EY720600

Overview
NameEY720600
Unique NameEY720600
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length953
Libraries
Library NameType
Sweet orange fruit, development stadium (4 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_ASHGO (Elongation factor 1-alpha OS=Ashbya gossypii GN=TEF PE=3 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 5.410e-29
Identity = 58/79 (73.42%), Postives = 68/79 (86.08%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEAA++   SFKYA VLD+L AERER ITIDI LWKFE+ +Y+ T
Sbjct:   10 VVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYHVT 88

HSP 2 Score: 28.4906 bits (62), Expect = 5.410e-29
Identity = 16/35 (45.71%), Postives = 22/35 (62.86%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSGG 446
            ++APG R + KNMITG S    +  C+ IL  +GG
Sbjct:   90 IDAPGHRDFIKNMITGTS----QADCA-ILIIAGG 119
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A3_OSCTI (Elongation factor 1-alpha 3 OS=Oscheius tipulae GN=eft-3 PE=3 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 7.043e-29
Identity = 58/79 (73.42%), Postives = 67/79 (84.81%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEA +M   SFKYA VLD+L AERER ITIDI LWKFE+ ++Y T
Sbjct:   11 IVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKFYVT 89

HSP 2 Score: 27.7202 bits (60), Expect = 7.043e-29
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNS 395
            ++APG R + KNMITG S
Sbjct:   91 IDAPGHRDFIKNMITGTS 108
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_ABSGL (Elongation factor 1-alpha OS=Absidia glauca GN=TEF-1 PE=3 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 7.043e-29
Identity = 58/79 (73.42%), Postives = 68/79 (86.08%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEAA++   SFKYA VLD+L AERER ITIDI LWKFE+ +Y+ T
Sbjct:   10 VVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYHVT 88

HSP 2 Score: 28.1054 bits (61), Expect = 7.043e-29
Identity = 13/34 (38.24%), Postives = 20/34 (58.82%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSG 443
            ++APG R + KNMITG S    +  C  ++  +G
Sbjct:   90 IDAPGHRDFIKNMITGTS----QADCGILIIAAG 119
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_PORYE (Elongation factor 1-alpha OS=Porphyra yezoensis PE=2 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 7.045e-29
Identity = 58/80 (72.50%), Postives = 69/80 (86.25%), Query Frame = 2 
Query:   98 TLMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            +++VIGHVDSGKSTTTGHLIYK GGI+KR I++FEKEAA+M   SFKYA VLD+L AERER ITIDI LWKFE+ +Y +T
Sbjct:    9 SIVVIGHVDSGKSTTTGHLIYKCGGIEKRAIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETEKYSFT 88

HSP 2 Score: 27.7202 bits (60), Expect = 7.045e-29
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNS 395
            ++APG R + KNMITG S
Sbjct:   90 IDAPGHRDFIKNMITGTS 107
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_ONCVO (Elongation factor 1-alpha OS=Onchocerca volvulus PE=2 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 1.024e-28
Identity = 58/79 (73.42%), Postives = 66/79 (83.54%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEA +M   SFKYA VLD+L AERER I IDI LWKFE+ +YY T
Sbjct:   10 IVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGIQIDIALWKFETPKYYIT 88

HSP 2 Score: 27.7202 bits (60), Expect = 1.024e-28
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNS 395
            ++APG R + KNMITG S
Sbjct:   90 IDAPGHRDFIKNMITGTS 107

HSP 3 Score: 20.7866 bits (42), Expect = 1.024e-28
Identity = 8/8 (100.00%), Postives = 8/8 (100.00%), Query Frame = 1 
Query:   79 KEKTHINI 102
            KEKTHINI
Sbjct:    3 KEKTHINI 10
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_YEAST (Elongation factor 1-alpha OS=Saccharomyces cerevisiae GN=TEF1 PE=1 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 1.194e-28
Identity = 58/79 (73.42%), Postives = 67/79 (84.81%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEAA++   SFKYA VLD+L AERER ITIDI LWKFE+ +Y  T
Sbjct:   10 VVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYQVT 88

HSP 2 Score: 28.4906 bits (62), Expect = 1.194e-28
Identity = 16/35 (45.71%), Postives = 22/35 (62.86%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSGG 446
            ++APG R + KNMITG S    +  C+ IL  +GG
Sbjct:   90 IDAPGHRDFIKNMITGTS----QADCA-ILIIAGG 119
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_COCIM (Elongation factor 1-alpha OS=Coccidioides immitis GN=TEF PE=2 SV=2)

HSP 1 Score: 119.783 bits (299), Expect = 1.194e-28
Identity = 57/79 (72.15%), Postives = 67/79 (84.81%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            L+VIGHVDSGKSTTTGHLIYK GGID R I++FEKEA ++  +SFKYA VLD+L AERER ITIDI LWKFE+ +Y+ T
Sbjct:   11 LVVIGHVDSGKSTTTGHLIYKCGGIDNRTIEKFEKEAEELGKKSFKYAWVLDKLKAERERGITIDIALWKFETPKYHVT 89

HSP 2 Score: 28.4906 bits (62), Expect = 1.194e-28
Identity = 13/34 (38.24%), Postives = 21/34 (61.76%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSG 443
            ++APG R + KNMITG S    +  C+ ++  +G
Sbjct:   91 IDAPGHRDFIKNMITGTS----QADCAILIIAAG 120
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_YARLI (Elongation factor 1-alpha OS=Yarrowia lipolytica GN=TEF PE=2 SV=2)

HSP 1 Score: 119.398 bits (298), Expect = 1.554e-28
Identity = 57/79 (72.15%), Postives = 67/79 (84.81%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            L+VIGHVD+GKSTTTGHLIYK GGIDKR I++FEKEA ++   SFKYA VLD+L AERER ITIDI LWKF++ +YY T
Sbjct:   10 LVVIGHVDAGKSTTTGHLIYKCGGIDKRTIEKFEKEADELGKGSFKYAWVLDKLKAERERGITIDIALWKFQTPKYYVT 88

HSP 2 Score: 28.4906 bits (62), Expect = 1.554e-28
Identity = 16/35 (45.71%), Postives = 22/35 (62.86%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSGG 446
            ++APG R + KNMITG S    +  C+ IL  +GG
Sbjct:   90 IDAPGHRDFIKNMITGTS----QADCA-ILIIAGG 119
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A2_RHIRA (Elongation factor 1-alpha OS=Rhizomucor racemosus GN=TEF-2 PE=3 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 1.554e-28
Identity = 57/76 (75.00%), Postives = 66/76 (86.84%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEY 328
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEAA++   SFKYA VLD+L AERER ITIDI LWKFE+ +Y
Sbjct:   10 VVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKY 85

HSP 2 Score: 28.4906 bits (62), Expect = 1.554e-28
Identity = 16/35 (45.71%), Postives = 22/35 (62.86%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSGG 446
            ++APG R + KNMITG S    +  C+ IL  +GG
Sbjct:   90 IDAPGHRDFIKNMITGTS----QADCA-ILIIAGG 119
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A3_RHIRA (Elongation factor 1-alpha OS=Rhizomucor racemosus GN=TEF-3 PE=3 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 1.554e-28
Identity = 57/76 (75.00%), Postives = 66/76 (86.84%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEY 328
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEAA++   SFKYA VLD+L AERER ITIDI LWKFE+ +Y
Sbjct:   10 VVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKY 85

HSP 2 Score: 28.4906 bits (62), Expect = 1.554e-28
Identity = 16/35 (45.71%), Postives = 22/35 (62.86%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSGG 446
            ++APG R + KNMITG S    +  C+ IL  +GG
Sbjct:   90 IDAPGHRDFIKNMITGTS----QADCA-ILIIAGG 119
The following BLAST results are available for this feature:
BLAST of EY720600 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 158
Match NameE-valueIdentityDescription
EF1A_ASHGO5.410e-2973.42Elongation factor 1-alpha OS=Ashbya gossypii GN=TE... [more]
EF1A3_OSCTI7.043e-2973.42Elongation factor 1-alpha 3 OS=Oscheius tipulae GN... [more]
EF1A_ABSGL7.043e-2973.42Elongation factor 1-alpha OS=Absidia glauca GN=TEF... [more]
EF1A_PORYE7.045e-2972.50Elongation factor 1-alpha OS=Porphyra yezoensis PE... [more]
EF1A_ONCVO1.024e-2873.42Elongation factor 1-alpha OS=Onchocerca volvulus P... [more]
EF1A_YEAST1.194e-2873.42Elongation factor 1-alpha OS=Saccharomyces cerevis... [more]
EF1A_COCIM1.194e-2872.15Elongation factor 1-alpha OS=Coccidioides immitis ... [more]
EF1A_YARLI1.554e-2872.15Elongation factor 1-alpha OS=Yarrowia lipolytica G... [more]
EF1A2_RHIRA1.554e-2875.00Elongation factor 1-alpha OS=Rhizomucor racemosus ... [more]
EF1A3_RHIRA1.554e-2875.00Elongation factor 1-alpha OS=Rhizomucor racemosus ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-703-011-G02-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY720600 ID=EY720600; Name=EY720600; organism=Citrus sinensis; type=EST; length=953bp
CACGCGTCCGGCGGCGAGAGAGAACCATTTTCGCGAATATCATTTTAATA
AGGTTTTTTCTAGATGGGCATGATGGGTAAGGAGAAGACTCACATCAACA
TTGATGGTCATCGGCCATGTCGACTCTGGAAAGTCGACCACCACCGGACA
CTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCAAGAGGTTCG
AAAAGGAAGCTGCTGATATGAACGATAGGTCATTCAAATACGCCTGCGTG
CTCGACGAGCTCCATGCTGAGCGTGAACGTGCTATTACCATTGATATTGT
CTTGTGGAAGTTCGAGAGCACCGAGTACTACTGGACGCGGCGTTGAGGCT
CCTGGACGTCGGCAGTGGAGGAAGAACATGATTACCGGTAACTCAACAGA
CTGACTGGAACATCCTTGTTCGTTTATTCTTACTTTCAGTGGTGGGGAAG
CGGGGGACAGGGAGGGATGGAGGCGAGCGTGGAGGGTGTGGGTTGGCGCG
TCGGTGATGGGGGGGAGCGTGTCGTTGCGCGGGGGGGGGGCAGGGTGGGG
GCCCGCGGAGGTGGGCGGGGGTGGGGGGGCGGAGCGGTGGCGGGCCGGGC
GGGGGCGGATGCCGGGCGGGGGGGCGGGGGTCCTGGGGGGGCGGGGGAGG
AGGGGGGGTGGGTGGGGGAGGGAGCGCGGGGGGGGGGGGGGTGGCGGTGG
GCGGGGGAGGGTGGTGGGGGAGAGTGGGGGGCGGGCGGTGGCGGAGGCAC
GGAGAGGGGGAGAGGGACGGTGAGGCGAGGTGGGTTTGAGTGGGAGGGCC
GCGTGGCGGGGGAGGGTTCGCAGGGGGGGCGGTGGGTGGGAGAGGGGGGG
TTGCGTTGAGAGGGAGGAGGGTGGAGGGAGGGGGGTGGGGGGGATGGGGG
TGAGGGGGGCGAGGCGGGGAGGCGGCGGGGGGTGGGGTGCGGGGGGGCGG
GGG
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