EY720507

Overview
NameEY720507
Unique NameEY720507
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length712
Libraries
Library NameType
Sweet orange fruit, development stadium (4 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY720507 vs. ExPASy Swiss-Prot
Match: M4K2_MOUSE (Mitogen-activated protein kinase kinase kinase kinase 2 OS=Mus musculus GN=Map4k2 PE=1 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.448e-24
Identity = 62/160 (38.75%), Postives = 90/160 (56.25%), Query Frame = 2 
Query:    5 RPDHLYIYLEYVHPGSINRYVREHCGDITESIVCNFTRQILNGLAYLHSTNTIHRDIKGANLLVDASGVVKLADFGIGKHLT-GLSYELSLKGSPYWMAPEVMQADIQKDGNHRVALLADIWSLGCTVIEMLTGKPPWSEFEGPQAMFKVLNSTPPIPEM 481
            R D L+I +E+   GS+   +    G + E  +    R+ L GL +LHS   IHRDIKGANLL+   G VKLADFG+   LT  ++   S  G+PYWMAPEV  A +++ G +    L D+W+LG T IE+   +PP       +A+  +  S+   P++
Sbjct:   82 RNDRLWICMEFCGGGSLQE-IYHATGPLEERQIAYVCREALKGLHHLHSQGKIHRDIKGANLLLTLQGDVKLADFGVSGELTASVAKRRSFIGTPYWMAPEV--AAVERKGGYNE--LCDVWALGITAIELGELQPPLFHLHPMRALMLMSKSSFQPPKL 236

HSP 2 Score: 31.187 bits (69), Expect = 2.448e-24
Identity = 13/24 (54.17%), Postives = 18/24 (75.00%), Query Frame = 3 
Query:  504 FLRHCLRRNPVERPSACMLLEHPF 575
            FL+  L +NP +RP+A  LL+HPF
Sbjct:  249 FLKLALTKNPKKRPTAERLLQHPF 272
[Close]
BLAST of EY720507 vs. ExPASy Swiss-Prot
Match: M4K2_HUMAN (Mitogen-activated protein kinase kinase kinase kinase 2 OS=Homo sapiens GN=MAP4K2 PE=1 SV=2)

HSP 1 Score: 102.449 bits (254), Expect = 2.449e-24
Identity = 62/160 (38.75%), Postives = 90/160 (56.25%), Query Frame = 2 
Query:    5 RPDHLYIYLEYVHPGSINRYVREHCGDITESIVCNFTRQILNGLAYLHSTNTIHRDIKGANLLVDASGVVKLADFGIGKHLT-GLSYELSLKGSPYWMAPEVMQADIQKDGNHRVALLADIWSLGCTVIEMLTGKPPWSEFEGPQAMFKVLNSTPPIPEM 481
            R D L+I +E+   GS+   +    G + E  +    R+ L GL +LHS   IHRDIKGANLL+   G VKLADFG+   LT  ++   S  G+PYWMAPEV  A +++ G +    L D+W+LG T IE+   +PP       +A+  +  S+   P++
Sbjct:   82 RNDRLWICMEFCGGGSLQE-IYHATGPLEERQIAYVCREALKGLHHLHSQGKIHRDIKGANLLLTLQGDVKLADFGVSGELTASVAKRRSFIGTPYWMAPEV--AAVERKGGYNE--LCDVWALGITAIELGELQPPLFHLHPMRALMLMSKSSFQPPKL 236

HSP 2 Score: 31.187 bits (69), Expect = 2.449e-24
Identity = 13/24 (54.17%), Postives = 18/24 (75.00%), Query Frame = 3 
Query:  504 FLRHCLRRNPVERPSACMLLEHPF 575
            FL+  L +NP +RP+A  LL+HPF
Sbjct:  249 FLKLALTKNPKKRPTAEKLLQHPF 272
[Close]
BLAST of EY720507 vs. ExPASy Swiss-Prot
Match: FRAY2_DICDI (Serine/threonine-protein kinase fray2 OS=Dictyostelium discoideum GN=fray2 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.175e-24
Identity = 55/169 (32.54%), Postives = 94/169 (55.62%), Query Frame = 2 
Query:   11 DHLYIYLEYVHPGSINRYVR-EHCGDITESIVCNFTRQILNGLAYLHSTNTIHRDIKGANLLVDASGVVKLADFGIGKHL--TGLSYELSLKGSPYWMAPEVMQADIQKDGNHRVALLADIWSLGCTVIEMLTGKPPWSEFEGPQAMFKVLNSTPPIPEMLSSEGEGFS 508
            + L++ ++++  GS +  +R        E ++    ++ L  + Y H T  IHRDIK  N+L+D++G ++L+DFG+   L  TG +   +  G+P WMAPE+M+   Q + ++ V    DIWS G T +E+  GK P++E+   + +   L + PP     S EG+G S
Sbjct:  141 ESLWVIMDFLSAGSCSDIMRFSFPQGFEEHVIATILKEALKAICYFHKTGRIHRDIKSGNILIDSNGNIQLSDFGVSATLIDTGETSRNTFVGTPCWMAPEIME---QVNYDYAV----DIWSFGITALELARGKAPFAEYPPMKVLLLTLQNPPP-----SLEGDGES 297

HSP 2 Score: 34.2686 bits (77), Expect = 3.175e-24
Identity = 14/35 (40.00%), Postives = 21/35 (60.00%), Query Frame = 3 
Query:  498 KDFLRHCLRRNPVERPSACMLLEHPFIRNANYQNY 602
            KD +  CL+++P +RP    LLEH F + A   +Y
Sbjct:  304 KDLVEKCLQKDPSKRPLPSKLLEHRFFKQAKKPDY 338
[Close]
BLAST of EY720507 vs. ExPASy Swiss-Prot
Match: PLK4_AEDAE (Serine/threonine-protein kinase PLK4 OS=Aedes aegypti GN=SAK PE=3 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 3.190e-24
Identity = 58/161 (36.02%), Postives = 88/161 (54.66%), Query Frame = 2 
Query:   14 HLYIYLEYVHPGSINRYVREHCGDITESIVCNFTRQILNGLAYLHSTNTIHRDIKGANLLVDASGVVKLADFGIGKHLTGLSYE-LSLKGSPYWMAPEVMQADIQKDGNHRVALLADIWSLGCTVIEMLTGKPPWSEFEGPQAMFKVLNSTPPIPEMLSSE 493
            H+Y+ LE    G + RY+RE      E    +  +Q+++GL YLHS + +HRD+  ANLL+     VK++DFG+   LT    + ++L G+P +++PEV         +H   L AD+W LGC +   L GKPP+        + KV+ S   IP  +SSE
Sbjct:   82 HVYLVLELAENGELQRYLRETKKTFNEYEAASVLKQVVDGLLYLHSHHILHRDMSLANLLLTKQMTVKISDFGLATQLTRPDEKHMTLCGTPNYISPEVASR-----ASH--GLPADVWGLGCMLYTFLVGKPPFDTEGVKSTLTKVVMSNYTIPPYISSE 235

HSP 2 Score: 32.7278 bits (73), Expect = 3.190e-24
Identity = 14/36 (38.89%), Postives = 23/36 (63.89%), Query Frame = 3 
Query:  498 KDFLRHCLRRNPVERPSACMLLEHPFIRNA-NYQNY 602
            +D +   L++NP ER     +L+HPF+R A +Y +Y
Sbjct:  237 RDLIDQLLKKNPAERIKLDQVLQHPFMRKATSYDSY 272
[Close]
BLAST of EY720507 vs. ExPASy Swiss-Prot
Match: NRK_HUMAN (Nik-related protein kinase OS=Homo sapiens GN=NRK PE=2 SV=2)

HSP 1 Score: 102.064 bits (253), Expect = 4.104e-24
Identity = 57/153 (37.25%), Postives = 84/153 (54.90%), Query Frame = 2 
Query:   17 LYIYLEYVHPGSINRYVREHCGD-ITESIVCNFTRQILNGLAYLHSTNTIHRDIKGANLLVDASGVVKLADFGIGKHLTGLS-YELSLKGSPYWMAPEVMQADIQKDGNHRVALLADIWSLGCTVIEMLTGKPPWSEFEGPQAMFKVLNSTPP 469
            L++ +E    GS+   VR      + E  +    R+IL GLA+LH+   IHRDIKG N+L+  +  VKL DFG+   ++  +    S  G+PYWMAPEV+  D  +D        +D+WS+G T IEM  G PP    +  +A+F +L  + P
Sbjct:  125 LWMVMELCAAGSVTDVVRMTSNQSLKEDWIAYICREILQGLAHLHAHRVIHRDIKGQNVLLTHNAEVKLVDFGVSAQVSRTNGRRNSFIGTPYWMAPEVIDCD--EDPRRSYDYRSDVWSVGITAIEMAEGAPPLCNLQPLEALFVILRESAP 275

HSP 2 Score: 30.8018 bits (68), Expect = 4.104e-24
Identity = 12/49 (24.49%), Postives = 28/49 (57.14%), Query Frame = 3 
Query:  456 TAPHLYQKCYLPRGKDFLRHCLRRNPVERPSACMLLEHPFIRNANYQNY 602
            +AP +    +  +  +F+  C  +N + RP++  +L+HPF+R+   + +
Sbjct:  273 SAPTVKSSGWSRKFHNFMEKCTIKNFLFRPTSANMLQHPFVRDIKNERH 321
[Close]
BLAST of EY720507 vs. ExPASy Swiss-Prot
Match: M3K1_HUMAN (Mitogen-activated protein kinase kinase kinase 1 OS=Homo sapiens GN=MAP3K1 PE=1 SV=4)

HSP 1 Score: 105.916 bits (263), Expect = 4.107e-24
Identity = 64/166 (38.55%), Postives = 100/166 (60.24%), Query Frame = 2 
Query:   23 IYLEYVHPGSINRYVREHCGDITESIVCNFTRQILNGLAYLHSTNTIHRDIKGANLLVDASGV-VKLADFGIGKHL----TGL-SYELSLKGSPYWMAPEVMQADIQKDGNHRVALLADIWSLGCTVIEMLTGKPPWSEFEGPQ---AMFKVLNST--PPIPEMLS 487
            +++E++  GS+   + ++ G   ES+V N+T Q+L GL+YLH    IHRD+KGANLL+D++G  +++ADFG    L    TG   ++  L G+  +MAPEV++   Q+ G        D+WS+GC +IEM   KPPW+  +       +FK+ ++T  P IP  LS
Sbjct: 1321 LFIEWMAGGSVAHLLSKY-GAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRG--QQYGRS-----CDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLS 1478

HSP 2 Score: 26.9498 bits (58), Expect = 4.107e-24
Identity = 12/31 (38.71%), Postives = 16/31 (51.61%), Query Frame = 3 
Query:  489 PRGKDFLRHCLRRNPVERPSACMLLEHPFIR 581
            P  +D    CL   P +RP +  LL+HP  R
Sbjct: 1479 PGLRDVALRCLELQPQDRPPSRELLKHPVFR 1509
[Close]
BLAST of EY720507 vs. ExPASy Swiss-Prot
Match: PAKM_DROME (Serine/threonine-protein kinase PAK mbt OS=Drosophila melanogaster GN=mbt PE=1 SV=2)

HSP 1 Score: 98.2117 bits (243), Expect = 4.163e-24
Identity = 60/154 (38.96%), Postives = 83/154 (53.90%), Query Frame = 2 
Query:   11 DHLYIYLEYVHPGSINRYVREHCGDITESIVCNFTRQILNGLAYLHSTNTIHRDIKGANLLVDASGVVKLADFGIGKHLT-GLSYELSLKGSPYWMAPEVMQADIQKDGNHRVALLADIWSLGCTVIEMLTGKPPWSEFEGPQAMFKVLNSTPP 469
            D L++ +EY+  G++   V     D  E  +    +Q L  LAYLHS   IHRDIK  ++L+ A G VKL+DFG    ++  L    SL G+PYWM+PEV+                DIWSLG  VIEM+ G+PP+      QAM ++ +  PP
Sbjct:  436 DELWVVMEYLEGGALTDIVTHSRMD--EEQIATVCKQCLKALAYLHSQGVIHRDIKSDSILLAADGRVKLSDFGFCAQVSQELPKRKSLVGTPYWMSPEVI-------SRLPYGPEVDIWSLGIMVIEMVDGEPPFFNEPPLQAMRRIRDMQPP 580

HSP 2 Score: 34.6538 bits (78), Expect = 4.163e-24
Identity = 16/33 (48.48%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:  489 PRGKDFLRHCLRRNPVERPSACMLLEHPFIRNA 587
            PR + FL   L R+P +R +A  LL HPF+R A
Sbjct:  590 PRLQSFLDRMLVRDPAQRATAAELLAHPFLRQA 622
[Close]
BLAST of EY720507 vs. ExPASy Swiss-Prot
Match: PAK2_RAT (Serine/threonine-protein kinase PAK 2 OS=Rattus norvegicus GN=Pak2 PE=1 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 4.177e-24
Identity = 64/163 (39.26%), Postives = 91/163 (55.83%), Query Frame = 2 
Query:   11 DHLYIYLEYVHPGSINRYVREHCGDITESIVCNFTRQILNGLAYLHSTNTIHRDIKGANLLVDASGVVKLADFGIGKHLT-GLSYELSLKGSPYWMAPEVMQADIQKDGNHRVALLADIWSLGCTVIEMLTGKPPWSEFEGPQAMFKV-LNSTPPI--PEMLS 487
            D L++ +EY+  GS+   V E C D  E+ +    R+ L  L +LH+   IHRDIK  N+L+   G VKL DFG    +T   S   ++ G+PYWMAPEV+    +K    +V    DIWSLG   IEM+ G+PP+      +A++ +  N TP +  PE LS
Sbjct:  317 DELFVVMEYLAGGSLTDVVTETCMD--EAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVV---TRKAYGPKV----DIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLS 470

HSP 2 Score: 31.9574 bits (71), Expect = 4.177e-24
Identity = 15/33 (45.45%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:  489 PRGKDFLRHCLRRNPVERPSACMLLEHPFIRNA 587
            P  +DFL  CL  +  +R SA  LL+HPF++ A
Sbjct:  471 PIFRDFLNRCLEMDVEKRGSAKELLQHPFLKLA 503
[Close]
BLAST of EY720507 vs. ExPASy Swiss-Prot
Match: PAK2_RABIT (Serine/threonine-protein kinase PAK 2 OS=Oryctolagus cuniculus GN=PAK2 PE=2 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 4.177e-24
Identity = 64/163 (39.26%), Postives = 91/163 (55.83%), Query Frame = 2 
Query:   11 DHLYIYLEYVHPGSINRYVREHCGDITESIVCNFTRQILNGLAYLHSTNTIHRDIKGANLLVDASGVVKLADFGIGKHLT-GLSYELSLKGSPYWMAPEVMQADIQKDGNHRVALLADIWSLGCTVIEMLTGKPPWSEFEGPQAMFKV-LNSTPPI--PEMLS 487
            D L++ +EY+  GS+   V E C D  E+ +    R+ L  L +LH+   IHRDIK  N+L+   G VKL DFG    +T   S   ++ G+PYWMAPEV+    +K    +V    DIWSLG   IEM+ G+PP+      +A++ +  N TP +  PE LS
Sbjct:  317 DELFVVMEYLAGGSLTDVVTETCMD--EAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVV---TRKAYGPKV----DIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLS 470

HSP 2 Score: 31.9574 bits (71), Expect = 4.177e-24
Identity = 15/33 (45.45%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:  489 PRGKDFLRHCLRRNPVERPSACMLLEHPFIRNA 587
            P  +DFL  CL  +  +R SA  LL+HPF++ A
Sbjct:  471 PIFRDFLNRCLEMDVEKRGSAKELLQHPFLKLA 503
[Close]
BLAST of EY720507 vs. ExPASy Swiss-Prot
Match: PAK2_MOUSE (Serine/threonine-protein kinase PAK 2 OS=Mus musculus GN=Pak2 PE=1 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 4.177e-24
Identity = 64/163 (39.26%), Postives = 91/163 (55.83%), Query Frame = 2 
Query:   11 DHLYIYLEYVHPGSINRYVREHCGDITESIVCNFTRQILNGLAYLHSTNTIHRDIKGANLLVDASGVVKLADFGIGKHLT-GLSYELSLKGSPYWMAPEVMQADIQKDGNHRVALLADIWSLGCTVIEMLTGKPPWSEFEGPQAMFKV-LNSTPPI--PEMLS 487
            D L++ +EY+  GS+   V E C D  E+ +    R+ L  L +LH+   IHRDIK  N+L+   G VKL DFG    +T   S   ++ G+PYWMAPEV+    +K    +V    DIWSLG   IEM+ G+PP+      +A++ +  N TP +  PE LS
Sbjct:  317 DELFVVMEYLAGGSLTDVVTETCMD--EAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVV---TRKAYGPKV----DIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLS 470

HSP 2 Score: 31.9574 bits (71), Expect = 4.177e-24
Identity = 15/33 (45.45%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:  489 PRGKDFLRHCLRRNPVERPSACMLLEHPFIRNA 587
            P  +DFL  CL  +  +R SA  LL+HPF++ A
Sbjct:  471 PIFRDFLNRCLEMDVEKRGSAKELLQHPFLKLA 503
The following BLAST results are available for this feature:
BLAST of EY720507 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
M4K2_MOUSE2.448e-2438.75Mitogen-activated protein kinase kinase kinase kin... [more]
M4K2_HUMAN2.449e-2438.75Mitogen-activated protein kinase kinase kinase kin... [more]
FRAY2_DICDI3.175e-2432.54Serine/threonine-protein kinase fray2 OS=Dictyoste... [more]
PLK4_AEDAE3.190e-2436.02Serine/threonine-protein kinase PLK4 OS=Aedes aegy... [more]
NRK_HUMAN4.104e-2437.25Nik-related protein kinase OS=Homo sapiens GN=NRK ... [more]
M3K1_HUMAN4.107e-2438.55Mitogen-activated protein kinase kinase kinase 1 O... [more]
PAKM_DROME4.163e-2438.96Serine/threonine-protein kinase PAK mbt OS=Drosoph... [more]
PAK2_RAT4.177e-2439.26Serine/threonine-protein kinase PAK 2 OS=Rattus no... [more]
PAK2_RABIT4.177e-2439.26Serine/threonine-protein kinase PAK 2 OS=Oryctolag... [more]
PAK2_MOUSE4.177e-2439.26Serine/threonine-protein kinase PAK 2 OS=Mus muscu... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-703-010-D11-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY720507 ID=EY720507; Name=EY720507; organism=Citrus sinensis; type=EST; length=712bp
CACGCGTCCGGATCACCTCTATATATATCTGGAATATGTCCATCCTGGCT
CAATTAATAGATACGTCCGTGAACATTGCGGAGATATTACAGAAAGTATA
GTTTGCAATTTCACCCGTCAAATTCTCAATGGGTTGGCCTACCTGCATAG
CACCAACACCATTCACAGGGATATTAAAGGTGCAAATTTGCTAGTTGATG
CATCTGGTGTTGTTAAGCTTGCAGATTTTGGGATAGGAAAACATCTTACA
GGACTCTCCTATGAATTGTCACTGAAGGGCAGCCCATATTGGATGGCTCC
AGAGGTCATGCAGGCTGATATTCAAAAGGATGGAAATCATAGGGTTGCTT
TACTTGCTGATATATGGAGCTTAGGTTGTACTGTCATTGAGATGCTTACT
GGTAAACCTCCTTGGAGTGAGTTTGAAGGGCCTCAAGCTATGTTCAAGGT
CTTGAACAGCACCCCACCTATACCAGAAATGTTATCTTCCGAGGGGGAAG
GATTTTCTTCGACATTGCCTTCGCAGAAATCCTGTAGAGCGGCCATCAGC
TTGTATGCTACTGGAGCATCCTTTTATACGAAATGCAAATTATCAAAATT
ATTAGAAGCTCAAGTGAATCAAACTTTGTATGTACGTACCTTAGNTAAGA
GCTACTCCATTACAGTATATTGAATGGGAAGCTAACTACTTTATTTTAAA
AAAAAACAAATA
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