EY711022 | Citrus Genome Database

Overview


Name	EY711022
Unique Name	EY711022
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	886

Libraries


Library Name	Type
Sweet orange fruit, development stadium (3 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY711022 vs. ExPASy Swiss-Prot
Match: DNAK_FRAP2 (Chaperone protein dnaK OS=Francisella philomiragia subsp. philomiragia (strain ATCC 25017) GN=dnaK PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 7.773e-12
Identity = 31/73 (42.47%), Postives = 42/73 (57.53%), Query Frame = 1

Query:  508 PPKKEQAFSTYSDYRPEVLIHSYLAETTLTMDHELLR*FDISDITTCPNEDPQITVSLHIDDHVILHVSAEDR 726
            P KK Q FST  D +P V IH    E  +   ++ L  FD++DI   P   PQI V+  ID + IL+VSA+D+
Sbjct:  421 PTKKSQVFSTAEDNQPAVTIHVLQGEREMASANKSLGRFDLADIPPAPRGIPQIEVTFDIDANGILNVSAKDK 493

HSP 2 Score: 27.7202 bits (60), Expect = 7.773e-12
Identity = 29/95 (30.53%), Postives = 40/95 (42.11%), Query Frame = 3

Query:  228 TKALCTHALLVGVSTRIPKGLHLLRHFFYAKDLCPILTPXXXXXXXXXXXXXILI*NGNYNDHDQLLLYGTPLPHDLNTARGHMTVLIPKKQPSP 512
            +K+  T  LLVG  TR+P     ++ FF  K+    + P             +L   G+  D   LLL  TPL   + T  G MT LI +    P
Sbjct:  332 SKSDITEVLLVGGQTRMPLVQEKVKEFF-GKEPRKDVNPDEAVAVGAAIQGGVLA--GDVKD--VLLLDVTPLSLGIETMGGVMTKLIERNTTIP 421

HSP 3 Score: 26.5646 bits (57), Expect = 7.773e-12
Identity = 13/35 (37.14%), Postives = 20/35 (57.14%), Query Frame = 2

Query:  137 ITPARFEDPYLHLFKK*IEPDQKCLTDAKMDKSTV 241
            +T A+FE     L  + +EP +K L DA + KS +
Sbjct:  302 VTRAKFESLVSDLVMRSLEPCKKALEDAGLSKSDI 336

[Close]

BLAST of EY711022 vs. ExPASy Swiss-Prot
Match: DNAK_PROVI (Chaperone protein dnaK OS=Prosthecochloris vibrioformis (strain DSM 265) (strain DSM 265) GN=dnaK PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 7.777e-12
Identity = 29/73 (39.73%), Postives = 42/73 (57.53%), Query Frame = 1

Query:  508 PPKKEQAFSTYSDYRPEVLIHSYLAETTLTMDHELLR*FDISDITTCPNEDPQITVSLHIDDHVILHVSAEDR 726
            P KK++ FST  D +  V +H    E ++  D++ L  F + DI   P   PQI V+  ID + ILHVSA+D+
Sbjct:  412 PTKKQEVFSTAGDNQTSVEVHVLQGERSMATDNKTLGRFHLGDIPPAPRGLPQIEVAFDIDSNGILHVSAKDK 484

HSP 2 Score: 30.0314 bits (66), Expect = 7.777e-12
Identity = 30/87 (34.48%), Postives = 38/87 (43.68%), Query Frame = 3

Query:  252 LLVGVSTRIPKGLHLLRHFFYAKDLCPILTPXXXXXXXXXXXXXILI*NGNYNDHDQLLLYGTPLPHDLNTARGHMTVLIPKKQPSP 512
            +LVG STRIPK   L++ FF  K+    + P             +L   G+    D LLL  TPL   + T  G MT LI      P
Sbjct:  331 VLVGGSTRIPKVQALVKDFF-GKEPNKSVNPDEVVAVGAAIQGGVL--KGDVT--DVLLLDVTPLSLGIETLGGVMTKLIDANTTIP 412

HSP 3 Score: 24.2534 bits (51), Expect = 7.777e-12
Identity = 21/79 (26.58%), Postives = 36/79 (45.57%), Query Frame = 2

Query:    2 DITFHTIPLKILMTACERLNKTLSSTALTALEIDSLYQGID----CYSTITPARFEDPYLHLFKK*IEPDQKCLTDAKM 226
            D+    I L+ L  A E+    LSS   T + +  +    +        +T A+FE     LF K +EP Q+ + ++K+
Sbjct:  244 DLRNDPIALQRLKEAGEKAKIELSSRTDTEINLPFITATQEGPKHLVINLTRAKFEGICADLFAKILEPCQRAVKNSKV 322

[Close]

BLAST of EY711022 vs. ExPASy Swiss-Prot
Match: DNAK_FRATT (Chaperone protein dnaK OS=Francisella tularensis subsp. tularensis GN=dnaK PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 1.004e-11
Identity = 31/73 (42.47%), Postives = 42/73 (57.53%), Query Frame = 1

Query:  508 PPKKEQAFSTYSDYRPEVLIHSYLAETTLTMDHELLR*FDISDITTCPNEDPQITVSLHIDDHVILHVSAEDR 726
            P KK Q FST  D +P V IH    E  +   ++ L  FD++DI   P   PQI V+  ID + IL+VSA+D+
Sbjct:  421 PTKKSQVFSTAEDNQPAVTIHVLQGEREMASANKSLGRFDLADIPPAPRGMPQIEVTFDIDANGILNVSAKDK 493

HSP 2 Score: 27.335 bits (59), Expect = 1.004e-11
Identity = 29/95 (30.53%), Postives = 40/95 (42.11%), Query Frame = 3

Query:  228 TKALCTHALLVGVSTRIPKGLHLLRHFFYAKDLCPILTPXXXXXXXXXXXXXILI*NGNYNDHDQLLLYGTPLPHDLNTARGHMTVLIPKKQPSP 512
            +K+  T  LLVG  TR+P     ++ FF  K+    + P             +L   G+  D   LLL  TPL   + T  G MT LI +    P
Sbjct:  332 SKSDITEVLLVGGQTRMPLVQEKVKEFF-GKEPRKDVNPDEAVAVGAAIQGGVLA--GDVKDI--LLLDVTPLSLGIETMGGVMTKLIERNTTIP 421

HSP 3 Score: 26.5646 bits (57), Expect = 1.004e-11
Identity = 13/35 (37.14%), Postives = 20/35 (57.14%), Query Frame = 2

Query:  137 ITPARFEDPYLHLFKK*IEPDQKCLTDAKMDKSTV 241
            +T A+FE     L  + +EP +K L DA + KS +
Sbjct:  302 VTRAKFESLVSDLVMRSLEPCKKALEDAGLSKSDI 336

[Close]

BLAST of EY711022 vs. ExPASy Swiss-Prot
Match: DNAK_FRAT1 (Chaperone protein dnaK OS=Francisella tularensis subsp. tularensis (strain FSC 198) GN=dnaK PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 1.004e-11
Identity = 31/73 (42.47%), Postives = 42/73 (57.53%), Query Frame = 1

Query:  508 PPKKEQAFSTYSDYRPEVLIHSYLAETTLTMDHELLR*FDISDITTCPNEDPQITVSLHIDDHVILHVSAEDR 726
            P KK Q FST  D +P V IH    E  +   ++ L  FD++DI   P   PQI V+  ID + IL+VSA+D+
Sbjct:  421 PTKKSQVFSTAEDNQPAVTIHVLQGEREMASANKSLGRFDLADIPPAPRGMPQIEVTFDIDANGILNVSAKDK 493

HSP 2 Score: 27.335 bits (59), Expect = 1.004e-11
Identity = 29/95 (30.53%), Postives = 40/95 (42.11%), Query Frame = 3

Query:  228 TKALCTHALLVGVSTRIPKGLHLLRHFFYAKDLCPILTPXXXXXXXXXXXXXILI*NGNYNDHDQLLLYGTPLPHDLNTARGHMTVLIPKKQPSP 512
            +K+  T  LLVG  TR+P     ++ FF  K+    + P             +L   G+  D   LLL  TPL   + T  G MT LI +    P
Sbjct:  332 SKSDITEVLLVGGQTRMPLVQEKVKEFF-GKEPRKDVNPDEAVAVGAAIQGGVLA--GDVKDI--LLLDVTPLSLGIETMGGVMTKLIERNTTIP 421

HSP 3 Score: 26.5646 bits (57), Expect = 1.004e-11
Identity = 13/35 (37.14%), Postives = 20/35 (57.14%), Query Frame = 2

Query:  137 ITPARFEDPYLHLFKK*IEPDQKCLTDAKMDKSTV 241
            +T A+FE     L  + +EP +K L DA + KS +
Sbjct:  302 VTRAKFESLVSDLVMRSLEPCKKALEDAGLSKSDI 336

[Close]

BLAST of EY711022 vs. ExPASy Swiss-Prot
Match: DNAK_THIDA (Chaperone protein dnaK OS=Thiobacillus denitrificans (strain ATCC 25259) GN=dnaK PE=2 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 1.676e-11
Identity = 29/73 (39.73%), Postives = 40/73 (54.79%), Query Frame = 1

Query:  508 PPKKEQAFSTYSDYRPEVLIHSYLAETTLTMDHELLR*FDISDITTCPNEDPQITVSLHIDDHVILHVSAEDR 726
            P K  Q FST  D +  V +H    E  +   ++ L  F++SDI   P   PQI V+  ID + ILHVSA+D+
Sbjct:  419 PTKASQVFSTADDNQSAVTVHVLQGEREMASGNKSLGQFNLSDIPPAPRGMPQIEVTFDIDANGILHVSAKDK 491

HSP 2 Score: 32.3426 bits (72), Expect = 1.676e-11
Identity = 29/91 (31.87%), Postives = 40/91 (43.96%), Query Frame = 3

Query:  252 LLVGVSTRIPKGLHLLRHFFYAKDLCPILTPXXXXXXXXXXXXXILI*NGNYNDHDQLLLYGTPLPHDLNTARGHMTVLIPKKQPSPQEGT 524
            +LVG  TR+PK +  ++ FF  K+    + P             +L   G   D   LLL  TPL   + T  G MT LIPK    P + +
Sbjct:  338 ILVGGQTRMPKVMDAVKDFF-GKEPRRDVNPDEAVAVGAAIQGGVL--QGEVKD--VLLLDVTPLSLGIETLGGVMTKLIPKNTTIPTKAS 423

HSP 3 Score: 24.2534 bits (51), Expect = 1.676e-11
Identity = 25/86 (29.07%), Postives = 36/86 (41.86%), Query Frame = 2

Query:    2 DITFHTIPLKILMTACERLNKTLSSTALTALEIDSLYQGIDCYST------ITPARFEDPYLHLFKK*IEPDQKCLTDAKMDKSTV 241
            D+    + L+ L  A E+    LSS   T  E++  Y   D          IT A+FE     L  + IEP +  L DA +  S +
Sbjct:  251 DLKKDVLALQRLKEAAEKAKIELSSGQQT--EVNLPYITADASGPKHLAVKITRAKFESLVEELITRTIEPCKLALKDAGLTTSQI 334

[Close]

BLAST of EY711022 vs. ExPASy Swiss-Prot
Match: DNAK_CHLP8 (Chaperone protein dnaK OS=Chlorobaculum parvum (strain NCIB 8327) GN=dnaK PE=2 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 2.165e-11
Identity = 27/73 (36.99%), Postives = 42/73 (57.53%), Query Frame = 1

Query:  508 PPKKEQAFSTYSDYRPEVLIHSYLAETTLTMDHELLR*FDISDITTCPNEDPQITVSLHIDDHVILHVSAEDR 726
            P +K++ FST +D +  V +H    E  +  D++ L  F + DI   P   PQI V+  ID + IL+VSA+D+
Sbjct:  412 PTRKQETFSTAADNQTSVEVHVLQGERPMASDNKTLGRFHLGDIPPAPRGIPQIEVTFDIDANGILNVSAKDK 484

HSP 2 Score: 29.261 bits (64), Expect = 2.165e-11
Identity = 29/87 (33.33%), Postives = 38/87 (43.68%), Query Frame = 3

Query:  252 LLVGVSTRIPKGLHLLRHFFYAKDLCPILTPXXXXXXXXXXXXXILI*NGNYNDHDQLLLYGTPLPHDLNTARGHMTVLIPKKQPSP 512
            +LVG S+RIPK   L++ FF  K+    + P             +L   G+    D LLL  TPL   + T  G MT LI      P
Sbjct:  331 VLVGGSSRIPKVQALVKEFF-GKEPNKSVNPDEVVAIGAAIQGGVL--QGDVT--DVLLLDVTPLSLGIETLGGVMTKLIEANSTIP 412

HSP 3 Score: 28.1054 bits (61), Expect = 2.165e-11
Identity = 22/84 (26.19%), Postives = 38/84 (45.24%), Query Frame = 2

Query:    2 DITFHTIPLKILMTACERLNKTLSSTALTALEIDSLYQGID----CYSTITPARFEDPYLHLFKK*IEPDQKCLTDAKMDKSTV 241
            D+    I L+ L  A E+    LSS   T + +  +    +        +T A+FE     LF K +EP ++ L ++K+D   +
Sbjct:  244 DLRKDAIALQRLKEAAEKAKIELSSRTDTEINLPFITATQEGPKHLVINLTRAKFEAMSATLFDKVLEPCRRALKNSKLDMKEI 327

[Close]

BLAST of EY711022 vs. ExPASy Swiss-Prot
Match: DNAK_CHLTE (Chaperone protein dnaK OS=Chlorobium tepidum GN=dnaK PE=2 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 2.797e-11
Identity = 28/73 (38.36%), Postives = 43/73 (58.90%), Query Frame = 1

Query:  508 PPKKEQAFSTYSDYRPEVLIHSYLAETTLTMDHELLR*FDISDITTCPNEDPQITVSLHIDDHVILHVSAEDR 726
            P +K++ FST +D +  V +H    E  +  D++ L  F +SDI   P   PQI V+  ID + IL+VSA+D+
Sbjct:  412 PTRKQEIFSTAADNQTSVEVHVLQGERPMASDNKTLGRFHLSDIPPAPRGVPQIEVTFDIDANGILNVSAKDK 484

HSP 2 Score: 30.4166 bits (67), Expect = 2.797e-11
Identity = 30/87 (34.48%), Postives = 38/87 (43.68%), Query Frame = 3

Query:  252 LLVGVSTRIPKGLHLLRHFFYAKDLCPILTPXXXXXXXXXXXXXILI*NGNYNDHDQLLLYGTPLPHDLNTARGHMTVLIPKKQPSP 512
            +LVG STRIPK   L++ FF  K+    + P             +L   G+    D LLL  TPL   + T  G MT LI      P
Sbjct:  331 VLVGGSTRIPKVQALVKEFF-GKEPNKSVNPDEVVAIGAAIQGGVL--QGDVT--DVLLLDVTPLSLGIETLGGVMTKLIEANTTIP 412

HSP 3 Score: 25.409 bits (54), Expect = 2.797e-11
Identity = 21/80 (26.25%), Postives = 35/80 (43.75%), Query Frame = 2

Query:    2 DITFHTIPLKILMTACERLNKTLSSTALTALEIDSLYQGID----CYSTITPARFEDPYLHLFKK*IEPDQKCLTDAKMD 229
            D+    I L+ L  A E+    LSS   T + +  +    +        +T A+FE     LF K  EP ++ + ++K D
Sbjct:  244 DLRKDAIALQRLKEAAEKAKIELSSRTDTEINLPFITATQEGPKHLVINLTRAKFEAMSAALFDKLFEPCRRAIKNSKFD 323

[Close]

BLAST of EY711022 vs. ExPASy Swiss-Prot
Match: DNAK_PELLD (Chaperone protein dnaK OS=Pelodictyon luteolum (strain DSM 273) GN=dnaK PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 3.611e-11
Identity = 28/73 (38.36%), Postives = 42/73 (57.53%), Query Frame = 1

Query:  508 PPKKEQAFSTYSDYRPEVLIHSYLAETTLTMDHELLR*FDISDITTCPNEDPQITVSLHIDDHVILHVSAEDR 726
            P +K++ FST  D +  V +H    E  L +D++ L  F + DI   P   PQI V+  ID + IL+VSA+D+
Sbjct:  412 PTRKQEVFSTAGDNQTSVEVHVLQGERPLAVDNKTLGRFHLGDIPPAPRGMPQIEVTFDIDSNGILNVSAKDK 484

HSP 2 Score: 30.0314 bits (66), Expect = 3.611e-11
Identity = 30/87 (34.48%), Postives = 38/87 (43.68%), Query Frame = 3

Query:  252 LLVGVSTRIPKGLHLLRHFFYAKDLCPILTPXXXXXXXXXXXXXILI*NGNYNDHDQLLLYGTPLPHDLNTARGHMTVLIPKKQPSP 512
            +LVG STRIPK   L++ FF  K+    + P             +L   G+    D LLL  TPL   + T  G MT LI      P
Sbjct:  331 VLVGGSTRIPKVQALVKEFF-GKEPNRSVNPDEVVAIGAAIQGGVL--KGDVT--DVLLLDVTPLSLGIETLGGVMTKLIDANTTIP 412

HSP 3 Score: 24.6386 bits (52), Expect = 3.611e-11
Identity = 20/84 (23.81%), Postives = 38/84 (45.24%), Query Frame = 2

Query:    2 DITFHTIPLKILMTACERLNKTLSSTALTALEIDSLYQGID----CYSTITPARFEDPYLHLFKK*IEPDQKCLTDAKMDKSTV 241
            D+    I L+ L  A E+    LSS   T + +  +    +        +T A+FE     LF K +EP ++ + ++K++   +
Sbjct:  244 DLRNDAIALQRLKEAGEKAKVELSSRTDTEINLPFITATPEGPKHLVINLTRAKFEGMCSDLFDKILEPCRRAVKNSKVEMKDI 327

[Close]

BLAST of EY711022 vs. ExPASy Swiss-Prot
Match: DNAK_CHLL2 (Chaperone protein dnaK OS=Chlorobium limicola (strain DSM 245 / NBRC 103803) GN=dnaK PE=2 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 4.663e-11
Identity = 27/73 (36.99%), Postives = 41/73 (56.16%), Query Frame = 1

Query:  508 PPKKEQAFSTYSDYRPEVLIHSYLAETTLTMDHELLR*FDISDITTCPNEDPQITVSLHIDDHVILHVSAEDR 726
            P +K++ FST +D +  V +H    E  +  D++ L  F + DI   P   PQI V+  ID + IL VSA+D+
Sbjct:  412 PTRKQEVFSTAADSQTSVEVHVLQGERPMASDNKTLGRFHLGDIPPAPRGVPQIEVTFDIDSNGILSVSAKDK 484

HSP 2 Score: 28.1054 bits (61), Expect = 4.663e-11
Identity = 21/84 (25.00%), Postives = 38/84 (45.24%), Query Frame = 2

Query:    2 DITFHTIPLKILMTACERLNKTLSSTALTALEIDSLYQGID----CYSTITPARFEDPYLHLFKK*IEPDQKCLTDAKMDKSTV 241
            D+    I L+ L  A E+    LSS   T + +  +    +        +T A+FE     LF+K +EP  + + ++K+D   +
Sbjct:  244 DLRNDAIALQRLKEAAEKAKVELSSRTDTEINLPFITATQEGPKHLVINLTRAKFEAMCADLFEKILEPCHRAVKNSKLDMKEI 327

HSP 3 Score: 28.1054 bits (61), Expect = 4.663e-11
Identity = 29/87 (33.33%), Postives = 38/87 (43.68%), Query Frame = 3

Query:  252 LLVGVSTRIPKGLHLLRHFFYAKDLCPILTPXXXXXXXXXXXXXILI*NGNYNDHDQLLLYGTPLPHDLNTARGHMTVLIPKKQPSP 512
            +LVG STRIPK   L++ FF  K+    + P             +L   G+    D LLL  +PL   + T  G MT LI      P
Sbjct:  331 VLVGGSTRIPKVQTLVKEFF-GKEPNRSVNPDEVVAIGAAIQGGVL--KGDVT--DVLLLDVSPLSLGIETLGGVMTRLIEANTTIP 412

The following BLAST results are available for this feature:

BLAST of EY711022 vs. ExPASy Swiss-Prot

BLAST of EY711022 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 189


Match Name	E-value	Identity	Description
DNAK_FRAP2	7.773e-12	42.47	Chaperone protein dnaK OS=Francisella philomiragia...	[more]
DNAK_PROVI	7.777e-12	39.73	Chaperone protein dnaK OS=Prosthecochloris vibriof...	[more]
DNAK_FRATT	1.004e-11	42.47	Chaperone protein dnaK OS=Francisella tularensis s...	[more]
DNAK_FRAT1	1.004e-11	42.47	Chaperone protein dnaK OS=Francisella tularensis s...	[more]
DNAK_THIDA	1.676e-11	39.73	Chaperone protein dnaK OS=Thiobacillus denitrifica...	[more]
DNAK_CHLP8	2.165e-11	36.99	Chaperone protein dnaK OS=Chlorobaculum parvum (st...	[more]
DNAK_CHLTE	2.797e-11	38.36	Chaperone protein dnaK OS=Chlorobium tepidum GN=dn...	[more]
DNAK_PELLD	3.611e-11	38.36	Chaperone protein dnaK OS=Pelodictyon luteolum (st...	[more]
DNAK_CHLL2	4.663e-11	36.99	Chaperone protein dnaK OS=Chlorobium limicola (str...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-702-002-G10-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY711022 ID=EY711022; Name=EY711022; organism=Citrus sinensis; type=EST; length=886bp
GGATATTACTTTCCACACGATACCTCTTAAGATATTGATGACTGCTTGTG
AGAGACTCAACAAGACTCTCTCCTCTACTGCCCTCACTGCACTTGAGATT
GATTCTTTGTATCAGGGAATTGACTGCTACTCCACCATTACTCCTGCCAG
ATTTGAAGACCCCTATCTGCATCTCTTCAAGAAGTGAATAGAGCCTGATC
AAAAATGCTTGACGGATGCTAAGATGGACAAAAGCACTGTGCACACATGC
CCTGCTTGTGGGCGTTTCCACTAGGATTCCCAAGGGGCTACACCTTTTGC
GACACTTCTTCTATGCAAAGGACCTCTGCCCGATCCTTACTCCATATTAC
GTTGCTGCTTATGCAGCTGCTGCTCATGTTGCCATCCTGATCTAGAACGG
CAACTACAATGATCATGATCAGTTGCTCCTGTATGGCACACCTCTGCCTC
ATGACTTGAATACTGCTAGGGGTCACATGACTGTGCTGATCCCAAAAAAA
CAACCATCCCCCCAAGAAGGAACAAGCTTTCTCCACCTACTCAGACTACC
GACCCGAAGTGCTGATACACTCGTACCTAGCTGAGACGACTCTAACCATG
GATCACGAATTGTTGCGATAATTTGATATCTCTGACATTACTACCTGTCC
CAACGAAGATCCTCAGATCACTGTATCGCTTCATATCGATGATCATGTGA
TCTTGCATGTCTCAGCAGAAGATAGACGACCCGCCATTAGATCATGATCC
TATCACCATCGAATATGATCACGTCATGAGACGAACTCAGAAAATGGTTT
AGATGCCTTGAGTACGAAGCTGATGACAGTACCCTTTACGAGGCCCCGTC
ATCACTGCTTGCAAATTTGCCTTCATGTGATGATCC