EY753425

Overview
NameEY753425
Unique NameEY753425
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length877
Libraries
Library NameType
Sweet orange leaf, field plant Acdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13099 contig Csv1_Contig13099:1..877. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_PROM2 (Phosphoglycerate kinase OS=Prochlorococcus marinus (strain MIT 9215) GN=pgk PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 6.244e-18
Identity = 47/99 (47.47%), Postives = 59/99 (59.60%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCM 706
            M+K S+  L    L+GKKV VR D NVPL             AA+PTI++LI + AKVIL++H GRPKG V  K  L P+  RLSELLG  V   + C+
Sbjct:    1 MSKLSLSSLDKTHLEGKKVLVRVDFNVPLNEDGQITDDTRIRAAIPTIEYLINHSAKVILAAHFGRPKGQVNEKMRLTPVAARLSELLGQNVALTNSCI 99

HSP 2 Score: 26.1794 bits (56), Expect = 6.244e-18
Identity = 12/15 (80.00%), Postives = 13/15 (86.67%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEK 797
            VLLL NVRF+ EEEK
Sbjct:  115 VLLLENVRFFGEEEK 129
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_PROM0 (Phosphoglycerate kinase OS=Prochlorococcus marinus (strain MIT 9301) GN=pgk PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 6.244e-18
Identity = 46/99 (46.46%), Postives = 59/99 (59.60%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCM 706
            M+K S+  L    L+GKKV VR D NVPL             AA+PTI++L+ + AKVIL++H GRPKG V  K  L P+  RLSELLG  V   + C+
Sbjct:    1 MSKLSLSSLDKTHLEGKKVLVRVDFNVPLNEDGQITDDTRIRAAIPTIEYLVNHSAKVILAAHFGRPKGQVNEKMRLTPVAARLSELLGQSVALTNSCI 99

HSP 2 Score: 26.5646 bits (57), Expect = 6.244e-18
Identity = 14/23 (60.87%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEKKRS*VCQK 821
            VLLL NVRF+ EEEK      QK
Sbjct:  115 VLLLENVRFFGEEEKNDLEFAQK 137
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_CYAP8 (Phosphoglycerate kinase OS=Cyanothece sp. (strain PCC 8801) GN=pgk PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 6.245e-18
Identity = 54/103 (52.43%), Postives = 61/103 (59.22%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXV 718
            M K S+  LS AD+ GK+V VR D NVPL             AA+PTIK LI+ GAKVIL SH GRPKG V     L P+  RLSELLG  VV  DDC+   V
Sbjct:    1 MTKKSIVNLSPADVAGKRVLVRVDFNVPLDGATITDDTRIR-AALPTIKDLIEKGAKVILCSHFGRPKGQVVESMRLTPVATRLSELLGQPVVMCDDCIGDSV 102

HSP 2 Score: 21.557 bits (44), Expect = 6.245e-18
Identity = 11/23 (47.83%), Postives = 13/23 (56.52%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEEKKRS*VCQK 821
            V LL N+RF+ EEE       QK
Sbjct:  114 VALLENLRFHAEEEANDPEFAQK 136
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_PELPD (Phosphoglycerate kinase OS=Pelobacter propionicus (strain DSM 2379) GN=pgk PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 8.120e-18
Identity = 46/94 (48.94%), Postives = 58/94 (61.70%), Query Frame = 2 
Query:  446 ADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKK 724
            ADLK KKVF+R D NVP               A+PTI++ I+ GA+VIL+SHLGRPKG    K+S+ P   RLSELLG +V +A DC    V +
Sbjct:   10 ADLKDKKVFIRVDFNVPQDSKGNITEDTRITGALPTIRYAIEQGARVILASHLGRPKGEKNEKYSMVPAAKRLSELLGRKVKQASDCFGEEVTR 103

HSP 2 Score: 25.409 bits (54), Expect = 8.120e-18
Identity = 13/20 (65.00%), Postives = 16/20 (80.00%), Query Frame = 3 
Query:  741 LKAG-VLLLGNVRFYKEEEK 797
            +K+G VL+L NVRFY  EEK
Sbjct:  108 MKSGEVLMLENVRFYPGEEK 127
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_PENCH (Phosphoglycerate kinase OS=Penicillium chrysogenum GN=PGKA PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.371e-17
Identity = 48/100 (48.00%), Postives = 61/100 (61.00%), Query Frame = 2 
Query:  437 LSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAK-VILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            ++  DLKGK+V +R D NVPL              A+PTIK+ I NG K V+L SHLGRP G V PK+SL P+VP L ELLG  V   +DC+ P  ++ V
Sbjct:    9 VTDVDLKGKRVLIRVDFNVPLDENENVTNPQRIVGALPTIKYAIDNGRKAVVLMSHLGRPDGKVNPKYSLKPVVPVLEELLGKSVTFTEDCIGPQTEETV 108

HSP 2 Score: 22.3274 bits (46), Expect = 1.371e-17
Identity = 9/14 (64.29%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEE 794
            V+LL N+RF+ EEE
Sbjct:  116 VILLENLRFHAEEE 129
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_SYNJB (Phosphoglycerate kinase OS=Synechococcus sp. GN=pgk PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.783e-17
Identity = 55/118 (46.61%), Postives = 67/118 (56.78%), Query Frame = 2 
Query:  413 MAKXSVGEL--SGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRP----------KGVTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            MAK ++G L  SG DL GK+V VRAD NVPL             AA+PT++ L Q GAKVIL+SHLGRP          K      SLAP+  RLS+LLG  V  A DC+ P  + +V
Sbjct:    1 MAKQTLGSLLSSGFDLAGKRVLVRADFNVPLDAQGNITDDTRIRAALPTVQALTQAGAKVILTSHLGRPVKKDKETGAVKVAREGNSLAPVATRLSQLLGQPVAFAPDCIGPEAEAVV 118

HSP 2 Score: 24.2534 bits (51), Expect = 1.783e-17
Identity = 11/14 (78.57%), Postives = 11/14 (78.57%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEE 794
            V LL NVRFY EEE
Sbjct:  126 VALLENVRFYPEEE 139
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_SYNP6 (Phosphoglycerate kinase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=pgk PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 2.319e-17
Identity = 48/99 (48.48%), Postives = 61/99 (61.62%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCM 706
            M+K ++  L+ ADL+GK+V VR D NVPL             AA+PTI++L ++GAKVIL SH GRPKG       L P+  RLSELLG  VVK  D +
Sbjct:    1 MSKRTLASLTAADLEGKRVLVRVDFNVPLDGNGKITDDTRIRAALPTIRYLSESGAKVILVSHFGRPKGKPVESMRLTPVAERLSELLGRPVVKTTDAV 99

HSP 2 Score: 23.0978 bits (48), Expect = 2.319e-17
Identity = 10/14 (71.43%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEE 794
            V+LL NVRF+ EEE
Sbjct:  115 VVLLENVRFHAEEE 128
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_SYNE7 (Phosphoglycerate kinase OS=Synechococcus elongatus (strain PCC 7942) GN=pgk PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 2.319e-17
Identity = 48/99 (48.48%), Postives = 61/99 (61.62%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCM 706
            M+K ++  L+ ADL+GK+V VR D NVPL             AA+PTI++L ++GAKVIL SH GRPKG       L P+  RLSELLG  VVK  D +
Sbjct:    1 MSKRTLASLTAADLEGKRVLVRVDFNVPLDGNGKITDDTRIRAALPTIRYLSESGAKVILVSHFGRPKGKPVESMRLTPVAERLSELLGRPVVKTTDAV 99

HSP 2 Score: 23.0978 bits (48), Expect = 2.319e-17
Identity = 10/14 (71.43%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEE 794
            V+LL NVRF+ EEE
Sbjct:  115 VVLLENVRFHAEEE 128
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_AQUAE (Phosphoglycerate kinase OS=Aquifex aeolicus GN=pgk PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.320e-17
Identity = 45/98 (45.92%), Postives = 60/98 (61.22%), Query Frame = 2 
Query:  437 LSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            L   D+KGK+V VR D NVPL             A++PT+++L+   AKVIL SHLGRPK   PK+SLAP+  RLS  +  +V  A DC+   VK++V
Sbjct:    7 LRDVDVKGKRVLVRVDYNVPLDEQGNIVDDTRIRASLPTVEYLLDANAKVILMSHLGRPKNRDPKYSLAPVAKRLSRYINKEVKLAPDCVGEEVKRIV 104

HSP 2 Score: 22.3274 bits (46), Expect = 2.320e-17
Identity = 9/13 (69.23%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEE 791
            V+LL N+RF+KEE
Sbjct:  112 VVLLENLRFHKEE 124
[Close]
BLAST of EY753425 vs. ExPASy Swiss-Prot
Match: PGK_TRIEI (Phosphoglycerate kinase OS=Trichodesmium erythraeum (strain IMS101) GN=pgk PE=3 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 3.921e-17
Identity = 52/107 (48.60%), Postives = 64/107 (59.81%), Query Frame = 2 
Query:  413 MAKXSVGELSGADLKGKKVFVRADLNVPLXXXXXXXXXXXXXAAVPTIKHLIQNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELLGIQVVKADDCMXPXVKKLV 730
            M K +V  LS +DL GKKV +RAD NVP+             AA+PTI+ L + GAKVIL+SH GRPKG V  K  L  +  RLSE+LG +V K DDC+   V   V
Sbjct:    1 MTKKTVASLSKSDLSGKKVLMRADFNVPVDNGSITDDTRIR-AALPTIQDLTEKGAKVILTSHFGRPKGKVNEKMRLTLVGERLSEVLGKEVKKCDDCIGDEVTSTV 106

HSP 2 Score: 22.7126 bits (47), Expect = 3.921e-17
Identity = 10/14 (71.43%), Postives = 11/14 (78.57%), Query Frame = 3 
Query:  753 VLLLGNVRFYKEEE 794
            V+LL NVRFY  EE
Sbjct:  114 VVLLENVRFYSGEE 127
The following BLAST results are available for this feature:
BLAST of EY753425 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 328
Match NameE-valueIdentityDescription
PGK_PROM26.244e-1847.47Phosphoglycerate kinase OS=Prochlorococcus marinus... [more]
PGK_PROM06.244e-1846.46Phosphoglycerate kinase OS=Prochlorococcus marinus... [more]
PGK_CYAP86.245e-1852.43Phosphoglycerate kinase OS=Cyanothece sp. (strain ... [more]
PGK_PELPD8.120e-1848.94Phosphoglycerate kinase OS=Pelobacter propionicus ... [more]
PGK_PENCH1.371e-1748.00Phosphoglycerate kinase OS=Penicillium chrysogenum... [more]
PGK_SYNJB1.783e-1746.61Phosphoglycerate kinase OS=Synechococcus sp. GN=pg... [more]
PGK_SYNP62.319e-1748.48Phosphoglycerate kinase OS=Synechococcus sp. (stra... [more]
PGK_SYNE72.319e-1748.48Phosphoglycerate kinase OS=Synechococcus elongatus... [more]
PGK_AQUAE2.320e-1745.92Phosphoglycerate kinase OS=Aquifex aeolicus GN=pgk... [more]
PGK_TRIEI3.921e-1748.60Phosphoglycerate kinase OS=Trichodesmium erythraeu... [more]

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Properties
Property NameValue
Genbank descriptionCS12-C1-001-002-F05-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY753425 ID=EY753425; Name=EY753425; organism=Citrus sinensis; type=EST; length=877bp
CTCTCCTTAACGATAAGAGAAATCTCCAATATCACCCAATCGTTTTTCCT
CCTCGCTCTTCACGTCCACATCTTCTTCATCTTCCCCACACATTTTCCTT
CCCCATTCTTCAATCAAACCAAAACCCACTTCCCATTTCTCCACTTTGTC
GACAAAGTTTCAATCTTTTGCGCCTTTACTACGCCACTCCACTCCCACCA
CATCAATGGCTTCAGCCACCGCTCCCACTACTTTATCTCTCGTCAAAACC
GCCGCAAGTTCATCCCTTTCTTCCCCACGCGCCTCCTTCCTGCGCATGCC
CACTTCAGCATCCCGCAGGCTGGGGTTCTCCGCAGCCGACCCGCTGCTGA
CCTGCCATGTGGCGTCGAGGCTGAGCTCGATTAAGACGAAGGGCACTCGT
GCGGTGGTGTCGATGGCGAAAAANAGCGTGGGGGAGTTGAGTGGAGCTGA
TTTGAAAGGGAAGAAGGTGTTTGTAAGAGCTGACTTGAATGTGCCTTTGG
ATGATAATCANAACATTACTGATGATACTAGAATCAGAGCTGCTGTTCCC
ACCATCAAGCATTTGATTCAAAATGGAGCTAAAGTCATTCTTTCTAGCCA
CTTGGGAAGACCAAAGGGTGTTACTCCGAAATTCAGTTTGGCTCCTCTTG
TGCCTCGTCTTTCTGAACTCCTGGGCATTCAGGTTGTGAAGGCTGATGAC
TGCATGGNTCCANNAGTAAAAAAGCTNGTANCCTCACTTNCTGAAAGCGG
GGGTTCTTCTTCTTGGAAACGTGAGGTTCTACAAGGAGGAGGAAAAAAAA
CGATCCTGAGTTTGCCAGAAGCTGGCCTCTTTGGCAGATCTCTTGTCAAT
GATGCTTTGGGACCGNTCCAGAGCCAT
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