EY720383 | Citrus Genome Database

Overview


Name	EY720383
Unique Name	EY720383
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	871

Libraries


Library Name	Type
Sweet orange fruit, development stadium (4 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY720383 vs. ExPASy Swiss-Prot
Match: UBIQ_LEIMA (Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.958e-33
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = 1

Query:  202 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 429
            MQIFVKTLTGKTI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 67.781 bits (164), Expect = 1.975e-21
Identity = 33/38 (86.84%), Postives = 35/38 (92.11%), Query Frame = 1

Query:  430 MQIFVKTLTGKTITLEVDSSDTIDNVKAKIQHKEGIPP 543
            MQIFVKTLTGKTI LEV+ SDTI+NVKAKIQ KEGIPP
Sbjct:    1 MQIFVKTLTGKTIALEVEPSDTIENVKAKIQDKEGIPP 38

HSP 3 Score: 56.225 bits (134), Expect = 1.975e-21
Identity = 26/30 (86.67%), Postives = 28/30 (93.33%), Query Frame = 3

Query:  570 GKALEEGRTLADYNIQKESALHLVLRVRGG 659
            GK LEEGRTL+DYNIQKES LHLVLR+RGG
Sbjct:   47 GKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 4 Score: 96.6709 bits (239), Expect = 1.861e-19
Identity = 47/53 (88.68%), Postives = 50/53 (94.34%), Query Frame = 1

Query:   43 DNVKAKIQDKEGILPDHHRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 201
            +NVKAKIQDKEGI PD  RLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:   24 ENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of EY720383 vs. ExPASy Swiss-Prot
Match: UBIQ_LEITA (Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 9.516e-32
Identity = 69/76 (90.79%), Postives = 72/76 (94.74%), Query Frame = 1

Query:  202 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 429
            MQIFVKTLTG TI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGTTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 65.4698 bits (158), Expect = 4.650e-20
Identity = 32/38 (84.21%), Postives = 34/38 (89.47%), Query Frame = 1

Query:  430 MQIFVKTLTGKTITLEVDSSDTIDNVKAKIQHKEGIPP 543
            MQIFVKTLTG TI LEV+ SDTI+NVKAKIQ KEGIPP
Sbjct:    1 MQIFVKTLTGTTIALEVEPSDTIENVKAKIQDKEGIPP 38

HSP 3 Score: 53.9138 bits (128), Expect = 4.650e-20
Identity = 25/29 (86.21%), Postives = 27/29 (93.10%), Query Frame = 3

Query:  573 KALEEGRTLADYNIQKESALHLVLRVRGG 659
            K LEEGRTL+DYNIQKES LHLVLR+RGG
Sbjct:   48 KQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 4 Score: 93.9745 bits (232), Expect = 1.207e-18
Identity = 46/53 (86.79%), Postives = 49/53 (92.45%), Query Frame = 1

Query:   43 DNVKAKIQDKEGILPDHHRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 201
            +NVKAKIQDKEGI PD  RLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:   24 ENVKAKIQDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of EY720383 vs. ExPASy Swiss-Prot
Match: UBIL_NPVOP (Ubiquitin-like protein OS=Orgyia pseudotsugata multicapsid polyhedrosis virus GN=V-UBI PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 4.887e-28
Identity = 61/76 (80.26%), Postives = 67/76 (88.16%), Query Frame = 1

Query:  202 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 429
            MQIFVKTLTGKTIT+E E  DT+  VK KI DKEG+P DQQRLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFVKTLTGKTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGG 76

HSP 2 Score: 54.299 bits (129), Expect = 4.601e-16
Identity = 25/37 (67.57%), Postives = 29/37 (78.38%), Query Frame = 1

Query:  430 MQIFVKTLTGKTITLEVDSSDTIDNVKAKIQHKEGIP 540
            MQIFVKTLTGKTIT+E +  DT+  VK KI  KEG+P
Sbjct:    1 MQIFVKTLTGKTITVETEPGDTVGQVKQKIADKEGVP 37

HSP 3 Score: 51.6026 bits (122), Expect = 4.601e-16
Identity = 23/30 (76.67%), Postives = 27/30 (90.00%), Query Frame = 3

Query:  570 GKALEEGRTLADYNIQKESALHLVLRVRGG 659
            GK LE+ +TLADYNIQKES LH+VLR+RGG
Sbjct:   47 GKQLEDAQTLADYNIQKESTLHMVLRLRGG 76

HSP 4 Score: 82.4185 bits (202), Expect = 3.633e-15
Identity = 40/51 (78.43%), Postives = 44/51 (86.27%), Query Frame = 1

Query:   49 VKAKIQDKEGILPDHHRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 201
            VK KI DKEG+  D  RLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG
Sbjct:   26 VKQKIADKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGG 76

[Close]

BLAST of EY720383 vs. ExPASy Swiss-Prot
Match: UBIL_NPVAC (Ubiquitin-like protein OS=Autographa californica nuclear polyhedrosis virus GN=V-UBI PE=3 SV=2)

HSP 1 Score: 122.479 bits (306), Expect = 3.168e-27
Identity = 58/76 (76.32%), Postives = 68/76 (89.47%), Query Frame = 1

Query:  202 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 429
            MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76

HSP 2 Score: 82.4185 bits (202), Expect = 3.633e-15
Identity = 39/52 (75.00%), Postives = 45/52 (86.54%), Query Frame = 1

Query:   46 NVKAKIQDKEGILPDHHRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 201
            ++K KI DKEG+  D  RLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG
Sbjct:   25 DLKQKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76

HSP 3 Score: 51.2174 bits (121), Expect = 6.346e-15
Identity = 22/30 (73.33%), Postives = 27/30 (90.00%), Query Frame = 3

Query:  570 GKALEEGRTLADYNIQKESALHLVLRVRGG 659
            GK LE+ +T+ADYNIQKES LH+VLR+RGG
Sbjct:   47 GKQLEDSKTMADYNIQKESTLHMVLRLRGG 76

HSP 4 Score: 50.8322 bits (120), Expect = 6.346e-15
Identity = 22/37 (59.46%), Postives = 30/37 (81.08%), Query Frame = 1

Query:  430 MQIFVKTLTGKTITLEVDSSDTIDNVKAKIQHKEGIP 540
            MQIF+KTLTGKTIT E + ++T+ ++K KI  KEG+P
Sbjct:    1 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVP 37

[Close]

BLAST of EY720383 vs. ExPASy Swiss-Prot
Match: UBIQ_LUMTE (Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 SV=2)

HSP 1 Score: 120.553 bits (301), Expect = 1.204e-26
Identity = 60/63 (95.24%), Postives = 62/63 (98.41%), Query Frame = 1

Query:  235 TITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 423
            TITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:    1 TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63

HSP 2 Score: 93.5893 bits (231), Expect = 1.576e-18
Identity = 46/51 (90.20%), Postives = 48/51 (94.12%), Query Frame = 1

Query:   43 DNVKAKIQDKEGILPDHHRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 195
            +NVKAKIQDKEGI PD  RLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:   13 ENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63

HSP 3 Score: 50.447 bits (119), Expect = 3.979e-14
Identity = 23/28 (82.14%), Postives = 26/28 (92.86%), Query Frame = 3

Query:  570 GKALEEGRTLADYNIQKESALHLVLRVR 653
            GK LE+GRTL+DYNIQKES LHLVLR+R
Sbjct:   36 GKQLEDGRTLSDYNIQKESTLHLVLRLR 63

HSP 4 Score: 48.9062 bits (115), Expect = 3.979e-14
Identity = 23/27 (85.19%), Postives = 25/27 (92.59%), Query Frame = 1

Query:  463 TITLEVDSSDTIDNVKAKIQHKEGIPP 543
            TITLEV+ SDTI+NVKAKIQ KEGIPP
Sbjct:    1 TITLEVEPSDTIENVKAKIQDKEGIPP 27

[Close]

BLAST of EY720383 vs. ExPASy Swiss-Prot
Match: RUB1_DESAN (NEDD8-like protein RUB1 OS=Deschampsia antarctica GN=RUB1 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.872e-20
Identity = 48/74 (64.86%), Postives = 59/74 (79.73%), Query Frame = 1

Query:  208 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 429
            I VKTLTGK I +++E +DTID VK ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRVKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of EY720383 vs. ExPASy Swiss-Prot
Match: RUB2_ORYSJ (NEDD8-like protein RUB2 OS=Oryza sativa subsp. japonica GN=RUB2 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.751e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 1

Query:  208 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 429
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of EY720383 vs. ExPASy Swiss-Prot
Match: RUB1_ORYSJ (NEDD8-like protein RUB1 OS=Oryza sativa subsp. japonica GN=RUB1 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.751e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 1

Query:  208 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 429
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of EY720383 vs. ExPASy Swiss-Prot
Match: RUB1_ARATH (NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN=RUB1 PE=1 SV=2)

HSP 1 Score: 98.9821 bits (245), Expect = 3.751e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 1

Query:  208 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 429
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of EY720383 vs. ExPASy Swiss-Prot
Match: NEDD8_DROME (NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.751e-20
Identity = 46/79 (58.23%), Postives = 60/79 (75.95%), Query Frame = 1

Query:  202 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQI 438
            M I VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79

HSP 2 Score: 68.1662 bits (165), Expect = 7.090e-11
Identity = 31/56 (55.36%), Postives = 40/56 (71.43%), Query Frame = 1

Query:   43 DNVKAKIQDKEGILPDHHRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQI 210
            D +K ++++KEGI P   RLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:   24 DRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79

The following BLAST results are available for this feature:

BLAST of EY720383 vs. ExPASy Swiss-Prot

BLAST of EY720383 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 94


Match Name	E-value	Identity	Description
UBIQ_LEIMA	2.958e-33	93.42	Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1	[more]
UBIQ_LEITA	9.516e-32	90.79	Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3...	[more]
UBIL_NPVOP	4.887e-28	80.26	Ubiquitin-like protein OS=Orgyia pseudotsugata mul...	[more]
UBIL_NPVAC	3.168e-27	76.32	Ubiquitin-like protein OS=Autographa californica n...	[more]
UBIQ_LUMTE	1.204e-26	95.24	Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 ...	[more]
RUB1_DESAN	2.872e-20	64.86	NEDD8-like protein RUB1 OS=Deschampsia antarctica ...	[more]
RUB2_ORYSJ	3.751e-20	63.51	NEDD8-like protein RUB2 OS=Oryza sativa subsp. jap...	[more]
RUB1_ORYSJ	3.751e-20	63.51	NEDD8-like protein RUB1 OS=Oryza sativa subsp. jap...	[more]
RUB1_ARATH	3.751e-20	63.51	NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN...	[more]
NEDD8_DROME	3.751e-20	58.23	NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-703-008-F10-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY720383 ID=EY720383; Name=EY720383; organism=Citrus sinensis; type=EST; length=871bp
GTGTACCGAGTCCGGAATCCCTGGGNTGACCCACGCCGTCCGGACAACGT
AAAGGCCAAGATCCAAGACAAGGAGGGAATCCTGCCGGACCACCACAGGC
TTATCTTTGCTGGAAAGCAGCTCGAGGACGGCCGCACCTTGGCCGATTAC
AACATCCAGAAGGAGTCAACCCTTCATCTTGTCCTTCGTCTCCGCGGAGG
CATGCAGATCTTCGTGAAAACCCTGACTGGCAAAACTATTACTCTCGAAG
TCGAGAGCTCCGACACCATCGACAACGTCAAGGCGAAGATTCAAGACAAG
GAGGGAATCCCCCCGGACCAGCAGAGGCTTATCTTCGCTGGAAAGCAGCT
CGAGGATGGCCGCACCTTGGCCGATTACAACATACAGAAGGAGTCAACCC
TCCATCTTGTCCTTCGTCTCCGCGGAGGCATGCAGATCTTTGTCAAAACC
CTAACCGGGAAGACGATAACCCTCGAGGTTGACAGCTCAGACACGATCGA
TAACGTTAAGGCGAAGATTCAACACAAGGAGGGAATCCCCCCGGGGCCAT
CAGAGGCTGATCTTTGCTGGGCAAGGCACTGGAAGAAGGGCGCACCTTGG
CTGATTACAATATCCAGAAGGAGTCAGCCCTTCATCTCGTCCTCAGGGTC
CGTGGTGGTATGGAAATCTTCTTGAAAGCCTTACTGGGCAAACCATCACT
CTGGAAGTGGAGACGTCCCATACGATCGATCCCGTCGGGGCCGTATTCAG
GACACGGAGGGATCCCACACATCCGGCAAAGGTCATCTGTGCTCGGAACG
CCTGCCATGGCCCATCCGACGGAGTGGACATGCGGGGGGCTTAGCCGGGC
GGTGTGGGGAGCCTCGCCGTC