EY720600

Overview
NameEY720600
Unique NameEY720600
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length953
Libraries
Library NameType
Sweet orange fruit, development stadium (4 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_HELAL (Elongation factor 1-alpha (Fragment) OS=Heliocheilus albipunctella PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.285e-27
Identity = 56/74 (75.68%), Postives = 62/74 (83.78%), Query Frame = 2 
Query:  116 HVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            HVDSGKSTTTGHLIYK GGIDKR I++FEKEA +M   SFKYA VLD+L AERER ITIDI LWKFE+ +YY T
Sbjct:    1 HVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVT 74

HSP 2 Score: 28.4906 bits (62), Expect = 1.285e-27
Identity = 13/34 (38.24%), Postives = 21/34 (61.76%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSG 443
            ++APG R + KNMITG S    +  C+ ++  +G
Sbjct:   76 IDAPGHRDFIKNMITGTS----QADCAVLIVAAG 105
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_ANIIF (Elongation factor 1-alpha (Fragment) OS=Anicla infecta PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.285e-27
Identity = 56/74 (75.68%), Postives = 62/74 (83.78%), Query Frame = 2 
Query:  116 HVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            HVDSGKSTTTGHLIYK GGIDKR I++FEKEA +M   SFKYA VLD+L AERER ITIDI LWKFE+ +YY T
Sbjct:    1 HVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVT 74

HSP 2 Score: 28.4906 bits (62), Expect = 1.285e-27
Identity = 13/34 (38.24%), Postives = 21/34 (61.76%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSG 443
            ++APG R + KNMITG S    +  C+ ++  +G
Sbjct:   76 IDAPGHRDFIKNMITGTS----QADCAVLIVAAG 105
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_ADIBE (Elongation factor 1-alpha (Fragment) OS=Adisura bella PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.285e-27
Identity = 56/74 (75.68%), Postives = 62/74 (83.78%), Query Frame = 2 
Query:  116 HVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            HVDSGKSTTTGHLIYK GGIDKR I++FEKEA +M   SFKYA VLD+L AERER ITIDI LWKFE+ +YY T
Sbjct:    1 HVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVT 74

HSP 2 Score: 28.4906 bits (62), Expect = 1.285e-27
Identity = 13/34 (38.24%), Postives = 21/34 (61.76%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNSTD*LEHPCSFILTFSG 443
            ++APG R + KNMITG S    +  C+ ++  +G
Sbjct:   76 IDAPGHRDFIKNMITGTS----QADCAVLIVAAG 105
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1AS_PORPU (Elongation factor 1-alpha S OS=Porphyra purpurea GN=TEF-S PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.788e-26
Identity = 54/79 (68.35%), Postives = 64/79 (81.01%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            L+VIGHVD+GKSTTTGHLIYKLGGID R I +FE +A +M   SFKYA VLD+L AERER ITIDI LWKF + ++ +T
Sbjct:   10 LVVIGHVDAGKSTTTGHLIYKLGGIDARTIAKFEADAKEMGKSSFKYAWVLDKLKAERERGITIDIALWKFSTAKFEYT 88

HSP 2 Score: 27.7202 bits (60), Expect = 1.788e-26
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNS 395
            ++APG R + KNMITG S
Sbjct:   90 IDAPGHRDFIKNMITGTS 107
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A1_XENLA (Elongation factor 1-alpha OS=Xenopus laevis PE=1 SV=2)

HSP 1 Score: 101.679 bits (252), Expect = 4.848e-23
Identity = 46/76 (60.53%), Postives = 59/76 (77.63%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEY 328
            +++IGHVDSGKSTTTGHLIYK GG D R +++ E  AA +   SFK+A +LD+L AERER ITIDI LWKF++  +
Sbjct:   13 IVIIGHVDSGKSTTTGHLIYKCGGFDPRALEKVEAAAAQLGKSSFKFAWILDKLKAERERGITIDISLWKFQTNRF 88

HSP 2 Score: 27.7202 bits (60), Expect = 4.848e-23
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNS 395
            ++APG R + KNMITG S
Sbjct:   93 IDAPGHRDFIKNMITGTS 110
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_BLAHO (Elongation factor 1-alpha OS=Blastocystis hominis PE=2 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.391e-22
Identity = 50/79 (63.29%), Postives = 61/79 (77.22%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            L+VIGHV +GKSTTTGHLIY  GGIDKR I+RFE+    +   SFKYA VL ++ AERER ITIDI LWKFE+ + ++T
Sbjct:   10 LVVIGHVVAGKSTTTGHLIYACGGIDKRTIERFEEGGQRIGKGSFKYAWVLAKMKAERERGITIDISLWKFETRKDFFT 88

HSP 2 Score: 27.7202 bits (60), Expect = 1.391e-22
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNS 395
            ++APG R + KNMITG S
Sbjct:   90 IDAPGHRDFIKNMITGTS 107
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A2_EUPCR (Elongation factor 1-alpha 2 OS=Euplotes crassus GN=EFA2 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.063e-22
Identity = 48/79 (60.76%), Postives = 62/79 (78.48%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            L+VIGHVDSGKSTTTGHLIYKLGGID+R + + E++A ++N  SFKYA VLD L AE+ER ITI+  L +F++    +T
Sbjct:   11 LVVIGHVDSGKSTTTGHLIYKLGGIDERTLAKLEEKALELNKASFKYAFVLDNLKAEQERGITINCALRQFDTPSRSYT 89

HSP 2 Score: 27.7202 bits (60), Expect = 3.063e-22
Identity = 12/25 (48.00%), Postives = 17/25 (68.00%), Query Frame = 3 
Query:  321 PSTTGRGVEAPGRRQWRKNMITGNS 395
            PS +   ++APG + + KNMITG S
Sbjct:   84 PSRSYTIIDAPGHKDFIKNMITGTS 108
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_IGNH4 (Elongation factor 1-alpha OS=Ignicoccus hospitalis (strain KIN4/I / DSM 18386 / JCM 14125) GN=tuf PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.217e-20
Identity = 43/79 (54.43%), Postives = 58/79 (73.42%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            L+VIGHVD GKST  GHL+Y+LG +D++ +K  E+EA      SFKYA +LD+L  ERER +TID+   KFE+ +YY+T
Sbjct:   11 LIVIGHVDHGKSTLVGHLLYELGFVDEKTLKMLEEEAKKRGKESFKYAWLLDKLKEERERGVTIDLTFMKFETPKYYFT 89

HSP 2 Score: 26.9498 bits (58), Expect = 1.217e-20
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNS 395
            ++APG R + KNMITG S
Sbjct:   91 IDAPGHRDFIKNMITGAS 108
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_HYPBU (Elongation factor 1-alpha OS=Hyperthermus butylicus (strain DSM 5456 / JCM 9403) GN=tuf PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.217e-20
Identity = 44/79 (55.70%), Postives = 57/79 (72.15%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            L+VIGHVD GKST  GHL+Y+LG +D++ IK  E+EA      SFKYA +LD L  ERER +TID+   KFE+ +YY+T
Sbjct:   10 LVVIGHVDHGKSTLVGHLLYRLGFVDEKTIKMLEEEAKKKGKESFKYAWLLDRLKEERERGVTIDLTFVKFETKKYYFT 88

HSP 2 Score: 26.9498 bits (58), Expect = 1.217e-20
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNS 395
            ++APG R + KNMITG S
Sbjct:   90 IDAPGHRDFVKNMITGAS 107
[Close]
BLAST of EY720600 vs. ExPASy Swiss-Prot
Match: EF1A_THEPD (Elongation factor 1-alpha OS=Thermofilum pendens (strain Hrk 5) GN=tuf PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.687e-19
Identity = 40/79 (50.63%), Postives = 61/79 (77.22%), Query Frame = 2 
Query:  101 LMVIGHVDSGKSTTTGHLIYKLGGIDKRVIKRFEKEAADMNDRSFKYACVLDELHAERERAITIDIVLWKFESTEYYWT 337
            L+VIGH+D GKST  G L+Y++G +D R+I+++E+EA  M   ++KYA VLD+L  ERE+ ITID+  +KFE+ +Y++T
Sbjct:   10 LVVIGHIDHGKSTLMGRLLYEIGAVDPRLIQQYEEEAKKMGRETWKYAWVLDKLKEEREKGITIDLGFYKFETKKYFFT 88

HSP 2 Score: 26.9498 bits (58), Expect = 1.687e-19
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  342 VEAPGRRQWRKNMITGNS 395
            ++APG R + KNMITG S
Sbjct:   90 IDAPGHRDFVKNMITGAS 107
The following BLAST results are available for this feature:
BLAST of EY720600 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 158
Match NameE-valueIdentityDescription
EF1A_HELAL1.285e-2775.68Elongation factor 1-alpha (Fragment) OS=Heliocheil... [more]
EF1A_ANIIF1.285e-2775.68Elongation factor 1-alpha (Fragment) OS=Anicla inf... [more]
EF1A_ADIBE1.285e-2775.68Elongation factor 1-alpha (Fragment) OS=Adisura be... [more]
EF1AS_PORPU1.788e-2668.35Elongation factor 1-alpha S OS=Porphyra purpurea G... [more]
EF1A1_XENLA4.848e-2360.53Elongation factor 1-alpha OS=Xenopus laevis PE=1 S... [more]
EF1A_BLAHO1.391e-2263.29Elongation factor 1-alpha OS=Blastocystis hominis ... [more]
EF1A2_EUPCR3.063e-2260.76Elongation factor 1-alpha 2 OS=Euplotes crassus GN... [more]
EF1A_IGNH41.217e-2054.43Elongation factor 1-alpha OS=Ignicoccus hospitalis... [more]
EF1A_HYPBU1.217e-2055.70Elongation factor 1-alpha OS=Hyperthermus butylicu... [more]
EF1A_THEPD1.687e-1950.63Elongation factor 1-alpha OS=Thermofilum pendens (... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-703-011-G02-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY720600 ID=EY720600; Name=EY720600; organism=Citrus sinensis; type=EST; length=953bp
CACGCGTCCGGCGGCGAGAGAGAACCATTTTCGCGAATATCATTTTAATA
AGGTTTTTTCTAGATGGGCATGATGGGTAAGGAGAAGACTCACATCAACA
TTGATGGTCATCGGCCATGTCGACTCTGGAAAGTCGACCACCACCGGACA
CTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCAAGAGGTTCG
AAAAGGAAGCTGCTGATATGAACGATAGGTCATTCAAATACGCCTGCGTG
CTCGACGAGCTCCATGCTGAGCGTGAACGTGCTATTACCATTGATATTGT
CTTGTGGAAGTTCGAGAGCACCGAGTACTACTGGACGCGGCGTTGAGGCT
CCTGGACGTCGGCAGTGGAGGAAGAACATGATTACCGGTAACTCAACAGA
CTGACTGGAACATCCTTGTTCGTTTATTCTTACTTTCAGTGGTGGGGAAG
CGGGGGACAGGGAGGGATGGAGGCGAGCGTGGAGGGTGTGGGTTGGCGCG
TCGGTGATGGGGGGGAGCGTGTCGTTGCGCGGGGGGGGGGCAGGGTGGGG
GCCCGCGGAGGTGGGCGGGGGTGGGGGGGCGGAGCGGTGGCGGGCCGGGC
GGGGGCGGATGCCGGGCGGGGGGGCGGGGGTCCTGGGGGGGCGGGGGAGG
AGGGGGGGTGGGTGGGGGAGGGAGCGCGGGGGGGGGGGGGGTGGCGGTGG
GCGGGGGAGGGTGGTGGGGGAGAGTGGGGGGCGGGCGGTGGCGGAGGCAC
GGAGAGGGGGAGAGGGACGGTGAGGCGAGGTGGGTTTGAGTGGGAGGGCC
GCGTGGCGGGGGAGGGTTCGCAGGGGGGGCGGTGGGTGGGAGAGGGGGGG
TTGCGTTGAGAGGGAGGAGGGTGGAGGGAGGGGGGTGGGGGGGATGGGGG
TGAGGGGGGCGAGGCGGGGAGGCGGCGGGGGGTGGGGTGCGGGGGGGCGG
GGG
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