EY753425

Overview
NameEY753425
Unique NameEY753425
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length877
Libraries
Library NameType
Sweet orange leaf, field plant Acdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13099 contig Csv1_Contig13099:1..877. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_SYNPX (Phosphoglycerate kinase OS=Synechococcus sp. (strain WH8102) GN=pgk PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 3.263e-20
Identity = 53/107 (49.53%), Postives = 61/107 (57.01%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            MAK S+  L+  DL GK+V VR D NVPL             AA+PTI  LI  GAKVILS+H GRPKG V     L P+  RLSELLG  V K D C+ P  +  V
Sbjct:    1 MAKRSLASLNAGDLSGKRVLVRVDFNVPLNEAGAITDDTRIRAALPTINDLIGKGAKVILSAHFGRPKGQVNDAMRLTPVAARLSELLGKPVAKTDSCIGPDAEAKV 107

HSP 2 Score: 25.7942 bits (55), Expect = 3.263e-20
Identity = 12/23 (52.17%), Postives = 16/23 (69.57%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEKKRS*VCQK 821
            V+LL NVRF+ EEEK  +   +K
Sbjct:  115 VVLLENVRFFAEEEKNDAGFAEK 137
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_CYAP4 (Phosphoglycerate kinase OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN=pgk PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 7.179e-20
Identity = 57/112 (50.89%), Postives = 65/112 (58.04%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG------VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            M+K +V  LS ADL GK+V VR D NVPL             AA+PTI+ L   GAKVILSSH GRPKG      V  KF L P+  RLSELLG  V K +DC+   VK  V
Sbjct:    1 MSKKTVANLSAADLAGKRVLVRVDFNVPLDDQGKITDDTRIRAALPTIQDLTSKGAKVILSSHFGRPKGETFAERVKDKFRLTPVAARLSELLGKPVPKPNDCIGEEVKAQV 112

HSP 2 Score: 21.557 bits (44), Expect = 7.179e-20
Identity = 10/14 (71.43%), Postives = 11/14 (78.57%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEE 794
            VLLL NVRF+  EE
Sbjct:  120 VLLLENVRFHPGEE 133
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_PROM3 (Phosphoglycerate kinase OS=Prochlorococcus marinus (strain MIT 9303) GN=pgk PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 9.339e-20
Identity = 49/99 (49.49%), Postives = 60/99 (60.61%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCM 706
            MAK S+  LS ADL+ K+V VR D NVPL             AA+PTI  LI  GA+VILS+H GRPKG +     L P+  RLSELLG  V+K + C+
Sbjct:    1 MAKRSLSSLSAADLRSKRVLVRVDFNVPLNENGAITDDTRIRAALPTINDLIDKGARVILSAHFGRPKGQINEGMRLTPVAKRLSELLGKTVIKTESCV 99

HSP 2 Score: 27.335 bits (59), Expect = 9.339e-20
Identity = 13/23 (56.52%), Postives = 16/23 (69.57%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEKKRS*VCQK 821
            V+LL NVRF+ EEEK  +   QK
Sbjct:  115 VVLLENVRFFSEEEKNDANFAQK 137
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_ANASK (Phosphoglycerate kinase OS=Anaeromyxobacter sp. (strain K) GN=pgk PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 9.342e-20
Identity = 52/101 (51.49%), Postives = 61/101 (60.40%), Query Frame = 2 
Query:  437 LSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKGV---TPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            +   DL GK+VF+R D NVPL             AA+PTI+H IQ  AKVIL+SHLGRPKG      K +L P   RLSELL   V+ ADDC+   VKKLV
Sbjct:    6 IDALDLAGKRVFIRVDFNVPLDPQGRVTDDARIRAALPTIRHAIQAKAKVILASHLGRPKGKPDDRQKLTLEPAAVRLSELLSQDVILADDCVGDGVKKLV 106

HSP 2 Score: 25.409 bits (54), Expect = 9.342e-20
Identity = 14/20 (70.00%), Postives = 16/20 (80.00%), Query Frame = 3 
Query:  741 LKAG-VLLLGNVRFYKEEEK 797
            LK G VLLL N+RF+ EEEK
Sbjct:  109 LKDGHVLLLENLRFHPEEEK 128
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_ANADE (Phosphoglycerate kinase OS=Anaeromyxobacter dehalogenans (strain 2CP-C) GN=pgk PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 9.342e-20
Identity = 52/101 (51.49%), Postives = 61/101 (60.40%), Query Frame = 2 
Query:  437 LSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKGV---TPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            +   DL GK+VF+R D NVPL             AA+PTI+H IQ  AKVIL+SHLGRPKG      K +L P   RLSELL   V+ ADDC+   VKKLV
Sbjct:    6 IDALDLAGKRVFIRVDFNVPLDPQGKVTDDARIRAALPTIRHAIQAKAKVILASHLGRPKGKPDDRTKLTLEPAAVRLSELLSQDVILADDCVGDGVKKLV 106

HSP 2 Score: 25.409 bits (54), Expect = 9.342e-20
Identity = 14/20 (70.00%), Postives = 16/20 (80.00%), Query Frame = 3 
Query:  741 LKAG-VLLLGNVRFYKEEEK 797
            LK G VLLL N+RF+ EEEK
Sbjct:  109 LKDGHVLLLENLRFHPEEEK 128
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_SYMTH (Phosphoglycerate kinase OS=Symbiobacterium thermophilum GN=pgk PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 9.343e-20
Identity = 51/95 (53.68%), Postives = 64/95 (67.37%), Query Frame = 2 
Query:  449 DLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKGV-TPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            D+KGK+VFVR D NVPL             AAVPTI++L++ GA  +L+SHLGRPKG    K+SLAP   RLSELLG +VV A DC+   V++LV
Sbjct:   10 DVKGKRVFVRVDFNVPLEDGRITDDTRIR-AAVPTIRYLVEEGAVAVLASHLGRPKGQRNEKYSLAPCARRLSELLGREVVFAPDCIGEEVERLV 103

HSP 2 Score: 26.9498 bits (58), Expect = 9.343e-20
Identity = 12/15 (80.00%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEK 797
            V+LL NVRFY EEEK
Sbjct:  111 VVLLENVRFYAEEEK 125
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_PORGI (Phosphoglycerate kinase OS=Porphyromonas gingivalis GN=pgk PE=3 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.214e-19
Identity = 42/83 (50.60%), Postives = 55/83 (66.27%), Query Frame = 2 
Query:  458 GKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCM 706
            GKK FVR D NVPL             AA+PT++ +I +G   I+ SHLGRPKGV P+FSL  ++P +SELLG+ V+ ADDC+
Sbjct:   10 GKKAFVRVDFNVPLDDNFKITDDTRIRAALPTLRKIIADGGMTIIGSHLGRPKGVAPEFSLRHILPHVSELLGVDVLFADDCI 92

HSP 2 Score: 28.8758 bits (63), Expect = 1.214e-19
Identity = 16/21 (76.19%), Postives = 17/21 (80.95%), Query Frame = 3 
Query:  741 LKAG-VLLLGNVRFYKEEEKK 800
            LKAG VLLL N+RFY EEE K
Sbjct:  103 LKAGQVLLLENLRFYAEEEGK 123
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_GEOSF (Phosphoglycerate kinase OS=Geobacter sp. (strain FRC-32) GN=pgk PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.215e-19
Identity = 50/95 (52.63%), Postives = 61/95 (64.21%), Query Frame = 2 
Query:  449 DLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            DL+ K VF+R D NVP               AVPTIK+ I+NGAKVIL+SHLGRPKG   PK+++AP   RLSELLG +V +A DC  P V  +V
Sbjct:   11 DLREKLVFIRVDFNVPQDDNGNITEDTRIVGAVPTIKYAIENGAKVILASHLGRPKGEKKPKYTMAPAARRLSELLGKEVKQATDCFGPDVDAMV 105

HSP 2 Score: 24.6386 bits (52), Expect = 1.215e-19
Identity = 11/15 (73.33%), Postives = 12/15 (80.00%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEK 797
            VL+L NVRFY  EEK
Sbjct:  113 VLMLENVRFYPGEEK 127
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_SYNS9 (Phosphoglycerate kinase OS=Synechococcus sp. (strain CC9902) GN=pgk PE=3 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 1.580e-19
Identity = 50/107 (46.73%), Postives = 61/107 (57.01%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            MAK S+  L+  DL GK+V VR D NVPL             AA+PTI  L+  GAKV+L++H GRPKG V     L P+  RLSELLG  V K D C+ P  +  V
Sbjct:    1 MAKRSLASLNAGDLSGKRVLVRVDFNVPLNDAGAITDDTRIRAALPTINDLVGKGAKVVLAAHFGRPKGQVNEAMRLTPVAARLSELLGKTVTKTDSCIGPDAEAKV 107

HSP 2 Score: 25.409 bits (54), Expect = 1.580e-19
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEKKRS*VCQK 821
            V+LL NVRF  EEEK  S   +K
Sbjct:  115 VVLLENVRFVAEEEKNDSGFAEK 137
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_PROMM (Phosphoglycerate kinase OS=Prochlorococcus marinus (strain MIT 9313) GN=pgk PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.580e-19
Identity = 49/99 (49.49%), Postives = 59/99 (59.60%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCM 706
            MAK S+  LS ADL+ K+V VR D NVPL             AA+PTI  LI  GA+VILS+H GRPKG +     L P+  RLSELLG  V K + C+
Sbjct:    1 MAKRSLSNLSAADLRSKRVLVRVDFNVPLNENGAITDDTRIRAALPTINDLIDKGARVILSAHFGRPKGQINEGMRLTPVAKRLSELLGKNVTKTESCV 99

HSP 2 Score: 27.335 bits (59), Expect = 1.580e-19
Identity = 13/23 (56.52%), Postives = 16/23 (69.57%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEKKRS*VCQK 821
            V+LL NVRF+ EEEK  +   QK
Sbjct:  115 VVLLENVRFFSEEEKNDANFAQK 137
The following BLAST results are available for this feature:
BLAST of EY753425 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 328
Match NameE-valueIdentityDescription
PGK_SYNPX3.263e-2049.53Phosphoglycerate kinase OS=Synechococcus sp. (stra... [more]
PGK_CYAP47.179e-2050.89Phosphoglycerate kinase OS=Cyanothece sp. (strain ... [more]
PGK_PROM39.339e-2049.49Phosphoglycerate kinase OS=Prochlorococcus marinus... [more]
PGK_ANASK9.342e-2051.49Phosphoglycerate kinase OS=Anaeromyxobacter sp. (s... [more]
PGK_ANADE9.342e-2051.49Phosphoglycerate kinase OS=Anaeromyxobacter dehalo... [more]
PGK_SYMTH9.343e-2053.68Phosphoglycerate kinase OS=Symbiobacterium thermop... [more]
PGK_PORGI1.214e-1950.60Phosphoglycerate kinase OS=Porphyromonas gingivali... [more]
PGK_GEOSF1.215e-1952.63Phosphoglycerate kinase OS=Geobacter sp. (strain F... [more]
PGK_SYNS91.580e-1946.73Phosphoglycerate kinase OS=Synechococcus sp. (stra... [more]
PGK_PROMM1.580e-1949.49Phosphoglycerate kinase OS=Prochlorococcus marinus... [more]

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Properties
Property NameValue
Genbank descriptionCS12-C1-001-002-F05-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY753425 ID=EY753425; Name=EY753425; organism=Citrus sinensis; type=EST; length=877bp
CTCTCCTTAACGATAAGAGAAATCTCCAATATCACCCAATCGTTTTTCCT
CCTCGCTCTTCACGTCCACATCTTCTTCATCTTCCCCACACATTTTCCTT
CCCCATTCTTCAATCAAACCAAAACCCACTTCCCATTTCTCCACTTTGTC
GACAAAGTTTCAATCTTTTGCGCCTTTACTACGCCACTCCACTCCCACCA
CATCAATGGCTTCAGCCACCGCTCCCACTACTTTATCTCTCGTCAAAACC
GCCGCAAGTTCATCCCTTTCTTCCCCACGCGCCTCCTTCCTGCGCATGCC
CACTTCAGCATCCCGCAGGCTGGGGTTCTCCGCAGCCGACCCGCTGCTGA
CCTGCCATGTGGCGTCGAGGCTGAGCTCGATTAAGACGAAGGGCACTCGT
GCGGTGGTGTCGATGGCGAAAAANAGCGTGGGGGAGTTGAGTGGAGCTGA
TTTGAAAGGGAAGAAGGTGTTTGTAAGAGCTGACTTGAATGTGCCTTTGG
ATGATAATCANAACATTACTGATGATACTAGAATCAGAGCTGCTGTTCCC
ACCATCAAGCATTTGATTCAAAATGGAGCTAAAGTCATTCTTTCTAGCCA
CTTGGGAAGACCAAAGGGTGTTACTCCGAAATTCAGTTTGGCTCCTCTTG
TGCCTCGTCTTTCTGAACTCCTGGGCATTCAGGTTGTGAAGGCTGATGAC
TGCATGGNTCCANNAGTAAAAAAGCTNGTANCCTCACTTNCTGAAAGCGG
GGGTTCTTCTTCTTGGAAACGTGAGGTTCTACAAGGAGGAGGAAAAAAAA
CGATCCTGAGTTTGCCAGAAGCTGGCCTCTTTGGCAGATCTCTTGTCAAT
GATGCTTTGGGACCGNTCCAGAGCCAT
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