EY693007 | Citrus Genome Database

Overview


Name	EY693007
Unique Name	EY693007
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	854

Libraries


Library Name	Type
Sweet orange fruit, development stadium (1 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_PSYIN (S-adenosylmethionine synthetase OS=Psychromonas ingrahamii (strain 37) GN=metK PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.117e-14
Identity = 36/74 (48.65%), Postives = 43/74 (58.11%), Query Frame = 3

Query:  291 DKIGGYTCRSIGIVSDDGGFVADHCLGLVDIVRHRPHIAQGVDGDFTERPEDIGAGDQGLMFAYATAETPEFLP 512
            ++I   T R+IG  S + GF AD C  L  I +  P I QGVD      P + GAGDQGLMF YAT ET  F+P
Sbjct:   66 EEITRNTVRNIGYTSSEMGFDADSCAILNVIGKQSPDINQGVD---RSDPREQGAGDQGLMFGYATNETDVFMP 136

HSP 2 Score: 40.0466 bits (92), Expect = 1.117e-14
Identity = 21/34 (61.76%), Postives = 25/34 (73.53%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            QISDA LDA L QD +++VACET   T MV+V G
Sbjct:   22 QISDAVLDAILKQDKKARVACETYVKTGMVMVGG 55

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_SYNPW (S-adenosylmethionine synthetase OS=Synechococcus sp. (strain WH7803) GN=metK PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.448e-14
Identity = 37/76 (48.68%), Postives = 45/76 (59.21%), Query Frame = 3

Query:  342 GGFVADHCLGLVDIVRHRPHIAQGVD--GDFTERPED-IGAGDQGLMFAYATAETPEFLPFRRGLAIKLGAHVTEV 560
            GGF A+ C  LV + +  P IAQGVD   D    P D +GAGDQG+MF YA  ETPE +P    LA +L   + EV
Sbjct:   84 GGFDANSCAVLVALDQQSPDIAQGVDEADDHEGDPLDKVGAGDQGIMFGYACNETPELMPLPISLAHRLSRRLAEV 159

HSP 2 Score: 38.1206 bits (87), Expect = 1.448e-14
Identity = 18/34 (52.94%), Postives = 24/34 (70.59%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            Q+SDA LDA L QD+ S+VACET   T + ++ G
Sbjct:   22 QVSDAVLDALLAQDSSSRVACETVVNTGLCMITG 55

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_SYNR3 (S-adenosylmethionine synthetase OS=Synechococcus sp. (strain RCC307) GN=metK PE=3 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.449e-14
Identity = 37/76 (48.68%), Postives = 45/76 (59.21%), Query Frame = 3

Query:  342 GGFVADHCLGLVDIVRHRPHIAQGVD--GDFTERPED-IGAGDQGLMFAYATAETPEFLPFRRGLAIKLGAHVTEV 560
            GGF A+ C  LV + +  P IAQGVD   D    P D +GAGDQG+MF YA  ETPE +P    LA +L   + EV
Sbjct:   84 GGFDANSCAVLVALDQQSPDIAQGVDEADDHAGDPLDKVGAGDQGIMFGYACDETPELMPLPISLAHRLARRLAEV 159

HSP 2 Score: 37.7354 bits (86), Expect = 1.449e-14
Identity = 18/34 (52.94%), Postives = 23/34 (67.65%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            Q+SDA LDA L QD  S+VACET   T + ++ G
Sbjct:   22 QVSDAVLDALLAQDPASRVACETVVNTGLCIITG 55

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_PHOPR (S-adenosylmethionine synthetase OS=Photobacterium profundum GN=metK PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.451e-14
Identity = 33/74 (44.59%), Postives = 44/74 (59.46%), Query Frame = 3

Query:  291 DKIGGYTCRSIGIVSDDGGFVADHCLGLVDIVRHRPHIAQGVDGDFTERPEDIGAGDQGLMFAYATAETPEFLP 512
            ++I   T R IG V+ + GF A+ C  L  I +  P I QGVD    E P ++GAGDQG+MF YA+ ET   +P
Sbjct:   66 EEITRETIREIGYVNSEMGFDANSCAVLSAIGKQSPDINQGVD---REDPRELGAGDQGIMFGYASDETDVLMP 136

HSP 2 Score: 41.2022 bits (95), Expect = 1.451e-14
Identity = 21/34 (61.76%), Postives = 26/34 (76.47%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            QISDA LDA L+QD +++VACET   T MV+V G
Sbjct:   22 QISDAVLDAILEQDPKARVACETYVKTGMVMVGG 55

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_PSEHT (S-adenosylmethionine synthetase OS=Pseudoalteromonas haloplanktis (strain TAC 125) GN=metK PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.451e-14
Identity = 35/74 (47.30%), Postives = 41/74 (55.41%), Query Frame = 3

Query:  291 DKIGGYTCRSIGIVSDDGGFVADHCLGLVDIVRHRPHIAQGVDGDFTERPEDIGAGDQGLMFAYATAETPEFLP 512
            ++I   T R IG  + D GF AD C  L  I +  P I QGVD      PE+ GAGDQGLMF YA  ET   +P
Sbjct:   66 EEITRKTIRDIGYTNSDMGFDADSCAILNTIGKQSPDINQGVD---RTSPEEQGAGDQGLMFGYACNETEVLMP 136

HSP 2 Score: 41.5874 bits (96), Expect = 1.451e-14
Identity = 21/34 (61.76%), Postives = 26/34 (76.47%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            QISDA LDA L+QD+ ++VACET   T MV+V G
Sbjct:   22 QISDAVLDAILEQDSHARVACETYVKTGMVMVGG 55

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_GEOKA (S-adenosylmethionine synthetase OS=Geobacillus kaustophilus GN=metK PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.883e-14
Identity = 41/92 (44.57%), Postives = 45/92 (48.91%), Query Frame = 3

Query:  309 TCRSIGIVSDDGGFVADHCLGLVDIVRHRPHIAQGVD-------GDFT-ERPEDIGAGDQGLMFAYATAETPEFLPFRRGLAIKLGAHVTEV 560
            T R IG      GF AD C  L  I    P IA GVD       G  T E  E IGAGDQGLMF +A  ET E +P    LA +L   + EV
Sbjct:   74 TVREIGYTRAKYGFDADTCAVLTSIDEQSPDIAMGVDRALEAREGQMTDEEIEAIGAGDQGLMFGFACNETEELMPMPISLAHRLARRLAEV 165

HSP 2 Score: 39.2762 bits (90), Expect = 1.883e-14
Identity = 19/34 (55.88%), Postives = 25/34 (73.53%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            QISDA LDA L++D  ++VACET   T +V+V G
Sbjct:   24 QISDAILDAILEKDPNARVACETSVTTGLVLVSG 57

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_CLOD6 (S-adenosylmethionine synthetase OS=Clostridium difficile (strain 630) GN=metK PE=3 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.883e-14
Identity = 37/92 (40.22%), Postives = 44/92 (47.83%), Query Frame = 3

Query:  309 TCRSIGIVSDDGGFVADHCLGLVDIVRHRPHIAQGVDG--------DFTERPEDIGAGDQGLMFAYATAETPEFLPFRRGLAIKLGAHVTEV 560
            T R IG      GF  D C  +  I      IA GVD         +  E  E +GAGDQG+MF +A  ETPE +P    LA KL   +TEV
Sbjct:   72 TVREIGYTRAKYGFDCDTCAVITSIDEQSGDIAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRLTEV 163

HSP 2 Score: 40.0466 bits (92), Expect = 1.883e-14
Identity = 19/34 (55.88%), Postives = 26/34 (76.47%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            QISD+ LDA L++D +S+VACET   T +V+V G
Sbjct:   22 QISDSILDALLEKDPQSRVACETTVTTGLVLVAG 55

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_ENTFA (S-adenosylmethionine synthetase OS=Enterococcus faecalis GN=metK PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.884e-14
Identity = 38/93 (40.86%), Postives = 47/93 (50.54%), Query Frame = 3

Query:  294 KIGGYTCRSIGIVSDDGGFVADHCLGLVDIVRHRPHIAQGVDGDFTERPED----IGAGDQGLMFAYATAETPEFLPFRRGLAIKLGAHVTEV 560
            KI   T + IG      GF  +    LV I    P IAQGVD     R +D    IGAGDQGLMF +A  ETPE +P    L+ +L   + E+
Sbjct:   68 KIVRQTVKDIGYTRAKYGFDGETVAVLVAIDEQSPDIAQGVDAALEVRDQDEKDDIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRLAEL 160

HSP 2 Score: 41.2022 bits (95), Expect = 1.884e-14
Identity = 19/34 (55.88%), Postives = 26/34 (76.47%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            Q+SDA LDA L++D  ++VACET   T +V+VFG
Sbjct:   23 QVSDAILDAILEKDPMARVACETSVTTGLVLVFG 56

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_SHEFN (S-adenosylmethionine synthetase OS=Shewanella frigidimarina (strain NCIMB 400) GN=metK PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.886e-14
Identity = 39/90 (43.33%), Postives = 47/90 (52.22%), Query Frame = 3

Query:  291 DKIGGYTCRSIGIVSDDGGFVADHCLGLVDIVRHRPHIAQGVDGDFTERPEDIGAGDQGLMFAYATAETPEFLPFRRGLAIKLGAHVTEV 560
            ++I   T R IG  + D GF AD C  L  I +  P I QGVD      P + GAGDQGLMF YA+ ET   +P     A KL    +EV
Sbjct:   66 EEITRKTVREIGYTNSDMGFDADSCAVLNAIGKQSPDINQGVD---RSDPAEQGAGDQGLMFGYASNETDVLMPAPITYAHKLVKRQSEV 152

HSP 2 Score: 41.2022 bits (95), Expect = 1.886e-14
Identity = 21/34 (61.76%), Postives = 26/34 (76.47%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            QISDA LDA L+QD +++VACET   T MV+V G
Sbjct:   22 QISDAVLDAILEQDPKARVACETYVKTGMVLVGG 55

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_SYNPX (S-adenosylmethionine synthetase OS=Synechococcus sp. (strain WH8102) GN=metK PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 2.444e-14
Identity = 37/76 (48.68%), Postives = 45/76 (59.21%), Query Frame = 3

Query:  342 GGFVADHCLGLVDIVRHRPHIAQGVD--GDFTERPED-IGAGDQGLMFAYATAETPEFLPFRRGLAIKLGAHVTEV 560
            GGF A+ C  LV + +  P IAQGVD   D    P D +GAGDQG+MF YA  ETPE +P    LA +L   + EV
Sbjct:   84 GGFDANSCAVLVALDQQSPDIAQGVDEADDHEGDPLDRVGAGDQGIMFGYACNETPELMPLPISLAHRLAKRLAEV 159

HSP 2 Score: 37.3502 bits (85), Expect = 2.444e-14
Identity = 18/34 (52.94%), Postives = 23/34 (67.65%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            Q+SDA LDA L QD  S+VACET   T + ++ G
Sbjct:   22 QVSDAVLDALLAQDPSSRVACETVVNTGLCMITG 55

The following BLAST results are available for this feature:

BLAST of EY693007 vs. ExPASy Swiss-Prot

BLAST of EY693007 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 451


Match Name	E-value	Identity	Description
METK_PSYIN	1.117e-14	48.65	S-adenosylmethionine synthetase OS=Psychromonas in...	[more]
METK_SYNPW	1.448e-14	48.68	S-adenosylmethionine synthetase OS=Synechococcus s...	[more]
METK_SYNR3	1.449e-14	48.68	S-adenosylmethionine synthetase OS=Synechococcus s...	[more]
METK_PHOPR	1.451e-14	44.59	S-adenosylmethionine synthetase OS=Photobacterium ...	[more]
METK_PSEHT	1.451e-14	47.30	S-adenosylmethionine synthetase OS=Pseudoalteromon...	[more]
METK_GEOKA	1.883e-14	44.57	S-adenosylmethionine synthetase OS=Geobacillus kau...	[more]
METK_CLOD6	1.883e-14	40.22	S-adenosylmethionine synthetase OS=Clostridium dif...	[more]
METK_ENTFA	1.884e-14	40.86	S-adenosylmethionine synthetase OS=Enterococcus fa...	[more]
METK_SHEFN	1.886e-14	43.33	S-adenosylmethionine synthetase OS=Shewanella frig...	[more]
METK_SYNPX	2.444e-14	48.68	S-adenosylmethionine synthetase OS=Synechococcus s...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C3-700-006-G08-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY693007 ID=EY693007; Name=EY693007; organism=Citrus sinensis; type=EST; length=854bp
CCACGCGTCCGACCGCTTTGCTATTGAGGTCTGGTCTTAAGGCTGAATCT
GAGATCTGTGCCGAAACACTTGTGTGCCACACCTTGACAAGACGGAGACC
TCTCTATTGGCCTCTGCAATCTGTGAGCGGGGGTCACCCCGACAAGTTGA
GTCTACCAAATCTCTGATGCGGAGCTTGATGCCTGCCTTGATCAAGATGC
TGAGAGCAAGGTTGCCTGCGAGACATGCGCCATTACCTACATGGTCGTGG
TCTTTGGATAGGATTACCATCAATGCCGATGTAGACATATGATAAGATTG
GTGGCTACACTTGCCGCTCTATTGGAATTGTTTCTGATGATGGGGGTTTT
GTTGCCGATCACTGCCTAGGCCTGGTTGATATTGTGCGACACAGACCTCA
TATCGCCCAGGGTGTTGATGGTGACTTTACTGAGCGCCCTGAAGACATTG
GTGCTGGAGACCAGGGGCTTATGTTTGCTTATGCTACTGCTGAGACCCCT
GAGTTCCTGCCTTTTCGGCGCGGCCTTGCCATCAAACTTGGAGCTCACGT
CACTGAGGTCATGAATATATCACCTGCTCTTGGCTAAGGCTCCTGCGCGA
CCCAAGTCACTGGTGAGGCTACAATGCCAAGGGTGGCATATACCTGTTCG
TATTCTTACGGCCTATTTCATGCAGCATGCAGAGACTGTCCTAATGATGA
AATGCTGCTGACTCATGGATCACGTATAATGCTGCATCTCCGAAGCTTCG
TTGTTGGAAACCNTTCTCCACCTTATCCTCCCGTCNTTTTGCATTCGACG
GGCTCTTGGTGCCCTGGCTCCTTGACTTAAATCTCATCACCTTTGGGGGC
CGGG