EY720600 | Citrus Genome Database

Overview


Name	EY720600
Unique Name	EY720600
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	953

Libraries


Library Name	Type
Sweet orange fruit, development stadium (4 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_SCHCO (Elongation factor 1-alpha OS=Schizophyllum commune GN=TEF1 PE=3 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 8.529e-30
Identity = 59/86 (68.60%), Postives = 70/86 (81.40%), Query Frame = 2

Query:   80 RRRLTSTLMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            + +L   ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEAA++   SFKYA VLD+L AERER ITIDI LWKFE+ +Y  T
Sbjct:    3 KEKLHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYMVT 88

HSP 2 Score: 30.8018 bits (68), Expect = 8.529e-30
Identity = 17/35 (48.57%), Postives = 23/35 (65.71%), Query Frame = 3

Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSGG 446
            ++APG R + KNMITG S    +  C+ ILT +GG
Sbjct:   90 IDAPGHRDFIKNMITGTS----QADCA-ILTIAGG 119

BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_PEA (Elongation factor 1-alpha OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 8.533e-30
Identity = 61/86 (70.93%), Postives = 73/86 (84.88%), Query Frame = 2

Query:   80 RRRLTSTLMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            + ++   ++VIGHVDSGKSTTT H+IYKLGGIDKRVI+RFEKEA +MN RSFKYA +LD+L AERER ITIDI L KFE+T+YY T
Sbjct:    3 KEKVHINIVVIGHVDSGKSTTTVHVIYKLGGIDKRVIERFEKEADEMNKRSFKYAWLLDKLKAERERGITIDIALLKFETTKYYST 88

HSP 2 Score: 28.4906 bits (62), Expect = 8.533e-30
Identity = 17/41 (41.46%), Postives = 24/41 (58.54%), Query Frame = 3

Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFIL---TFSGGEAG 455
            ++APG R + KNMITG S    +  C+ ++   T  G EAG
Sbjct:   90 MDAPGHRDFIKNMITGTS----QADCAVLIIDSTTGGFEAG 126

BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_PUCGR (Elongation factor 1-alpha OS=Puccinia graminis GN=TEF PE=3 SV=2)

HSP 1 Score: 122.865 bits (307), Expect = 1.446e-29
Identity = 59/79 (74.68%), Postives = 68/79 (86.08%), Query Frame = 2

Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEAA++   SFKYA VLD+L AERER ITIDI LWKFE+ +YY T
Sbjct:   10 VVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYYVT 88

HSP 2 Score: 28.4906 bits (62), Expect = 1.446e-29
Identity = 13/34 (38.24%), Postives = 21/34 (61.76%), Query Frame = 3

Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSG 443
            ++APG R + KNMITG S    +  C+ ++  +G
Sbjct:   90 IDAPGHRDFIKNMITGTS----QADCAILIIAAG 119

BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_PODCU (Elongation factor 1-alpha OS=Podospora curvicolla GN=TEF PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 1.446e-29
Identity = 59/79 (74.68%), Postives = 68/79 (86.08%), Query Frame = 2

Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEAA++   SFKYA VLD+L AERER ITIDI LWKFE+ +YY T
Sbjct:   11 VVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYYVT 89

HSP 2 Score: 28.4906 bits (62), Expect = 1.446e-29
Identity = 13/34 (38.24%), Postives = 21/34 (61.76%), Query Frame = 3

Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSG 443
            ++APG R + KNMITG S    +  C+ ++  +G
Sbjct:   91 IDAPGHRDFIKNMITGTS----QADCAILIIAAG 120

BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_SORMA (Elongation factor 1-alpha OS=Sordaria macrospora GN=TEF PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 1.446e-29
Identity = 59/79 (74.68%), Postives = 68/79 (86.08%), Query Frame = 2

Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEAA++   SFKYA VLD+L AERER ITIDI LWKFE+ +YY T
Sbjct:   11 VVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYYVT 89

HSP 2 Score: 28.4906 bits (62), Expect = 1.446e-29
Identity = 13/34 (38.24%), Postives = 21/34 (61.76%), Query Frame = 3

Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSG 443
            ++APG R + KNMITG S    +  C+ ++  +G
Sbjct:   91 IDAPGHRDFIKNMITGTS----QADCAILIIAAG 120

BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_PODAN (Elongation factor 1-alpha OS=Podospora anserina GN=TEF PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 1.446e-29
Identity = 59/79 (74.68%), Postives = 68/79 (86.08%), Query Frame = 2

Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEAA++   SFKYA VLD+L AERER ITIDI LWKFE+ +YY T
Sbjct:   11 VVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYYVT 89

HSP 2 Score: 28.4906 bits (62), Expect = 1.446e-29
Identity = 13/34 (38.24%), Postives = 21/34 (61.76%), Query Frame = 3

Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSG 443
            ++APG R + KNMITG S    +  C+ ++  +G
Sbjct:   91 IDAPGHRDFIKNMITGTS----QADCAILIIAAG 120

BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_NEUCR (Elongation factor 1-alpha OS=Neurospora crassa GN=tef-1 PE=3 SV=2)

HSP 1 Score: 122.865 bits (307), Expect = 1.446e-29
Identity = 59/79 (74.68%), Postives = 68/79 (86.08%), Query Frame = 2

Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEAA++   SFKYA VLD+L AERER ITIDI LWKFE+ +YY T
Sbjct:   11 VVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYYVT 89

HSP 2 Score: 28.4906 bits (62), Expect = 1.446e-29
Identity = 13/34 (38.24%), Postives = 21/34 (61.76%), Query Frame = 3

Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSG 443
            ++APG R + KNMITG S    +  C+ ++  +G
Sbjct:   91 IDAPGHRDFIKNMITGTS----QADCAILIIAAG 120

BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_ARXAD (Elongation factor 1-alpha OS=Arxula adeninivorans GN=TEF PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 1.446e-29
Identity = 59/79 (74.68%), Postives = 68/79 (86.08%), Query Frame = 2

Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEAA++   SFKYA VLD+L AERER ITIDI LWKFE+ +YY T
Sbjct:   10 VVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERVITIDIALWKFETPKYYVT 88

HSP 2 Score: 28.4906 bits (62), Expect = 1.446e-29
Identity = 16/35 (45.71%), Postives = 22/35 (62.86%), Query Frame = 3

Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSGG 446
            ++APG R + KNMITG S    +  C+ IL  +GG
Sbjct:   90 IDAPGHRDFIKNMITGTS----QADCA-ILIIAGG 119

BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_ENTHI (Elongation factor 1-alpha OS=Entamoeba histolytica PE=2 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 1.651e-29
Identity = 58/79 (73.42%), Postives = 69/79 (87.34%), Query Frame = 2

Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            ++VIGHVDSGKSTTTGHLIYK GGID+R I++FEKE+A+M   SFKYA VLD L AERER ITIDI LWKFE+++YY+T
Sbjct:   10 IVVIGHVDSGKSTTTGHLIYKCGGIDQRTIEKFEKESAEMGKGSFKYAWVLDNLKAERERGITIDISLWKFETSKYYFT 88

HSP 2 Score: 27.7202 bits (60), Expect = 1.651e-29
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3

Query:  342 VEAPGRRQWRKNMITGNS 395
            ++APG R + KNMITG S
Sbjct:   90 IDAPGHRDFIKNMITGTS 107

HSP 3 Score: 20.7866 bits (42), Expect = 1.651e-29
Identity = 8/8 (100.00%), Postives = 8/8 (100.00%), Query Frame = 1

Query:   79 KEKTHINI 102
            KEKTHINI
Sbjct:    3 KEKTHINI 10

BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_CAEEL (Elongation factor 1-alpha OS=Caenorhabditis elegans GN=eft-3 PE=2 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.882e-29
Identity = 59/86 (68.60%), Postives = 70/86 (81.40%), Query Frame = 2

Query:   80 RRRLTSTLMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            + ++   ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEA +M   SFKYA VLD+L AERER ITIDI LWKFE+ +YY T
Sbjct:    3 KEKVHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYIT 88

HSP 2 Score: 27.7202 bits (60), Expect = 1.882e-29
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3

Query:  342 VEAPGRRQWRKNMITGNS 395
            ++APG R + KNMITG S
Sbjct:   90 IDAPGHRDFIKNMITGTS 107

The following BLAST results are available for this feature:

BLAST of EY720600 vs. ExPASy Swiss-Prot

BLAST of EY720600 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 158


Match Name	E-value	Identity	Description
EF1A_SCHCO	8.529e-30	68.60	Elongation factor 1-alpha OS=Schizophyllum commune...	[more]
EF1A_PEA	8.533e-30	70.93	Elongation factor 1-alpha OS=Pisum sativum PE=2 SV...	[more]
EF1A_PUCGR	1.446e-29	74.68	Elongation factor 1-alpha OS=Puccinia graminis GN=...	[more]
EF1A_PODCU	1.446e-29	74.68	Elongation factor 1-alpha OS=Podospora curvicolla ...	[more]
EF1A_SORMA	1.446e-29	74.68	Elongation factor 1-alpha OS=Sordaria macrospora G...	[more]
EF1A_PODAN	1.446e-29	74.68	Elongation factor 1-alpha OS=Podospora anserina GN...	[more]
EF1A_NEUCR	1.446e-29	74.68	Elongation factor 1-alpha OS=Neurospora crassa GN=...	[more]
EF1A_ARXAD	1.446e-29	74.68	Elongation factor 1-alpha OS=Arxula adeninivorans ...	[more]
EF1A_ENTHI	1.651e-29	73.42	Elongation factor 1-alpha OS=Entamoeba histolytica...	[more]
EF1A_CAEEL	1.882e-29	68.60	Elongation factor 1-alpha OS=Caenorhabditis elegan...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C3-703-011-G02-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY720600 ID=EY720600; Name=EY720600; organism=Citrus sinensis; type=EST; length=953bp
CACGCGTCCGGCGGCGAGAGAGAACCATTTTCGCGAATATCATTTTAATA
AGGTTTTTTCTAGATGGGCATGATGGGTAAGGAGAAGACTCACATCAACA
TTGATGGTCATCGGCCATGTCGACTCTGGAAAGTCGACCACCACCGGACA
CTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCAAGAGGTTCG
AAAAGGAAGCTGCTGATATGAACGATAGGTCATTCAAATACGCCTGCGTG
CTCGACGAGCTCCATGCTGAGCGTGAACGTGCTATTACCATTGATATTGT
CTTGTGGAAGTTCGAGAGCACCGAGTACTACTGGACGCGGCGTTGAGGCT
CCTGGACGTCGGCAGTGGAGGAAGAACATGATTACCGGTAACTCAACAGA
CTGACTGGAACATCCTTGTTCGTTTATTCTTACTTTCAGTGGTGGGGAAG
CGGGGGACAGGGAGGGATGGAGGCGAGCGTGGAGGGTGTGGGTTGGCGCG
TCGGTGATGGGGGGGAGCGTGTCGTTGCGCGGGGGGGGGGCAGGGTGGGG
GCCCGCGGAGGTGGGCGGGGGTGGGGGGGCGGAGCGGTGGCGGGCCGGGC
GGGGGCGGATGCCGGGCGGGGGGGCGGGGGTCCTGGGGGGGCGGGGGAGG
AGGGGGGGTGGGTGGGGGAGGGAGCGCGGGGGGGGGGGGGGTGGCGGTGG
GCGGGGGAGGGTGGTGGGGGAGAGTGGGGGGCGGGCGGTGGCGGAGGCAC
GGAGAGGGGGAGAGGGACGGTGAGGCGAGGTGGGTTTGAGTGGGAGGGCC
GCGTGGCGGGGGAGGGTTCGCAGGGGGGGCGGTGGGTGGGAGAGGGGGGG
TTGCGTTGAGAGGGAGGAGGGTGGAGGGAGGGGGGTGGGGGGGATGGGGG
TGAGGGGGGCGAGGCGGGGAGGCGGCGGGGGGTGGGGTGCGGGGGGGCGG
GGG