EY695515

Overview
NameEY695515
Unique NameEY695515
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length903
Libraries
Library NameType
Sweet orange fruit, development stadium (1 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
EY695515_ssr57 microsatellite EY695515_ssr57:57..71. BLAST: Citrus ESTs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY695515 vs. ExPASy Swiss-Prot
Match: NDK_THETN (Nucleoside diphosphate kinase OS=Thermoanaerobacter tengcongensis GN=ndk PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 1.248e-28
Identity = 57/106 (53.77%), Postives = 72/106 (67.92%), Query Frame = 2 
Query:  341 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGPVIAMVWEGEGVITYGRKLIGATDPQRSEPGTIRGDLA 658
            ME T   +KPDGV+RGLI EI+ R+E KG +L A K+V PS E  +KHY + K +PFF  L E++SSGPV AMV EGE  +   R + GAT  + + PGTIRGD A
Sbjct:    4 MEVTLAIVKPDGVKRGLIGEILKRYENKGLRLKAAKVVTPSIELLEKHYEEHKGKPFFKPLIEYMSSGPVFAMVLEGENAVKLVRMINGATKVEDALPGTIRGDFA 109

HSP 2 Score: 30.0314 bits (66), Expect = 1.248e-28
Identity = 11/19 (57.89%), Postives = 14/19 (73.68%), Query Frame = 1 
Query:  676 IIHGSDGPETRKDEITLWF 732
            ++HGSD  E+ K EI LWF
Sbjct:  116 VVHGSDSVESAKREIALWF 134
[Close]
BLAST of EY695515 vs. ExPASy Swiss-Prot
Match: NDK_CORGL (Nucleoside diphosphate kinase OS=Corynebacterium glutamicum GN=ndk PE=1 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.248e-28
Identity = 58/110 (52.73%), Postives = 75/110 (68.18%), Query Frame = 2 
Query:  344 ERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGPVIAMVWEGEGVITYGRKLIGATDP-QRSEPGTIRGDLAVVVG 670
            ERT I IKPDGV  G + EII+R ERKG KL A+ + V  +E A+KHY +  ++PFF  L EF++S P+IA + EGE  I   R+L G TDP  ++ PGTIRGD A+ VG
Sbjct:    3 ERTLILIKPDGVTNGHVGEIIARIERKGLKLAALDLRVADRETAEKHYEEHADKPFFGELVEFITSAPLIAGIVEGERAIDAWRQLAGGTDPVAKATPGTIRGDFALTVG 112

HSP 2 Score: 31.9574 bits (71), Expect = 1.248e-28
Identity = 10/21 (47.62%), Postives = 17/21 (80.95%), Query Frame = 1 
Query:  670 KRIIHGSDGPETRKDEITLWF 732
            + ++HGSD PE+ + EI++WF
Sbjct:  113 ENVVHGSDSPESAEREISIWF 133
[Close]
BLAST of EY695515 vs. ExPASy Swiss-Prot
Match: NDK_CORGB (Nucleoside diphosphate kinase OS=Corynebacterium glutamicum (strain R) GN=ndk PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.248e-28
Identity = 58/110 (52.73%), Postives = 75/110 (68.18%), Query Frame = 2 
Query:  344 ERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGPVIAMVWEGEGVITYGRKLIGATDP-QRSEPGTIRGDLAVVVG 670
            ERT I IKPDGV  G + EII+R ERKG KL A+ + V  +E A+KHY +  ++PFF  L EF++S P+IA + EGE  I   R+L G TDP  ++ PGTIRGD A+ VG
Sbjct:    3 ERTLILIKPDGVTNGHVGEIIARIERKGLKLAALDLRVADRETAEKHYEEHADKPFFGELVEFITSAPLIAGIVEGERAIDAWRQLAGGTDPVAKATPGTIRGDFALTVG 112

HSP 2 Score: 31.9574 bits (71), Expect = 1.248e-28
Identity = 10/21 (47.62%), Postives = 17/21 (80.95%), Query Frame = 1 
Query:  670 KRIIHGSDGPETRKDEITLWF 732
            + ++HGSD PE+ + EI++WF
Sbjct:  113 ENVVHGSDSPESAEREISIWF 133
[Close]
BLAST of EY695515 vs. ExPASy Swiss-Prot
Match: NDK_PYRKO (Nucleoside diphosphate kinase OS=Pyrococcus kodakaraensis GN=ndk PE=3 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 2.104e-28
Identity = 53/111 (47.75%), Postives = 79/111 (71.17%), Query Frame = 2 
Query:  338 EMERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGPVIAMVWEGEGVITYGRKLIGATDPQRSEPGTIRGDLAVVVG 670
            ++ERT I +KPD V RGL+ EIISRFE++G K+V +K++   ++ A+KHY + K +PFF  L ++++  P + MV EG   I+  RK+ GATDP+ +EPG+IRGD  + VG
Sbjct:    5 QIERTLIILKPDAVVRGLMGEIISRFEKRGLKIVGMKMIWIDRKLAEKHYAEHKGKPFFEPLIDYITKAPSVVMVVEGRYAISVVRKMAGATDPKDAEPGSIRGDYGLDVG 115

HSP 2 Score: 30.8018 bits (68), Expect = 2.104e-28
Identity = 12/22 (54.55%), Postives = 16/22 (72.73%), Query Frame = 1 
Query:  676 IIHGSDGPETRKDEITLWFTPE 741
            +IH SD PE+ + EI L+F PE
Sbjct:  121 VIHASDSPESAEREINLYFKPE 142
[Close]
BLAST of EY695515 vs. ExPASy Swiss-Prot
Match: NDK_DESHY (Nucleoside diphosphate kinase OS=Desulfitobacterium hafniense (strain Y51) GN=ndk PE=3 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 2.111e-28
Identity = 52/111 (46.85%), Postives = 78/111 (70.27%), Query Frame = 2 
Query:  341 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGPVIAMVWEGEGVITYGRKLIGATDPQRSEPGTIRGDLAVVVGR 673
            ME+TFI +KPD VQRGL+ +II+RFE KG KLV +K++   +  A++HY + K + FF    +++ S PV+AMVWEG+ V+   R+L+GAT+P  + PG+IRG   + + R
Sbjct:    1 MEKTFIMLKPDAVQRGLVGQIIARFETKGCKLVGMKLMSVDQALAEQHYAEHKGKSFFEPTVQYIMSSPVVAMVWEGKNVVALARELMGATNPANANPGSIRGSFGMDISR 111

HSP 2 Score: 28.1054 bits (61), Expect = 2.111e-28
Identity = 11/24 (45.83%), Postives = 16/24 (66.67%), Query Frame = 1 
Query:  670 KRIIHGSDGPETRKDEITLWFTPE 741
            + +IHGSD   + + EI L+F PE
Sbjct:  111 RNVIHGSDSVASAEREIALYFRPE 134
[Close]
BLAST of EY695515 vs. ExPASy Swiss-Prot
Match: NDK_DESHD (Nucleoside diphosphate kinase OS=Desulfitobacterium hafniense (strain DCB-2 / DSM 10664) GN=ndk PE=3 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 2.111e-28
Identity = 52/111 (46.85%), Postives = 78/111 (70.27%), Query Frame = 2 
Query:  341 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGPVIAMVWEGEGVITYGRKLIGATDPQRSEPGTIRGDLAVVVGR 673
            ME+TFI +KPD VQRGL+ +II+RFE KG KLV +K++   +  A++HY + K + FF    +++ S PV+AMVWEG+ V+   R+L+GAT+P  + PG+IRG   + + R
Sbjct:    1 MEKTFIMLKPDAVQRGLVGQIIARFEAKGCKLVGMKLMSVDQALAEQHYAEHKGKSFFEPTVQYIMSSPVVAMVWEGKNVVALARELMGATNPANANPGSIRGSFGMDISR 111

HSP 2 Score: 28.1054 bits (61), Expect = 2.111e-28
Identity = 11/24 (45.83%), Postives = 16/24 (66.67%), Query Frame = 1 
Query:  670 KRIIHGSDGPETRKDEITLWFTPE 741
            + +IHGSD   + + EI L+F PE
Sbjct:  111 RNVIHGSDSVASAEREIALYFRPE 134
[Close]
BLAST of EY695515 vs. ExPASy Swiss-Prot
Match: NDK_LISMH (Nucleoside diphosphate kinase OS=Listeria monocytogenes serotype 4a (strain HCC23) GN=ndk PE=3 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 2.112e-28
Identity = 53/111 (47.75%), Postives = 78/111 (70.27%), Query Frame = 2 
Query:  341 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGPVIAMVWEGEGVITYGRKLIGATDPQRSEPGTIRGDLAVVVGR 673
            ME+T++ +KPDGV+RGLI EI++R E+KG K+VA K++   +E A+KHY +   + FF  L  F++SGPV AMV EG+  I   R+++G T+P  ++PGTIR D A+   R
Sbjct:    1 MEQTYVMVKPDGVERGLIGEIVTRIEKKGLKIVAGKLMQIDRELAEKHYAEHIGKSFFEDLIGFITSGPVFAMVLEGDDAIATARRMMGKTNPLEADPGTIRADYAIHTNR 111

HSP 2 Score: 30.4166 bits (67), Expect = 2.112e-28
Identity = 13/30 (43.33%), Postives = 20/30 (66.67%), Query Frame = 1 
Query:  670 KRIIHGSDGPETRKDEITLWFTPETWLIHK 759
            + +IHGSD  E+ K EI L+F P+  L ++
Sbjct:  111 RNVIHGSDSLESAKREIQLFFAPQEILSYQ 140
[Close]
BLAST of EY695515 vs. ExPASy Swiss-Prot
Match: NDK_THEPX (Nucleoside diphosphate kinase OS=Thermoanaerobacter sp. (strain X514) GN=ndk PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 3.588e-28
Identity = 53/107 (49.53%), Postives = 73/107 (68.22%), Query Frame = 2 
Query:  341 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGPVIAMVWEGEGVITYGRKLIGATDPQRSEPGTIRGDLAV 661
            ME T   +KPDGV+RGLI EI+ R+E KG +L A K++ P+ E  +KHY + K +P++  L +++SSGPV AMV EGE  +   R L GAT  + + PGTIRGD A+
Sbjct:    1 METTLAIVKPDGVKRGLIGEILKRYENKGLRLKAAKVITPTIELLEKHYEEHKGKPYYKPLIQYMSSGPVFAMVLEGENAVKIVRLLNGATKVEEALPGTIRGDFAI 107

HSP 2 Score: 30.4166 bits (67), Expect = 3.588e-28
Identity = 13/19 (68.42%), Postives = 14/19 (73.68%), Query Frame = 1 
Query:  676 IIHGSDGPETRKDEITLWF 732
            IIHGSD  E+ K EI LWF
Sbjct:  113 IIHGSDSIESAKREIALWF 131
[Close]
BLAST of EY695515 vs. ExPASy Swiss-Prot
Match: NDK_THEP3 (Nucleoside diphosphate kinase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) GN=ndk PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 3.588e-28
Identity = 53/107 (49.53%), Postives = 73/107 (68.22%), Query Frame = 2 
Query:  341 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGPVIAMVWEGEGVITYGRKLIGATDPQRSEPGTIRGDLAV 661
            ME T   +KPDGV+RGLI EI+ R+E KG +L A K++ P+ E  +KHY + K +P++  L +++SSGPV AMV EGE  +   R L GAT  + + PGTIRGD A+
Sbjct:    1 METTLAIVKPDGVKRGLIGEILKRYENKGLRLKAAKVITPTIELLEKHYEEHKGKPYYKPLIQYMSSGPVFAMVLEGENAVKIVRLLNGATKVEEALPGTIRGDFAI 107

HSP 2 Score: 30.4166 bits (67), Expect = 3.588e-28
Identity = 13/19 (68.42%), Postives = 14/19 (73.68%), Query Frame = 1 
Query:  676 IIHGSDGPETRKDEITLWF 732
            IIHGSD  E+ K EI LWF
Sbjct:  113 IIHGSDSIESAKREIALWF 131
[Close]
BLAST of EY695515 vs. ExPASy Swiss-Prot
Match: NDK_METLZ (Nucleoside diphosphate kinase OS=Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) GN=ndk PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 5.168e-28
Identity = 60/123 (48.78%), Postives = 82/123 (66.67%), Query Frame = 2 
Query:  341 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGPVIAMVWEGEGVITYGRKLIGATDPQRSEPGTIRGDLAVVVGRESFMEVMAPRPA 709
            MERTF+ IKPDGVQRGL+ EI+SRFE KGFK+VA K  V ++    KHY +   +PF+ G+  +++SGPV   V EG+ V+   RK+ GAT+P  + PGTIRGD A+ +G+       +P  A
Sbjct:    1 MERTFVMIKPDGVQRGLVGEILSRFENKGFKIVAGKFGVLAESIVDKHYEEHLAKPFYPGMKAYITSGPVFRFVLEGDNVVATVRKMNGATNPTEANPGTIRGDYALSIGKNVIHAADSPESA 123
The following BLAST results are available for this feature:
BLAST of EY695515 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
NDK_THETN1.248e-2853.77Nucleoside diphosphate kinase OS=Thermoanaerobacte... [more]
NDK_CORGL1.248e-2852.73Nucleoside diphosphate kinase OS=Corynebacterium g... [more]
NDK_CORGB1.248e-2852.73Nucleoside diphosphate kinase OS=Corynebacterium g... [more]
NDK_PYRKO2.104e-2847.75Nucleoside diphosphate kinase OS=Pyrococcus kodaka... [more]
NDK_DESHY2.111e-2846.85Nucleoside diphosphate kinase OS=Desulfitobacteriu... [more]
NDK_DESHD2.111e-2846.85Nucleoside diphosphate kinase OS=Desulfitobacteriu... [more]
NDK_LISMH2.112e-2847.75Nucleoside diphosphate kinase OS=Listeria monocyto... [more]
NDK_THEPX3.588e-2849.53Nucleoside diphosphate kinase OS=Thermoanaerobacte... [more]
NDK_THEP33.588e-2849.53Nucleoside diphosphate kinase OS=Thermoanaerobacte... [more]
NDK_METLZ5.168e-2848.78Nucleoside diphosphate kinase OS=Methanocorpusculu... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-700-037-C07-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY695515 ID=EY695515; Name=EY695515; organism=Citrus sinensis; type=EST; length=903bp
CCACGCGTCCGTGGGCTTACTTACTCTCTTGTTTCAGCTTAAATTTGAGT
CTTGAGAATCCAATCCAATCCCAAGTATGAGCTCTCAGATAGTTAGATCT
GCTTCTAGAGCCGCAACCGCAAGGTCTCTGCTTTCAGCTTCCAAGAATTC
GCGCTTTTACTCCGAAGGGCGAGCTGTATCTGCTGCAGCGGCAGTTACAT
TCAGTGGGAAGTTGCCTTATCTTGTTTCAAGTTTTGGGAGGGCTGGTTCC
TCAACTGCATCTCGATCATGGCTCTCGGGAGCCATTGCAATTCCTGCTGC
AGCTTACATGCTCCAGGAGCAGGAAGTACATGCTGCAGAGATGGAACGCA
CTTTCATTGCCATCAAGCCAGATGGGGTGCAAAGAGGACTGATTTCAGAA
ATCATCTCTCGCTTTGAGCGCAAAGGCTTTAAGCTTGTGGCCATTAAGAT
AGTAGTTCCATCAAAAGAGTTTGCACAAAAACATTATCATGATCTGAAGG
AAAGACCCTTCTTTAATGGCCTGTGCGAGTTCCTTAGCTCTGGTCCAGTC
ATTGCCATGGTATGGGAAGGAGAGGGAGTTATTACGTATGGTCGCAAACT
CATTGGAGCCACAGATCCTCAGAGATCAGAACCTGGAACAATCAGAGGCG
ATCTAGCTGTTGTTGTTGGAAGAGAATCATTCATGGAAGTGATGGCCCCG
AGACCCGCAAGGATGAGATCACATTGTGGTTCACACCCGAGACTTGGTTA
ATACACAAGNCATGCAGAGAAGTGGGTCTATGAATCCACTGAGTACAATT
TTCTCTTACGTTTATAGAAACTTAGGNGCTACATGATAGTGCGATTAGAG
CATAACTGGTCCAAAAAATTTTGTTCTGTGAGATGAGTAAACACAACTTG
CTC
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