EY691939

Overview
NameEY691939
Unique NameEY691939
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length912
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_METKA (Inorganic pyrophosphatase OS=Methanopyrus kandleri GN=ppa PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 4.928e-19
Identity = 29/66 (43.94%), Postives = 41/66 (62.12%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEEKNG*DHLVAGAEESP 657
            P++YGFIPRTL +D DPLD+L+IMQ+P  PGC +   RP      +D  ++    D ++A   E P
Sbjct:   54 PLDYGFIPRTLYDDGDPLDILVIMQDPTFPGCVIEA-RPIGLMKMLDDSDQD---DKVLAVPTEDP 115

HSP 2 Score: 56.225 bits (134), Expect = 4.928e-19
Identity = 26/50 (52.00%), Postives = 32/50 (64.00%), Query Frame = 3 
Query:  312 WHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYP 461
            W DLE GP  P +   VIEI +GS+ KYE D++ G   +DRVLYS   YP
Sbjct:    5 WKDLEPGPNPPDVVYAVIEIPRGSRNKYEYDEERGFFKLDRVLYSPFHYP 54
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_PYRAB (Inorganic pyrophosphatase OS=Pyrococcus abyssi GN=ppa PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 6.409e-19
Identity = 31/52 (59.62%), Postives = 40/52 (76.92%), Query Frame = 3 
Query:  306 HPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYP 461
            +P+HDLE GP  P++   +IEI KGS+ KYELDKK+GL+ +DRVLYS   YP
Sbjct:    2 NPFHDLEPGPNVPEVVYALIEIPKGSRNKYELDKKTGLLKLDRVLYSPFFYP 53

HSP 2 Score: 46.595 bits (109), Expect = 6.409e-19
Identity = 21/51 (41.18%), Postives = 32/51 (62.75%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEE 612
            P++YG IPRT  +D+DP D+++IM+EP  P   +   RP      ID G++
Sbjct:   53 PVDYGIIPRTWYDDDDPFDIMVIMREPTYPLTIIEA-RPIGLFKMIDSGDK 102
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_PYRHO (Inorganic pyrophosphatase OS=Pyrococcus horikoshii GN=ppa PE=1 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 4.032e-18
Identity = 29/52 (55.77%), Postives = 40/52 (76.92%), Query Frame = 3 
Query:  306 HPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYP 461
            +P+HDLE GP  P++   +IEI KGS+ KYELDK++GL+ +DRVLY+   YP
Sbjct:    2 NPFHDLEPGPNVPEVVYALIEIPKGSRNKYELDKETGLLKLDRVLYTPFHYP 53

HSP 2 Score: 47.3654 bits (111), Expect = 4.032e-18
Identity = 22/51 (43.14%), Postives = 31/51 (60.78%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEE 612
            P++YG IPRT  ED DP D+++IM+EP  P   +   RP      ID G++
Sbjct:   53 PVDYGIIPRTWYEDGDPFDIMVIMREPTYPLTIIEA-RPIGLFKMIDSGDK 102
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR2_CHLRE (Soluble inorganic pyrophosphatase 2 OS=Chlamydomonas reinhardtii GN=ppa2 PE=1 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 2.445e-17
Identity = 41/62 (66.13%), Postives = 46/62 (74.19%), Query Frame = 3 
Query:  291 RSVAAHPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            R  A+HPWHDL  G  AP   +CVIEI +GSKVKYELDK +GL  VDR+LYSSVVYPH   F
Sbjct:    5 RGTASHPWHDLHPGNDAPNFVSCVIEIPRGSKVKYELDKDTGLCFVDRILYSSVVYPHNYGF 66

HSP 2 Score: 73.559 bits (179), Expect = 1.823e-12
Identity = 45/123 (36.59%), Postives = 67/123 (54.47%), Query Frame = 1 
Query:  364 LKLGKGAR*NMNLTKNLD*SWLIVFFTLQLCTPINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEEKNG*DHLVAGAEESPSTEHYHGP*GAFPHLFGVKIPRFFE 732
            +++ +G++    L K+    ++       +  P NYGF+P+TLCED DPLDVL++MQEPV+P CFL   +P      +DQGE     D L+A   + P  + +       PH    +I RFFE
Sbjct:   29 IEIPRGSKVKYELDKDTGLCFVDRILYSSVVYPHNYGFVPKTLCEDGDPLDVLVLMQEPVVPMCFLRA-KPIGVMQMLDQGERD---DKLIAVHADDPEYKGFTDISQLPPHRL-AEIKRFFE 146
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_THEAC (Inorganic pyrophosphatase OS=Thermoplasma acidophilum GN=ppa PE=1 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 2.067e-16
Identity = 25/50 (50.00%), Postives = 36/50 (72.00%), Query Frame = 3 
Query:  312 WHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYP 461
            +H + +GP  P+    ++EI +GS+VKYE+ K    ++VDRVLYSSVVYP
Sbjct:    5 YHSVPVGPKPPEEVYVIVEIPRGSRVKYEIAKDFPGMLVDRVLYSSVVYP 54

HSP 2 Score: 51.9878 bits (123), Expect = 2.067e-16
Identity = 23/53 (43.40%), Postives = 33/53 (62.26%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEEKN 618
            P++YG IPRTL  D DP+DV++++ +P  PG  +   RP      +DQGE  N
Sbjct:   54 PVDYGLIPRTLYYDGDPMDVMVLISQPTFPGAIMKV-RPIGMMKMVDQGETDN 105
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_HALSA (Inorganic pyrophosphatase OS=Halobacterium salinarium GN=ppa PE=3 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 5.906e-16
Identity = 27/58 (46.55%), Postives = 36/58 (62.07%), Query Frame = 3 
Query:  303 AHPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            A+ W DLE GP AP +   V+E  KG + KYE DK    +++DRVL+S+V YP    F
Sbjct:    2 ANLWEDLETGPDAPDVIYAVVECLKGERNKYEYDKDIPGVVLDRVLHSNVHYPSDYGF 59

HSP 2 Score: 50.0618 bits (118), Expect = 5.906e-16
Identity = 25/66 (37.88%), Postives = 38/66 (57.58%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEEKNG*DHLVAGAEESP 657
            P +YGFIP++  +D DP DVL+++++   PGC +   RP       D GE+    D ++A  EE P
Sbjct:   54 PSDYGFIPQSYYDDGDPFDVLVLVEDQTFPGCVIEA-RPVALMEMDDDGEQD---DKVIAVPEEDP 115
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_ANASP (Inorganic pyrophosphatase OS=Anabaena sp. (strain PCC 7120) GN=ppa PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 1.001e-15
Identity = 32/90 (35.56%), Postives = 47/90 (52.22%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEEKNG*DHLVAGAEESPSTEHYHGP*GAFPHLFGVKIPRFF 729
            P +YGF+P TL +D DPLD ++I+ EP  PGC ++  RP  F   ID G+     + ++A  ++ P   H        PH    +I  FF
Sbjct:   51 PYDYGFVPNTLADDGDPLDGMVIIDEPTFPGCVIAA-RPIGFLEMIDGGDRD---EKILAVPDKDPRYAHVKSLNDVAPHRLD-EIAEFF 135

HSP 2 Score: 47.7506 bits (112), Expect = 1.001e-15
Identity = 27/55 (49.09%), Postives = 32/55 (58.18%), Query Frame = 3 
Query:  318 DLEIGPGAPK--IFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            DL   P  PK  + N +IEI  GS+ KYE DK      +DRVLYSSV YP+   F
Sbjct:    2 DLSRIPAQPKPGVINILIEIAGGSQNKYEFDKDLEAFALDRVLYSSVKYPYDYGF 56
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_THEEB (Inorganic pyrophosphatase OS=Thermosynechococcus elongatus (strain BP-1) GN=ppa PE=3 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 1.689e-15
Identity = 25/50 (50.00%), Postives = 31/50 (62.00%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGE 609
            P +YGFIP TL +D DPLD L++M EP  PGC +   RP      ID G+
Sbjct:   53 PYDYGFIPNTLADDGDPLDGLVMMDEPTFPGCVIPA-RPIGMLEMIDSGD 101

HSP 2 Score: 50.447 bits (119), Expect = 1.689e-15
Identity = 26/55 (47.27%), Postives = 35/55 (63.64%), Query Frame = 3 
Query:  318 DLEIGPGAPK--IFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            DL   P  PK  + N ++EI  GS+ KYE DK+  +  +DRVLYS+VVYP+   F
Sbjct:    4 DLSRIPAQPKPGVVNVLVEIAGGSRNKYEFDKEMKVFALDRVLYSAVVYPYDYGF 58
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_SYNY3 (Inorganic pyrophosphatase OS=Synechocystis sp. (strain PCC 6803) GN=ppa PE=1 SV=2)

HSP 1 Score: 52.7582 bits (125), Expect = 4.825e-15
Identity = 24/50 (48.00%), Postives = 32/50 (64.00%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGE 609
            P +YGFIP TL +D DPLD ++IM +P  PGC ++  RP      ID G+
Sbjct:   51 PYDYGFIPNTLADDGDPLDGMVIMDQPTFPGCVITA-RPIGMLEMIDGGD 99

HSP 2 Score: 49.6766 bits (117), Expect = 4.825e-15
Identity = 28/55 (50.91%), Postives = 32/55 (58.18%), Query Frame = 3 
Query:  318 DLEIGPGAPK--IFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            DL   P  PK  + N +IEI  GSK KYE DK      +DRVLYSSV YP+   F
Sbjct:    2 DLSRIPAQPKAGLINVLIEIPAGSKNKYEFDKDMNCFALDRVLYSSVQYPYDYGF 56
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_SULTO (Inorganic pyrophosphatase OS=Sulfolobus tokodaii GN=ppa PE=1 SV=2)

HSP 1 Score: 50.447 bits (119), Expect = 2.322e-14
Identity = 24/54 (44.44%), Postives = 36/54 (66.67%), Query Frame = 3 
Query:  321 LEIGPG--APKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            +++ PG  AP   N +IEI  GS +KYE D++  ++ VDR+LY+S+VYP    F
Sbjct:    1 MKLSPGKKAPDEVNVLIEIPLGSNIKYEYDEEEEVVKVDRILYTSMVYPFNYGF 54

HSP 2 Score: 49.6766 bits (117), Expect = 2.322e-14
Identity = 22/31 (70.97%), Postives = 24/31 (77.42%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPG 552
            P NYGFIP TL ED DPLDVL+I   P+LPG
Sbjct:   49 PFNYGFIPGTLEEDGDPLDVLVISNYPLLPG 79
The following BLAST results are available for this feature:
BLAST of EY691939 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 42
Match NameE-valueIdentityDescription
IPYR_METKA4.928e-1943.94Inorganic pyrophosphatase OS=Methanopyrus kandleri... [more]
IPYR_PYRAB6.409e-1959.62Inorganic pyrophosphatase OS=Pyrococcus abyssi GN=... [more]
IPYR_PYRHO4.032e-1855.77Inorganic pyrophosphatase OS=Pyrococcus horikoshii... [more]
IPYR2_CHLRE2.445e-1766.13Soluble inorganic pyrophosphatase 2 OS=Chlamydomon... [more]
IPYR_THEAC2.067e-1650.00Inorganic pyrophosphatase OS=Thermoplasma acidophi... [more]
IPYR_HALSA5.906e-1646.55Inorganic pyrophosphatase OS=Halobacterium salinar... [more]
IPYR_ANASP1.001e-1535.56Inorganic pyrophosphatase OS=Anabaena sp. (strain ... [more]
IPYR_THEEB1.689e-1550.00Inorganic pyrophosphatase OS=Thermosynechococcus e... [more]
IPYR_SYNY34.825e-1548.00Inorganic pyrophosphatase OS=Synechocystis sp. (st... [more]
IPYR_SULTO2.322e-1444.44Inorganic pyrophosphatase OS=Sulfolobus tokodaii G... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C2-003-102-E02-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY691939 ID=EY691939; Name=EY691939; organism=Citrus sinensis; type=EST; length=912bp
GAAAGAGAGCGGGAAGGGAACCAGCGTCAGTTCTCTTGCTTTCCTCTACT
TAAGCGCCCGTAGAGGAACTTAACTTTAATATCTTCGCCATTACGCCTCT
CTCTTTTCGAGATCGGTGCGTTTCTTTGTGGTCTTGAAGCTTCGTTCTCG
TTAGGCCCTGTTTGGCTTTCAGTACTTGAACCGTTACGGTTATGATGGCT
CCACCAATTGAGACTTCAAGCAAAACTCCTGTCTCCCAACCTCCAGCACA
TCCTCCGCTTAATGAAAGGATACTTTCTTCAATGAGCAGGAGGTCTGTTG
CTGCACATCCTTGGCATGATCTTGAGATTGGACCTGGAGCTCCAAAGATT
TTCAATTGCGTGATTGAAATTGGGAAAGGGAGCAAGGTGAAATATGAACT
TGACAAAAAATCTGGACTGATCATGGTTGATCGTGTTCTTTACTCTTCAG
TTGTGTACCCCCATAAACTATGGTTTCATACCTCGTACTCTTTGCGAAGA
CAATGATCCCCTTGATGTCCTGATCATCATGCAGGAGCCAGTTCTTCCAG
GCTGCTTTCTTTCGGGCTAAAGGCCATCGGAATTCTGCCTATGAATTGAT
CAGGGGGAAGAAAAGAATGGATAAGATCATTTGGTGGCCGGGGCTGAAGA
ATCCCCAAGTACCGAACACTACCATGGACCTTAAGGGGCTTTCCCCCATT
TGTTTGGGGTGAAAATCCCCCGCTTTTTTGAAAAAAAACAAAAAAAATGG
AAAAAAAGGAAAGTTGCGGTTAATGGATTTTTTGGCCAACCCCTGGAGGC
GTAAAAATTTTATTTCCAGGAGTTTTATGAAACCCCTTAGGGTGGAATAT
ATTTGGGGGGAAGAGTTTGGGGGGGGGGGAACTCGTTTTGGTTCCCCCCT
GGGGGTGAAAAA
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