EY753425

Overview
NameEY753425
Unique NameEY753425
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length877
Libraries
Library NameType
Sweet orange leaf, field plant Acdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13099 contig Csv1_Contig13099:1..877. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_DICT6 (Phosphoglycerate kinase OS=Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) GN=pgk PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 2.055e-19
Identity = 51/104 (49.04%), Postives = 63/104 (60.58%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKK 724
            MAK ++ +L   +LKGK+V VR D NVP+             A +PTIK+LI  GAKVIL SHLGRPKG      L P+  RLSELLG  V K +DC+   V+K
Sbjct:    1 MAKKTILDLQDEELKGKRVLVRVDFNVPIKNGVITDDRRIREA-LPTIKYLIDRGAKVILVSHLGRPKGFQDDLRLDPVAKRLSELLGKPVKKLNDCIGEEVEK 103

HSP 2 Score: 26.9498 bits (58), Expect = 2.055e-19
Identity = 11/14 (78.57%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEE 794
            V+LL N+RFYKEEE
Sbjct:  113 VVLLENIRFYKEEE 126
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_DICTD (Phosphoglycerate kinase OS=Dictyoglomus turgidum (strain Z-1310 / DSM 6724) GN=pgk PE=3 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 2.055e-19
Identity = 51/104 (49.04%), Postives = 63/104 (60.58%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKK 724
            MAK ++ +L   DLKGK+V VR D NVPL             A +PTIK+L+  GAKVIL SHLGRPKG      L P+  RLSELLG  V K +DC+   V++
Sbjct:    1 MAKKTILDLRDEDLKGKRVLVRVDFNVPLKNGVITDDRRIREA-LPTIKYLMDKGAKVILVSHLGRPKGFQDDLRLDPVAKRLSELLGKPVKKLNDCIGEEVER 103

HSP 2 Score: 27.335 bits (59), Expect = 2.055e-19
Identity = 11/14 (78.57%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEE 794
            V+LL N+RFYKEEE
Sbjct:  113 VILLENIRFYKEEE 126
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_SYNS3 (Phosphoglycerate kinase OS=Synechococcus sp. (strain CC9311) GN=pgk PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 2.672e-19
Identity = 51/107 (47.66%), Postives = 63/107 (58.88%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            MAK S+ +L+ +DL GK+V VR D NVPL             AA+PTI  LI  GAKVIL++H GRPKG V  K  L P+  RLSELL   V K + C+ P  +  V
Sbjct:    1 MAKRSLAKLNTSDLSGKRVLVRVDFNVPLNDAGAITDDTRIRAALPTINDLIGKGAKVILAAHFGRPKGQVNDKMRLTPVATRLSELLSKPVTKTESCIGPDAEAKV 107

HSP 2 Score: 25.409 bits (54), Expect = 2.672e-19
Identity = 11/15 (73.33%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEK 797
            V+LL NVRF+ EEEK
Sbjct:  115 VVLLENVRFFAEEEK 129
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_PROM5 (Phosphoglycerate kinase OS=Prochlorococcus marinus (strain MIT 9515) GN=pgk PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.672e-19
Identity = 47/99 (47.47%), Postives = 61/99 (61.62%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCM 706
            M+K S+  L  + L+GKKV VR D NVPL             AA+PTIK+LI N AKVIL++H GRPKG V  +  L P+  RLSE+LG +V   + C+
Sbjct:    1 MSKLSLSSLDKSKLEGKKVLVRVDFNVPLNDDGQITDDTRIRAAIPTIKYLINNSAKVILAAHFGRPKGKVNERMRLTPIAARLSEILGKKVNLTESCI 99

HSP 2 Score: 28.8758 bits (63), Expect = 2.672e-19
Identity = 13/15 (86.67%), Postives = 14/15 (93.33%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEK 797
            VLLL NVRFY+EEEK
Sbjct:  115 VLLLENVRFYEEEEK 129
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_ANAD2 (Phosphoglycerate kinase OS=Anaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258) GN=pgk PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 2.673e-19
Identity = 51/101 (50.50%), Postives = 61/101 (60.40%), Query Frame = 2 
Query:  437 LSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKGV---TPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            +   +L GK+VF+R D NVPL             AA+PTI+H IQ  AKVIL+SHLGRPKG      K +L P   RLSELL   V+ ADDC+   VKKLV
Sbjct:    6 IDALELAGKRVFIRVDFNVPLDPQGRVTDDARIRAALPTIRHAIQAKAKVILASHLGRPKGKPEDRQKLTLEPAAVRLSELLSQDVILADDCVGDGVKKLV 106

HSP 2 Score: 25.409 bits (54), Expect = 2.673e-19
Identity = 14/20 (70.00%), Postives = 16/20 (80.00%), Query Frame = 3 
Query:  741 LKAG-VLLLGNVRFYKEEEK 797
            LK G VLLL N+RF+ EEEK
Sbjct:  109 LKDGHVLLLENLRFHPEEEK 128
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_TRIRE (Phosphoglycerate kinase OS=Trichoderma reesei GN=pgk1 PE=3 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.472e-19
Identity = 50/100 (50.00%), Postives = 62/100 (62.00%), Query Frame = 2 
Query:  437 LSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAK-VILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            ++  DLKGK+V +R D NVPL              A+PTIKH + NGAK V+L SHLGRP G V PK+SL P+VP L  LLG  V  A DC+ P V+ +V
Sbjct:    9 ITDVDLKGKRVLIRVDFNVPLDENKKITNNQRIVGAIPTIKHALDNGAKAVVLMSHLGRPNGAVNPKYSLKPVVPELERLLGKPVTFARDCVGPEVESIV 108

HSP 2 Score: 21.557 bits (44), Expect = 3.472e-19
Identity = 9/14 (64.29%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEE 794
            V+LL N+RF+ EEE
Sbjct:  116 VILLENLRFHIEEE 129
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_SYNR3 (Phosphoglycerate kinase OS=Synechococcus sp. (strain RCC307) GN=pgk PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 3.474e-19
Identity = 50/99 (50.51%), Postives = 58/99 (58.59%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCM 706
            MAK S+  L+   L+GK+V VR D NVPL             AA+PTIK L   GAKVILS+H GRPKG V     L P+  RLSELLG  V K D C+
Sbjct:    1 MAKRSLSALNSDALRGKRVLVRVDFNVPLNDAGAITDDTRIRAALPTIKDLTGKGAKVILSAHFGRPKGQVNEDMRLTPVAARLSELLGAPVAKTDSCI 99

HSP 2 Score: 27.7202 bits (60), Expect = 3.474e-19
Identity = 13/23 (56.52%), Postives = 16/23 (69.57%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEKKRS*VCQK 821
            V+LL NVRF+ EEEK  S   +K
Sbjct:  115 VVLLENVRFFAEEEKNESGFAEK 137
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_ACAM1 (Phosphoglycerate kinase OS=Acaryochloris marina (strain MBIC 11017) GN=pgk PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 5.478e-19
Identity = 52/103 (50.49%), Postives = 62/103 (60.19%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXV 718
            M+K ++  L+ ADL GK+V VRAD NVPL             AA+PTI+ L   GAKVIL+SH GRPKG V     L P+  RLSELLG  V K DDC+   V
Sbjct:    1 MSKKTIANLTSADLSGKRVLVRADFNVPLDGDGKITDDTRIRAALPTIQDLTSKGAKVILASHFGRPKGQVNESMRLTPVAARLSELLGQAVTKCDDCIGDEV 103
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_TRIVI (Phosphoglycerate kinase OS=Trichoderma viride GN=pgk1 PE=3 SV=2)

HSP 1 Score: 93.5893 bits (231), Expect = 7.634e-19
Identity = 50/100 (50.00%), Postives = 63/100 (63.00%), Query Frame = 2 
Query:  437 LSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAK-VILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            ++  D+KGK+V +R D NVPL              A+PTIKH + NGAK VIL SHLGRP G V  K+SL P+VP+L ELLG  V  A DC+ P V+ +V
Sbjct:    9 ITDVDVKGKRVLIRVDFNVPLDENKNITNPQRIAGAIPTIKHALDNGAKAVILMSHLGRPNGAVNAKYSLKPVVPKLEELLGKPVTFAPDCVGPEVEAIV 108

HSP 2 Score: 21.557 bits (44), Expect = 7.634e-19
Identity = 9/14 (64.29%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEE 794
            V+LL N+RF+ EEE
Sbjct:  116 VILLENLRFHIEEE 129
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_GEOMG (Phosphoglycerate kinase OS=Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210) GN=pgk PE=3 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 7.642e-19
Identity = 48/94 (51.06%), Postives = 58/94 (61.70%), Query Frame = 2 
Query:  452 LKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKGV-TPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            LKGKKVF+R D NVPL             A +PTI   +  GAKVIL+SHLGRPKG   PK+S+AP   RLS LL  +V  A DC+   VKK++
Sbjct:   12 LKGKKVFIRVDFNVPLDEHQNITEDTRIRAVLPTINFALDAGAKVILASHLGRPKGERKPKYSMAPAAKRLSRLLNKEVQLAPDCIGDEVKKMI 105

HSP 2 Score: 26.1794 bits (56), Expect = 7.642e-19
Identity = 12/15 (80.00%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEK 797
            VLLL NVRFY+ EEK
Sbjct:  113 VLLLENVRFYEGEEK 127
The following BLAST results are available for this feature:
BLAST of EY753425 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 328
Match NameE-valueIdentityDescription
PGK_DICT62.055e-1949.04Phosphoglycerate kinase OS=Dictyoglomus thermophil... [more]
PGK_DICTD2.055e-1949.04Phosphoglycerate kinase OS=Dictyoglomus turgidum (... [more]
PGK_SYNS32.672e-1947.66Phosphoglycerate kinase OS=Synechococcus sp. (stra... [more]
PGK_PROM52.672e-1947.47Phosphoglycerate kinase OS=Prochlorococcus marinus... [more]
PGK_ANAD22.673e-1950.50Phosphoglycerate kinase OS=Anaeromyxobacter dehalo... [more]
PGK_TRIRE3.472e-1950.00Phosphoglycerate kinase OS=Trichoderma reesei GN=p... [more]
PGK_SYNR33.474e-1950.51Phosphoglycerate kinase OS=Synechococcus sp. (stra... [more]
PGK_ACAM15.478e-1950.49Phosphoglycerate kinase OS=Acaryochloris marina (s... [more]
PGK_TRIVI7.634e-1950.00Phosphoglycerate kinase OS=Trichoderma viride GN=p... [more]
PGK_GEOMG7.642e-1951.06Phosphoglycerate kinase OS=Geobacter metallireduce... [more]

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Properties
Property NameValue
Genbank descriptionCS12-C1-001-002-F05-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY753425 ID=EY753425; Name=EY753425; organism=Citrus sinensis; type=EST; length=877bp
CTCTCCTTAACGATAAGAGAAATCTCCAATATCACCCAATCGTTTTTCCT
CCTCGCTCTTCACGTCCACATCTTCTTCATCTTCCCCACACATTTTCCTT
CCCCATTCTTCAATCAAACCAAAACCCACTTCCCATTTCTCCACTTTGTC
GACAAAGTTTCAATCTTTTGCGCCTTTACTACGCCACTCCACTCCCACCA
CATCAATGGCTTCAGCCACCGCTCCCACTACTTTATCTCTCGTCAAAACC
GCCGCAAGTTCATCCCTTTCTTCCCCACGCGCCTCCTTCCTGCGCATGCC
CACTTCAGCATCCCGCAGGCTGGGGTTCTCCGCAGCCGACCCGCTGCTGA
CCTGCCATGTGGCGTCGAGGCTGAGCTCGATTAAGACGAAGGGCACTCGT
GCGGTGGTGTCGATGGCGAAAAANAGCGTGGGGGAGTTGAGTGGAGCTGA
TTTGAAAGGGAAGAAGGTGTTTGTAAGAGCTGACTTGAATGTGCCTTTGG
ATGATAATCANAACATTACTGATGATACTAGAATCAGAGCTGCTGTTCCC
ACCATCAAGCATTTGATTCAAAATGGAGCTAAAGTCATTCTTTCTAGCCA
CTTGGGAAGACCAAAGGGTGTTACTCCGAAATTCAGTTTGGCTCCTCTTG
TGCCTCGTCTTTCTGAACTCCTGGGCATTCAGGTTGTGAAGGCTGATGAC
TGCATGGNTCCANNAGTAAAAAAGCTNGTANCCTCACTTNCTGAAAGCGG
GGGTTCTTCTTCTTGGAAACGTGAGGTTCTACAAGGAGGAGGAAAAAAAA
CGATCCTGAGTTTGCCAGAAGCTGGCCTCTTTGGCAGATCTCTTGTCAAT
GATGCTTTGGGACCGNTCCAGAGCCAT
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