EY691939

Overview
NameEY691939
Unique NameEY691939
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length912
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_SULSO (Inorganic pyrophosphatase OS=Sulfolobus solfataricus GN=ppa PE=3 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 3.920e-14
Identity = 27/54 (50.00%), Postives = 36/54 (66.67%), Query Frame = 3 
Query:  321 LEIGPG--APKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            +++GPG  AP   N  IEI  GS +KYE D++  +I VDRVLY+S+VYP    F
Sbjct:    1 MKLGPGKKAPDEINVFIEIPMGSNIKYEYDEEEDIIKVDRVLYTSMVYPFNYGF 54

HSP 2 Score: 45.0542 bits (105), Expect = 3.920e-14
Identity = 19/31 (61.29%), Postives = 23/31 (74.19%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPG 552
            P NYGFIP TL ED DPLDVL++    ++PG
Sbjct:   49 PFNYGFIPETLEEDGDPLDVLVLGNYSLMPG 79
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_SULAC (Inorganic pyrophosphatase OS=Sulfolobus acidocaldarius GN=ppa PE=1 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 5.088e-14
Identity = 25/52 (48.08%), Postives = 34/52 (65.38%), Query Frame = 3 
Query:  321 LEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            L  G  AP + N ++EI +GS +KYE D + G+I VDRVLY+S+ YP    F
Sbjct:    3 LSPGKNAPDVVNVLVEIPQGSNIKYEYDDEEGVIKVDRVLYTSMNYPFNYGF 54

HSP 2 Score: 44.669 bits (104), Expect = 5.088e-14
Identity = 20/31 (64.52%), Postives = 22/31 (70.97%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPG 552
            P NYGFIP TL ED DPLDVL+I    + PG
Sbjct:   49 PFNYGFIPGTLEEDGDPLDVLVITNYQLYPG 79
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_CAUCR (Inorganic pyrophosphatase OS=Caulobacter crescentus GN=ppa PE=3 SV=1)

HSP 1 Score: 48.1358 bits (113), Expect = 6.963e-13
Identity = 23/53 (43.40%), Postives = 35/53 (66.04%), Query Frame = 3 
Query:  321 LEIGPGAPKIFNCVIEIGKGSK-VKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            + +G   P   N +IEI +G + VKYE+DK+SG +MVDR L++++ YP    F
Sbjct:    6 IAVGVNPPYDLNAIIEIPQGGEPVKYEIDKESGALMVDRFLHTAMFYPANYGF 58

HSP 2 Score: 46.9802 bits (110), Expect = 6.963e-13
Identity = 17/34 (50.00%), Postives = 24/34 (70.59%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFL 561
            P NYGFIP TL +D DP D++++   PV+PG  +
Sbjct:   53 PANYGFIPHTLADDGDPADIMVVGPTPVVPGAII 86
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_STRCO (Inorganic pyrophosphatase OS=Streptomyces coelicolor GN=ppa PE=3 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 1.182e-12
Identity = 27/70 (38.57%), Postives = 35/70 (50.00%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEEKNG*DHLVAGAEESPSTEH 669
            P +YGF+  TL ED DPLD L+I+ EP  PGC +       F +     +E  G D L+      P  EH
Sbjct:   39 PTDYGFVENTLGEDGDPLDALVILDEPTFPGCLIRCRAIGMFRM----TDEAGGDDKLLCVPSTDPRVEH 104

HSP 2 Score: 42.3578 bits (98), Expect = 1.182e-12
Identity = 19/37 (51.35%), Postives = 27/37 (72.97%), Query Frame = 3 
Query:  351 FNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYP 461
            F+  IEI KGS+ KYE+D ++G I +DR L++S  YP
Sbjct:    3 FDVTIEIPKGSRNKYEVDHETGRIRLDRRLFTSTAYP 39
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_CAMJE (Inorganic pyrophosphatase OS=Campylobacter jejuni GN=ppa PE=3 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 1.986e-12
Identity = 25/45 (55.56%), Postives = 30/45 (66.67%), Query Frame = 3 
Query:  342 PKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            P   N VIEI  GS +KYE+DK SG IMVDRV+ S++ YP    F
Sbjct:   12 PNKINAVIEIPYGSNIKYEIDKDSGAIMVDRVMASAIFYPANYGF 56

HSP 2 Score: 41.2022 bits (95), Expect = 1.986e-12
Identity = 15/34 (44.12%), Postives = 23/34 (67.65%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFL 561
            P NYGFI  TL +D DP+D+L++ + P+  G  +
Sbjct:   51 PANYGFIANTLADDGDPVDILVLNEYPIQAGAVI 84
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_BACHD (Inorganic pyrophosphatase OS=Bacillus halodurans GN=ppa PE=3 SV=1)

HSP 1 Score: 48.521 bits (114), Expect = 1.994e-12
Identity = 28/66 (42.42%), Postives = 34/66 (51.52%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEEKNG*DHLVAGAEESP 657
            P  YG+I  TL  D DPLDVL+I+  P  PGC +   +   F   ID GEE      L+A   E P
Sbjct:   44 PAEYGYIENTLALDGDPLDVLVIVSNPTFPGCVIDA-KVLGFLNMIDGGEED---QKLIAVPTEDP 105

HSP 2 Score: 45.0542 bits (105), Expect = 1.994e-12
Identity = 20/40 (50.00%), Postives = 27/40 (67.50%), Query Frame = 3 
Query:  342 PKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYP 461
            PK+    IEI  GS+ KYE DK+ G+  +DRVL+S + YP
Sbjct:    5 PKVIEAFIEIPTGSQNKYEYDKERGVFKLDRVLFSPMFYP 44
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_BRAJA (Inorganic pyrophosphatase OS=Bradyrhizobium japonicum GN=ppa PE=3 SV=1)

HSP 1 Score: 46.595 bits (109), Expect = 4.342e-12
Identity = 28/71 (39.44%), Postives = 36/71 (50.70%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEEKNG*DHLVAGAEESPSTEHY 672
            P NYGFIP TL +D DP DVLII    ++PG  +S  RP          E++ G D  +     S  T+ Y
Sbjct:   53 PGNYGFIPHTLSDDGDPCDVLIINTRAIIPGAVMSV-RPVGVLF----MEDEAGGDEKILAVPSSKLTQRY 118

HSP 2 Score: 45.8246 bits (107), Expect = 4.342e-12
Identity = 21/53 (39.62%), Postives = 34/53 (64.15%), Query Frame = 3 
Query:  321 LEIGPGAPKIFNCVIEIGKGSK-VKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            + IG   P+  N +IE+  G + +KYE+DK++G ++VDR LY+ + YP    F
Sbjct:    6 ISIGKNVPQDVNVIIEVPVGGEPIKYEMDKEAGTLVVDRFLYTPMRYPGNYGF 58
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_NANEQ (Inorganic pyrophosphatase OS=Nanoarchaeum equitans GN=ppa PE=3 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 4.374e-12
Identity = 22/34 (64.71%), Postives = 28/34 (82.35%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFL 561
            P NYGFIP TL ED DPLDVL+I +EP++PG ++
Sbjct:   37 PYNYGFIPETLEEDGDPLDVLVITEEPLVPGSYI 70

HSP 2 Score: 38.5058 bits (88), Expect = 4.374e-12
Identity = 18/38 (47.37%), Postives = 25/38 (65.79%), Query Frame = 3 
Query:  363 IEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            IE+ KGS VKYE +   G + VDR+LY ++ YP+   F
Sbjct:    7 IEVPKGSNVKYEYE--DGKLKVDRILYGAMFYPYNYGF 42
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_XANCP (Inorganic pyrophosphatase OS=Xanthomonas campestris pv. campestris GN=ppa PE=3 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 5.638e-12
Identity = 17/31 (54.84%), Postives = 24/31 (77.42%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPG 552
            P NYG++P TLC D DP DVL+++  P++PG
Sbjct:   53 PCNYGYVPNTLCGDGDPADVLVVLPLPLVPG 83

HSP 2 Score: 44.2838 bits (103), Expect = 5.638e-12
Identity = 24/45 (53.33%), Postives = 30/45 (66.67%), Query Frame = 3 
Query:  330 GPGAPKIFNCVIEIGKGSK-VKYELDKKSGLIMVDRVLYSSVVYP 461
            G   P+  N VIEI K S+ VKYE+DK SG I VDR+L + + YP
Sbjct:    9 GKNLPEEINVVIEIPKDSEPVKYEVDKASGAIFVDRILSTPMRYP 53
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_XANAC (Inorganic pyrophosphatase OS=Xanthomonas axonopodis pv. citri GN=ppa PE=3 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 5.638e-12
Identity = 17/31 (54.84%), Postives = 24/31 (77.42%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPG 552
            P NYG++P TLC D DP DVL+++  P++PG
Sbjct:   53 PCNYGYVPNTLCGDGDPADVLVVLPLPLVPG 83

HSP 2 Score: 44.2838 bits (103), Expect = 5.638e-12
Identity = 24/45 (53.33%), Postives = 30/45 (66.67%), Query Frame = 3 
Query:  330 GPGAPKIFNCVIEIGKGSK-VKYELDKKSGLIMVDRVLYSSVVYP 461
            G   P+  N VIEI K S+ VKYE+DK SG I VDR+L + + YP
Sbjct:    9 GKNLPEEINVVIEIPKDSEPVKYEVDKASGAIFVDRILSTPMRYP 53
The following BLAST results are available for this feature:
BLAST of EY691939 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 42
Match NameE-valueIdentityDescription
IPYR_SULSO3.920e-1450.00Inorganic pyrophosphatase OS=Sulfolobus solfataric... [more]
IPYR_SULAC5.088e-1448.08Inorganic pyrophosphatase OS=Sulfolobus acidocalda... [more]
IPYR_CAUCR6.963e-1343.40Inorganic pyrophosphatase OS=Caulobacter crescentu... [more]
IPYR_STRCO1.182e-1238.57Inorganic pyrophosphatase OS=Streptomyces coelicol... [more]
IPYR_CAMJE1.986e-1255.56Inorganic pyrophosphatase OS=Campylobacter jejuni ... [more]
IPYR_BACHD1.994e-1242.42Inorganic pyrophosphatase OS=Bacillus halodurans G... [more]
IPYR_BRAJA4.342e-1239.44Inorganic pyrophosphatase OS=Bradyrhizobium japoni... [more]
IPYR_NANEQ4.374e-1264.71Inorganic pyrophosphatase OS=Nanoarchaeum equitans... [more]
IPYR_XANCP5.638e-1254.84Inorganic pyrophosphatase OS=Xanthomonas campestri... [more]
IPYR_XANAC5.638e-1254.84Inorganic pyrophosphatase OS=Xanthomonas axonopodi... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C2-003-102-E02-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY691939 ID=EY691939; Name=EY691939; organism=Citrus sinensis; type=EST; length=912bp
GAAAGAGAGCGGGAAGGGAACCAGCGTCAGTTCTCTTGCTTTCCTCTACT
TAAGCGCCCGTAGAGGAACTTAACTTTAATATCTTCGCCATTACGCCTCT
CTCTTTTCGAGATCGGTGCGTTTCTTTGTGGTCTTGAAGCTTCGTTCTCG
TTAGGCCCTGTTTGGCTTTCAGTACTTGAACCGTTACGGTTATGATGGCT
CCACCAATTGAGACTTCAAGCAAAACTCCTGTCTCCCAACCTCCAGCACA
TCCTCCGCTTAATGAAAGGATACTTTCTTCAATGAGCAGGAGGTCTGTTG
CTGCACATCCTTGGCATGATCTTGAGATTGGACCTGGAGCTCCAAAGATT
TTCAATTGCGTGATTGAAATTGGGAAAGGGAGCAAGGTGAAATATGAACT
TGACAAAAAATCTGGACTGATCATGGTTGATCGTGTTCTTTACTCTTCAG
TTGTGTACCCCCATAAACTATGGTTTCATACCTCGTACTCTTTGCGAAGA
CAATGATCCCCTTGATGTCCTGATCATCATGCAGGAGCCAGTTCTTCCAG
GCTGCTTTCTTTCGGGCTAAAGGCCATCGGAATTCTGCCTATGAATTGAT
CAGGGGGAAGAAAAGAATGGATAAGATCATTTGGTGGCCGGGGCTGAAGA
ATCCCCAAGTACCGAACACTACCATGGACCTTAAGGGGCTTTCCCCCATT
TGTTTGGGGTGAAAATCCCCCGCTTTTTTGAAAAAAAACAAAAAAAATGG
AAAAAAAGGAAAGTTGCGGTTAATGGATTTTTTGGCCAACCCCTGGAGGC
GTAAAAATTTTATTTCCAGGAGTTTTATGAAACCCCTTAGGGTGGAATAT
ATTTGGGGGGAAGAGTTTGGGGGGGGGGGAACTCGTTTTGGTTCCCCCCT
GGGGGTGAAAAA
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