EY710860

Overview
NameEY710860
Unique NameEY710860
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length915
Libraries
Library NameType
Sweet orange fruit, development stadium (3 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAOH_NEOFR (Malic enzyme, hydrogenosomal OS=Neocallimastix frontalis PE=1 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 2.208e-42
Identity = 84/182 (46.15%), Postives = 118/182 (64.84%), Query Frame = 2 
Query:  182 ISVASGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            ++  +G  +L DP+ NKG AFT  E+D   +RGL+P    S + Q K+   ++ +    L+K++ +  L+ RNE L+YK++++N  EL P++YTP VGEACQK+  IF + +G+Y S  ++G++  V  NWP   + VIVVTDG RILGLGDLG  GM IP GKL LY   GGI P   LPI
Sbjct:   42 VTKKTGLDILNDPKLNKGSAFTADEKDRLGIRGLVPPRPQSLEAQYKRCKTNLDKISDPLEKFIYLNHLQNRNETLYYKMILENFVELAPIIYTPVVGEACQKFHKIFTQTRGMYFSTADRGQMSAVAANWPYDDVDVIVVTDGSRILGLGDLGAGGMQIPIGKLTLYVCGGGINPRNVLPI 223
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_ENT38 (NAD-dependent malic enzyme OS=Enterobacter sp. (strain 638) GN=sfcA PE=3 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 3.396e-42
Identity = 82/178 (46.07%), Postives = 126/178 (70.79%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+ +ER +  L GLLP  V + + Q ++  I  + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPVLLEFPLLNKGSAFSAEERSSFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRSRGVFISYENRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193

HSP 2 Score: 23.8682 bits (50), Expect = 3.396e-42
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN+QLL D  Y+G
Sbjct:  198 GTNNQQLLTDPLYMG 212
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO12_PHOPR (NAD-dependent malic enzyme 2 OS=Photobacterium profundum GN=sfcA2 PE=3 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 3.396e-42
Identity = 79/178 (44.38%), Postives = 125/178 (70.22%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL +   NKG AF+ +ER    L GLLP+++ S   QE++     + +   + K++ +  +++ NE L+Y+L+ +++EE++P++YTPTVG AC+++ +I+RR +GL++   +KG I+++L N   + +++IV+TDGERILGLGD G  GMGIP GKLALYTA GGI P+  LPI
Sbjct:   13 AGPTLLENALLNKGSAFSPEERQNFSLMGLLPAAIESITEQEERAYSQYQTFNDDMDKHIYLRNIQDTNETLYYRLIDNHIEEMMPIIYTPTVGAACEQFSNIYRRGRGLFLGYPDKGNIVDILNNAARQDVKIIVITDGERILGLGDQGIGGMGIPIGKLALYTACGGINPANTLPI 190

HSP 2 Score: 23.0978 bits (48), Expect = 3.396e-42
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN QLL D  Y+G
Sbjct:  195 GTNNTQLLSDPMYMG 209
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_ERWCT (NAD-dependent malic enzyme OS=Erwinia carotovora subsp. atroseptica GN=sfcA PE=3 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 8.391e-42
Identity = 85/178 (47.75%), Postives = 126/178 (70.79%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AFTE+ER    L GLLP +V + + Q ++     ++++  ++K+V +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+IS   +  I ++L+N  +++++VIVVTDGERILGLGD G  GMGIP GKLALYTA GGI P+  LP+
Sbjct:   16 AGPILLEFPLLNKGSAFTEEERADFNLAGLLPEAVETIEEQAERAWRQYQEFKHDIEKHVYLRNIQDTNETLFYRLLDGHLNEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNRANIDDMLQNATKQNVKVIVVTDGERILGLGDQGIGGMGIPIGKLALYTACGGISPAYTLPV 193
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_SALG2 (NAD-dependent malic enzyme OS=Salmonella gallinarum (strain 287/91 / NCTC 13346) GN=sfcA PE=3 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 9.789e-42
Identity = 82/178 (46.07%), Postives = 124/178 (69.66%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++  +  + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++L+N P  +I+VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPVLLEFPLLNKGSAFSAEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193

HSP 2 Score: 24.2534 bits (51), Expect = 9.789e-42
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN+QLL D  Y+G
Sbjct:  198 GTNNQQLLNDPLYMG 212
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_KLEP7 (NAD-dependent malic enzyme OS=Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) GN=sfcA PE=3 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 9.789e-42
Identity = 81/178 (45.51%), Postives = 124/178 (69.66%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++  I  + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +++VIVVTDGERILGLGD G  GMGIP GKL+LYT  GGI P+  LPI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERSNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLIGNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYQNRHNLDDILQNVPNHNVKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTTCGGISPAYTLPI 193

HSP 2 Score: 25.0238 bits (53), Expect = 9.789e-42
Identity = 9/15 (60.00%), Postives = 12/15 (80.00%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN+QLL+D  Y+G
Sbjct:  198 GTNNQQLLDDPLYMG 212
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_SALTY (NAD-dependent malic enzyme OS=Salmonella typhimurium GN=sfcA PE=3 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 1.275e-41
Identity = 82/178 (46.07%), Postives = 124/178 (69.66%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++  +  + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++L+N P  +I+VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193

HSP 2 Score: 24.2534 bits (51), Expect = 1.275e-41
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN+QLL D  Y+G
Sbjct:  198 GTNNQQLLNDPLYMG 212
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_SALTI (NAD-dependent malic enzyme OS=Salmonella typhi GN=sfcA PE=3 SV=2)

HSP 1 Score: 167.548 bits (423), Expect = 1.275e-41
Identity = 82/178 (46.07%), Postives = 124/178 (69.66%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++  +  + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++L+N P  +I+VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193

HSP 2 Score: 24.2534 bits (51), Expect = 1.275e-41
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN+QLL D  Y+G
Sbjct:  198 GTNNQQLLNDPLYMG 212
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_SALSV (NAD-dependent malic enzyme OS=Salmonella schwarzengrund (strain CVM19633) GN=sfcA PE=3 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 1.275e-41
Identity = 82/178 (46.07%), Postives = 124/178 (69.66%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++  +  + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++L+N P  +I+VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193

HSP 2 Score: 24.2534 bits (51), Expect = 1.275e-41
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN+QLL D  Y+G
Sbjct:  198 GTNNQQLLNDPLYMG 212
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_SALPB (NAD-dependent malic enzyme OS=Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7) GN=sfcA PE=3 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 1.275e-41
Identity = 82/178 (46.07%), Postives = 124/178 (69.66%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++  +  + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++L+N P  +I+VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193

HSP 2 Score: 24.2534 bits (51), Expect = 1.275e-41
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN+QLL D  Y+G
Sbjct:  198 GTNNQQLLNDPLYMG 212
The following BLAST results are available for this feature:
BLAST of EY710860 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAOH_NEOFR2.208e-4246.15Malic enzyme, hydrogenosomal OS=Neocallimastix fro... [more]
MAO1_ENT383.396e-4246.07NAD-dependent malic enzyme OS=Enterobacter sp. (st... [more]
MAO12_PHOPR3.396e-4244.38NAD-dependent malic enzyme 2 OS=Photobacterium pro... [more]
MAO1_ERWCT8.391e-4247.75NAD-dependent malic enzyme OS=Erwinia carotovora s... [more]
MAO1_SALG29.789e-4246.07NAD-dependent malic enzyme OS=Salmonella gallinaru... [more]
MAO1_KLEP79.789e-4245.51NAD-dependent malic enzyme OS=Klebsiella pneumonia... [more]
MAO1_SALTY1.275e-4146.07NAD-dependent malic enzyme OS=Salmonella typhimuri... [more]
MAO1_SALTI1.275e-4146.07NAD-dependent malic enzyme OS=Salmonella typhi GN=... [more]
MAO1_SALSV1.275e-4146.07NAD-dependent malic enzyme OS=Salmonella schwarzen... [more]
MAO1_SALPB1.275e-4146.07NAD-dependent malic enzyme OS=Salmonella paratyphi... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-702-001-A09-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY710860 ID=EY710860; Name=EY710860; organism=Citrus sinensis; type=EST; length=915bp
TTCTCGACACGCGCTTCCGGTAAAGAGATAACTAAAGGAGTGTTTATGGA
GAGCACCATGAAGGAGATGAGAGACGGAGCTTCTTTTATCCACTAAACTC
GTATCCACCGTCGAAGGCGGCGTTGAAGACGTCTACGGTGAGGATCGTGC
CACCGAGGACCAGCTCGTCACTCCCTGGACCATCTCTGTTGCCAGCGGGT
ACTGTTTGTTGAGGGATCCACGCCATAACAAAGGGCTTGCTTTCACTGAG
AAAGAGAGGGATGCCCACTACTTGCGCGGCCTTTTGCCTTCTTCTGTTAT
AAGTCAACAACTTCAGGAGAAGAAATTGATGATCAGCATCAGACAATATG
AAGTTGCGCTGCAAAAATATGTGGCCATGATGGAACTTGAGGAGAGAAAT
GAAAGACTGTTCTATAAGCTACTGATTGATAATGTTGAGGAACTTCTCCC
AGTCGTTTACACTCCAACTGTTGGTGAAGCTTGCCAGAAATATGGAAGTA
TCTTCAGGCGTCCTCAGGGTCTATACATAAGTCTGAAAGAGAAGGGAAAG
ATTCTTGAGGTTTTGAAGAACTGGCCTGAAAGGAGTATTCAGGTTATTGT
TGTCACAGATGGTGAGCGCATTTTGGGACTTGGGGATCTTGGCTGTCAGG
GGATGGGAATTCCTGGTGGGAAATTGGCTTTGTACACAGCACTTGGAGGC
ATTCGTCCCTCAACATGTTTGCCCATACCGGTGATGTGGGGACAACAATG
AGCAATTGTTGGAAGATGATTTTTACATTGGCCTCCACAGAGGAGGGCAC
CCGGACGGAATATGCTGACTTCTAGAGGATTCCTGAGTGGTGGCAAGCAA
AATTAGGAAAAAAGGTCCTATCCGTTTGAGGATTCCCAACCACATGTTTT
GGAGTGCTGCGAATA
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