EY720529

Overview
NameEY720529
Unique NameEY720529
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length894
Libraries
Library NameType
Sweet orange fruit, development stadium (4 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY720529 vs. ExPASy Swiss-Prot
Match: PCKA_DEIGD (Phosphoenolpyruvate carboxykinase [ATP] OS=Deinococcus geothermalis (strain DSM 11300) GN=pckA PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 5.776e-48
Identity = 96/198 (48.48%), Postives = 127/198 (64.14%), Query Frame = 2 
Query:   17 YLECHQFGAVLENVVCDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWARHTGWSGGSYGCGSRIKLPYTRKSLDAIQSGSLVECNYKKLMYLGVEIPPESRGVPSQV 610
            Y     +G VLENVV        D +D S+TENTR+AYPI  I N      G HPKN++ L  DAFGVLPP+S+L   QTMY FISG+TA + GTE G+ EP  TFS CFGA F+  HP +YA +LA+K+++ GA  W  +TGW+GG YG G R+ + +TR  ++A  SG L    +++  + G+EIP E  GVP +V
Sbjct:  284 YRTTQTYGTVLENVVLKPDGTP-DLNDGSLTENTRSAYPITQIDNIVPEGRGGHPKNIVFLTADAFGVLPPLSRLTPEQTMYQFISGFTAKIPGTEQGVTEPQPTFSTCFGAPFMPRHPGEYARLLAQKVRESGANVWLVNTGWTGGQYGEGQRMSIQHTRTLINAALSGQLDNVKFEREPFFGLEIPTEVPGVPKEV 480
[Close]
BLAST of EY720529 vs. ExPASy Swiss-Prot
Match: PCKA_BACA2 (Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillus amyloliquefaciens (strain FZB42) GN=pckA PE=3 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 5.776e-48
Identity = 95/193 (49.22%), Postives = 120/193 (62.18%), Query Frame = 2 
Query:   32 QFGAVLENVVCDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWARHTGWSGGSYGCGSRIKLPYTRKSLDAIQSGSLVECNYKKLMYLGVEIPPESRGVPSQV 610
            +FG+VLENVV DE T E DY +   TENTRAAYPIE I N   P +  HP+ ++ L  DAFGVLPP+SKL   Q MYHF+SGYT+ +AGTE G+  P  TFS CFG+ F+ L    YA ML  K+ +HG   +  +TGW+GG YG G R+KL YTR  + A   G L + +       G+  P    GVP +V
Sbjct:  290 RFGSVLENVVLDEKTGEADYDNSFYTENTRAAYPIEMIGNIVQPSIAGHPQAIVFLTADAFGVLPPISKLTKEQAMYHFLSGYTSKLAGTERGVTSPQTTFSTCFGSPFLPLPAHVYAEMLGHKIDEHGVQVFLVNTGWTGGGYGVGERMKLSYTRAMVKAAIEGRLNQTDMTADSIFGLRSPVHVPGVPDEV 482
[Close]
BLAST of EY720529 vs. ExPASy Swiss-Prot
Match: PCKA_STAHJ (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus haemolyticus (strain JCSC1435) GN=pckA PE=3 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 7.544e-48
Identity = 93/193 (48.19%), Postives = 120/193 (62.18%), Query Frame = 2 
Query:   32 QFGAVLENVVCDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWARHTGWSGGSYGCGSRIKLPYTRKSLDAIQSGSLVECNYKKLMYLGVEIPPESRGVPSQV 610
            Q+G +LEN V +E    VD+ D S TENTRAAYPI +I N  +P    HP  +I L  DAFGVLPP++KL  +Q MYHF+SG+T+ +AGTE G+ EP  +FS CFGA F+ L+P KYA +L E + KH    +  +TGW+GG YG G RI L YTR  +D    G L    + K    G+ IP E   VP  +
Sbjct:  293 QYGTILENTVVNERG-VVDFDDNSYTENTRAAYPIHHIDNIVLPSKAAHPNTIIFLTADAFGVLPPIAKLTKSQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFLPLNPNKYADLLGELIDKHDVDVYLVNTGWTGGKYGVGRRISLHYTRYMVDQAIKGKLKNAEFTKDEKFGLSIPTEMEDVPKTI 484
[Close]
BLAST of EY720529 vs. ExPASy Swiss-Prot
Match: PCKA_STAAU (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus GN=pckA PE=3 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 9.853e-48
Identity = 94/193 (48.70%), Postives = 119/193 (61.66%), Query Frame = 2 
Query:   32 QFGAVLENVVCDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWARHTGWSGGSYGCGSRIKLPYTRKSLDAIQSGSLVECNYKKLMYLGVEIPPESRGVPSQV 610
            ++GA+LEN V  E    VD+ D   TENTRAAYPI +I N  +P    HP  +I L  DAFGV+PP+SKLN  Q MYHF+SG+T+ +AGTE G+ EP  +FS CFGA F  LHPT YA +L E +  H    +  +TGW+GG YG G RI L YTR+ ++   SG L    Y K    G+ IP E   VP  +
Sbjct:  293 KYGAILENTVVAEDG-SVDFEDNRYTENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTI 484
[Close]
BLAST of EY720529 vs. ExPASy Swiss-Prot
Match: PCKA_STAAT (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain USA300 / TCH1516) GN=pckA PE=3 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 9.853e-48
Identity = 94/193 (48.70%), Postives = 119/193 (61.66%), Query Frame = 2 
Query:   32 QFGAVLENVVCDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWARHTGWSGGSYGCGSRIKLPYTRKSLDAIQSGSLVECNYKKLMYLGVEIPPESRGVPSQV 610
            ++GA+LEN V  E    VD+ D   TENTRAAYPI +I N  +P    HP  +I L  DAFGV+PP+SKLN  Q MYHF+SG+T+ +AGTE G+ EP  +FS CFGA F  LHPT YA +L E +  H    +  +TGW+GG YG G RI L YTR+ ++   SG L    Y K    G+ IP E   VP  +
Sbjct:  293 KYGAILENTVVAEDG-SVDFEDNRYTENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTI 484
[Close]
BLAST of EY720529 vs. ExPASy Swiss-Prot
Match: PCKA_STAAR (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain MRSA252) GN=pckA PE=3 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 9.853e-48
Identity = 94/193 (48.70%), Postives = 119/193 (61.66%), Query Frame = 2 
Query:   32 QFGAVLENVVCDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWARHTGWSGGSYGCGSRIKLPYTRKSLDAIQSGSLVECNYKKLMYLGVEIPPESRGVPSQV 610
            ++GA+LEN V  E    VD+ D   TENTRAAYPI +I N  +P    HP  +I L  DAFGV+PP+SKLN  Q MYHF+SG+T+ +AGTE G+ EP  +FS CFGA F  LHPT YA +L E +  H    +  +TGW+GG YG G RI L YTR+ ++   SG L    Y K    G+ IP E   VP  +
Sbjct:  293 KYGAILENTVVAEDG-SVDFEDNRYTENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTI 484
[Close]
BLAST of EY720529 vs. ExPASy Swiss-Prot
Match: PCKA_STAAN (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain N315) GN=pckA PE=1 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 9.853e-48
Identity = 94/193 (48.70%), Postives = 119/193 (61.66%), Query Frame = 2 
Query:   32 QFGAVLENVVCDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWARHTGWSGGSYGCGSRIKLPYTRKSLDAIQSGSLVECNYKKLMYLGVEIPPESRGVPSQV 610
            ++GA+LEN V  E    VD+ D   TENTRAAYPI +I N  +P    HP  +I L  DAFGV+PP+SKLN  Q MYHF+SG+T+ +AGTE G+ EP  +FS CFGA F  LHPT YA +L E +  H    +  +TGW+GG YG G RI L YTR+ ++   SG L    Y K    G+ IP E   VP  +
Sbjct:  293 KYGAILENTVVAEDG-SVDFEDNRYTENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTI 484
[Close]
BLAST of EY720529 vs. ExPASy Swiss-Prot
Match: PCKA_STAAM (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=pckA PE=1 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 9.853e-48
Identity = 94/193 (48.70%), Postives = 119/193 (61.66%), Query Frame = 2 
Query:   32 QFGAVLENVVCDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWARHTGWSGGSYGCGSRIKLPYTRKSLDAIQSGSLVECNYKKLMYLGVEIPPESRGVPSQV 610
            ++GA+LEN V  E    VD+ D   TENTRAAYPI +I N  +P    HP  +I L  DAFGV+PP+SKLN  Q MYHF+SG+T+ +AGTE G+ EP  +FS CFGA F  LHPT YA +L E +  H    +  +TGW+GG YG G RI L YTR+ ++   SG L    Y K    G+ IP E   VP  +
Sbjct:  293 KYGAILENTVVAEDG-SVDFEDNRYTENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTI 484
[Close]
BLAST of EY720529 vs. ExPASy Swiss-Prot
Match: PCKA_STAAE (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain Newman) GN=pckA PE=3 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 9.853e-48
Identity = 94/193 (48.70%), Postives = 119/193 (61.66%), Query Frame = 2 
Query:   32 QFGAVLENVVCDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWARHTGWSGGSYGCGSRIKLPYTRKSLDAIQSGSLVECNYKKLMYLGVEIPPESRGVPSQV 610
            ++GA+LEN V  E    VD+ D   TENTRAAYPI +I N  +P    HP  +I L  DAFGV+PP+SKLN  Q MYHF+SG+T+ +AGTE G+ EP  +FS CFGA F  LHPT YA +L E +  H    +  +TGW+GG YG G RI L YTR+ ++   SG L    Y K    G+ IP E   VP  +
Sbjct:  293 KYGAILENTVVAEDG-SVDFEDNRYTENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTI 484
[Close]
BLAST of EY720529 vs. ExPASy Swiss-Prot
Match: PCKA_STAAC (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain COL) GN=pckA PE=3 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 9.853e-48
Identity = 94/193 (48.70%), Postives = 119/193 (61.66%), Query Frame = 2 
Query:   32 QFGAVLENVVCDEHTREVDYSDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWARHTGWSGGSYGCGSRIKLPYTRKSLDAIQSGSLVECNYKKLMYLGVEIPPESRGVPSQV 610
            ++GA+LEN V  E    VD+ D   TENTRAAYPI +I N  +P    HP  +I L  DAFGV+PP+SKLN  Q MYHF+SG+T+ +AGTE G+ EP  +FS CFGA F  LHPT YA +L E +  H    +  +TGW+GG YG G RI L YTR+ ++   SG L    Y K    G+ IP E   VP  +
Sbjct:  293 KYGAILENTVVAEDG-SVDFEDNRYTENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTI 484
The following BLAST results are available for this feature:
BLAST of EY720529 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 284
Match NameE-valueIdentityDescription
PCKA_DEIGD5.776e-4848.48Phosphoenolpyruvate carboxykinase [ATP] OS=Deinoco... [more]
PCKA_BACA25.776e-4849.22Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillu... [more]
PCKA_STAHJ7.544e-4848.19Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl... [more]
PCKA_STAAU9.853e-4848.70Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl... [more]
PCKA_STAAT9.853e-4848.70Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl... [more]
PCKA_STAAR9.853e-4848.70Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl... [more]
PCKA_STAAN9.853e-4848.70Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl... [more]
PCKA_STAAM9.853e-4848.70Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl... [more]
PCKA_STAAE9.853e-4848.70Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl... [more]
PCKA_STAAC9.853e-4848.70Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-703-010-G01-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY720529 ID=EY720529; Name=EY720529; organism=Citrus sinensis; type=EST; length=894bp
CCGTCCGGGAGCCTGATATCTGGAATGCCATCAGTTTGGCGCTGTGTTGG
AAAATGTTGTGTGTGATGAGCATACCAGAGAGGTGGATTATTCTGACAAA
TCTGTCACAGAGAACACTCGTGCTGCCTACCCCATTGAGTATATCCCCAA
TGCAAAGATACCATGCGTTGGTCCTCATCCCAAGAATGTCATCCTTTTGG
CATGTGATGCTTTTGGTGTACTCCCACCAGTGAGCAAGCTGAACCTGGCA
CAAACAATGTACCATTTCATCAGTGGCTACACTGCTTTGGTAGCTGGAAC
AGAGGATGGTATTAAGGAGCCAACGGCAACATTTTCAGCTTGCTTTGGTG
CAGCATTTATCATGCTGCATCCTACCAAGTATGCAGCAATGCTGGCTGAG
AAGATGCAGAAGCATGGTGCTACAGGATGGGCTCGTCACACTGGATGGTC
AGGTGGAAGCTATGGTTGTGGCAGCCGCATCAAGTTACCGTACACTCGAA
AATCATTGGATGCGATACAGTCTGGCAGCCTGGTGGAATGCAATTACAAG
AAACTGATGTATTTGGGGGTGGAAATCCCGCCAGAGTCCAGGGGAGTGCC
TTCACAAGTCGCGATCGCCAGAAGGTTGGGGACGACAGCAGGCGTGCGAG
GAGACAGGCTTAACGTGAGGGGTTTTTAGGAGGAACTCGCAAATAGCGAC
CCGGCAGAGAATTCCGGGGAACAAAAAGTGGAGAAAAAGACCTGGAGGTC
GTCCTAAGGCTGGGGTGGACGAGGCAAAGAAGCCGGACAACTGCGGGGGC
GGCGCACAAAAGGGGCCCGGACCCCCACCCTGCGCTCGTACCTCATTCGA
GACGCCGATTTTTAGAAAAGTGGTTGATACACCGAGGGGGAGCG
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