EY710867

Overview
NameEY710867
Unique NameEY710867
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length753
Libraries
Library NameType
Sweet orange fruit, development stadium (3 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig642 contig Csv1_Contig642:305..1056. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY710867 vs. ExPASy Swiss-Prot
Match: FRMA_SHISS (S-(hydroxymethyl)glutathione dehydrogenase OS=Shigella sonnei (strain Ss046) GN=frmA PE=3 SV=1)

HSP 1 Score: 288.115 bits (736), Expect = 3.461e-77
Identity = 140/244 (57.38%), Postives = 172/244 (70.49%), Query Frame = 1 
Query:   13 KILFTALCHTDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRGATGAGVMMNDRKSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPQAPLDKVCLLGCGVPTGLGAVWNTAKVEPGSIVAVFGLGTVXXXXXXXXXXXXXSRVIGIDMDPKKFDRAKNFGVPEFVNPKDHDNPFSRSLSISRMXGGDYIFECICNVSGMKAAFE 744
            K+  T +CHTDA+T SG DPEG+FP +LGHE AG+V  VGEGVT V+PGDHVIP Y AEC EC+FC+SGKTNLC  VR   G G +M D  +RFS NG+P+YH+MG STFS+YTVV +VS+AKI+P+A  + VCLLGCGV TG+GAV NTAKV+PG  VAVFGLG +              R+I ID +PKKFD A+ FG  + +NP D+D P    L      G D+ FECI NV+ M+AA E
Sbjct:   33 KVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKG-LMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALE 275
[Close]
BLAST of EY710867 vs. ExPASy Swiss-Prot
Match: FRMA_ECOLI (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli (strain K12) GN=frmA PE=1 SV=3)

HSP 1 Score: 288.115 bits (736), Expect = 3.461e-77
Identity = 140/244 (57.38%), Postives = 172/244 (70.49%), Query Frame = 1 
Query:   13 KILFTALCHTDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRGATGAGVMMNDRKSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPQAPLDKVCLLGCGVPTGLGAVWNTAKVEPGSIVAVFGLGTVXXXXXXXXXXXXXSRVIGIDMDPKKFDRAKNFGVPEFVNPKDHDNPFSRSLSISRMXGGDYIFECICNVSGMKAAFE 744
            K+  T +CHTDA+T SG DPEG+FP +LGHE AG+V  VGEGVT V+PGDHVIP Y AEC EC+FC+SGKTNLC  VR   G G +M D  +RFS NG+P+YH+MG STFS+YTVV +VS+AKI+P+A  + VCLLGCGV TG+GAV NTAKV+PG  VAVFGLG +              R+I ID +PKKFD A+ FG  + +NP D+D P    L      G D+ FECI NV+ M+AA E
Sbjct:   33 KVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKG-LMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALE 275
[Close]
BLAST of EY710867 vs. ExPASy Swiss-Prot
Match: FRMA_ECOLC (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=frmA PE=3 SV=1)

HSP 1 Score: 288.115 bits (736), Expect = 3.461e-77
Identity = 140/244 (57.38%), Postives = 172/244 (70.49%), Query Frame = 1 
Query:   13 KILFTALCHTDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRGATGAGVMMNDRKSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPQAPLDKVCLLGCGVPTGLGAVWNTAKVEPGSIVAVFGLGTVXXXXXXXXXXXXXSRVIGIDMDPKKFDRAKNFGVPEFVNPKDHDNPFSRSLSISRMXGGDYIFECICNVSGMKAAFE 744
            K+  T +CHTDA+T SG DPEG+FP +LGHE AG+V  VGEGVT V+PGDHVIP Y AEC EC+FC+SGKTNLC  VR   G G +M D  +RFS NG+P+YH+MG STFS+YTVV +VS+AKI+P+A  + VCLLGCGV TG+GAV NTAKV+PG  VAVFGLG +              R+I ID +PKKFD A+ FG  + +NP D+D P    L      G D+ FECI NV+ M+AA E
Sbjct:   33 KVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKG-LMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALE 275
[Close]
BLAST of EY710867 vs. ExPASy Swiss-Prot
Match: FRMA_ECOHS (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli O9:H4 (strain HS) GN=frmA PE=3 SV=1)

HSP 1 Score: 288.115 bits (736), Expect = 3.461e-77
Identity = 140/244 (57.38%), Postives = 172/244 (70.49%), Query Frame = 1 
Query:   13 KILFTALCHTDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRGATGAGVMMNDRKSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPQAPLDKVCLLGCGVPTGLGAVWNTAKVEPGSIVAVFGLGTVXXXXXXXXXXXXXSRVIGIDMDPKKFDRAKNFGVPEFVNPKDHDNPFSRSLSISRMXGGDYIFECICNVSGMKAAFE 744
            K+  T +CHTDA+T SG DPEG+FP +LGHE AG+V  VGEGVT V+PGDHVIP Y AEC EC+FC+SGKTNLC  VR   G G +M D  +RFS NG+P+YH+MG STFS+YTVV +VS+AKI+P+A  + VCLLGCGV TG+GAV NTAKV+PG  VAVFGLG +              R+I ID +PKKFD A+ FG  + +NP D+D P    L      G D+ FECI NV+ M+AA E
Sbjct:   33 KVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKG-LMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALE 275
[Close]
BLAST of EY710867 vs. ExPASy Swiss-Prot
Match: FRMA_ECO57 (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli O157:H7 GN=frmA PE=3 SV=1)

HSP 1 Score: 288.115 bits (736), Expect = 3.461e-77
Identity = 140/244 (57.38%), Postives = 172/244 (70.49%), Query Frame = 1 
Query:   13 KILFTALCHTDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRGATGAGVMMNDRKSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPQAPLDKVCLLGCGVPTGLGAVWNTAKVEPGSIVAVFGLGTVXXXXXXXXXXXXXSRVIGIDMDPKKFDRAKNFGVPEFVNPKDHDNPFSRSLSISRMXGGDYIFECICNVSGMKAAFE 744
            K+  T +CHTDA+T SG DPEG+FP +LGHE AG+V  VGEGVT V+PGDHVIP Y AEC EC+FC+SGKTNLC  VR   G G +M D  +RFS NG+P+YH+MG STFS+YTVV +VS+AKI+P+A  + VCLLGCGV TG+GAV NTAKV+PG  VAVFGLG +              R+I ID +PKKFD A+ FG  + +NP D+D P    L      G D+ FECI NV+ M+AA E
Sbjct:   33 KVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKG-LMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALE 275
[Close]
BLAST of EY710867 vs. ExPASy Swiss-Prot
Match: FRMA_ECO24 (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=frmA PE=3 SV=1)

HSP 1 Score: 288.115 bits (736), Expect = 3.461e-77
Identity = 140/244 (57.38%), Postives = 172/244 (70.49%), Query Frame = 1 
Query:   13 KILFTALCHTDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRGATGAGVMMNDRKSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPQAPLDKVCLLGCGVPTGLGAVWNTAKVEPGSIVAVFGLGTVXXXXXXXXXXXXXSRVIGIDMDPKKFDRAKNFGVPEFVNPKDHDNPFSRSLSISRMXGGDYIFECICNVSGMKAAFE 744
            K+  T +CHTDA+T SG DPEG+FP +LGHE AG+V  VGEGVT V+PGDHVIP Y AEC EC+FC+SGKTNLC  VR   G G +M D  +RFS NG+P+YH+MG STFS+YTVV +VS+AKI+P+A  + VCLLGCGV TG+GAV NTAKV+PG  VAVFGLG +              R+I ID +PKKFD A+ FG  + +NP D+D P    L      G D+ FECI NV+ M+AA E
Sbjct:   33 KVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKG-LMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALE 275
[Close]
BLAST of EY710867 vs. ExPASy Swiss-Prot
Match: ADH1_ORYSJ (Alcohol dehydrogenase 1 OS=Oryza sativa subsp. japonica GN=ADH1 PE=2 SV=2)

HSP 1 Score: 288.115 bits (736), Expect = 3.461e-77
Identity = 139/246 (56.50%), Postives = 169/246 (68.70%), Query Frame = 1 
Query:   13 KILFTALCHTDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRGATGAGVMMNDRKSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPQAPLDKVCLLGCGVPTGLGAVWNTAKVEPGSIVAVFGLGTVXXXXXXXXXXXXXSRVIGIDMDPKKFDRAKNFGVPEFVNPKDHDNPFSRSLSISRMXGGDYIFECICNVSGMKAAFECL 750
            KILFT+LCHTD Y W  K    +FP I GHEA GIVESVGEGVT++ PGDHV+P +  EC+EC  CKS ++N+C  +R  T  GVM+ D KSRFSINGKPIYHF+GTSTFS+YTV+H   VAKI+P APLDKVC+L CG+ TGLGA  N AK   GS VA+FGLG V             SR+IGID++  +F+ A+ FG  EFVNPKDHD P  + L+     G D   EC  N++ M  AFEC+
Sbjct:   40 KILFTSLCHTDVYFWEAKGQTPVFPRIFGHEAGGIVESVGEGVTDLAPGDHVLPVFTGECKECAHCKSAESNMCDLLRINTDRGVMIGDGKSRFSINGKPIYHFVGTSTFSEYTVMHVGCVAKINPAAPLDKVCVLSCGISTGLGATINVAKPPKGSTVAIFGLGAVGLAAAEGARIAGASRIIGIDLNANRFEEARKFGCTEFVNPKDHDKPVQQVLAEMTNGGVDRSVECTGNINAMIQAFECV 285
[Close]
BLAST of EY710867 vs. ExPASy Swiss-Prot
Match: ADH1_ORYSI (Alcohol dehydrogenase 1 OS=Oryza sativa subsp. indica GN=ADH1 PE=2 SV=1)

HSP 1 Score: 288.115 bits (736), Expect = 3.461e-77
Identity = 139/246 (56.50%), Postives = 169/246 (68.70%), Query Frame = 1 
Query:   13 KILFTALCHTDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRGATGAGVMMNDRKSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPQAPLDKVCLLGCGVPTGLGAVWNTAKVEPGSIVAVFGLGTVXXXXXXXXXXXXXSRVIGIDMDPKKFDRAKNFGVPEFVNPKDHDNPFSRSLSISRMXGGDYIFECICNVSGMKAAFECL 750
            KILFT+LCHTD Y W  K    +FP I GHEA GIVESVGEGVT++ PGDHV+P +  EC+EC  CKS ++N+C  +R  T  GVM+ D KSRFSINGKPIYHF+GTSTFS+YTV+H   VAKI+P APLDKVC+L CG+ TGLGA  N AK   GS VA+FGLG V             SR+IGID++  +F+ A+ FG  EFVNPKDHD P  + L+     G D   EC  N++ M  AFEC+
Sbjct:   40 KILFTSLCHTDVYFWEAKGQTPVFPRIFGHEAGGIVESVGEGVTDLAPGDHVLPVFTGECKECAHCKSAESNMCDLLRINTDRGVMIGDGKSRFSINGKPIYHFVGTSTFSEYTVMHVGCVAKINPAAPLDKVCVLSCGISTGLGATINVAKPPKGSTVAIFGLGAVGLAAAEGARIAGASRIIGIDLNANRFEEARKFGCTEFVNPKDHDKPVQQVLAEMTNGGVDRSVECTGNINAMIQAFECV 285
[Close]
BLAST of EY710867 vs. ExPASy Swiss-Prot
Match: ADH3_SOLTU (Alcohol dehydrogenase 3 OS=Solanum tuberosum GN=ADH3 PE=2 SV=1)

HSP 1 Score: 286.96 bits (733), Expect = 7.711e-77
Identity = 138/246 (56.10%), Postives = 172/246 (69.92%), Query Frame = 1 
Query:   13 KILFTALCHTDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRGATGAGVMMNDRKSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPQAPLDKVCLLGCGVPTGLGAVWNTAKVEPGSIVAVFGLGTVXXXXXXXXXXXXXSRVIGIDMDPKKFDRAKNFGVPEFVNPKDHDNPFSRSLSISRMXGGDYIFECICNVSGMKAAFECL 750
            KIL+T+LCHTD Y W  K    +FP ILGHEAAGIVESVGEGVTE+ PGDHV+P +  EC++C  CKS ++N+C  +R  T  GVM+ND +SRFSINGKPIYHF+GTSTFS+YTVVH   VAKI+P APLDKVC+L CG+ TGLGA  N AK   GS VA+FGLG V             SR+IG+D++  +F++AK FGV EFVNPKD+  P    ++     G D   EC  ++  M +AFEC+
Sbjct:   41 KILYTSLCHTDVYFWEAKGQNPVFPRILGHEAAGIVESVGEGVTELAPGDHVLPVFTGECKDCAHCKSEESNMCSLLRINTDRGVMINDGQSRFSINGKPIYHFVGTSTFSEYTVVHVGCVAKINPLAPLDKVCVLSCGISTGLGATLNVAKPTKGSSVAIFGLGAVGLAAAEGARIAGASRIIGVDLNASRFEQAKKFGVTEFVNPKDYSKPVQEVIAEMTDGGVDRSVECTGHIDAMISAFECV 286
[Close]
BLAST of EY710867 vs. ExPASy Swiss-Prot
Match: ADH2_SOLTU (Alcohol dehydrogenase 2 OS=Solanum tuberosum GN=ADH2 PE=2 SV=1)

HSP 1 Score: 286.96 bits (733), Expect = 7.711e-77
Identity = 138/246 (56.10%), Postives = 172/246 (69.92%), Query Frame = 1 
Query:   13 KILFTALCHTDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRGATGAGVMMNDRKSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPQAPLDKVCLLGCGVPTGLGAVWNTAKVEPGSIVAVFGLGTVXXXXXXXXXXXXXSRVIGIDMDPKKFDRAKNFGVPEFVNPKDHDNPFSRSLSISRMXGGDYIFECICNVSGMKAAFECL 750
            KIL+T+LCHTD Y W  K    +FP ILGHEAAGIVESVGEGVTE+ PGDHV+P +  EC++C  CKS ++N+C  +R  T  GVM+ND +SRFSINGKPIYHF+GTSTFS+YTVVH   VAKI+P APLDKVC+L CG+ TGLGA  N AK   GS VA+FGLG V             SR+IG+D++  +F++AK FGV EFVNPKD+  P    ++     G D   EC  ++  M +AFEC+
Sbjct:   41 KILYTSLCHTDVYFWEAKGQNPVFPRILGHEAAGIVESVGEGVTELAPGDHVLPVFTGECKDCAHCKSEESNMCSLLRINTDRGVMINDGQSRFSINGKPIYHFVGTSTFSEYTVVHVGCVAKINPLAPLDKVCVLSCGISTGLGATLNVAKPTKGSSVAIFGLGAVGLAAAEGARIAGASRIIGVDLNASRFEQAKKFGVTEFVNPKDYSKPVQEVIAEMTDGGVDRSVECTGHIDAMISAFECV 286
The following BLAST results are available for this feature:
BLAST of EY710867 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 154
Match NameE-valueIdentityDescription
FRMA_SHISS3.461e-7757.38S-(hydroxymethyl)glutathione dehydrogenase OS=Shig... [more]
FRMA_ECOLI3.461e-7757.38S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]
FRMA_ECOLC3.461e-7757.38S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]
FRMA_ECOHS3.461e-7757.38S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]
FRMA_ECO573.461e-7757.38S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]
FRMA_ECO243.461e-7757.38S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]
ADH1_ORYSJ3.461e-7756.50Alcohol dehydrogenase 1 OS=Oryza sativa subsp. jap... [more]
ADH1_ORYSI3.461e-7756.50Alcohol dehydrogenase 1 OS=Oryza sativa subsp. ind... [more]
ADH3_SOLTU7.711e-7756.10Alcohol dehydrogenase 3 OS=Solanum tuberosum GN=AD... [more]
ADH2_SOLTU7.711e-7756.10Alcohol dehydrogenase 2 OS=Solanum tuberosum GN=AD... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-702-001-B05-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY710867 ID=EY710867; Name=EY710867; organism=Citrus sinensis; type=EST; length=753bp
CCACGCGTCCGCAAGATTCTCTTCACTGCTCTCTGCCACACTGATGCTTA
CACCTGGAGCGGCAAGGATCCTGAAGGTCTCTTCCCTTGTATCCTAGGTC
ATGAGGCTGCTGGGATTGTGGAGAGTGTTGGTGAAGGTGTGACCGAGGTT
CAACCAGGTGATCATGTCATCCCTTGTTACCAGGCAGAATGCCGAGAGTG
CAAGTTTTGCAAGTCTGGAAAGACTAACCTCTGTGGTAAGGTTAGGGGTG
CCACTGGTGCTGGTGTCATGATGAATGACCGCAAGAGCCGTTTCTCGATA
AATGGAAAGCCTATCTATCACTTCATGGGAACCTCAACATTTAGCCAGTA
CACAGTTGTGCATGATGTTAGTGTTGCAAAGATTGACCCACAAGCTCCTT
TGGACAAAGTATGCCTTCTTGGCTGTGGTGTTCCTACTGGTCTTGGAGCA
GTTTGGAACACTGCAAAAGTAGAGCCGGGTTCTATAGTTGCTGTTTTTGG
CCTTGGGACTGTTGGCCTTGCTGTGGCAGAGGGTGCAAAGGCAGCTGGAG
CTTCACGTGTTATTGGTATAGATATGGACCCAAAAAAGTTTGATAGAGCA
AAGAATTTTGGAGTTCCTGAATTTGTAAATCCCAAGGACCATGACAACCC
ATTCAGCAGGTCTTTGTCAATCTCACGGATGGNNGGGGGTGACTATATTT
TTGAATGCATTTGCAATGTCTCTGGGATGAAGGCAGCTTTTGAATGCTTG
TCA
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