEKT 823
            +DI  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV+DSGDGV+HTVPIY GYALPHAILRLDLAGRDLTD LMKILTE  Y FTTTAEREIVRD+KEKL YVALD+E E+ TA      EK+
Sbjct:    3 DDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKS 239
The following BLAST results are available for this feature:
BLAST of EY753834 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 479
Match NameE-valueIdentityDescription
ACTB_TRIVU7.688e-11787.34Actin, cytoplasmic 1 OS=Trichosurus vulpecula GN=A... [more]
ACTB_SPECI7.688e-11787.34Actin, cytoplasmic 1 OS=Spermophilus citellus GN=A... [more]
ACTB_SIGHI7.688e-11787.34Actin, cytoplasmic 1 OS=Sigmodon hispidus GN=ACTB ... [more]
ACTB_SHEEP7.688e-11787.34Actin, cytoplasmic 1 OS=Ovis aries GN=ACTB PE=2 SV... [more]
ACTB_RAT7.688e-11787.34Actin, cytoplasmic 1 OS=Rattus norvegicus GN=Actb ... [more]
ACTB_PONAB7.688e-11787.34Actin, cytoplasmic 1 OS=Pongo abelii GN=ACTB PE=2 ... [more]
ACTB_PIG7.688e-11787.34Actin, cytoplasmic 1 OS=Sus scrofa GN=ACTB PE=2 SV... [more]
ACTB_PANTR7.688e-11787.34Actin, cytoplasmic 1 OS=Pan troglodytes GN=ACTB PE... [more]
ACTB_MOUSE7.688e-11787.34Actin, cytoplasmic 1 OS=Mus musculus GN=Actb PE=1 ... [more]
ACTB_MESAU7.688e-11787.34Actin, cytoplasmic 1 OS=Mesocricetus auratus GN=AC... [more]

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Properties
Property NameValue
Genbank descriptionCS12-C1-001-007-D12-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY753834 ID=EY753834; Name=EY753834; organism=Citrus sinensis; type=EST; length=896bp
CAGTACCATTTACAGAGCGATAGAGAGAATCGACACTCTCAGCTAGACTC
CATCTTCGAATTTTTCTCTCGTCGTCTCTTCAATCTAACTTTTATAAAAA
ATGGCCGATGCTGAGGATATTCAACCCCTTGTTTGTGACAATGGAACTGG
AATGGTGAAGGCTGGATTTGCTGGAGATGATGCTCCGAGGGCAGTATTCC
CTAGTATTGTTGGTAGGCCTCGACACACTGGAGTCATGGTTGGAATGGGG
CAGAAGGATGCCTATGTTGGTGATGAGGCCCAATCGAAAAGAGGTATTCT
TACTTTGAAATACCCAATTGAGCACGGTATTGTAAGCAACTGGGATGATA
TGGAAAAGATCTGGCATCATACCTTCTACAACGAGCTTCGTGTTGCTCCT
GAAGAGCACCCAGTGCTTCTCACTGAGGCACCTCTTAACCCCAAGGCCAA
CAGAGAGAAGATGACCCAAATTATGTTTGAAACTTTCAATGTGCCAGCCA
TGTACGTTGCCATCCAGGCCGTTCTATCTCTTTATGCCAGTGGTCGTACA
ACTGGTATTGTGCTGGATTCTGGTGATGGTGTGTCTCATACTGTACCAAT
CTATGANGGATATGCCCTTCCTCATGCCATTCTTCGTTTGGACTTGGCTG
GTCGTGATCTCACTGATGCATTGATGAAGATTCTTACTGAGANGAGTTAC
ATGTTTACCACCACAGCCGAACGGGAAATTGTCCGTGACATGAAGGAGAA
ACTTGCTTATGTTGCCCTAGACTATGAGCCAGAACTGGAGACTGCCNAGA
AGCAGCTCATCTGTGAGAAAACTATGAGCTGNCTGATGGCCAAACATCAC
CATGNAGCTGAAAGATCCGTGCCCAAAATTTCTGTTCAGCCATCTT
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