EY720600

Overview
NameEY720600
Unique NameEY720600
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length953
Libraries
Library NameType
Sweet orange fruit, development stadium (4 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_EUGGR (Elongation factor 1-alpha OS=Euglena gracilis GN=TEF PE=2 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 1.555e-28
Identity = 58/86 (67.44%), Postives = 71/86 (82.56%), Query Frame = 2 
Query:   80 RRRLTSTLMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            + ++  +L+VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEA++M   SFKYA VLD+L AERER ITIDI LWKFE+ +  +T
Sbjct:    3 KEKVHISLVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEASEMGKGSFKYAWVLDKLKAERERCITIDIALWKFETAKSVFT 88

HSP 2 Score: 27.7202 bits (60), Expect = 1.555e-28
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNS 395
            ++APG R + KNMITG S
Sbjct:   90 IDAPGHRDFIKNMITGTS 107
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A1_EUPCR (Elongation factor 1-alpha 1 OS=Euplotes crassus GN=EFA1 PE=3 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 1.555e-28
Identity = 58/79 (73.42%), Postives = 67/79 (84.81%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            L+VIGHVDSGKSTTTGHLIYKLGGID R I++FEKE+A+M   SFKYA VLD+L AERER ITIDI LWKFE+   ++T
Sbjct:   10 LVVIGHVDSGKSTTTGHLIYKLGGIDARTIEKFEKESAEMGKASFKYAWVLDKLKAERERGITIDIALWKFETENRHYT 88

HSP 2 Score: 27.7202 bits (60), Expect = 1.555e-28
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNS 395
            ++APG R + KNMITG S
Sbjct:   90 IDAPGHRDFIKNMITGTS 107
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A1_OSCTI (Elongation factor 1-alpha 1 OS=Oscheius tipulae GN=eft-1 PE=3 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 2.242e-28
Identity = 58/79 (73.42%), Postives = 67/79 (84.81%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEA +M   SFKYA VLD+L AERER ITIDI LWKFE+ ++Y T
Sbjct:   10 IVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKFYVT 88

HSP 2 Score: 25.409 bits (54), Expect = 2.242e-28
Identity = 10/18 (55.56%), Postives = 13/18 (72.22%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNS 395
            ++APG R + K MITG S
Sbjct:   90 IDAPGHRDFIKKMITGTS 107

HSP 3 Score: 20.7866 bits (42), Expect = 2.242e-28
Identity = 8/8 (100.00%), Postives = 8/8 (100.00%), Query Frame = 1 
Query:   79 KEKTHINI 102
            KEKTHINI
Sbjct:    3 KEKTHINI 10
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_CRYNE (Elongation factor 1-alpha OS=Cryptococcus neoformans GN=TEF1 PE=2 SV=2)

HSP 1 Score: 118.627 bits (296), Expect = 2.634e-28
Identity = 58/84 (69.05%), Postives = 67/84 (79.76%), Query Frame = 2 
Query:   86 RLTSTLMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            +L   ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEA ++   SFKYA VLD+L AERER ITIDI LWKFE+  Y  T
Sbjct:    5 KLHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQELGKSSFKYAWVLDKLKAERERGITIDIALWKFETPRYQVT 88

HSP 2 Score: 28.4906 bits (62), Expect = 2.634e-28
Identity = 13/34 (38.24%), Postives = 21/34 (61.76%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSG 443
            ++APG R + KNMITG S    +  C+ ++  +G
Sbjct:   90 IDAPGHRDFIKNMITGTS----QADCAILIIATG 119
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_AURPU (Elongation factor 1-alpha OS=Aureobasidium pullulans GN=TEF1 PE=3 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 2.634e-28
Identity = 57/79 (72.15%), Postives = 67/79 (84.81%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEAA++   SFKYA VLD+L +ERER ITIDI LWKFE+ +Y  T
Sbjct:   10 VVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKSERERGITIDIALWKFETPKYMVT 88

HSP 2 Score: 28.4906 bits (62), Expect = 2.634e-28
Identity = 13/34 (38.24%), Postives = 21/34 (61.76%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSG 443
            ++APG R + KNMITG S    +  C+ ++  +G
Sbjct:   90 IDAPGHRDFIKNMITGTS----QADCAILIIAAG 119
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A1_RHIRA (Elongation factor 1-alpha OS=Rhizomucor racemosus GN=TEF-1 PE=3 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 2.634e-28
Identity = 57/76 (75.00%), Postives = 65/76 (85.53%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEY 328
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I+ FEKEAA++   SFKYA VLD+L AERER ITIDI LWKFE+ +Y
Sbjct:   10 VVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEEFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKY 85

HSP 2 Score: 28.4906 bits (62), Expect = 2.634e-28
Identity = 16/35 (45.71%), Postives = 22/35 (62.86%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSGG 446
            ++APG R + KNMITG S    +  C+ IL  +GG
Sbjct:   90 IDAPGHRDFIKNMITGTS----QADCA-ILIIAGG 119
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A2_SCHPO (Elongation factor 1-alpha-B/C OS=Schizosaccharomyces pombe GN=tef1b PE=1 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 2.634e-28
Identity = 56/76 (73.68%), Postives = 65/76 (85.53%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEY 328
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEA ++   SFKYA VLD+L AERER ITIDI LWKFE+ +Y
Sbjct:   10 VVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKY 85

HSP 2 Score: 29.261 bits (64), Expect = 2.634e-28
Identity = 16/41 (39.02%), Postives = 24/41 (58.54%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSGGEAGDRE 464
            ++APG R + KNMITG S    +  C+ ++   GG  G+ E
Sbjct:   90 IDAPGHRDFIKNMITGTS----QADCAILII--GGGTGEFE 124
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_TRYBB (Elongation factor 1-alpha OS=Trypanosoma brucei brucei GN=TEF1 PE=1 SV=3)

HSP 1 Score: 119.013 bits (297), Expect = 2.635e-28
Identity = 57/86 (66.28%), Postives = 69/86 (80.23%), Query Frame = 2 
Query:   80 RRRLTSTLMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            + ++   L+V+GHVD+GKST TGHLIYK GGIDKR I++FEKEAAD+   SFKYA VLD+L AERER ITIDI LWKFES +  +T
Sbjct:    3 KEKVHMNLVVVGHVDAGKSTATGHLIYKCGGIDKRTIEKFEKEAADIGKASFKYAWVLDKLKAERERGITIDIALWKFESPKSVFT 88

HSP 2 Score: 28.1054 bits (61), Expect = 2.635e-28
Identity = 12/26 (46.15%), Postives = 17/26 (65.38%), Query Frame = 3 
Query:  318 APSTTGRGVEAPGRRQWRKNMITGNS 395
            +P +    ++APG R + KNMITG S
Sbjct:   82 SPKSVFTIIDAPGHRDFIKNMITGTS 107
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A1_SCHPO (Elongation factor 1-alpha-A OS=Schizosaccharomyces pombe GN=tef1a PE=1 SV=2)

HSP 1 Score: 117.857 bits (294), Expect = 3.430e-28
Identity = 56/76 (73.68%), Postives = 65/76 (85.53%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEY 328
            ++VIGHVDSGKSTTTGHLIYK GGIDKR I++FEKEA ++   SFKYA VLD+L AERER ITIDI LWKFE+ +Y
Sbjct:   10 VVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKY 85

HSP 2 Score: 28.8758 bits (63), Expect = 3.430e-28
Identity = 16/41 (39.02%), Postives = 24/41 (58.54%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSGGEAGDRE 464
            ++APG R + KNMITG S    +  C+ ++   GG  G+ E
Sbjct:   90 IDAPGHRDFIKNMITGTS----QADCAVLII--GGGTGEFE 124
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_HYDAT (Elongation factor 1-alpha OS=Hydra attenuata PE=2 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 4.463e-28
Identity = 57/79 (72.15%), Postives = 67/79 (84.81%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            ++VIGHVDSGKST+TGH+IYK GGIDKR I++FEKEA +M   SFKYA VLD+L AERER ITIDI LWKFE+T+Y  T
Sbjct:   11 IVVIGHVDSGKSTSTGHMIYKCGGIDKRQIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETTKYVVT 89

HSP 2 Score: 27.7202 bits (60), Expect = 4.463e-28
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNS 395
            ++APG R + KNMITG S
Sbjct:   91 IDAPGHRDFIKNMITGTS 108
The following BLAST results are available for this feature:
BLAST of EY720600 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 158
Match NameE-valueIdentityDescription
EF1A_EUGGR1.555e-2867.44Elongation factor 1-alpha OS=Euglena gracilis GN=T... [more]
EF1A1_EUPCR1.555e-2873.42Elongation factor 1-alpha 1 OS=Euplotes crassus GN... [more]
EF1A1_OSCTI2.242e-2873.42Elongation factor 1-alpha 1 OS=Oscheius tipulae GN... [more]
EF1A_CRYNE2.634e-2869.05Elongation factor 1-alpha OS=Cryptococcus neoforma... [more]
EF1A_AURPU2.634e-2872.15Elongation factor 1-alpha OS=Aureobasidium pullula... [more]
EF1A1_RHIRA2.634e-2875.00Elongation factor 1-alpha OS=Rhizomucor racemosus ... [more]
EF1A2_SCHPO2.634e-2873.68Elongation factor 1-alpha-B/C OS=Schizosaccharomyc... [more]
EF1A_TRYBB2.635e-2866.28Elongation factor 1-alpha OS=Trypanosoma brucei br... [more]
EF1A1_SCHPO3.430e-2873.68Elongation factor 1-alpha-A OS=Schizosaccharomyces... [more]
EF1A_HYDAT4.463e-2872.15Elongation factor 1-alpha OS=Hydra attenuata PE=2 ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-703-011-G02-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY720600 ID=EY720600; Name=EY720600; organism=Citrus sinensis; type=EST; length=953bp
CACGCGTCCGGCGGCGAGAGAGAACCATTTTCGCGAATATCATTTTAATA
AGGTTTTTTCTAGATGGGCATGATGGGTAAGGAGAAGACTCACATCAACA
TTGATGGTCATCGGCCATGTCGACTCTGGAAAGTCGACCACCACCGGACA
CTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCAAGAGGTTCG
AAAAGGAAGCTGCTGATATGAACGATAGGTCATTCAAATACGCCTGCGTG
CTCGACGAGCTCCATGCTGAGCGTGAACGTGCTATTACCATTGATATTGT
CTTGTGGAAGTTCGAGAGCACCGAGTACTACTGGACGCGGCGTTGAGGCT
CCTGGACGTCGGCAGTGGAGGAAGAACATGATTACCGGTAACTCAACAGA
CTGACTGGAACATCCTTGTTCGTTTATTCTTACTTTCAGTGGTGGGGAAG
CGGGGGACAGGGAGGGATGGAGGCGAGCGTGGAGGGTGTGGGTTGGCGCG
TCGGTGATGGGGGGGAGCGTGTCGTTGCGCGGGGGGGGGGCAGGGTGGGG
GCCCGCGGAGGTGGGCGGGGGTGGGGGGGCGGAGCGGTGGCGGGCCGGGC
GGGGGCGGATGCCGGGCGGGGGGGCGGGGGTCCTGGGGGGGCGGGGGAGG
AGGGGGGGTGGGTGGGGGAGGGAGCGCGGGGGGGGGGGGGGTGGCGGTGG
GCGGGGGAGGGTGGTGGGGGAGAGTGGGGGGCGGGCGGTGGCGGAGGCAC
GGAGAGGGGGAGAGGGACGGTGAGGCGAGGTGGGTTTGAGTGGGAGGGCC
GCGTGGCGGGGGAGGGTTCGCAGGGGGGGCGGTGGGTGGGAGAGGGGGGG
TTGCGTTGAGAGGGAGGAGGGTGGAGGGAGGGGGGTGGGGGGGATGGGGG
TGAGGGGGGCGAGGCGGGGAGGCGGCGGGGGGTGGGGTGCGGGGGGGCGG
GGG
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