EY753420

Overview
NameEY753420
Unique NameEY753420
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length896
Libraries
Library NameType
Sweet orange leaf, field plant Acdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9230 contig Csv1_Contig9230:13..907. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY753420 vs. ExPASy Swiss-Prot
Match: CB2_PHYPA (Chlorophyll a-b binding protein, chloroplastic OS=Physcomitrella patens PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 2.092e-23
Identity = 66/202 (32.67%), Postives = 95/202 (47.03%), Query Frame = 2 
Query:  137 ETARQILGARSAQSPTGSSRKGSFVVRAASTPPVKQGADRPLWFA--SKQSLSYLDGSLPGDYGFDPLGLS-DPEGTGGFIEPKWLAYGEVINGRYAMLGAVGAIAPEILGKAGLIPQETALAWFQTGV-IPPAGTYNYWADP--------YTLFVLEMALMGFAEHRRFQDWANPGSMGRQYFLGFEKYLGGSGDPAYPGG 706
            E +R++LG   A+     +     V ++A +  +  GADRP +    S ++ SYL+G   GDYG+D  GLS DPE    F   + L   EVI+ R+AMLGA+G + PE+L K+G+   E    WF+ G  I   G  +Y  +P          ++  ++ LMG  E  R                     LG   DP YPGG
Sbjct:   23 ELSRKVLGNVEARVTMRRT-----VSKSAGSDTIWYGADRPKFLGPFSGETPSYLNGEFAGDYGWDTAGLSSDPET---FARNREL---EVIHARWAMLGALGCLTPELLAKSGVKFGEA--VWFKAGAQIFSEGGLDYLGNPSLVHAQSILAIWACQVVLMGAVEGYRVAGGP----------------LGEVTDPIYPGG 195

HSP 2 Score: 56.9954 bits (136), Expect = 2.092e-23
Identity = 26/53 (49.06%), Postives = 35/53 (66.04%), Query Frame = 1 
Query:  709 SIQPLGLGKDEKSRKDFKLKEVKNGRXXMXAILGYFVQGLVTRSGALQNLWDH 867
            S  PLGL  D  +  + K+KE+KNGR  M ++ G+FVQ +VT  G L+NL DH
Sbjct:  196 SFDPLGLADDPDTFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPLENLNDH 248
[Close]
BLAST of EY753420 vs. ExPASy Swiss-Prot
Match: CB25_SOLLC (Chlorophyll a-b binding protein 5, chloroplastic (Fragment) OS=Solanum lycopersicum GN=CAB5 PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.731e-23
Identity = 63/162 (38.89%), Postives = 83/162 (51.23%), Query Frame = 2 
Query:  245 GADRPLWFA--SKQSLSYLDGSLPGDYGFDPLGLS-DPEGTGGFIEPKWLAYGEVINGRYAMLGAVGAIAPEILGKAGLIPQETALAWFQTG-VIPPAGTYNYWADP--------YTLFVLEMALMGFAEHRRFQDWANPGSMGRQYFLGFEK-YLGGSGDP 691
            G DRP +    S+Q+ SYL G  PGDYG+D  GLS DPE    F   + L   EVI+ R+AMLGA+G + PEIL K G+   E    WF+ G  I   G  +Y  +P          ++  ++ LMGF E  R       G +G     G +K Y GG+ DP
Sbjct:   23 GEDRPKYLGPFSEQTPSYLTGEFPGDYGWDTAGLSADPE---TFARNREL---EVIHCRWAMLGALGCVFPEILSKNGVTFGEA--VWFKAGSQIFSEGGLDYLGNPNLIHAQSILAIWASQVVLMGFVEGYR----VGGGPLGE----GLDKIYPGGAFDP 168

HSP 2 Score: 56.225 bits (134), Expect = 2.731e-23
Identity = 24/53 (45.28%), Postives = 36/53 (67.92%), Query Frame = 1 
Query:  709 SIQPLGLGKDEKSRKDFKLKEVKNGRXXMXAILGYFVQGLVTRSGALQNLWDH 867
            +  PLGL  D ++  + K+KE+KNGR  M ++ G+FVQ +VT  G ++NL DH
Sbjct:  165 AFDPLGLADDPEAFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPIENLSDH 217
[Close]
BLAST of EY753420 vs. ExPASy Swiss-Prot
Match: CB24_SOLLC (Chlorophyll a-b binding protein 4, chloroplastic OS=Solanum lycopersicum GN=CAB4 PE=2 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 3.544e-23
Identity = 63/162 (38.89%), Postives = 83/162 (51.23%), Query Frame = 2 
Query:  245 GADRPLWFA--SKQSLSYLDGSLPGDYGFDPLGLS-DPEGTGGFIEPKWLAYGEVINGRYAMLGAVGAIAPEILGKAGLIPQETALAWFQTG-VIPPAGTYNYWADP--------YTLFVLEMALMGFAEHRRFQDWANPGSMGRQYFLGFEK-YLGGSGDP 691
            G DRP +    S+Q+ SYL G  PGDYG+D  GLS DPE    F   + L   EVI+ R+AMLGA+G + PEIL K G+   E    WF+ G  I   G  +Y  +P          ++  ++ LMGF E  R       G +G     G +K Y GG+ DP
Sbjct:   51 GEDRPKYLGPFSEQTPSYLTGEFPGDYGWDTAGLSADPE---TFARNREL---EVIHCRWAMLGALGCVFPEILSKNGVKFGEA--VWFKAGSQIFSEGGLDYLGNPNLVHAQSILAIWACQVVLMGFVEGYR----VGGGPLGE----GLDKIYPGGAFDP 196

HSP 2 Score: 56.225 bits (134), Expect = 3.544e-23
Identity = 24/53 (45.28%), Postives = 36/53 (67.92%), Query Frame = 1 
Query:  709 SIQPLGLGKDEKSRKDFKLKEVKNGRXXMXAILGYFVQGLVTRSGALQNLWDH 867
            +  PLGL  D ++  + K+KE+KNGR  M ++ G+FVQ +VT  G ++NL DH
Sbjct:  193 AFDPLGLADDPEAFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPIENLSDH 245
[Close]
BLAST of EY753420 vs. ExPASy Swiss-Prot
Match: CB21_GOSHI (Chlorophyll a-b binding protein 151, chloroplastic OS=Gossypium hirsutum GN=CAB-151 PE=2 SV=2)

HSP 1 Score: 72.0182 bits (175), Expect = 6.001e-23
Identity = 65/177 (36.72%), Postives = 84/177 (47.46%), Query Frame = 2 
Query:  212 VRAASTPPVKQGADRPLWFA--SKQSLSYLDGSLPGDYGFDPLGLS-DPEGTGGFIEPKWLAYGEVINGRYAMLGAVGAIAPEILGKAGLIPQETALAWFQTG-VIPPAGTYNYWADP--------YTLFVLEMALMGFAEHRRFQDWANPGSMGRQYFLGFEKYLGGSGDPAYPGG 706
            V++A T  +  G DRP +    S Q  SYL G  PGDYG+D  GLS DPE    F + + L   EVI+ R+AMLGA+G + PEIL K G+   E    WF+ G  I   G  +Y  +P          ++  ++ LMGF E  R                     LG   DP YPGG
Sbjct:   41 VKSAPTS-IWYGPDRPKYLGPFSDQIPSYLTGEFPGDYGWDTAGLSADPET---FAKNREL---EVIHCRWAMLGALGCVFPEILSKNGVKFGEA--VWFKAGSQIFSEGGLDYLGNPNLIHAQSILAIWACQVVLMGFVEGYRVGGGP----------------LGEGLDPIYPGG 192

HSP 2 Score: 56.9954 bits (136), Expect = 6.001e-23
Identity = 24/53 (45.28%), Postives = 36/53 (67.92%), Query Frame = 1 
Query:  709 SIQPLGLGKDEKSRKDFKLKEVKNGRXXMXAILGYFVQGLVTRSGALQNLWDH 867
            +  PLGL  D  +  + K+KE+KNGR  M ++ G+FVQ +VT  G ++NL+DH
Sbjct:  193 AFDPLGLADDPDAFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPIENLFDH 245
[Close]
BLAST of EY753420 vs. ExPASy Swiss-Prot
Match: CB215_PEA (Chlorophyll a-b binding protein 215, chloroplastic OS=Pisum sativum GN=CAB215 PE=1 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 6.001e-23
Identity = 61/166 (36.75%), Postives = 77/166 (46.39%), Query Frame = 2 
Query:  245 GADRPLWFA--SKQSLSYLDGSLPGDYGFDPLGLS-DPEGTGGFIEPKWLAYGEVINGRYAMLGAVGAIAPEILGKAGLIPQETALAWFQTG-VIPPAGTYNYWADP--------YTLFVLEMALMGFAEHRRFQDWANPGSMGRQYFLGFEKYLGGSGDPAYPGG 706
            G DRP +    S+Q  SYL G  PGDYG+D  GLS DPE    F   + L   EVI+ R+AMLGA+G   PE+L K G+   E    WF+ G  I   G  +Y  +P          ++  ++ LMGF E  R                     LG   DP YPGG
Sbjct:   51 GPDRPKYLGPFSEQIPSYLTGEFPGDYGWDTAGLSADPE---TFARNREL---EVIHSRWAMLGALGCTFPELLEKNGVKFGEA--VWFKAGSQIFAEGGLDYLGNPNLIHAQSILAIWATQVVLMGFVEGYRVGGGP----------------LGEGLDPLYPGG 192

HSP 2 Score: 58.9214 bits (141), Expect = 6.001e-23
Identity = 26/53 (49.06%), Postives = 36/53 (67.92%), Query Frame = 1 
Query:  709 SIQPLGLGKDEKSRKDFKLKEVKNGRXXMXAILGYFVQGLVTRSGALQNLWDH 867
            +  PLGL  D  S  + K+KE+KNGR  M ++ G+FVQ +VT  G +QNL+DH
Sbjct:  193 AFDPLGLADDPDSFAELKVKELKNGRLAMFSMFGFFVQAIVTGKGPIQNLYDH 245
[Close]
BLAST of EY753420 vs. ExPASy Swiss-Prot
Match: CB23_POLMU (Chlorophyll a-b binding protein type 1 member F3, chloroplastic OS=Polystichum munitum GN=CABF3 PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.322e-22
Identity = 60/166 (36.14%), Postives = 78/166 (46.99%), Query Frame = 2 
Query:  245 GADRPLWFA--SKQSLSYLDGSLPGDYGFDPLGLS-DPEGTGGFIEPKWLAYGEVINGRYAMLGAVGAIAPEILGKAGLIPQETALAWFQTG-VIPPAGTYNYWADP--------YTLFVLEMALMGFAEHRRFQDWANPGSMGRQYFLGFEKYLGGSGDPAYPGG 706
            G+DRPL+    S    SYL G  PGDYG+D  GLS DPE    F + + L   EVI+ R+AMLGA+G + PE+L K G+   E    WF+ G  I   G  +Y  +P          ++  ++ LMG  E  R                     LG   DP YPGG
Sbjct:   51 GSDRPLYLGPFSGSPPSYLSGEFPGDYGWDTAGLSADPET---FAKNREL---EVIHSRWAMLGALGCVTPELLAKNGVKFGEA--VWFKAGSQIFAEGGLDYLGNPSLVHAQSILAIWACQVILMGAVEGYRVAGGP----------------LGEVEDPIYPGG 192

HSP 2 Score: 56.6102 bits (135), Expect = 1.322e-22
Identity = 25/53 (47.17%), Postives = 36/53 (67.92%), Query Frame = 1 
Query:  709 SIQPLGLGKDEKSRKDFKLKEVKNGRXXMXAILGYFVQGLVTRSGALQNLWDH 867
            S  PLGL  D ++  + K+KE+KNGR  M ++ G+FVQ +VT  G ++NL DH
Sbjct:  193 SFDPLGLADDPEAFAELKVKELKNGRLAMFSMFGFFVQAIVTGKGPIENLSDH 245
[Close]
BLAST of EY753420 vs. ExPASy Swiss-Prot
Match: CB23_TOBAC (Chlorophyll a-b binding protein 36, chloroplastic OS=Nicotiana tabacum GN=CAB36 PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.322e-22
Identity = 62/162 (38.27%), Postives = 82/162 (50.62%), Query Frame = 2 
Query:  245 GADRPLWFA--SKQSLSYLDGSLPGDYGFDPLGLS-DPEGTGGFIEPKWLAYGEVINGRYAMLGAVGAIAPEILGKAGLIPQETALAWFQTG-VIPPAGTYNYWADP--------YTLFVLEMALMGFAEHRRFQDWANPGSMGRQYFLGFEK-YLGGSGDP 691
            G DRP +    S+Q+ SYL G  PGDYG+D  GLS DPE    F   + L   EVI+ R+AMLGA+G + PEIL K G+   E    WF+ G  I   G  +Y  +P          ++  ++ LMG  E  R       G +G     G +K Y GG+ DP
Sbjct:   51 GEDRPKYLGPFSEQTPSYLTGEFPGDYGWDTAGLSADPE---TFARNREL---EVIHCRWAMLGALGCVFPEILSKNGVKFGEA--VWFKAGSQIFSEGGLDYLGNPNLIHAQSILAVWASQVVLMGLIEGYR----VGGGPLGE----GLDKIYPGGAFDP 196

HSP 2 Score: 57.7658 bits (138), Expect = 1.322e-22
Identity = 24/53 (45.28%), Postives = 37/53 (69.81%), Query Frame = 1 
Query:  709 SIQPLGLGKDEKSRKDFKLKEVKNGRXXMXAILGYFVQGLVTRSGALQNLWDH 867
            +  PLGL  D ++  + K+KE+KNGR  M ++ G+FVQ +VT  G ++NL+DH
Sbjct:  193 AFDPLGLADDPEAFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPIENLFDH 245
[Close]
BLAST of EY753420 vs. ExPASy Swiss-Prot
Match: CB22_ORYSJ (Chlorophyll a-b binding protein 2, chloroplastic OS=Oryza sativa subsp. japonica GN=CAB2R PE=2 SV=2)

HSP 1 Score: 69.707 bits (169), Expect = 1.322e-22
Identity = 62/183 (33.88%), Postives = 86/183 (46.99%), Query Frame = 2 
Query:  194 RKGSFVVRAASTPPVKQGADRPLWFA--SKQSLSYLDGSLPGDYGFDPLGLS-DPEGTGGFIEPKWLAYGEVINGRYAMLGAVGAIAPEILGKAGLIPQETALAWFQTG-VIPPAGTYNYWADP--------YTLFVLEMALMGFAEHRRFQDWANPGSMGRQYFLGFEKYLGGSGDPAYPGG 706
            RK +   + A++     GADR L+    S +  SYL G  PGDYG+D  GLS DPE    F + + L   EVI+ R+AMLGA+G + PE+L + G+   E    WF+ G  I   G  +Y  +P          ++ +++ LMG  E  R                     LG   DP YPGG
Sbjct:   30 RKSAAKPKPAASGSPWYGADRVLYLGPLSGEPPSYLTGEFPGDYGWDTAGLSADPET---FAKNREL---EVIHSRWAMLGALGCVFPELLARNGVKFGEA--VWFKAGSQIFSEGGLDYLGNPSLIHAQSILAIWAVQVVLMGAVEGYRIAGGP----------------LGEVVDPLYPGG 188

HSP 2 Score: 58.151 bits (139), Expect = 1.322e-22
Identity = 26/53 (49.06%), Postives = 36/53 (67.92%), Query Frame = 1 
Query:  709 SIQPLGLGKDEKSRKDFKLKEVKNGRXXMXAILGYFVQGLVTRSGALQNLWDH 867
            S  PLGL  D ++  + K+KE+KNGR  M ++ G+FVQ +VT  G L+NL DH
Sbjct:  189 SFDPLGLADDPEAFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPLENLADH 241
[Close]
BLAST of EY753420 vs. ExPASy Swiss-Prot
Match: CB21_ORYSJ (Chlorophyll a-b binding protein 1, chloroplastic OS=Oryza sativa subsp. japonica GN=CAB1R PE=2 SV=2)

HSP 1 Score: 70.0922 bits (170), Expect = 2.238e-22
Identity = 62/175 (35.43%), Postives = 83/175 (47.43%), Query Frame = 2 
Query:  218 AASTPPVKQGADRPLWFA--SKQSLSYLDGSLPGDYGFDPLGLS-DPEGTGGFIEPKWLAYGEVINGRYAMLGAVGAIAPEILGKAGLIPQETALAWFQTG-VIPPAGTYNYWADP--------YTLFVLEMALMGFAEHRRFQDWANPGSMGRQYFLGFEKYLGGSGDPAYPGG 706
            AAS+     GADR L+    S +  SYL G  PGDYG+D  GLS DPE    F + + L   EVI+ R+AMLGA+G + PE+L + G+   E    WF+ G  I   G  +Y  +P          ++ +++ LMG  E  R                     LG   DP YPGG
Sbjct:   42 AASSGSPWYGADRVLYLGPLSGEPPSYLTGEFPGDYGWDTAGLSADPET---FAKNREL---EVIHSRWAMLGALGCVFPELLARNGVKFGEA--VWFKAGSQIFSEGGLDYLGNPSLIHAQSILAIWAVQVVLMGAVEGYRIAGGP----------------LGEVVDPLYPGG 192

HSP 2 Score: 56.9954 bits (136), Expect = 2.238e-22
Identity = 25/53 (47.17%), Postives = 36/53 (67.92%), Query Frame = 1 
Query:  709 SIQPLGLGKDEKSRKDFKLKEVKNGRXXMXAILGYFVQGLVTRSGALQNLWDH 867
            +  PLGL  D ++  + K+KE+KNGR  M ++ G+FVQ +VT  G L+NL DH
Sbjct:  193 AFDPLGLADDPEAFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPLENLADH 245
[Close]
BLAST of EY753420 vs. ExPASy Swiss-Prot
Match: CB2B_PINSY (Chlorophyll a-b binding protein type 2 member 1B, chloroplastic OS=Pinus sylvestris PE=2 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 4.925e-22
Identity = 62/175 (35.43%), Postives = 82/175 (46.86%), Query Frame = 2 
Query:  218 AASTPPVKQGADRPLWFA--SKQSLSYLDGSLPGDYGFDPLGLS-DPEGTGGFIEPKWLAYGEVINGRYAMLGAVGAIAPEILGKAGLIPQETALAWFQTGV-IPPAGTYNYWADP--------YTLFVLEMALMGFAEHRRFQDWANPGSMGRQYFLGFEKYLGGSGDPAYPGG 706
            +AST P   G DR L+    S +  SYL G  PGDYG+D  GLS DPE    F + + L   EVI+ R+AMLGA+G + PE+L + G+   E    WF+ G  I   G  +Y  +P          ++  ++ LMG  E  R                     LG   DP YPGG
Sbjct:   52 SASTSPW-YGPDRVLYLGPFSGEPPSYLTGEFPGDYGWDTAGLSADPEN---FAKNREL---EVIHCRWAMLGALGCVFPELLARNGVKFGEA--VWFKAGAQIFSEGGLDYLGNPSLVHAQSILAIWACQVILMGAVEGYRIAGGP----------------LGEVTDPIYPGG 201

HSP 2 Score: 58.151 bits (139), Expect = 4.925e-22
Identity = 25/53 (47.17%), Postives = 37/53 (69.81%), Query Frame = 1 
Query:  709 SIQPLGLGKDEKSRKDFKLKEVKNGRXXMXAILGYFVQGLVTRSGALQNLWDH 867
            S  PLGL +D ++  + K+KE+KNGR  M ++ G+FVQ +VT  G ++NL DH
Sbjct:  202 SFDPLGLAEDPEAFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPIENLADH 254
The following BLAST results are available for this feature:
BLAST of EY753420 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 70
Match NameE-valueIdentityDescription
CB2_PHYPA2.092e-2332.67Chlorophyll a-b binding protein, chloroplastic OS=... [more]
CB25_SOLLC2.731e-2338.89Chlorophyll a-b binding protein 5, chloroplastic (... [more]
CB24_SOLLC3.544e-2338.89Chlorophyll a-b binding protein 4, chloroplastic O... [more]
CB21_GOSHI6.001e-2336.72Chlorophyll a-b binding protein 151, chloroplastic... [more]
CB215_PEA6.001e-2336.75Chlorophyll a-b binding protein 215, chloroplastic... [more]
CB23_POLMU1.322e-2236.14Chlorophyll a-b binding protein type 1 member F3, ... [more]
CB23_TOBAC1.322e-2238.27Chlorophyll a-b binding protein 36, chloroplastic ... [more]
CB22_ORYSJ1.322e-2233.88Chlorophyll a-b binding protein 2, chloroplastic O... [more]
CB21_ORYSJ2.238e-2235.43Chlorophyll a-b binding protein 1, chloroplastic O... [more]
CB2B_PINSY4.925e-2235.43Chlorophyll a-b binding protein type 2 member 1B, ... [more]

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Properties
Property NameValue
Genbank descriptionCS12-C1-001-002-E12-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY753420 ID=EY753420; Name=EY753420; organism=Citrus sinensis; type=EST; length=896bp
CTTATTCTTCAAAANCAGAAGCAGAAAGCCGAAGCACTTTTAATTTATCG
TATTGAAGTAGAGAAACAAGAGGAAAAAAGAAAATTAAAAAATGGCAACC
CAAGCTTTGGTTTCATCATCTCTTACATCTTCAGTGGAGACTGCGAGACA
AATTCTTGGAGCAAGATCAGCTCAATCCCCAACTGGGTCATCAAGAAAAG
GTTCCTTTGTTGTTAGGGCAGCTTCTACTCCTCCTGTTAAGCAAGGAGCA
GACAGACCGTTGTGGTTTGCATCCAAACAAAGCCTTTCATACTTGGATGG
CAGCCTCCCCGGAGACTATGGATTCGACCCACTTGGTCTCTCAGACCCTG
AAGGCACAGGAGGGTTCATTGAGCCAAAATGGCTGGCCTACGGCGAGGTC
ATCAACGGTCGTTATGCCATGTTGGGAGCTGTGGGAGCCATTGCACCGGA
GATTCTTGGCAAGGCAGGCCTCATTCCGCAAGAAACTGCCCTGGCCTGGT
TCCAAACCGGTGTGATCCCCCCAGCGGGAACATACAACTACTGGGCAGAC
CCTTACACACTTTTCGTCCTGGAAATGGCACTCATGGGATTTGCCGAGCA
CCGGAGATTCCAAGACTGGGCAAATCCAGGATCAATGGGCAGGCAATACT
TCCTCGGATTCGAGAAATACTTGGGAGGATCCGGCGATCCGGCTTACCCT
GGAGGACCTCTATTCAACCTCTGGGTCTGGGGAAAGATGAGAAGTCAAGG
AAGGATTTCAAGCTCAAGGAAGTGAAGAATGGAAGATNGNNCATGNTNGC
AATCTTGGGGTACTTTGTTCAAGGTCTTGTTACAAGGAGTGGGGCCTTAC
AGAACCTATGGGATCACTGGCTGATCCTGTAACAACATGTTTTGAC
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