EY753720

Overview
NameEY753720
Unique NameEY753720
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length901
Libraries
Library NameType
Sweet orange leaf, field plant Acdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY753720 vs. ExPASy Swiss-Prot
Match: PROA_LEPBP (Gamma-glutamyl phosphate reductase OS=Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) GN=proA PE=3 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 8.935e-13
Identity = 32/104 (30.77%), Postives = 62/104 (59.62%), Query Frame = 3 
Query:  396 AREMAVADEKSSRRLQALQSQDRRKILLDVADALEANESLIKVENEADVAAAQEAGYAKSLVSRLALKPGKISSLAKSIRILADMEEPIGQILKRTELADGLIL 707
            A+ +A   +++SR L++L +  +  +L  V   L  NE+ I  EN+ D+    + G + +++ RL L   +I S+AKSI  + ++ +P+G++++ T L +GL L
Sbjct:    9 AKSIATKAKEASRALKSLTTLQKNSVLKRVETLLLENETAIIKENQIDLQNGIQKGLSSAMMDRLLLDSKRIQSMAKSIEEIRNLPDPVGEVVRGTILPNGLEL 112

HSP 2 Score: 34.2686 bits (77), Expect = 8.935e-13
Identity = 14/27 (51.85%), Postives = 21/27 (77.78%), Query Frame = 2 
Query:  725 LGVLLIVFESRPDALVQIASWAHSSGN 805
            +GV++ +FESRP+ +V IAS +  SGN
Sbjct:  119 IGVVMTIFESRPNVIVDIASLSFKSGN 145
[Close]
BLAST of EY753720 vs. ExPASy Swiss-Prot
Match: PROA_LEPBA (Gamma-glutamyl phosphate reductase OS=Leptospira biflexa serovar Patoc (strain Patoc 1 / Ames) GN=proA PE=3 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 8.935e-13
Identity = 32/104 (30.77%), Postives = 62/104 (59.62%), Query Frame = 3 
Query:  396 AREMAVADEKSSRRLQALQSQDRRKILLDVADALEANESLIKVENEADVAAAQEAGYAKSLVSRLALKPGKISSLAKSIRILADMEEPIGQILKRTELADGLIL 707
            A+ +A   +++SR L++L +  +  +L  V   L  NE+ I  EN+ D+    + G + +++ RL L   +I S+AKSI  + ++ +P+G++++ T L +GL L
Sbjct:    9 AKSIATKAKEASRALKSLTTLQKNSVLKRVETLLLENETAIIKENQIDLQNGIQKGLSSAMMDRLLLDSKRIQSMAKSIEEIRNLPDPVGEVVRGTILPNGLEL 112

HSP 2 Score: 34.2686 bits (77), Expect = 8.935e-13
Identity = 14/27 (51.85%), Postives = 21/27 (77.78%), Query Frame = 2 
Query:  725 LGVLLIVFESRPDALVQIASWAHSSGN 805
            +GV++ +FESRP+ +V IAS +  SGN
Sbjct:  119 IGVVMTIFESRPNVIVDIASLSFKSGN 145
[Close]
BLAST of EY753720 vs. ExPASy Swiss-Prot
Match: PROA_PROMH (Gamma-glutamyl phosphate reductase OS=Proteus mirabilis (strain HI4320) GN=proA PE=3 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 8.937e-13
Identity = 34/107 (31.78%), Postives = 62/107 (57.94%), Query Frame = 3 
Query:  402 EMAVADEKSSRRLQALQSQDRRKILLDVADALEANESLIKVENEADVAAAQEAGYAKSLVSRLALKPGKISSLAKSIRILADMEEPIGQILKRTELADGLILEKHHV 722
            +M  A  ++S  L  L ++ + + LL +AD LE  E++I   NE D+ AA+E+    +++ RL L   ++ ++A  +R +  +E+P+GQ++    L  GL LE+  V
Sbjct:    4 QMGKAAREASWHLAQLSTEQKNQALLVIADLLEQQEAIILAANEKDMVAARESNINAAMLDRLLLTSERLKAIADDVRQVCHLEDPVGQVIDGRLLDSGLRLERRRV 110

HSP 2 Score: 27.7202 bits (60), Expect = 8.937e-13
Identity = 10/27 (37.04%), Postives = 19/27 (70.37%), Query Frame = 2 
Query:  725 LGVLLIVFESRPDALVQIASWAHSSGN 805
            LGV+ +++E+RP+  + +AS    +GN
Sbjct:  112 LGVVGVIYEARPNVTIDVASLCLKTGN 138
[Close]
BLAST of EY753720 vs. ExPASy Swiss-Prot
Match: PROA_CARHZ (Gamma-glutamyl phosphate reductase OS=Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) GN=proA PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 8.939e-13
Identity = 30/101 (29.70%), Postives = 61/101 (60.40%), Query Frame = 3 
Query:  405 MAVADEKSSRRLQALQSQDRRKILLDVADALEANESLIKVENEADVAAAQEAGYAKSLVSRLALKPGKISSLAKSIRILADMEEPIGQILKRTELADGLIL 707
            +A   +++S++L  L ++ + + LL +A  LE N   I  EN+ D+A A++ G + + + RL L   +I  +A+ +R +A + +P+G++L  T   +GL++
Sbjct:    7 LAKKAKEASKKLAQLSTEQKNRALLKIAQYLEENMEKILTENQKDLAEAKKGGLSPAFIERLTLNEKRILDMAEGVRQVAKLPDPVGEVLGMTRRPNGLVI 107

HSP 2 Score: 29.261 bits (64), Expect = 8.939e-13
Identity = 13/35 (37.14%), Postives = 21/35 (60.00%), Query Frame = 2 
Query:  707 GETSCXLGVLLIVFESRPDALVQIASWAHSSGNGL 811
            G+    LGV+ I++ESRP+  V  A+    +GN +
Sbjct:  108 GQVRVPLGVVGIIYESRPNVTVDAAALCLKAGNAV 142
[Close]
BLAST of EY753720 vs. ExPASy Swiss-Prot
Match: PROA_PROMM (Gamma-glutamyl phosphate reductase OS=Prochlorococcus marinus (strain MIT 9313) GN=proA PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.159e-12
Identity = 36/113 (31.86%), Postives = 61/113 (53.98%), Query Frame = 3 
Query:  381 VKQVGAREMAVADEKSSRRLQALQSQD--RRKILLDVADALEANESLIKVENEADVAAAQEAGYAKSLVSRLALKPGKISSLAKSIRILADMEEPIGQILKRTELADGLILEK 713
            V +  A+ + +A E     +   QS D  RRK L+ +A+AL ++   I   N+ D+  A+  G A +L++RL L   K++S  + +R LA + +P+G      EL   L+LE+
Sbjct:    7 VPEPSAQLLRLAAEVRQAAMALGQSDDNQRRKALMAMANALLSSSEQIVRANQLDLEKARTEGLASALMARLRLDESKLNSAIEGLRQLAQLTDPLGLRQLHRELDQDLVLER 119

HSP 2 Score: 39.6614 bits (91), Expect = 1.159e-12
Identity = 18/28 (64.29%), Postives = 23/28 (82.14%), Query Frame = 2 
Query:  725 LGVLLIVFESRPDALVQIASWAHSSGNG 808
            LGVL ++FE+RPDA++QI S A  SGNG
Sbjct:  124 LGVLGVIFEARPDAVIQITSLAIRSGNG 151
[Close]
BLAST of EY753720 vs. ExPASy Swiss-Prot
Match: PROA_ANAVT (Gamma-glutamyl phosphate reductase OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937) GN=proA PE=3 SV=2)

HSP 1 Score: 52.7582 bits (125), Expect = 1.159e-12
Identity = 32/104 (30.77%), Postives = 54/104 (51.92%), Query Frame = 3 
Query:  402 EMAVADEKSSRRLQALQSQDRRKILLDVADALEANESLIKVENEADVAAAQEAGYAKSLVSRLALKPGKISSLAKSIRILADMEEPIGQILKRTELADGLILEK 713
            ++A    +++  L  L ++ + + +  VA ALE+ +  I   N AD  AA   G AK L  RL L   K+      +R +  + +PIGQ+  + EL  GL+L++
Sbjct:   12 DIAQQTRQAASLLAMLSTEAKNQAIAAVAQALESAKEEILQANIADCEAATAEGIAKPLYKRLQLDEHKLRDAIAGVRDVGKLADPIGQVQIQRELDTGLVLKR 115

HSP 2 Score: 41.5874 bits (96), Expect = 1.159e-12
Identity = 19/32 (59.38%), Postives = 25/32 (78.12%), Query Frame = 2 
Query:  716 SCXLGVLLIVFESRPDALVQIASWAHSSGNGL 811
            +C LGVL I+FE+RP+A +QI S A  SGNG+
Sbjct:  117 TCPLGVLGIIFEARPEAAIQIISLAIKSGNGV 148
[Close]
BLAST of EY753720 vs. ExPASy Swiss-Prot
Match: PROA_SALNS (Gamma-glutamyl phosphate reductase OS=Salmonella newport (strain SL254) GN=proA PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.161e-12
Identity = 33/107 (30.84%), Postives = 62/107 (57.94%), Query Frame = 3 
Query:  402 EMAVADEKSSRRLQALQSQDRRKILLDVADALEANESLIKVENEADVAAAQEAGYAKSLVSRLALKPGKISSLAKSIRILADMEEPIGQILKRTELADGLILEKHHV 722
            +M +A + +S +L  L S ++ ++L  +AD LEA    I   N  DV  A+  G +++++ RL L P ++ ++A  +R + ++ +P+GQ++    L  GL LE+  V
Sbjct:    4 QMGIAAKAASYKLALLSSGEKNRVLEKIADELEAQMESILSANVQDVEQARANGLSEAMLDRLTLTPARLKAIADDVRQVCNLADPVGQVIDGGLLDSGLRLERRRV 110

HSP 2 Score: 28.4906 bits (62), Expect = 1.161e-12
Identity = 11/29 (37.93%), Postives = 20/29 (68.97%), Query Frame = 2 
Query:  725 LGVLLIVFESRPDALVQIASWAHSSGNGL 811
            LGV+ +++E+RP+  V +AS    +GN +
Sbjct:  112 LGVVGVIYEARPNVTVDVASLCLKTGNAV 140
[Close]
BLAST of EY753720 vs. ExPASy Swiss-Prot
Match: PROA_SALTI (Gamma-glutamyl phosphate reductase OS=Salmonella typhi GN=proA PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.507e-12
Identity = 34/107 (31.78%), Postives = 62/107 (57.94%), Query Frame = 3 
Query:  402 EMAVADEKSSRRLQALQSQDRRKILLDVADALEANESLIKVENEADVAAAQEAGYAKSLVSRLALKPGKISSLAKSIRILADMEEPIGQILKRTELADGLILEKHHV 722
            +M +A + +S +L  L S ++ ++L  +AD LEA    I   N  DV  A+  G +++++ RLAL P ++ ++A  +R + ++ +P GQ++    L  GL LE+  V
Sbjct:    4 QMGIAAKAASYKLALLSSCEKNRVLEKIADELEAQMESILSANVQDVEQARANGLSEAMLDRLALTPARLKAIADDVRQVCNLADPAGQVIDGGLLDSGLRLERRRV 110

HSP 2 Score: 28.4906 bits (62), Expect = 1.507e-12
Identity = 11/29 (37.93%), Postives = 20/29 (68.97%), Query Frame = 2 
Query:  725 LGVLLIVFESRPDALVQIASWAHSSGNGL 811
            LGV+ +++E+RP+  V +AS    +GN +
Sbjct:  112 LGVVGVIYEARPNVTVDVASLCLKTGNAV 140
[Close]
BLAST of EY753720 vs. ExPASy Swiss-Prot
Match: PROA_SYNR3 (Gamma-glutamyl phosphate reductase OS=Synechococcus sp. (strain RCC307) GN=proA PE=3 SV=2)

HSP 1 Score: 54.6842 bits (130), Expect = 1.954e-12
Identity = 32/92 (34.78%), Postives = 49/92 (53.26%), Query Frame = 3 
Query:  438 LQALQSQDRRKILLDVADALEANESLIKVENEADVAAAQEAGYAKSLVSRLALKPGKISSLAKSIRILADMEEPIGQILKRTELADGLILEK 713
            L     Q R++ L  +ADALE     I + N+ D+ AA++   A +L+SRL L   K+      IR LA + +P+ Q      L +GL+LE+
Sbjct:   35 LAQCSDQQRQEALRAMADALEQQREAILLANQDDLQAAEQEQLAPALLSRLKLDRTKLDGAIAGIRQLAQLPDPLAQRQLHRALDEGLVLER 126

HSP 2 Score: 38.891 bits (89), Expect = 1.954e-12
Identity = 18/31 (58.06%), Postives = 25/31 (80.65%), Query Frame = 2 
Query:  716 SCXLGVLLIVFESRPDALVQIASWAHSSGNG 808
            S  LGV+ ++FE+RPDA++QIA+ A  SGNG
Sbjct:  128 SVPLGVVGVIFEARPDAVMQIAALAIRSGNG 158
[Close]
BLAST of EY753720 vs. ExPASy Swiss-Prot
Match: PROA_SODGM (Gamma-glutamyl phosphate reductase OS=Sodalis glossinidius (strain morsitans) GN=proA PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.957e-12
Identity = 36/107 (33.64%), Postives = 59/107 (55.14%), Query Frame = 3 
Query:  402 EMAVADEKSSRRLQALQSQDRRKILLDVADALEANESLIKVENEADVAAAQEAGYAKSLVSRLALKPGKISSLAKSIRILADMEEPIGQILKRTELADGLILEKHHV 722
            +M  A + +S +L  L S  +   L  +AD+LEAN   I   N  D+A A+  G   +L+ RL L P ++S++A  +R +  + +P+GQ++    L  GL LE+  V
Sbjct:    4 QMGKAAKAASWQLAVLTSAQKDSALQVIADSLEANSQSILDANAQDMAEARRNGLVDALLDRLLLNPARLSAIAADVRQVCRLTDPVGQVIDGQRLDSGLSLERRRV 110

HSP 2 Score: 27.335 bits (59), Expect = 1.957e-12
Identity = 10/29 (34.48%), Postives = 20/29 (68.97%), Query Frame = 2 
Query:  725 LGVLLIVFESRPDALVQIASWAHSSGNGL 811
            LGV+ +++E+RP+  V +A+    +GN +
Sbjct:  112 LGVVGVIYEARPNVTVDVAALCLKTGNAV 140
The following BLAST results are available for this feature:
BLAST of EY753720 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 147
Match NameE-valueIdentityDescription
PROA_LEPBP8.935e-1330.77Gamma-glutamyl phosphate reductase OS=Leptospira b... [more]
PROA_LEPBA8.935e-1330.77Gamma-glutamyl phosphate reductase OS=Leptospira b... [more]
PROA_PROMH8.937e-1331.78Gamma-glutamyl phosphate reductase OS=Proteus mira... [more]
PROA_CARHZ8.939e-1329.70Gamma-glutamyl phosphate reductase OS=Carboxydothe... [more]
PROA_PROMM1.159e-1231.86Gamma-glutamyl phosphate reductase OS=Prochlorococ... [more]
PROA_ANAVT1.159e-1230.77Gamma-glutamyl phosphate reductase OS=Anabaena var... [more]
PROA_SALNS1.161e-1230.84Gamma-glutamyl phosphate reductase OS=Salmonella n... [more]
PROA_SALTI1.507e-1231.78Gamma-glutamyl phosphate reductase OS=Salmonella t... [more]
PROA_SYNR31.954e-1234.78Gamma-glutamyl phosphate reductase OS=Synechococcu... [more]
PROA_SODGM1.957e-1233.64Gamma-glutamyl phosphate reductase OS=Sodalis glos... [more]

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Properties
Property NameValue
Genbank descriptionCS12-C1-001-006-B03-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY753720 ID=EY753720; Name=EY753720; organism=Citrus sinensis; type=EST; length=901bp
CTAGGAAAGCACCATACGAGGATTCTTCTGGTATATTTTGGGATAATGAC
AGTCTGGCAGGATTATTGGCTTTGGAACTAAAAGCTGATCTTCTTGTTCT
GTTGAGTGATGTAGAGGGCCTCTATAGTGGTCCTCCAAGTGAGCCAAATT
CGAAGCTGATCCATACATATATAAGAGCAAAACACCAAGCAGAAATAACA
TTTGGAGACAAGTCAAGGGTTGGAAGAGGGGGAATGACTGCTAAGGTCAA
TGCAGCTTATTGTGCAGCTTGCGCCGGTATCCCTGTTGTTATTACTAGTG
GCTTTGCCATGGACAGCATCATAAAGGTACTTGAAGGAAAGCGTGTTGGG
ACTCTCTTTCATAGAGATGCACATTTGTGGGTAAAACAAGTTGGGGCCCG
TGAGATGGCAGTTGCAGACGAAAAAAGTTCTAGACGGCTTCAGGCTCTAC
AATCTCAAGATAGGAGGAAAATTTTGCTCGACGTAGCTGATGCCTTAGAG
GCAAATGAAAGCTTGATTAAGGTTGAGAATGAAGCCGATGTAGCTGCTGC
ACAGGAAGCTGGATATGCGAAATCACTAGTATCACGTTTGGCACTGAAGC
CTGGGAAGATCTCTAGCCTTGCAAAATCTATTCGTATACTTGCAGACATG
GAAGAACCCATTGGTCAAATTTTAAAGAGAACAGAGCTTGCGGATGGACT
CATCCTGGAGAAACATCATGTNCTTTGGGCGTNCTCTTGATAGTATTTGA
ATCTCGACCTGATGCCCTGGTCCAGATAGCTTCATGGGCCCATTCGAGTG
GGAATGGTCTCTACTGAAAGGAAGAAAAGAGCTTTGCGGNCAATGCATTT
TGCACAAGTCATCACTCAGCTTCCAAATACTGTGGTGAGAACTTATTGAC
T
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