EY693007 | Citrus Genome Database

Overview


Name	EY693007
Unique Name	EY693007
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	854

Libraries


Library Name	Type
Sweet orange fruit, development stadium (1 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_BDEBA (S-adenosylmethionine synthetase OS=Bdellovibrio bacteriovorus GN=metK PE=3 SV=1)

HSP 1 Score: 50.447 bits (119), Expect = 4.790e-11
Identity = 29/84 (34.52%), Postives = 42/84 (50.00%), Query Frame = 3

Query:  288 YDKIGGYTCRSIGIVSDDGGFVADHCLGLVDIVRHRPHIAQGVDGDFTERPEDIGAGDQGLMFAYATAETPEFLPFRRGLAIKL 539
            + ++     + IG    D GF    C  ++ + +  P IA GV    ++     GAGDQGLMF YA  ETPE +P    ++ KL
Sbjct:   65 FSEVARDVVKRIGYDHSDKGFDYKTCGVMIAVGQQSPDIAVGVKETLSDNQ---GAGDQGLMFGYAVNETPELMPLSIAMSHKL 145

HSP 2 Score: 38.5058 bits (88), Expect = 4.790e-11
Identity = 20/34 (58.82%), Postives = 23/34 (67.65%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            QISD  LDA L QD + +VACET   T +VVV G
Sbjct:   22 QISDGILDAILAQDPKGRVACETLLTTGLVVVAG 55

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_ALHEH (S-adenosylmethionine synthetase OS=Alkalilimnicola ehrlichei (strain MLHE-1) GN=metK PE=3 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 4.791e-11
Identity = 32/76 (42.11%), Postives = 45/76 (59.21%), Query Frame = 3

Query:  288 YDKIGGYTCRSIGIVSDDGGFVADHCLGLVDIV-RHRPHIAQGVDGDFTERPEDIGAGDQGLMFAYATAETPEFLP 512
            Y+ +   T + IG    + GF  + C G+++I+ +    IAQGVD D    PE+ GAGDQGLMF YA+ ET   +P
Sbjct:   65 YEAVARETVKRIGYKHHEMGFDGETC-GVINILGKQSVDIAQGVDRD---HPEEQGAGDQGLMFGYASNETDVLMP 136

HSP 2 Score: 35.039 bits (79), Expect = 4.791e-11
Identity = 16/34 (47.06%), Postives = 23/34 (67.65%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            QISDA LDA L +D   ++ACET   T ++++ G
Sbjct:   22 QISDAVLDAILAEDPRGRIACETMIKTGIIILGG 55

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_CHLCH (S-adenosylmethionine synthetase OS=Chlorobium chlorochromatii (strain CaD3) GN=metK PE=3 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 5.058e-11
Identity = 29/82 (35.37%), Postives = 42/82 (51.22%), Query Frame = 3

Query:  321 IGIVSDDGGFVADHCLGLVDIVRHRPHIAQGVDG--DFTERPEDIGAGDQGLMFAYATAETPEFLPFRRGLAIKLGAHVTEV 560
            IG    +  F A+ C  L  +    P I +GVD   +  +  + +GAGDQG+MF YA  ETPE +P     A +L   + E+
Sbjct:   78 IGYTKGEYMFEANSCGILSALHSQSPDINRGVDRKEEIADEFDRVGAGDQGMMFGYACTETPELMPAAIQFAQQLVKKLAEI 159

HSP 2 Score: 37.7354 bits (86), Expect = 5.058e-11
Identity = 20/34 (58.82%), Postives = 23/34 (67.65%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            QISDA LD  L QD+ S+VACET   T  V+V G
Sbjct:   24 QISDAVLDDFLRQDSTSRVACETFVTTGQVIVGG 57

HSP 3 Score: 22.7126 bits (47), Expect = 5.058e-11
Identity = 8/16 (50.00%), Postives = 13/16 (81.25%), Query Frame = 3

Query:  105 YWPLQSVSGGHPDKLS 152
            ++  +SVS GHPDK++
Sbjct:    7 FFTSESVSEGHPDKVA 22

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_STRST (S-adenosylmethionine synthetase OS=Streptomyces spectabilis GN=metK PE=3 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 6.205e-11
Identity = 38/87 (43.68%), Postives = 43/87 (49.43%), Query Frame = 3

Query:  321 IGIVSDDGGFVADHCLGLVDIVRHRPHIAQGVDGDFTERPEDI-------GAGDQGLMFAYATAETPEFLPFRRGLAIKLGAHVTEV 560
            IG  S   GF    C   V I    P IAQGVD  +  R E         GAGDQGLMF YA  ETPE +P    LA +L   ++EV
Sbjct:   76 IGYDSSKKGFDGASCGVSVSIGAQSPDIAQGVDTAYESRVEGDEDELDRQGAGDQGLMFGYACDETPELMPLPIHLAHRLSRRLSEV 162

HSP 2 Score: 31.5722 bits (70), Expect = 6.205e-11
Identity = 18/34 (52.94%), Postives = 21/34 (61.76%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            QISD  LDA L +D  S+VA ET   T +V V G
Sbjct:   22 QISDTILDALLREDPTSRVAVETLITTGLVHVAG 55

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_XYLFT (S-adenosylmethionine synthetase OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) GN=metK PE=3 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 6.209e-11
Identity = 31/64 (48.44%), Postives = 37/64 (57.81%), Query Frame = 3

Query:  321 IGIVSDDGGFVADHCLGLVDIVRHRPHIAQGVDGDFTERPEDIGAGDQGLMFAYATAETPEFLP 512
            IG  S D GF    C  L  I +  P I QGVD    ++ E+ GAGDQGLMF YAT ET  ++P
Sbjct:   76 IGYNSSDVGFDGATCGVLNLIGKQSPDINQGVD---RKKAEEQGAGDQGLMFGYATNETDSYMP 136

HSP 2 Score: 36.965 bits (84), Expect = 6.209e-11
Identity = 18/34 (52.94%), Postives = 23/34 (67.65%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            QISDA LDA L QD  ++VACET   T + ++ G
Sbjct:   22 QISDAVLDAILVQDPRARVACETMVKTGVAIIAG 55

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_XYLFM (S-adenosylmethionine synthetase OS=Xylella fastidiosa (strain M12) GN=metK PE=3 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 6.209e-11
Identity = 31/64 (48.44%), Postives = 37/64 (57.81%), Query Frame = 3

Query:  321 IGIVSDDGGFVADHCLGLVDIVRHRPHIAQGVDGDFTERPEDIGAGDQGLMFAYATAETPEFLP 512
            IG  S D GF    C  L  I +  P I QGVD    ++ E+ GAGDQGLMF YAT ET  ++P
Sbjct:   76 IGYNSSDVGFDGATCGVLNLIGKQSPDINQGVD---RKKAEEQGAGDQGLMFGYATNETDSYMP 136

HSP 2 Score: 36.965 bits (84), Expect = 6.209e-11
Identity = 18/34 (52.94%), Postives = 23/34 (67.65%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            QISDA LDA L QD  ++VACET   T + ++ G
Sbjct:   22 QISDAVLDAILVQDPRARVACETMVKTGVAIIAG 55

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_XYLFA (S-adenosylmethionine synthetase OS=Xylella fastidiosa GN=metK PE=3 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 6.209e-11
Identity = 31/64 (48.44%), Postives = 37/64 (57.81%), Query Frame = 3

Query:  321 IGIVSDDGGFVADHCLGLVDIVRHRPHIAQGVDGDFTERPEDIGAGDQGLMFAYATAETPEFLP 512
            IG  S D GF    C  L  I +  P I QGVD    ++ E+ GAGDQGLMF YAT ET  ++P
Sbjct:   76 IGYNSSDVGFDGATCGVLNLIGKQSPDINQGVD---RKKAEEQGAGDQGLMFGYATNETDSYMP 136

HSP 2 Score: 36.965 bits (84), Expect = 6.209e-11
Identity = 18/34 (52.94%), Postives = 23/34 (67.65%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            QISDA LDA L QD  ++VACET   T + ++ G
Sbjct:   22 QISDAVLDAILVQDPRARVACETMVKTGVAIIAG 55

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_XYLF2 (S-adenosylmethionine synthetase OS=Xylella fastidiosa (strain M23) GN=metK PE=3 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 6.209e-11
Identity = 31/64 (48.44%), Postives = 37/64 (57.81%), Query Frame = 3

Query:  321 IGIVSDDGGFVADHCLGLVDIVRHRPHIAQGVDGDFTERPEDIGAGDQGLMFAYATAETPEFLP 512
            IG  S D GF    C  L  I +  P I QGVD    ++ E+ GAGDQGLMF YAT ET  ++P
Sbjct:   76 IGYNSSDVGFDGATCGVLNLIGKQSPDINQGVD---RKKAEEQGAGDQGLMFGYATNETDSYMP 136

HSP 2 Score: 36.965 bits (84), Expect = 6.209e-11
Identity = 18/34 (52.94%), Postives = 23/34 (67.65%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            QISDA LDA L QD  ++VACET   T + ++ G
Sbjct:   22 QISDAVLDAILVQDPRARVACETMVKTGVAIIAG 55

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_RALSO (S-adenosylmethionine synthetase OS=Ralstonia solanacearum GN=metK PE=3 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 6.212e-11
Identity = 34/79 (43.04%), Postives = 41/79 (51.90%), Query Frame = 3

Query:  288 YDKIGGYTCRSIGIVSDDGGFVADHCLGLVDIVRHRPHIAQGVD---GDFTERPEDIGAGDQGLMFAYATAETPEFLPF 515
            Y  +   T + IG  + D G     C  LV   +  P IAQGVD    D+  +    GAGDQGLMF YA  ETPE +PF
Sbjct:   66 YIHVARETIKRIGYDNTDYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQ----GAGDQGLMFGYACDETPELMPF 140

HSP 2 Score: 34.2686 bits (77), Expect = 6.212e-11
Identity = 19/34 (55.88%), Postives = 23/34 (67.65%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            QISDA LDA L QD  ++VA ET   T +VV+ G
Sbjct:   23 QISDAILDAILAQDKYARVAAETLCNTGLVVLAG 56

[Close]

BLAST of EY693007 vs. ExPASy Swiss-Prot
Match: METK_FERNB (S-adenosylmethionine synthetase OS=Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1) GN=metK PE=3 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 6.212e-11
Identity = 38/92 (41.30%), Postives = 44/92 (47.83%), Query Frame = 3

Query:  309 TCRSIGIVSDDGGFVADHCLGLVDIVRHRPHIAQGVD-------GDFTERP-EDIGAGDQGLMFAYATAETPEFLPFRRGLAIKLGAHVTEV 560
            T   IG      GF  + C  L  I    P IA GV+       GD  E   E +GAGDQG+MF YAT ET E +P    LA KL   + EV
Sbjct:   71 TVLDIGYTRAKYGFDGETCAVLSSIHSQSPDIALGVNEAAEFKQGDKAEDEIEKVGAGDQGMMFGYATNETEELMPLPIMLAHKLAMKLAEV 162

HSP 2 Score: 35.039 bits (79), Expect = 6.212e-11
Identity = 17/34 (50.00%), Postives = 24/34 (70.59%), Query Frame = 1

Query:  157 QISDAELDACLDQDAESKVACETCAITYMVVVFG 258
            QISDA LDA ++QD +++VA ET   T + +V G
Sbjct:   21 QISDAILDAIIEQDPKARVAVETLLTTGVAIVAG 54

The following BLAST results are available for this feature:

BLAST of EY693007 vs. ExPASy Swiss-Prot

BLAST of EY693007 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 451


Match Name	E-value	Identity	Description
METK_BDEBA	4.790e-11	34.52	S-adenosylmethionine synthetase OS=Bdellovibrio ba...	[more]
METK_ALHEH	4.791e-11	42.11	S-adenosylmethionine synthetase OS=Alkalilimnicola...	[more]
METK_CHLCH	5.058e-11	35.37	S-adenosylmethionine synthetase OS=Chlorobium chlo...	[more]
METK_STRST	6.205e-11	43.68	S-adenosylmethionine synthetase OS=Streptomyces sp...	[more]
METK_XYLFT	6.209e-11	48.44	S-adenosylmethionine synthetase OS=Xylella fastidi...	[more]
METK_XYLFM	6.209e-11	48.44	S-adenosylmethionine synthetase OS=Xylella fastidi...	[more]
METK_XYLFA	6.209e-11	48.44	S-adenosylmethionine synthetase OS=Xylella fastidi...	[more]
METK_XYLF2	6.209e-11	48.44	S-adenosylmethionine synthetase OS=Xylella fastidi...	[more]
METK_RALSO	6.212e-11	43.04	S-adenosylmethionine synthetase OS=Ralstonia solan...	[more]
METK_FERNB	6.212e-11	41.30	S-adenosylmethionine synthetase OS=Fervidobacteriu...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C3-700-006-G08-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY693007 ID=EY693007; Name=EY693007; organism=Citrus sinensis; type=EST; length=854bp
CCACGCGTCCGACCGCTTTGCTATTGAGGTCTGGTCTTAAGGCTGAATCT
GAGATCTGTGCCGAAACACTTGTGTGCCACACCTTGACAAGACGGAGACC
TCTCTATTGGCCTCTGCAATCTGTGAGCGGGGGTCACCCCGACAAGTTGA
GTCTACCAAATCTCTGATGCGGAGCTTGATGCCTGCCTTGATCAAGATGC
TGAGAGCAAGGTTGCCTGCGAGACATGCGCCATTACCTACATGGTCGTGG
TCTTTGGATAGGATTACCATCAATGCCGATGTAGACATATGATAAGATTG
GTGGCTACACTTGCCGCTCTATTGGAATTGTTTCTGATGATGGGGGTTTT
GTTGCCGATCACTGCCTAGGCCTGGTTGATATTGTGCGACACAGACCTCA
TATCGCCCAGGGTGTTGATGGTGACTTTACTGAGCGCCCTGAAGACATTG
GTGCTGGAGACCAGGGGCTTATGTTTGCTTATGCTACTGCTGAGACCCCT
GAGTTCCTGCCTTTTCGGCGCGGCCTTGCCATCAAACTTGGAGCTCACGT
CACTGAGGTCATGAATATATCACCTGCTCTTGGCTAAGGCTCCTGCGCGA
CCCAAGTCACTGGTGAGGCTACAATGCCAAGGGTGGCATATACCTGTTCG
TATTCTTACGGCCTATTTCATGCAGCATGCAGAGACTGTCCTAATGATGA
AATGCTGCTGACTCATGGATCACGTATAATGCTGCATCTCCGAAGCTTCG
TTGTTGGAAACCNTTCTCCACCTTATCCTCCCGTCNTTTTGCATTCGACG
GGCTCTTGGTGCCCTGGCTCCTTGACTTAAATCTCATCACCTTTGGGGGC
CGGG