EY710860

Overview
NameEY710860
Unique NameEY710860
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length915
Libraries
Library NameType
Sweet orange fruit, development stadium (3 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_SHEB8 (NAD-dependent malic enzyme OS=Shewanella baltica (strain OS185) GN=sfcA PE=3 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 5.184e-40
Identity = 79/178 (44.38%), Postives = 120/178 (67.42%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  +L  P  NKG AF+E+ER    L GL+P ++ + + Q  +     R +   L K++ +  +++ NE LFY+L+ +N+ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERILGLGD G  GMGIP GKL+LYT+ GGI P+  LPI
Sbjct:   13 AGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNNISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERILGLGDQGIGGMGIPIGKLSLYTSCGGISPAYTLPI 190

HSP 2 Score: 23.0978 bits (48), Expect = 5.184e-40
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G +N QLLED  Y+G
Sbjct:  195 GTDNPQLLEDPMYMG 209
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_SHEB5 (NAD-dependent malic enzyme OS=Shewanella baltica (strain OS155 / ATCC BAA-1091) GN=sfcA PE=3 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 5.184e-40
Identity = 79/178 (44.38%), Postives = 120/178 (67.42%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  +L  P  NKG AF+E+ER    L GL+P ++ + + Q  +     R +   L K++ +  +++ NE LFY+L+ +N+ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERILGLGD G  GMGIP GKL+LYT+ GGI P+  LPI
Sbjct:   13 AGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNNISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERILGLGDQGIGGMGIPIGKLSLYTSCGGISPAYTLPI 190

HSP 2 Score: 23.0978 bits (48), Expect = 5.184e-40
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G +N QLLED  Y+G
Sbjct:  195 GTDNPQLLEDPMYMG 209
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_SHEB2 (NAD-dependent malic enzyme OS=Shewanella baltica (strain OS223) GN=sfcA PE=3 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 5.184e-40
Identity = 79/178 (44.38%), Postives = 120/178 (67.42%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  +L  P  NKG AF+E+ER    L GL+P ++ + + Q  +     R +   L K++ +  +++ NE LFY+L+ +N+ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERILGLGD G  GMGIP GKL+LYT+ GGI P+  LPI
Sbjct:   13 AGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNNISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERILGLGDQGIGGMGIPIGKLSLYTSCGGISPAYTLPI 190

HSP 2 Score: 23.0978 bits (48), Expect = 5.184e-40
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G +N QLLED  Y+G
Sbjct:  195 GTDNPQLLEDPMYMG 209
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_PROMH (NAD-dependent malic enzyme OS=Proteus mirabilis (strain HI4320) GN=sfcA PE=3 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 6.014e-40
Identity = 82/178 (46.07%), Postives = 122/178 (68.54%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+E+ER    L GLLP +V + + Q ++       ++    K++ +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+IS   +  I ++L+N  +++++VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPILLEFPLLNKGSAFSEEERSTFNLHGLLPEAVETIEEQVERAYRQYLDFKNDNDKHIYLRNIQDTNETLFYRLLESHLTEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNRANIDDMLQNATKQNVKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_PSEU2 (NAD-dependent malic enzyme OS=Pseudomonas syringae pv. syringae (strain B728a) GN=sfcA PE=3 SV=2)

HSP 1 Score: 162.54 bits (410), Expect = 8.799e-40
Identity = 87/185 (47.03%), Postives = 125/185 (67.57%), Query Frame = 2 
Query:  173 PWTISVASGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            P  IS A G  LL  P  NKG AFT +ER    L GLLP +V + + Q  ++    +Q    L K++ +  +++ NE LF++LL  +++E+LP++YTPTVG+ACQ++  I+R  +GL+IS  E+ +I ++L++  +  I++IVVTD ERILGLGD G  GMGIP GKL+LYTA GGI P+  LPI
Sbjct:    7 PLYISYA-GPSLLEMPLLNKGSAFTPQERVEFNLIGLLPQNVETIEEQVTRVYSQYKQCASDLDKHIYLRSIQDNNETLFFRLLDSHLDEMLPIIYTPTVGQACQEFSKIYRTHRGLFISYPERDRIDDILRSATKDRIKIIVVTDSERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPI 190

HSP 2 Score: 23.0978 bits (48), Expect = 8.799e-40
Identity = 8/15 (53.33%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN +LL+D  Y+G
Sbjct:  195 GTNNRELLDDPMYMG 209
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_PSESM (NAD-dependent malic enzyme OS=Pseudomonas syringae pv. tomato GN=sfcA PE=3 SV=2)

HSP 1 Score: 162.54 bits (410), Expect = 8.799e-40
Identity = 87/185 (47.03%), Postives = 125/185 (67.57%), Query Frame = 2 
Query:  173 PWTISVASGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            P  IS A G  LL  P  NKG AFT +ER    L GLLP +V + + Q  ++    +Q    L K++ +  +++ NE LF++LL  +++E+LP++YTPTVG+ACQ++  I+R  +GL+IS  E+ +I ++L++  +  I++IVVTD ERILGLGD G  GMGIP GKL+LYTA GGI P+  LPI
Sbjct:    7 PLYISYA-GPSLLEMPLLNKGSAFTPQERIEFNLIGLLPQNVETIEEQVTRVYSQYKQCASDLDKHIYLRSIQDNNETLFFRLLDSHLDEMLPIIYTPTVGQACQEFSKIYRTHRGLFISYPERDRIDDILRSATKDRIKIIVVTDSERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPI 190

HSP 2 Score: 23.0978 bits (48), Expect = 8.799e-40
Identity = 8/15 (53.33%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN +LL+D  Y+G
Sbjct:  195 GTNNRELLDDPMYMG 209
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_PSE14 (NAD-dependent malic enzyme OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=sfcA PE=3 SV=2)

HSP 1 Score: 162.54 bits (410), Expect = 8.799e-40
Identity = 87/185 (47.03%), Postives = 125/185 (67.57%), Query Frame = 2 
Query:  173 PWTISVASGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            P  IS A G  LL  P  NKG AFT +ER    L GLLP +V + + Q  ++    +Q    L K++ +  +++ NE LF++LL  +++E+LP++YTPTVG+ACQ++  I+R  +GL+IS  E+ +I ++L++  +  I++IVVTD ERILGLGD G  GMGIP GKL+LYTA GGI P+  LPI
Sbjct:    7 PLYISYA-GPSLLEMPLLNKGSAFTPQERVEFNLIGLLPQNVETIEEQVTRVYSQYKQCASDLDKHIYLRSIQDNNETLFFRLLDSHLDEMLPIIYTPTVGQACQEFSKIYRTHRGLFISYPERDRIDDILRSATKDRIKIIVVTDSERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPI 190

HSP 2 Score: 23.0978 bits (48), Expect = 8.799e-40
Identity = 8/15 (53.33%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN +LL+D  Y+G
Sbjct:  195 GTNNRELLDDPMYMG 209
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_COLP3 (NAD-dependent malic enzyme OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) GN=sfcA PE=3 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 8.799e-40
Identity = 79/178 (44.38%), Postives = 121/178 (67.98%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+++ER +  L GLLP    S   Q ++       ++  + K++ +  + + NE LF+KL+ +N+ E++P++YTPTVG+AC+++  I+R  +GL+IS +++  I ++L+N  +  ++VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   13 AGPSLLETPLLNKGSAFSKEERGSFNLTGLLPPRFESIDEQAERAFRQYSCFQTNINKHIYLRAIHDNNETLFFKLVQNNLAEMMPIIYTPTVGDACEQFSDIYRSSRGLFISYEDRFNIDDMLRNATKNKVKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAHTLPV 190

HSP 2 Score: 23.8682 bits (50), Expect = 8.799e-40
Identity = 11/26 (42.31%), Postives = 15/26 (57.69%), Query Frame = 3 
Query:  708 PQHVCPYR*-CGDNNEQLLEDDFYIG 782
            P H  P     G NN++LL+D  Y+G
Sbjct:  184 PAHTLPVMLDVGTNNQKLLDDPMYMG 209
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_SHESR (NAD-dependent malic enzyme OS=Shewanella sp. (strain MR-7) GN=sfcA PE=3 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 1.946e-39
Identity = 78/178 (43.82%), Postives = 120/178 (67.42%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  +L  P  NKG AF+E+ER    L GL+P ++ + + Q  +     R +   L K++ +  +++ NE LFY+L+ +++ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERILGLGD G  GMGIP GKL+LYT+ GGI P+  LPI
Sbjct:   13 AGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERILGLGDQGIGGMGIPIGKLSLYTSCGGISPAYTLPI 190

HSP 2 Score: 23.0978 bits (48), Expect = 1.946e-39
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G +N QLLED  Y+G
Sbjct:  195 GTDNPQLLEDPMYMG 209
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_SHESM (NAD-dependent malic enzyme OS=Shewanella sp. (strain MR-4) GN=sfcA PE=3 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 1.946e-39
Identity = 78/178 (43.82%), Postives = 120/178 (67.42%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  +L  P  NKG AF+E+ER    L GL+P ++ + + Q  +     R +   L K++ +  +++ NE LFY+L+ +++ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERILGLGD G  GMGIP GKL+LYT+ GGI P+  LPI
Sbjct:   13 AGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERILGLGDQGIGGMGIPIGKLSLYTSCGGISPAYTLPI 190

HSP 2 Score: 23.0978 bits (48), Expect = 1.946e-39
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G +N QLLED  Y+G
Sbjct:  195 GTDNPQLLEDPMYMG 209
The following BLAST results are available for this feature:
BLAST of EY710860 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAO1_SHEB85.184e-4044.38NAD-dependent malic enzyme OS=Shewanella baltica (... [more]
MAO1_SHEB55.184e-4044.38NAD-dependent malic enzyme OS=Shewanella baltica (... [more]
MAO1_SHEB25.184e-4044.38NAD-dependent malic enzyme OS=Shewanella baltica (... [more]
MAO1_PROMH6.014e-4046.07NAD-dependent malic enzyme OS=Proteus mirabilis (s... [more]
MAO1_PSEU28.799e-4047.03NAD-dependent malic enzyme OS=Pseudomonas syringae... [more]
MAO1_PSESM8.799e-4047.03NAD-dependent malic enzyme OS=Pseudomonas syringae... [more]
MAO1_PSE148.799e-4047.03NAD-dependent malic enzyme OS=Pseudomonas syringae... [more]
MAO1_COLP38.799e-4044.38NAD-dependent malic enzyme OS=Colwellia psychreryt... [more]
MAO1_SHESR1.946e-3943.82NAD-dependent malic enzyme OS=Shewanella sp. (stra... [more]
MAO1_SHESM1.946e-3943.82NAD-dependent malic enzyme OS=Shewanella sp. (stra... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-702-001-A09-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY710860 ID=EY710860; Name=EY710860; organism=Citrus sinensis; type=EST; length=915bp
TTCTCGACACGCGCTTCCGGTAAAGAGATAACTAAAGGAGTGTTTATGGA
GAGCACCATGAAGGAGATGAGAGACGGAGCTTCTTTTATCCACTAAACTC
GTATCCACCGTCGAAGGCGGCGTTGAAGACGTCTACGGTGAGGATCGTGC
CACCGAGGACCAGCTCGTCACTCCCTGGACCATCTCTGTTGCCAGCGGGT
ACTGTTTGTTGAGGGATCCACGCCATAACAAAGGGCTTGCTTTCACTGAG
AAAGAGAGGGATGCCCACTACTTGCGCGGCCTTTTGCCTTCTTCTGTTAT
AAGTCAACAACTTCAGGAGAAGAAATTGATGATCAGCATCAGACAATATG
AAGTTGCGCTGCAAAAATATGTGGCCATGATGGAACTTGAGGAGAGAAAT
GAAAGACTGTTCTATAAGCTACTGATTGATAATGTTGAGGAACTTCTCCC
AGTCGTTTACACTCCAACTGTTGGTGAAGCTTGCCAGAAATATGGAAGTA
TCTTCAGGCGTCCTCAGGGTCTATACATAAGTCTGAAAGAGAAGGGAAAG
ATTCTTGAGGTTTTGAAGAACTGGCCTGAAAGGAGTATTCAGGTTATTGT
TGTCACAGATGGTGAGCGCATTTTGGGACTTGGGGATCTTGGCTGTCAGG
GGATGGGAATTCCTGGTGGGAAATTGGCTTTGTACACAGCACTTGGAGGC
ATTCGTCCCTCAACATGTTTGCCCATACCGGTGATGTGGGGACAACAATG
AGCAATTGTTGGAAGATGATTTTTACATTGGCCTCCACAGAGGAGGGCAC
CCGGACGGAATATGCTGACTTCTAGAGGATTCCTGAGTGGTGGCAAGCAA
AATTAGGAAAAAAGGTCCTATCCGTTTGAGGATTCCCAACCACATGTTTT
GGAGTGCTGCGAATA
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