EY753425

Overview
NameEY753425
Unique NameEY753425
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length877
Libraries
Library NameType
Sweet orange leaf, field plant Acdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13099 contig Csv1_Contig13099:1..877. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_NOSP7 (Phosphoglycerate kinase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) GN=pgk PE=3 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 3.731e-22
Identity = 51/102 (50.00%), Postives = 63/102 (61.76%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCMXPXV 718
            M+K S+  LS AD+ GK+  VR D NVP+             AA+PTI+ L Q GAKVIL+SH GRPKGV  K  L P+  RLSELLG +V+K DD +   V
Sbjct:    1 MSKKSLASLSSADISGKRALVRVDFNVPVDDQGKITDDTRIRAALPTIQDLTQKGAKVILASHFGRPKGVDDKLRLTPVAKRLSELLGQEVIKTDDSIGDEV 102

HSP 2 Score: 28.1054 bits (61), Expect = 3.731e-22
Identity = 13/15 (86.67%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEK 797
            VLLL NVRFY EEEK
Sbjct:  114 VLLLENVRFYPEEEK 128
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_GLOVI (Phosphoglycerate kinase OS=Gloeobacter violaceus GN=pgk PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.808e-21
Identity = 53/106 (50.00%), Postives = 62/106 (58.49%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            MAK +V  L  ADLKGK+  VR D NVPL             AA+PTI  L   GA+VIL SHLGRPKG   +  L P+  RL ELLG  V KADD + P V++ V
Sbjct:    1 MAKKTVEALGAADLKGKRALVRVDFNVPLDESGQITDDTRIRAALPTIGMLTGGGARVILVSHLGRPKGFDDQLRLTPVAKRLGELLGQTVYKADDVIGPEVEEAV 106

HSP 2 Score: 29.6462 bits (65), Expect = 1.808e-21
Identity = 15/23 (65.22%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEKKRS*VCQK 821
            VLLL NVRFY EEEK      QK
Sbjct:  114 VLLLENVRFYPEEEKNNPEFAQK 136
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_SYNP2 (Phosphoglycerate kinase OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) GN=pgk PE=3 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.809e-21
Identity = 56/107 (52.34%), Postives = 66/107 (61.68%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            MAK SV  L+ ADL GK+VFVR D NVPL             AA+PTIK+L + GAKVIL SH+GRPKG V     L P+  RLSELLG  V K DDC+   V + +
Sbjct:    1 MAKKSVANLTEADLSGKRVFVRVDFNVPLNESGVITDDTRIRAALPTIKYLTEKGAKVILGSHMGRPKGQVVDSMRLTPVAARLSELLGQSVTKCDDCVGDAVNQAI 107

HSP 2 Score: 20.7866 bits (42), Expect = 1.809e-21
Identity = 7/9 (77.78%), Postives = 9/9 (100.00%), Query Frame = 1 
Query:  793 KKNDPEFAR 819
            +KNDPEFA+
Sbjct:  128 EKNDPEFAK 136
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_THEEB (Phosphoglycerate kinase OS=Thermosynechococcus elongatus (strain BP-1) GN=pgk PE=3 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 6.093e-21
Identity = 54/102 (52.94%), Postives = 63/102 (61.76%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCMXPXV 718
            M+K SV +LS ADL+GK+V VR D NVP+             AA+PTI+ LI  GAKVIL SH GRPKGV  K  L P+  RLSELL   V K DDC+   V
Sbjct:    1 MSKKSVAQLSAADLEGKRVLVRVDFNVPVDEKGVITDETRIRAALPTIQDLISKGAKVILVSHFGRPKGVDEKLRLTPVAQRLSELLHKPVAKLDDCIGDAV 102

HSP 2 Score: 21.557 bits (44), Expect = 6.093e-21
Identity = 10/15 (66.67%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEK 797
            V LL NVRF+  EEK
Sbjct:  114 VCLLENVRFHPGEEK 128

HSP 3 Score: 20.7866 bits (42), Expect = 6.093e-21
Identity = 7/9 (77.78%), Postives = 9/9 (100.00%), Query Frame = 1 
Query:  793 KKNDPEFAR 819
            +KNDPEFA+
Sbjct:  127 EKNDPEFAK 135
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_ANADF (Phosphoglycerate kinase OS=Anaeromyxobacter sp. (strain Fw109-5) GN=pgk PE=3 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 1.140e-20
Identity = 55/101 (54.46%), Postives = 61/101 (60.40%), Query Frame = 2 
Query:  437 LSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKGV---TPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            +   DL GK+VF+R D NVPL             AA+PTIKH IQ  AKVIL SHLGRPKG      KFSL P   RLSELL   V+ ADDC+   VKKLV
Sbjct:    6 IDALDLAGKRVFIRVDFNVPLDEQRRVTDDARIRAALPTIKHAIQARAKVILGSHLGRPKGKPDDREKFSLEPAAQRLSELLKQDVILADDCIGDGVKKLV 106

HSP 2 Score: 24.6386 bits (52), Expect = 1.140e-20
Identity = 13/20 (65.00%), Postives = 16/20 (80.00%), Query Frame = 3 
Query:  741 LKAG-VLLLGNVRFYKEEEK 797
            LK G VLLL N+RF+ +EEK
Sbjct:  109 LKEGQVLLLENLRFHPQEEK 128
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_CARHZ (Phosphoglycerate kinase OS=Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) GN=pgk PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.328e-20
Identity = 50/106 (47.17%), Postives = 70/106 (66.04%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKL 727
            M K S+ ++   ++KGKKVFVR D NVPL             AA+PT+++L + GAK+I++SHLGRPKG    K+SL P+  RL+ELLGI+V  A D + P V+K+
Sbjct:    1 MNKKSIRDV---EVKGKKVFVRVDFNVPLNENREITDDTRIRAALPTLQYLREQGAKLIVASHLGRPKGQFNEKYSLKPVAKRLAELLGIEVKMAPDVVGPEVEKM 103

HSP 2 Score: 28.1054 bits (61), Expect = 1.328e-20
Identity = 13/15 (86.67%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEK 797
            VLLL NVRFY EEEK
Sbjct:  112 VLLLENVRFYPEEEK 126

HSP 3 Score: 21.9422 bits (45), Expect = 1.328e-20
Identity = 8/9 (88.89%), Postives = 9/9 (100.00%), Query Frame = 1 
Query:  793 KKNDPEFAR 819
            +KNDPEFAR
Sbjct:  125 EKNDPEFAR 133
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_SYNSC (Phosphoglycerate kinase OS=Synechococcus sp. (strain CC9605) GN=pgk PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 1.929e-20
Identity = 53/107 (49.53%), Postives = 61/107 (57.01%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            MAK S+  L+  DL GK+V VR D NVPL             AA+PTI  LI  GAKVILS+H GRPKG V     L P+  RLSELLG  V K D C+ P  +  V
Sbjct:    1 MAKRSLASLNAGDLSGKRVLVRVDFNVPLNDTGAITDDTRIRAALPTINDLIGKGAKVILSAHFGRPKGQVNDAMRLTPVAARLSELLGKPVAKTDSCIGPDAEAKV 107

HSP 2 Score: 26.5646 bits (57), Expect = 1.929e-20
Identity = 12/23 (52.17%), Postives = 16/23 (69.57%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEKKRS*VCQK 821
            V+LL NVRF+ EEEK  +   +K
Sbjct:  115 VVLLENVRFFAEEEKNEAGFAEK 137
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_SYNPW (Phosphoglycerate kinase OS=Synechococcus sp. (strain WH7803) GN=pgk PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 1.929e-20
Identity = 53/107 (49.53%), Postives = 61/107 (57.01%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            MAK S+  L+  DL GK+V VR D NVPL             AA+PTI  LI  GAKVILS+H GRPKG V     L P+  RLSELLG  V K D C+ P  +  V
Sbjct:    1 MAKRSLASLNAGDLSGKRVLVRVDFNVPLNDAGAITDDTRIRAALPTINDLIGKGAKVILSAHFGRPKGQVNDAMRLTPVAARLSELLGKPVAKTDSCIGPDAEAKV 107

HSP 2 Score: 26.5646 bits (57), Expect = 1.929e-20
Identity = 12/23 (52.17%), Postives = 16/23 (69.57%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEKKRS*VCQK 821
            V+LL NVRF+ EEEK  +   +K
Sbjct:  115 VVLLENVRFFAEEEKNEAGFAEK 137
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_GEOLS (Phosphoglycerate kinase OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=pgk PE=3 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 2.230e-20
Identity = 48/95 (50.53%), Postives = 63/95 (66.32%), Query Frame = 2 
Query:  449 DLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            DLKGKKVF+R D NVP               AVPTIK+ ++ GAKV+L+SHLGRPKG    K+++AP   RLS+LLG +V++A DC  P V K++
Sbjct:   11 DLKGKKVFIRVDFNVPQDEKGNITEDTRIAGAVPTIKYAVEQGAKVVLASHLGRPKGEKKSKYTMAPAAKRLSQLLGKKVMQAPDCFGPDVSKII 105

HSP 2 Score: 23.483 bits (49), Expect = 2.230e-20
Identity = 10/15 (66.67%), Postives = 12/15 (80.00%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEK 797
            V++L NVRFY  EEK
Sbjct:  113 VVMLENVRFYPGEEK 127

HSP 3 Score: 20.4014 bits (41), Expect = 2.230e-20
Identity = 7/9 (77.78%), Postives = 9/9 (100.00%), Query Frame = 1 
Query:  793 KKNDPEFAR 819
            +KNDPEFA+
Sbjct:  126 EKNDPEFAQ 134
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_PROMA (Phosphoglycerate kinase OS=Prochlorococcus marinus GN=pgk PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.509e-20
Identity = 54/107 (50.47%), Postives = 65/107 (60.75%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            MAK S+  LS  DL GK+V VR D NVPL             AA+PTI+HLI+  A+VILS+H GRPKG V     L P+  RLSELLG  VVK + C+ P  +K V
Sbjct:    1 MAKRSLSNLSVEDLCGKRVLVRVDFNVPLNEEGSITDDTRIRAALPTIQHLIEKEARVILSAHFGRPKGKVNEDMRLTPVSQRLSELLGKTVVKTESCIGPDAEKKV 107

HSP 2 Score: 25.0238 bits (53), Expect = 2.509e-20
Identity = 15/30 (50.00%), Postives = 18/30 (60.00%), Query Frame = 3 
Query:  735 HXLKAG-VLLLGNVRFYKEEEKKRS*VCQK 821
            H +  G V+LL NVRF  EEEK  S   +K
Sbjct:  108 HEMSNGDVVLLENVRFIGEEEKNDSEFAKK 137
The following BLAST results are available for this feature:
BLAST of EY753425 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 328
Match NameE-valueIdentityDescription
PGK_NOSP73.731e-2250.00Phosphoglycerate kinase OS=Nostoc punctiforme (str... [more]
PGK_GLOVI1.808e-2150.00Phosphoglycerate kinase OS=Gloeobacter violaceus G... [more]
PGK_SYNP21.809e-2152.34Phosphoglycerate kinase OS=Synechococcus sp. (stra... [more]
PGK_THEEB6.093e-2152.94Phosphoglycerate kinase OS=Thermosynechococcus elo... [more]
PGK_ANADF1.140e-2054.46Phosphoglycerate kinase OS=Anaeromyxobacter sp. (s... [more]
PGK_CARHZ1.328e-2047.17Phosphoglycerate kinase OS=Carboxydothermus hydrog... [more]
PGK_SYNSC1.929e-2049.53Phosphoglycerate kinase OS=Synechococcus sp. (stra... [more]
PGK_SYNPW1.929e-2049.53Phosphoglycerate kinase OS=Synechococcus sp. (stra... [more]
PGK_GEOLS2.230e-2050.53Phosphoglycerate kinase OS=Geobacter lovleyi (stra... [more]
PGK_PROMA2.509e-2050.47Phosphoglycerate kinase OS=Prochlorococcus marinus... [more]

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Properties
Property NameValue
Genbank descriptionCS12-C1-001-002-F05-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY753425 ID=EY753425; Name=EY753425; organism=Citrus sinensis; type=EST; length=877bp
CTCTCCTTAACGATAAGAGAAATCTCCAATATCACCCAATCGTTTTTCCT
CCTCGCTCTTCACGTCCACATCTTCTTCATCTTCCCCACACATTTTCCTT
CCCCATTCTTCAATCAAACCAAAACCCACTTCCCATTTCTCCACTTTGTC
GACAAAGTTTCAATCTTTTGCGCCTTTACTACGCCACTCCACTCCCACCA
CATCAATGGCTTCAGCCACCGCTCCCACTACTTTATCTCTCGTCAAAACC
GCCGCAAGTTCATCCCTTTCTTCCCCACGCGCCTCCTTCCTGCGCATGCC
CACTTCAGCATCCCGCAGGCTGGGGTTCTCCGCAGCCGACCCGCTGCTGA
CCTGCCATGTGGCGTCGAGGCTGAGCTCGATTAAGACGAAGGGCACTCGT
GCGGTGGTGTCGATGGCGAAAAANAGCGTGGGGGAGTTGAGTGGAGCTGA
TTTGAAAGGGAAGAAGGTGTTTGTAAGAGCTGACTTGAATGTGCCTTTGG
ATGATAATCANAACATTACTGATGATACTAGAATCAGAGCTGCTGTTCCC
ACCATCAAGCATTTGATTCAAAATGGAGCTAAAGTCATTCTTTCTAGCCA
CTTGGGAAGACCAAAGGGTGTTACTCCGAAATTCAGTTTGGCTCCTCTTG
TGCCTCGTCTTTCTGAACTCCTGGGCATTCAGGTTGTGAAGGCTGATGAC
TGCATGGNTCCANNAGTAAAAAAGCTNGTANCCTCACTTNCTGAAAGCGG
GGGTTCTTCTTCTTGGAAACGTGAGGTTCTACAAGGAGGAGGAAAAAAAA
CGATCCTGAGTTTGCCAGAAGCTGGCCTCTTTGGCAGATCTCTTGTCAAT
GATGCTTTGGGACCGNTCCAGAGCCAT
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